摘要:
Initiation of coupling between an input disk and a clutch disk at a maximum rotational coupling force without making relative rotation between the input disk and the clutch disk is enabled only when the output shaft is placed in a single engageable point within a normal reciprocal angular range of the output shaft. When a load applied to the output shaft is equal to or greater than a predetermined value, the input disk and the clutch disk are decoupled from one another, so that the relative rotation is made between the input disk and the clutch disk.
摘要:
A novel feline cytokine protein having the activity to enhance the cytotoxic activity of feline cytotoxic T lymphocytes, a DNA sequence coding for said protein, a recombinant DNA for expressing said protein, an expression vector comprising said recombinant DNA, a transformant which is transformed with said expression vector, a process for preparing said protein by culturing said transformant, and an antibody against said protein are provided. The novel feline cytokine protein of the present invention is a heterologous dimer comprising FLAF p35 and FLAF p40 and can be used for treating feline infectious diseases such as feline herpes virus type 1 (FHV-1) or feline calicivirus (FCV).
摘要:
Herein disclosed is a retractable wiper device which comprises: a first crank connected for forward and backward rotations to the reduction output shaft of a motor; and a second crank rotatably supported on the first crank such that it is kept in association at a predetermined bent angle with the first crank, while the first crank is being rotated forward, and in general association with the first crank while the first crank is being rotated backward. When in the forward rotation of the first crank, a stopper is always pushed to keep its engagement with the first crank thereby maintaining the aforementioned predetermined bent angle by two overlapped cams which are associated with each other at a predetermined play angle. When in the backward rotation of the first crank, on the other hand, the push of the stopper by the two cams is released in the vicinity of the top turn position of a wiper blade, and the portion of the stopper in the vicinity of the pushed portion by the cams is relieved in the forward direction of the first crank. As a result, the first and second cranks are generally linear in the vicinity of the top turn position of the wiper blade.
摘要:
A Factor X (hereinafter referred to as “FX”) with a high activity is provided. The present invention relates to a method for efficiently preparing a recombinant, two-chain FX which comprises intervening glycosylation at such an amino acid sequence that is essential for glycosylation in FX to thereby allow for expression of a recombinant FX with no glycosylation, and the recombinant FX with no glycosylation obtained by said method.
摘要:
A Factor X (hereinafter referred to as “FX”) with a high activity is provided. The present invention relates to a method for efficiently preparing a recombinant, two-chain FX which comprises intervening glycosylation at such an amino acid sequence that is essential for glycosylation in FX to thereby allow for expression of a recombinant FX with no glycosylation, and the recombinant FX with no glycosylation obtained by said method.
摘要:
The present invention provides for a process for preparing a recombinant human thrombin. A process for preparing a recombinant human thrombin which comprises: (1) obtaining a transfectant cell producing a human prothrombin by introducing an expression vector, wherein a gene fragment coding for a human prothrombin gene is incorporated, into an animal cell; (2) purifying a human prothrombin from the culture of the transfectant cell above by an anion exchanger; (3) converting the purified human prothrombin into a human thrombin by subjecting said human prothrombin to the action of ecarin; and (4) purifying the human thrombin from the solution after treatment with ecarin by an affinity method using benzamidine and a cation exchanger, and human thrombin obtained by said process, and a CHO cell that produces human prothrombin.
摘要:
A gene encoding a production amount-potentiating factor is introduced into an animal cell to transform the cell. Alternatively, a protein production gene and the gene encoding the production amount-potentiating factor are introduced into the animal cell to transform the cell. Herein, as the production amount potentiating factor, there is used a factor having caspase activity inhibiting activity and/or protein biosynthesis activity potentiating action, for example, baculovirus P35. Further, the animal cell is cultured by a culturing method under a condition that apoptosis is not induced, so that a protein is mass-produced.
摘要:
A novel feline cytokine protein having the activity to enhance the cytotoxic activity of feline cytotoxic T lymphocytes, a DNA sequence coding for said protein, a recombinant DNA for expressing said protein, an expression vector comprising said recombinant DNA, a transformant which is transformed with said expression vector, a process for preparing said protein by culturing said transformant, and an antibody against said protein are provided. The novel feline cytokine protein of the present invention is a heterologous dimer comprising FLAF p35 and FLAF p40 and can be used for treating feline infectious diseases such as feline herpes virus type 1 (FHV-1) or feline calicivirus (FCV).
摘要:
A novel feline cytokine protein having the activity to enhance the cytotoxic activity of feline cytotoxic T lymphocytes, a DNA sequence coding for said protein, a recombinant DNA for expressing said protein, an expression vector comprising said recombinant DNA, a transformant which is transformed with said expression vector, a process for preparing said protein by culturing said transformant, and an antibody against said protein are provided. The novel feline cytokine protein of the present invention is a heterologous dimer comprising FLAF p35 and FLAF p40 and can be used for treating feline infectious diseases such as feline herpes virus type 1 (FHV-1) or feline calicivirus (FCV).
摘要:
The invention is designed to calibrate a low-level optical power by a substitution method using a pulse generating section in which a light-shielding disk having a sectorial opening, as a component capable of accurate optical attenuation, is rotated in a light beam to generate an intermittent pulse signal having a duty ratio corresponding to the size of the opening, and the pulse signal is averaged to obtain an optical attenuation equal to the duty ratio. More specifically, while an object to be calibrated is set in a given standard state, a first pulse train having a duty ratio d1=Tw.sub.1 /Tf.sub.1 is input to the object, and an average optical power value A of an output from the object is measured and stored. Subsequently, a second pulse train having a duty ratio d2=Tw.sub.2 /Tf.sub.2 is input to the object, and the object is adjusted such that an average optical power value of an output from the object becomes the value A. Proper calibration can be performed by assigning the optical attenuation of the adjusted object to d1/d2.