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公开(公告)号：US06723549B2
公开(公告)日：2004-04-20
申请号：US08841636
申请日：1997-04-30
申请人： Arja Miettinen-Oinonen , John Londesborough , Jari Vehmaanperä , Heli Haakana , Arja Mäntylä , Raija Lanto , Minna Elovainio , Vesa Joutsjoki , Marja Paloheimo , Pirkko Suominen
发明人： Arja Miettinen-Oinonen , John Londesborough , Jari Vehmaanperä , Heli Haakana , Arja Mäntylä , Raija Lanto , Minna Elovainio , Vesa Joutsjoki , Marja Paloheimo , Pirkko Suominen
IPC分类号： C12N942
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： Genes encoding novel cellulases, and a gene encoding a protein that facilitates the action of such novel cellulases, the novel cellulases and a protein that facilitates the action of such cellulases, and enzyme preparations containing such proteins are described. The native hosts and the culture medium of said hosts containing said novel cellulases are also disclosed. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.
摘要翻译： 编码新型纤维素酶的基因和编码促进这种新型纤维素酶作用的蛋白质的基因，新型纤维素酶和促进这种纤维素酶作用的蛋白质，以及含有这种蛋白质的酶制剂。 还公开了含有所述新型纤维素酶的所述宿主的天然宿主和培养基。 这些蛋白质在纺织和洗涤剂行业以及纸浆和造纸工业中特别有用。
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公开(公告)号：US20130065810A2
公开(公告)日：2013-03-14
申请号：US12799639
申请日：2010-04-28
申请人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo
发明人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo
CPC分类号： C12N9/58 , C11D3/386 , C11D3/38681
摘要： The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence of the mature Fe_RF6318 enzyme having an amino acid sequence of SEQ ID NO: 15. The serine protease is obtainable from Fusarium equiseti, more preferably from the deposited strain CBS 119568. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2521 comprising the nucleotide sequence SEQ ID NO:9 deposited in E. coli RF7664 under accession number DSM 22171 and plasmid pALK2529 comprising the full-length gene SEQ ID NO: 10 deposited in E. coli RF7800 under accession number DSM 22172. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译： 本发明涉及真菌丝氨酸蛋白酶，其包含具有SEQ ID NO：15的氨基酸序列的成熟Fe_RF6318酶的氨基酸序列。丝氨酸蛋白酶可从镰刀菌（Fusarium equiseti）获得，更优选来自沉积菌株 CBS 119568。还公开了编码所述蛋白酶的核酸序列，例如质粒pALK2521，其包含以保藏号DSM 22171保藏在大肠杆菌RF7664中的核苷酸序列SEQ ID NO：9和质粒pALK2529，质粒pALK2529包含全长基因SEQ ID NO： 10，以保藏号DSM 22172保藏在大肠杆菌RF7800中。所述蛋白酶可用作适用于洗涤剂组合物中的酶制剂，并用于处理纤维，用于处理羊毛，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于 任何涉及蛋白质物质修饰，降解或去除的应用。
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公开(公告)号：US08603795B2
公开(公告)日：2013-12-10
申请号：US12799639
申请日：2010-04-28
申请人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo
发明人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo
IPC分类号： C12N9/64
CPC分类号： C12N9/58 , C11D3/386 , C11D3/38681
摘要： The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence of the mature Fe_RF6318 enzyme having an amino acid sequence of SEQ ID NO: 15. The serine protease is obtainable from Fusarium equiseti, more preferably from the deposited strain CBS 119568. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2521 comprising the nucleotide sequence SEQ ID NO:9 deposited in E. coli RF7664 under accession number DSM 22171 and plasmid pALK2529 comprising the full-length gene SEQ ID NO: 10 deposited in E. coli RF7800 under accession number DSM 22172. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译： 本发明涉及真菌丝氨酸蛋白酶，其包含具有SEQ ID NO：15的氨基酸序列的成熟Fe_RF6318酶的氨基酸序列。丝氨酸蛋白酶可从镰刀菌（Fusarium equiseti）获得，更优选来自沉积菌株 CBS 119568。还公开了编码所述蛋白酶的核酸序列，例如质粒pALK2521，其包含以保藏号DSM 22171保藏在大肠杆菌RF7664中的核苷酸序列SEQ ID NO：9和质粒pALK2529，质粒pALK2529包含全长基因SEQ ID NO： 10，以保藏号DSM 22172保藏在大肠杆菌RF7800中。所述蛋白酶可用作适用于洗涤剂组合物中的酶制剂，并用于处理纤维，用于处理羊毛，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于 任何涉及蛋白质物质修饰，降解或去除的应用。
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公开(公告)号：US20110003729A1
公开(公告)日：2011-01-06
申请号：US12799639
申请日：2010-04-28
申请人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo
发明人： Kari Juntunen , Leena Valtakari , Susanna Makinen , Jarno Kallio , Jari Vehmaanpera , Pentti Ojapalo , Marja Paloheimo
CPC分类号： C12N9/58 , C11D3/386 , C11D3/38681
摘要： The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence of the mature Fe_RF6318 enzyme having an amino acid sequence of SEQ ID NO: 15. The serine protease is obtainable from Fusarium equiseti, more preferably from the deposited strain CBS 119568. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2521 comprising the nucleotide sequence SEQ ID NO:9 deposited in E. coli RF7664 under accession number DSM 22171 and plasmid pALK2529 comprising the full-length gene SEQ ID NO: 10 deposited in E. coli RF7800 under accession number DSM 22172. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译： 本发明涉及真菌丝氨酸蛋白酶，其包含具有SEQ ID NO：15的氨基酸序列的成熟Fe_RF6318酶的氨基酸序列。丝氨酸蛋白酶可从镰刀菌（Fusarium equiseti）获得，更优选来自沉积菌株 CBS 119568。还公开了编码所述蛋白酶的核酸序列，例如质粒pALK2521，其包含以保藏号DSM 22171保藏在大肠杆菌RF7664中的核苷酸序列SEQ ID NO：9和质粒pALK2529，质粒pALK2529包含全长基因SEQ ID NO： 10，以保藏号DSM 22172保藏在大肠杆菌RF7800中。所述蛋白酶可用作适用于洗涤剂组合物中的酶制剂，并用于处理纤维，用于处理羊毛，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于 任何涉及蛋白质物质修饰，降解或去除的应用。
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公开(公告)号：US20060246566A1
公开(公告)日：2006-11-02
申请号：US11119526
申请日：2005-04-29
申请人： Jari Vehmaanpera , Terhi Puranen , Leena Valtakari , Jarno Kallio , Marika Alapuranen , Marja Paloheimo , Pentti Ojapalo
发明人： Jari Vehmaanpera , Terhi Puranen , Leena Valtakari , Jarno Kallio , Marika Alapuranen , Marja Paloheimo , Pentti Ojapalo
IPC分类号： C12N9/00
CPC分类号： C12N9/2437 , C12Y302/01004 , C12Y302/01091
摘要： The present invention provides novel cellulase fusion proteins, preparations of cellulase fusion proteins and compositions of cellulase fusion proteins. The present invention further provides cellulase expression vectors, host cells expressing cellulase and methods for preparing such vectors and cells. Uses of cellulases, cellulase preparations and cellulase compositions in the textile, detergent, pulp and paper industries are also provided.
摘要翻译： 本发明提供新型纤维素酶融合蛋白，纤维素酶融合蛋白的制备物和纤维素酶融合蛋白的组合物。 本发明还提供了纤维素酶表达载体，表达纤维素酶的宿主细胞和用于制备这种载体和细胞的方法。 还提供了纤维素酶，纤维素酶制剂和纤维素酶组合物在纺织品，洗涤剂，纸浆和造纸工业中的用途。
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公开(公告)号：US08945900B2
公开(公告)日：2015-02-03
申请号：US13283891
申请日：2011-10-28
CPC分类号： C12N9/58 , C11D3/38609 , C11D3/38618
摘要： The present invention relates to fungal serine protease variants, which comprise an amino acid substitution of valine at position 208 of the parent Fusarium equiseti Fe_RF6318 serine protease, wherein the position of the substitution corresponds to the amino acid sequence of the mature Fe_RF6318 enzyme defined in SEQ ID NO:2. The variants have improved thermal stability and/or detergent stability compared to the parent Fe_RF6318 enzyme. Preferably the substitution is V208I and more preferably the variants comprise additional amino acid changes which further increase the stability. Also disclosed are nucleic acid sequences encoding said protease variants as well as recombinant vectors and host cells for the production of the variants. The serine protease variants are applicable in laundry and dish-washing detergent compositions, in treating fibers, in treating wool, in treating hair, in treating leather, in treating food or feed, or in any application involving modification, degradation or removal of proteinaceous material.
摘要翻译： 本发明涉及真菌丝氨酸蛋白酶变体，其包含母本镰刀菌属Fe_RF6318丝氨酸蛋白酶的208位的缬氨酸的氨基酸取代，其中取代的位置对应于SEQ ID NO：1中定义的成熟Fe_RF6318酶的氨基酸序列 编号：2。 与母体Fe_RF6318酶相比，变体具有改善的热稳定性和/或洗涤剂稳定性。 优选地，取代是V208I，更优选地，变体包含进一步增加稳定性的另外的氨基酸变化。 还公开了编码所述蛋白酶变体的核酸序列以及用于产生变体的重组载体和宿主细胞。 丝氨酸蛋白酶变体可用于洗衣和洗碟洗涤剂组合物，处理纤维，处理羊毛，处理毛发，处理皮革，处理食品或饲料，或涉及任何涉及蛋白质物质的改性，降解或去除的应用 。
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公开(公告)号：US20120252064A1
公开(公告)日：2012-10-04
申请号：US13433984
申请日：2012-03-29
IPC分类号： C12N9/58 , C12N1/15 , D06M16/00 , C12S11/00 , A23J3/34 , C11D3/386 , C11D17/00 , C12P21/06 , C12N15/57 , C12N15/80 , C12N9/96
CPC分类号： C11D3/386 , C11D3/2065 , C11D11/0017 , C11D11/0023 , C12N9/58 , C12Y304/21
摘要： The present invention is related to a fungal serine protease enzyme, which said enzyme has serine protease activity and comprises an amino acid sequence of Malbranchea ALKO4122 mature protease as defined in SEQ ID NO:18 or an amino acid sequence having at least 66% identity to the amino acid sequence of SEQ ID NO:18. Also disclosed is an isolated nucleic acid molecule, comprising a polynucleotide sequence which encodes a fungal serine protease enzyme, nucleic acid sequences encoding said protease, a host cell and a process of producing a polypeptide having serine protease activity. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, wool, hair, leather, or silk, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
摘要翻译： 本发明涉及真菌丝氨酸蛋白酶，其中所述酶具有丝氨酸蛋白酶活性，并且包含如SEQ ID NO：18所定义的Malbranchea ALKO4122成熟蛋白酶的氨基酸序列或与SEQ ID NO：18所定义的氨基酸序列具有至少66％同一性的氨基酸序列 SEQ ID NO：18的氨基酸序列。 还公开了分离的核酸分子，其包含编码真丝丝氨酸蛋白酶的多核苷酸序列，编码所述蛋白酶的核酸序列，宿主细胞和产生具有丝氨酸蛋白酶活性的多肽的方法。 所述蛋白酶可用作适用于洗涤剂组合物和用于处理纤维，羊毛，毛发，皮革或丝绸，用于处理食品或饲料，或用于涉及蛋白质材料的改性，降解或去除的任何应用的酶制剂。
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公开(公告)号：US07741093B2
公开(公告)日：2010-06-22
申请号：US11119526
申请日：2005-04-29
申请人： Jari Vehmaanperä , Terhi Puranen , Leena Valtakari , Jarno Kallio , Marika Alapuranen , Marja Paloheimo , Pentti Ojapalo
发明人： Jari Vehmaanperä , Terhi Puranen , Leena Valtakari , Jarno Kallio , Marika Alapuranen , Marja Paloheimo , Pentti Ojapalo
CPC分类号： C12N9/2437 , C12Y302/01004 , C12Y302/01091
摘要： The present invention provides novel cellulase fusion proteins, preparations of cellulase fusion proteins and compositions of cellulase fusion proteins. The present invention further provides cellulase expression vectors, host cells expressing cellulase and methods for preparing such vectors and cells. Uses of cellulases, cellulase preparations and cellulase compositions in the textile, detergent, pulp and paper industries are also provided.
摘要翻译： 本发明提供新型纤维素酶融合蛋白，纤维素酶融合蛋白的制备物和纤维素酶融合蛋白的组合物。 本发明还提供了纤维素酶表达载体，表达纤维素酶的宿主细胞和用于制备这种载体和细胞的方法。 还提供了纤维素酶，纤维素酶制剂和纤维素酶组合物在纺织品，洗涤剂，纸浆和造纸工业中的使用。
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29.发明授权Method for determining amounts of polynucleotide sequences present in cell or tissue samples 有权确定存在于细胞或组织样品中的多核苷酸序列的量的方法公开(公告)号：US09416401B2
公开(公告)日：2016-08-16
申请号：US10451640
申请日：2002-01-10
CPC分类号： C12Q1/6816 , C12Q2600/158 , C12Q2565/627 , C12Q2565/518 , C12Q2537/143
摘要： The invention is related to a method and test kits for quantitative determination of polynucleotide amounts present in a sample. The test kit comprises organized pools with polynucleotide probes having distinct sizes and optionally provided with tracer tags or primer tags. The probes are allowed to hybridize with affinity tagged analyte polynucleotides from the sample. The result is hybrids, which can be recovered on a separation aiding tool provided with the pair of the affinity tag. After the quantitative release of the probes, the probes are either directly recorded, or if primer tagged, they are amplified and optionally provided with a tracer tag before recording. The invention provides a sensitive and quantitative determination of the amount polynucleotides present in a cell or tissue sample and allows a quantitative assessment of variations in the amounts of polynucleotides as a response to inherent changes or due to external stimuli.
摘要翻译： 本发明涉及用于定量测定样品中存在的多核苷酸量的方法和测试试剂盒。 测试试剂盒包括具有不同大小的多核苷酸探针的有组织的池，并且任选地具有示踪剂标签或引物标签。 允许探针与来自样品的亲和标记的分析物多核苷酸杂交。 结果是混合物，其可以在具有一对亲和标签的分离辅助工具上回收。 探针的定量释放后，直接记录探针，或者如果引物标记，则它们被扩增，并且在记录之前可选地提供示踪剂标签。 本发明提供对存在于细胞或组织样品中的多核苷酸的量的敏感和定量的测定，并允许定量评估多核苷酸的量的变化，作为对固有变化或由于外部刺激的反应。
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公开(公告)号：US20120107905A1
公开(公告)日：2012-05-03
申请号：US13283891
申请日：2011-10-28
CPC分类号： C12N9/58 , C11D3/38609 , C11D3/38618
摘要： The present invention relates to fungal serine protease variants, which comprise an amino acid substitution of valine at position 208 of the parent Fusarium equiseti Fe_RF6318 serine protease, wherein the position of the substitution corresponds to the amino acid sequence of the mature Fe_RF6318 enzyme defined in SEQ ID NO:2. The variants have improved thermal stability and/or detergent stability compared to the parent Fe_RF6318 enzyme. Preferably the substitution is V208I and more preferably the variants comprise additional amino acid changes which further increase the stability. Also disclosed are nucleic acid sequences encoding said protease variants as well as recombinant vectors and host cells for the production of the variants. The serine protease variants are applicable in laundry and dish-washing detergent compositions, in treating fibers, in treating wool, in treating hair, in treating leather, in treating food or feed, or in any application involving modification, degradation or removal of proteinaceous material.
摘要翻译： 本发明涉及真菌丝氨酸蛋白酶变体，其包含母本镰刀菌属Fe_RF6318丝氨酸蛋白酶的208位的缬氨酸的氨基酸取代，其中取代的位置对应于SEQ ID NO：1中定义的成熟Fe_RF6318酶的氨基酸序列 编号：2。 与母体Fe_RF6318酶相比，变体具有改善的热稳定性和/或洗涤剂稳定性。 优选地，取代是V208I，更优选地，变体包含进一步增加稳定性的另外的氨基酸变化。 还公开了编码所述蛋白酶变体的核酸序列以及用于产生变体的重组载体和宿主细胞。 丝氨酸蛋白酶变体可用于洗衣和洗碟洗涤剂组合物，处理纤维，处理羊毛，处理毛发，处理皮革，处理食品或饲料，或涉及任何涉及蛋白质物质的改性，降解或去除的应用 。
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