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公开(公告)号：US07091011B2
公开(公告)日：2006-08-15
申请号：US10378202
申请日：2003-03-03
申请人： Michael G. Bramucci , Robert Dicosimo , Robert Fallon , John E. Gavagan , Frank Herkes , Lech Wilczek
发明人： Michael G. Bramucci , Robert Dicosimo , Robert Fallon , John E. Gavagan , Frank Herkes , Lech Wilczek
摘要： This invention relates to a process for the preparation of a 3-hydroxycarboxylic acid from a 3-hydroxynitrile. More specifically, 3-hydroxyvaleronitrile is converted to 3-hydroxyvaleric acid in high yield at up to 100% conversion, using as an enzyme catalyst 1) nitrile hydratase activity and amidase activity or 2) nitrilase activity of a microbial cell. 3-Hydroxyvaleric acid is used as a substitute for ε-caprolactone in the preparation of highly branched copolyester.
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公开(公告)号：US06562603B2
公开(公告)日：2003-05-13
申请号：US09921260
申请日：2001-08-02
申请人： Michael G. Bramucci , Robert Dicosimo , Robert Fallon , John E. Gavagan , Frank Herkes , Lech Wilczek
发明人： Michael G. Bramucci , Robert Dicosimo , Robert Fallon , John E. Gavagan , Frank Herkes , Lech Wilczek
IPC分类号： C12P742
摘要： This invention relates to a process for the preparation of a 3-hydroxycarboxylic acid from a 3-hydroxynitrile. More specifically, 3-hydroxyvaleronitrile is converted to 3-hydroxyvaleric acid in high yield at up to 100% conversion, using as an enzyme catalyst 1) nitrile hydratase activity and amidase activity or 2) nitrilase activity of a microbial cell. 3-Hydroxyvaleric acid is used as a substitute for &egr;-caprolactone in the preparation of highly branched copolyester.
摘要翻译： 本发明涉及一种由3-羟基腈制备3-羟基羧酸的方法。 更具体地，使用酶催化剂1）腈水合酶活性和酰胺酶活性或2）微生物细胞的腈水解酶活性，将3-羟基戊腈以高产率转化为3-羟基戊酸。 在制备高支化共聚酯时，使用3-羟基戊酸作为ε-己内酯的替代物。
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23.发明授权Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans 有权丁醇脱氢酶从细菌无色杆菌xylosoxidans公开(公告)号：US08188250B2
公开(公告)日：2012-05-29
申请号：US12430356
申请日：2009-04-27
摘要： From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.
摘要翻译： 从分离自环境样品的细菌菌株中，在含有1-丁醇作为碳源的培养基中富集后，鉴定出具有丁醇脱氢酶活性的新酶。 该酶可将丁醛转化为1-丁醇，异丁醛至异丁醇，以及2-丁酮转化为2-丁醇，因此可用于生产这些底物的重组微生物宿主中丁醇的生物合成。 将编码基因命名为sadB，从鉴定为无色杆菌木糖苷的分离株的菌株中分离出来。
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24.发明申请FERMENTIVE PRODUCTION OF ISOBUTANOL USING HIGHLY ACTIVE KETOL-ACID REDUCTOISOMERASE ENZYMES 有权使用高活性酮酸降低脂肪酶的人造丝生产ISOBUTMOL公开(公告)号：US20080261230A1
公开(公告)日：2008-10-23
申请号：US12103844
申请日：2008-04-16
CPC分类号： C12Q1/689 , C12N9/0006 , C12P7/42
摘要： Methods for the fermentative production of isobutanol is provided by the fermentative growth of a recombinant microorganism expressing a highly active ketol-acid reductoisomerase enzyme in addition to other enzymes required for conversion of glucose to isobutanol.
摘要翻译： 通过发酵生长表达高活性酮酸酸还原异构酶的重组微生物，除了将葡萄糖转化为异丁醇所需的其它酶外，还提供了异丁醇发酵生产方法。
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公开(公告)号：US07285410B2
公开(公告)日：2007-10-23
申请号：US11585036
申请日：2006-10-23
申请人： Michael G. Bramucci , Robert DiCosimo , Sarita Chauhan , Robert D. Fallon , John E. Gavagan , Mark S. Payne
发明人： Michael G. Bramucci , Robert DiCosimo , Sarita Chauhan , Robert D. Fallon , John E. Gavagan , Mark S. Payne
CPC分类号： C12N9/88 , C12N9/80 , C12P7/40 , C12P41/006
摘要： A method has been developed to prepare (E)- and (Z)-2-methyl-2-butenoic acids (2M2BA) from a mixture of (E,Z)-2-methyl-2-butenenitriles (2M2BN) by the regioselective hydrolysis of (E)-2M2BN to (E)-2-methyl-2-butenoic acid (2M2BA) using enzyme catalysts having either a nitrilase activity or a combination of nitrile hydratase and amidase activities. The method provides high yields without significant conversion of (Z)-2M2BN to (Z)-2M2BA. The regioselective hydrolysis of (E)-2M2BN to (E)-2M2BA makes possible the facile separation of (E)-2M2BA from (Z)-2M2BN or (Z)-2-methyl-2-butenamide (2M2BAm), and the subsequent conversion of (Z)-2M2BN or (Z)-2M2BAm to (Z)-2M2BA.
摘要翻译： 已经开发了一种由（E，Z）-2-甲基-2-丁烯腈（2M 2 BN）的混合物通过区域选择性制备（E） - 和（Z）-2-甲基-2-丁烯酸（2M 2 BA）的方法 使用具有腈水解酶活性或腈水合酶和酰胺酶活性的组合的酶催化剂将（E）-2M2BN水解成（E）-2-甲基-2-丁烯酸（2M2BA）。 该方法提供高产率而不显着转化（Z）-2M2BN至（Z）-2M2BA。 （E）-2M2BN至（E）-2M2BA的区域选择性水解使（E）-2M2BA与（Z）-2M2BN或（Z）-2-甲基-2-丁烯酰胺（2M2BAm）的容易分离成为可能， 随后将（Z）-2M2BN或（Z）-2M2BAm转化为（Z）-2M2BA。
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公开(公告)号：US07220562B2
公开(公告)日：2007-05-22
申请号：US11338471
申请日：2006-01-24
申请人： Michael G. Bramucci , Robert DiCosimo , Sarita Chauhan , Robert D. Fallon , John E. Gavagan , Mark S. Payne
发明人： Michael G. Bramucci , Robert DiCosimo , Sarita Chauhan , Robert D. Fallon , John E. Gavagan , Mark S. Payne
IPC分类号： C12P13/00 , C12P7/40 , C12Q1/34 , C12N11/14 , C12N11/02 , C12N1/20 , C12N9/78 , C12N15/00 , C07H21/04 , C07K14/00
CPC分类号： C12N9/88 , C12N9/80 , C12P7/40 , C12P41/006
摘要： A method has been developed to prepare (E)- and (Z)-2-methyl-2-butenoic acids (2M2BA) from a mixture of (E,Z)-2-methyl-2-butenenitriles (2M2BN) by the regioselective hydrolysis of (E)-2M2BN to (E)-2-methyl-2-butenoic acid (2M2BA) using enzyme catalysts having either a nitrilase activity or a combination of nitrile hydratase and amidase activities. The method provides high yields without significant conversion of (Z)-2M2BN to (Z)-2M2BA. The regioselective hydrolysis of (E)-2M2BN to (E)-2M2BA makes possible the facile separation of (E)-2M2BA from (Z)-2M2BN or (Z)-2-methyl-2-butenamide (2M2BAm), and the subsequent conversion of (Z)-2M2BN or (Z)-2M2BAm to (Z)-2M2BA.
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27.发明授权Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans 有权丁醇脱氢酶从细菌无色杆菌xylosoxidans公开(公告)号：US08691540B2
公开(公告)日：2014-04-08
申请号：US13479643
申请日：2012-05-24
IPC分类号： C12P7/16 , C12N9/04 , C12N1/12 , C12N1/13 , C12N1/14 , C12N1/21 , C12N15/56 , C12N1/18 , C12N1/20
摘要： From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.
摘要翻译： 从分离自环境样品的细菌菌株中，在含有1-丁醇作为碳源的培养基中富集后，鉴定出具有丁醇脱氢酶活性的新酶。 该酶可将丁醛转化为1-丁醇，异丁醛至异丁醇，以及2-丁酮转化为2-丁醇，因此可用于生产这些底物的重组微生物宿主中丁醇的生物合成。 将编码基因命名为sadB，从鉴定为无色杆菌木糖苷的分离株的菌株中分离出来。
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公开(公告)号：US07416859B2
公开(公告)日：2008-08-26
申请号：US11069691
申请日：2005-03-01
CPC分类号： C12N15/74
摘要： A plasmid has been isolated from Rhodococcus erythropolis strain AN12 comprising a unique replication protein. The replication protein may be used in a variety of cloning and expression vectors and particularly in shuttle vectors for the expression of heterologous genes in Rhodococcus sp.
摘要翻译： 已经从包含独特复制蛋白的红球菌菌株AN12分离出质粒。 复制蛋白可用于多种克隆和表达载体，特别是用于在红球菌属中表达异源基因的穿梭载体中。
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公开(公告)号：US07138480B2
公开(公告)日：2006-11-21
申请号：US10378201
申请日：2003-03-03
申请人： Michael G. Bramucci , Robert Dicosimo , Robert Fallon , John E. Gavagan , Frank Herkes , Lech Wilczek
发明人： Michael G. Bramucci , Robert Dicosimo , Robert Fallon , John E. Gavagan , Frank Herkes , Lech Wilczek
IPC分类号： C08G63/06
摘要： This invention relates to a process for the preparation of a 3-hydroxycarboxylic acid from a 3-hydroxynitrile. More specifically, 3-hydroxyvaleronitrile is converted to 3-hydroxyvaleric acid in high yield at up to 100% conversion, using as an enzyme catalyst 1) nitrile hydratase activity and amidase activity or 2) nitrilase activity of a microbial cell. 3-Hydroxyvaleric acid is used as a substitute for ε-caprolactone in the preparation of highly branched copolyester.
摘要翻译： 本发明涉及一种由3-羟基腈制备3-羟基羧酸的方法。 更具体地，使用酶催化剂1）腈水合酶活性和酰胺酶活性或2）微生物细胞的腈水解酶活性，将3-羟基戊腈以高产率转化为3-羟基戊酸。 3-羟基戊酸用于制备高支化共聚酯中的ε-己内酯的替代物。
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30.发明授权Temperature sensitive mutant derivatives of the broad host range plasmid pBHR1 失效宽宿主范围质粒pBHR1的温度敏感突变体衍生物公开(公告)号：US07045338B2
公开(公告)日：2006-05-16
申请号：US10378967
申请日：2003-03-04
申请人： Michael G. Bramucci
发明人： Michael G. Bramucci
摘要： The present invention describes a mutant plasmid replication control region having the ability to convey temperature sensitivity to the plasmid on which it resides. The mutant replication control region is based on a similar region isolated from pBRH1. Plasmids containing this rggeplication control region cannot be classed as belonging to any known incompatibility group and thus may co-exist with a broad range of other plasmids in a single host.
摘要翻译： 本发明描述了具有将温度敏感性传递给其所在质粒的突变质粒复制控制区。 突变体复制控制区域基于与pBRH1分离的相似区域。 含有该rggeplication对照区的质粒不能归类为任何已知的不相容性基团，因此可以与单个宿主中的宽范围的其他质粒共存。
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