Fungal serine protease and use thereof
    6.
    发明申请
    Fungal serine protease and use thereof 有权
    真菌丝氨酸蛋白酶及其用途

    公开(公告)号:US20110028375A1

    公开(公告)日:2011-02-03

    申请号:US12799638

    申请日:2010-04-28

    摘要: The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence the mature Fa_RF7182 enzyme having an amino acid sequence of SEQ ID NO: 18. The serine protease is obtainable from Fusarium acuminatum, more preferably from the deposited strain CBS 124084. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2530 comprising the nucleotide sequence SEQ ID NO:12 deposited in Escherichia coli RF7803 under accession number DSM 22208 and plasmid pALK2531 comprising the full-length gene SEQ ID NO: 13 deposited in E. coli RF7879 under accession number DSM 22209, as well as fungal hosts, such as Trichoderm. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.

    摘要翻译: 本发明涉及真菌丝氨酸蛋白酶,其包含具有SEQ ID NO:18的氨基酸序列的成熟Fa_RF7182酶的氨基酸序列。丝氨酸蛋白酶可从尖锐镰孢获得,更优选来自保藏菌株CBS 还公开了编码所述蛋白酶的核酸序列,例如包含以保藏号DSM 22208沉积在大肠杆菌RF7803中的核苷酸序列SEQ ID NO:12的质粒pALK2530和保藏的全长基因SEQ ID NO:13的质粒pALK2531 在保藏号DSM 22209的大肠杆菌RF7879中,以及真菌宿主如Trichoderm。 所述蛋白酶可用作适用于洗涤剂组合物和用于处理纤维,用于处理毛发,用于处理皮革,用于处理食品或饲料,或用于任何涉及蛋白质材料的改性,降解或去除的任何应用的酶制剂。

    Fungal serine protease and use thereof
    7.
    发明授权
    Fungal serine protease and use thereof 有权
    真菌丝氨酸蛋白酶及其用途

    公开(公告)号:US08609390B2

    公开(公告)日:2013-12-17

    申请号:US12799638

    申请日:2010-04-28

    IPC分类号: C12N9/68

    摘要: The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence the mature Fa_RF7182 enzyme having an amino acid sequence of SEQ ID NO: 18. The serine protease is obtainable from Fusarium acuminatum, more preferably from the deposited strain CBS 124084. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2530 comprising the nucleotide sequence SEQ ID NO:12 deposited in Escherichia coli RF7803 under accession number DSM 22208 and plasmid pALK2531 comprising the full-length gene SEQ ID NO: 13 deposited in E. coli RF7879 under accession number DSM 22209, as well as fungal hosts, such as Trichoderm. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.

    摘要翻译: 本发明涉及真菌丝氨酸蛋白酶,其包含具有SEQ ID NO:18的氨基酸序列的成熟Fa_RF7182酶的氨基酸序列。丝氨酸蛋白酶可从尖锐镰孢获得,更优选来自保藏菌株CBS 还公开了编码所述蛋白酶的核酸序列,例如包含以保藏号DSM 22208沉积在大肠杆菌RF7803中的核苷酸序列SEQ ID NO:12的质粒pALK2530和保藏的全长基因SEQ ID NO:13的质粒pALK2531 在保藏号DSM 22209的大肠杆菌RF7879中,以及真菌宿主如Trichoderm。 所述蛋白酶可用作适用于洗涤剂组合物和用于处理纤维,用于处理毛发,用于处理皮革,用于处理食品或饲料,或用于任何涉及蛋白质材料的改性,降解或去除的任何应用的酶制剂。

    Enzyme fusion proteins and their use
    8.
    发明申请
    Enzyme fusion proteins and their use 有权
    酶融合蛋白及其用途

    公开(公告)号:US20070244020A1

    公开(公告)日:2007-10-18

    申请号:US11404065

    申请日:2006-04-13

    摘要: Cellulase fusion proteins comprising an endoglucanase core region and a heterologous cellulose binding domain are described. The fusion proteins may be produced by recombinant techniques using appropriate polynucleotides, expressing vectors and host cells. The fusion proteins and enzyme preparations thereof are useful in treating cellulosic material, such as textile material, and they are particularly useful in biostoning denim or in biofinishing fabrics and garments. In addition the fusion proteins may be used in pulp and paper industry, oil extraction from plants, detergent compositions, or for improving the quality of animal feed.

    摘要翻译: 描述了包含内切葡聚糖酶核心区域和异源纤维素结合结构域的纤维素酶融合蛋白。 可以通过使用合适的多核苷酸,表达载体和宿主细胞的重组技术产生融合蛋白。 融合蛋白及其酶制剂可用于处理纤维素材料如纺织材料,并且它们特别适用于生物结构牛仔布或生物整理织物和服装。 此外,融合蛋白质可用于纸浆和造纸工业,从植物提取油,洗涤剂组合物或用于改善动物饲料的质量。

    Novel Fungal Protease and Use Thereof
    10.
    发明申请
    Novel Fungal Protease and Use Thereof 有权
    新型真菌蛋白酶及其用途

    公开(公告)号:US20130065810A2

    公开(公告)日:2013-03-14

    申请号:US12799639

    申请日:2010-04-28

    摘要: The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence of the mature Fe_RF6318 enzyme having an amino acid sequence of SEQ ID NO: 15. The serine protease is obtainable from Fusarium equiseti, more preferably from the deposited strain CBS 119568. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2521 comprising the nucleotide sequence SEQ ID NO:9 deposited in E. coli RF7664 under accession number DSM 22171 and plasmid pALK2529 comprising the full-length gene SEQ ID NO: 10 deposited in E. coli RF7800 under accession number DSM 22172. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.

    摘要翻译: 本发明涉及真菌丝氨酸蛋白酶,其包含具有SEQ ID NO:15的氨基酸序列的成熟Fe_RF6318酶的氨基酸序列。丝氨酸蛋白酶可从镰刀菌(Fusarium equiseti)获得,更优选来自沉积菌株 CBS 119568。还公开了编码所述蛋白酶的核酸序列,例如质粒pALK2521,其包含以保藏号DSM 22171保藏在大肠杆菌RF7664中的核苷酸序列SEQ ID NO:9和质粒pALK2529,质粒pALK2529包含全长基因SEQ ID NO: 10,以保藏号DSM 22172保藏在大肠杆菌RF7800中。所述蛋白酶可用作适用于洗涤剂组合物中的酶制剂,并用于处理纤维,用于处理羊毛,用于处理毛发,用于处理皮革,用于处理食品或饲料,或用于 任何涉及蛋白质物质修饰,降解或去除的应用。