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    METHOD FOR PREPARING VARIOUS LACTAM
    21.
    发明申请
    METHOD FOR PREPARING VARIOUS LACTAM 审中-公开

    公开(公告)号：US20180037896A1

    公开(公告)日：2018-02-08

    申请号：US15545311

    申请日：2016-04-11

    Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

    Inventor： Sang Yup Lee ,  Tong Un Chae ,  Chan Woo Song

    IPC: C12N15/79 ,  C08G69/14 ,  A61K35/742 ,  C12N15/52

    Abstract: The present disclosure relates to a recombinant microorganism having a lactam production capacity from an omega-amino acid, into which a gene encoding a beta-alanine coenzyme A transferase on a microorganism which has an omega-amino acid biosynthetic metabolic pathway inherently or an omega-amino acid biosynthetic metabolic pathway is introduced, and a method for producing a variety of lactams and omega-amino acyl-CoAs using the same.The recombinant microorganism and the method for producing the lactam according to the present disclosure are useful in producing a variety of lactams such as propiolactam, 2-pyrrolidone, valerolactam, caprolactam, and heptanolactam from a variety of omega-amino acids.

    Synthesis-regulating sRNA and method for preparing same
    23.
    发明授权
    Synthesis-regulating sRNA and method for preparing same 有权
    合成调节sRNA及其制备方法

    公开(公告)号：US09388417B2

    公开(公告)日：2016-07-12

    申请号：US14371557

    申请日：2013-01-11

    Applicant: Korea Advanced Institute of Science and Technology

    Inventor： Sang Yup Lee ,  Dokyun Na ,  Seung Min Yoo

    IPC: C12N15/63 ,  C12N1/21 ,  C12N15/113 ,  C12P7/22 ,  C12P13/22 ,  C12N15/70 ,  C12P13/00

    CPC classification number: C12N15/1138 ,  C12N15/111 ,  C12N15/63 ,  C12N15/67 ,  C12N15/70 ,  C12N2310/11 ,  C12N2310/13 ,  C12N2310/18 ,  C12N2310/3519 ,  C12N2310/531 ,  C12P7/22 ,  C12P13/001 ,  C12P13/225

    Abstract: The present invention relates to a novel customized sRNA that reduces gene expression in prokaryotic cells, a preparation method thereof, and the use thereof, and more particularly to a synthetic sRNA comprising an Hfq binding site, derived from the sRNA of any one of MicC, SgrS and MicF, and a region that base-pairs with the target gene mRNA, and to a preparation method thereof and the use thereof. The synthetic sRNA according to the invention has an advantage in that the degree of inhibition of the target gene can be controlled by regulating the ability of the synthetic sRNA to bind to the mRNA of the target gene. The use of the synthetic sRNA that regulates the expression of the target gene makes it possible to effectively construct a recombinant microorganism without using a conventional gene deletion method and to reduce the expression of the target gene, and thus the synthetic sRNA is useful for the production of recombinant microorganisms. Also, the synthetic sRNA can be quickly applied to various strains, and thus is very suitable for the measurement of metabolic capabilities of strains and the selection of the most suitable strain. In addition, recombinant microorganisms, which are obtained by metabolic flux manipulation using the synthetic sRNA and produce tyrosine or cadaverine with high efficiency, are useful in the drug and industrial fields. In other words, the use of the sRNA according to the present invention can make it easy to select target genes whose expression is to be inhibited for the highly efficient production of metabolites. Accordingly, the synthetic sRNA can be used to construct recombinant strains for efficient production of various metabolites and to establish efficient methods for production of various metabolites, and thus is highly useful.

    Abstract translation: 本发明涉及减少原核细胞中基因表达的新型定制的sRNA，其制备方法及其用途，更具体地涉及包含衍生自MicC中任一种的sRNA的Hfq结合位点的合成sRNA， SgrS和MicF，以及与靶基因mRNA碱基配对的区域及其制备方法及其用途。 根据本发明的合成sRNA具有以下优点：靶基因的抑制程度可以通过调节合成sRNA结合目标基因的mRNA的能力来控制。 调节靶基因表达的合成sRNA的使用使得可以有效地构建重组微生物而不使用常规的基因缺失方法并降低靶基因的表达，因此合成的sRNA可用于生产 的重组微生物。 此外，合成的sRNA可以快速应用于各种菌株，因此非常适合于测定菌株的代谢能力和选择最合适的菌株。 此外，通过使用合成sRNA的代谢通量操作获得的重组微生物以高效率产生酪氨酸或尸胺，可用于药物和工业领域。 换句话说，根据本发明的sRNA的使用可以使得能够容易地选择其表达被抑制用于代谢物的高效生产的靶基因。 因此，合成的sRNA可用于构建用于有效生产各种代谢物的重组菌株，并建立生产各种代谢物的有效方法，因此是非常有用的。

    Recombinant microorganism having increased ability to produce hydrophobic material and cell-membrane engineering method for preparation thereof
    24.
    发明授权
    Recombinant microorganism having increased ability to produce hydrophobic material and cell-membrane engineering method for preparation thereof 有权

    公开(公告)号：US12134793B2

    公开(公告)日：2024-11-05

    申请号：US17515614

    申请日：2021-11-01

    Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

    Inventor： Sang Yup Lee ,  Dongsoo Yang ,  Seon Young Park

    IPC: C12P23/00 ,  C07K14/245 ,  C12N1/20 ,  C12N9/04 ,  C12N9/10 ,  C12N9/90 ,  C12P17/16

    Abstract: Disclosed are a recombinant microorganism for producing a hydrophobic material, which is subjected to cell-membrane engineering in order to be imparted with at least one characteristic among an increase in a cell-membrane area, an increase in formation and secretion of an outer membrane vesicle, and an increase in formation of an inner membrane vesicle, and a cell-membrane engineering method for preparation thereof, whereby an insoluble hydrophobic material can be produced with high efficiency, the recombinant microorganism for high-efficiency production of carotenoids or violacein analogues is useful for producing natural pigments, antioxidants, antibiotics, cosmetic additives, anticancer agents, food additives, or nutritional supplements, and the natural pigment production technology developed herein achieves a great increase in production ability. Therefore, the present invention is effective at preparing a recombinant strain for efficient production of a variety of industrially and medically useful metabolites and at establishing an efficient preparation method.

    NOVEL MALONYL-COA BIOSENSOR BASED ON TYPE III POLYKETIDE SYNTHASE AND USE THEREOF
    26.
    发明申请
    NOVEL MALONYL-COA BIOSENSOR BASED ON TYPE III POLYKETIDE SYNTHASE AND USE THEREOF 有权

    公开(公告)号：US20210277428A1

    公开(公告)日：2021-09-09

    申请号：US16477897

    申请日：2018-08-30

    Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

    Inventor： Sang Yup Lee ,  Dongsoo YANG

    IPC: C12P7/22 ,  C12N15/63 ,  C12N9/10 ,  C12P17/06 ,  C12P7/42 ,  C12Q1/00 ,  C12Q1/02 ,  C12Q1/48

    Abstract: The present invention relates to a recombinant microorganism for malonyl-CoA detection in which a type III polyketide synthase-encoding gene is inserted in the genome or in which a recombinant vector containing the gene is introduced; a method of screening a malonyl-CoA production-inducing substance using the recombinant microorganism; a method of screening a gene which is involved in increased malonyl-CoA production; and a method comprising knocking down the gene, screened by the method, in a microorganism, thus increasing the production of malonyl-CoA in the microorganism, and producing a useful substance in the microorganism using malonyl-CoA as a precursor. The use of the biosensor according to the present invention provides single-step signal generation, utilization in various microorganisms, utilization in self-fluorescent microorganisms, a simple construction method, and a simple screening method. In addition, when the present invention is combined with high-throughput screening, it has advantages in that strains having increased malonyl-CoA producing ability can be screened very easily and rapidly (˜3 days) and can be applied directly to the malonyl-CoA-based production of useful compounds.

    NOVEL SYNTHESIS-REGULATING SRNA AND METHOD FOR PREPARING SAME
    30.
    发明申请
    NOVEL SYNTHESIS-REGULATING SRNA AND METHOD FOR PREPARING SAME 有权
    新型合成调节SRNA及其制备方法

    公开(公告)号：US20140377752A1

    公开(公告)日：2014-12-25

    申请号：US14371557

    申请日：2013-01-11

    Applicant: Korea Advanced Institute of Science and Technology

    Inventor： Sang Yup Lee ,  Dokyun Na ,  Seung Min Yoo

    IPC: C12N15/113 ,  C12P13/00 ,  C12N15/70 ,  C12P13/22

    CPC classification number: C12N15/1138 ,  C12N15/111 ,  C12N15/63 ,  C12N15/67 ,  C12N15/70 ,  C12N2310/11 ,  C12N2310/13 ,  C12N2310/18 ,  C12N2310/3519 ,  C12N2310/531 ,  C12P7/22 ,  C12P13/001 ,  C12P13/225

    Abstract: The present invention relates to a novel customized sRNA that reduces gene expression in prokaryotic cells, a preparation method thereof, and the use thereof, and more particularly to a synthetic sRNA comprising an Hfq binding site, derived from the sRNA of any one of MicC, SgrS and MicF, and a region that base-pairs with the target gene mRNA, and to a preparation method thereof and the use thereof. The synthetic sRNA according to the invention has an advantage in that the degree of inhibition of the target gene can be controlled by regulating the ability of the synthetic sRNA to bind to the mRNA of the target gene. The use of the synthetic sRNA that regulates the expression of the target gene makes it possible to effectively construct a recombinant microorganism without using a conventional gene deletion method and to reduce the expression of the target gene, and thus the synthetic sRNA is useful for the production of recombinant microorganisms. Also, the synthetic sRNA can be quickly applied to various strains, and thus is very suitable for the measurement of metabolic capabilities of strains and the selection of the most suitable strain. In addition, recombinant microorganisms, which are obtained by metabolic flux manipulation using the synthetic sRNA and produce tyrosine or cadaverine with high efficiency, are useful in the drug and industrial fields. In other words, the use of the sRNA according to the present invention can make it easy to select target genes whose expression is to be inhibited for the highly efficient production of metabolites. Accordingly, the synthetic sRNA can be used to construct recombinant strains for efficient production of various metabolites and to establish efficient methods for production of various metabolites, and thus is highly useful.

    Abstract translation: 本发明涉及减少原核细胞中基因表达的新型定制的sRNA，其制备方法及其用途，更具体地涉及包含衍生自MicC中任一种的sRNA的Hfq结合位点的合成sRNA， SgrS和MicF，以及与靶基因mRNA碱基配对的区域及其制备方法及其用途。 根据本发明的合成sRNA具有以下优点：靶基因的抑制程度可以通过调节合成sRNA结合目标基因的mRNA的能力来控制。 调节靶基因表达的合成sRNA的使用使得可以有效地构建重组微生物而不使用常规的基因缺失方法并降低靶基因的表达，因此合成的sRNA可用于生产 的重组微生物。 此外，合成的sRNA可以快速应用于各种菌株，因此非常适合于测定菌株的代谢能力和选择最合适的菌株。 此外，通过使用合成sRNA的代谢通量操作获得的重组微生物以高效率产生酪氨酸或尸胺，可用于药物和工业领域。 换句话说，根据本发明的sRNA的使用可以使得能够容易地选择其表达被抑制用于代谢物的高效生产的靶基因。 因此，合成的sRNA可用于构建用于有效生产各种代谢物的重组菌株，并建立生产各种代谢物的有效方法，因此是非常有用的。

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