公开(公告)号：US07091011B2

公开(公告)日：2006-08-15

申请号：US10378202

申请日：2003-03-03

申请人： Michael G. Bramucci ,  Robert Dicosimo ,  Robert Fallon ,  John E. Gavagan ,  Frank Herkes ,  Lech Wilczek

发明人： Michael G. Bramucci ,  Robert Dicosimo ,  Robert Fallon ,  John E. Gavagan ,  Frank Herkes ,  Lech Wilczek

IPC分类号： C12P7/62 ,  C12P13/00

CPC分类号： C12P7/625 ,  C08G63/06 ,  C08G63/85 ,  C12P7/42 ,  C12R1/01 ,  C12R1/40

摘要： This invention relates to a process for the preparation of a 3-hydroxycarboxylic acid from a 3-hydroxynitrile. More specifically, 3-hydroxyvaleronitrile is converted to 3-hydroxyvaleric acid in high yield at up to 100% conversion, using as an enzyme catalyst 1) nitrile hydratase activity and amidase activity or 2) nitrilase activity of a microbial cell. 3-Hydroxyvaleric acid is used as a substitute for ε-caprolactone in the preparation of highly branched copolyester.

公开(公告)号：US06562603B2

公开(公告)日：2003-05-13

申请号：US09921260

申请日：2001-08-02

申请人： Michael G. Bramucci ,  Robert Dicosimo ,  Robert Fallon ,  John E. Gavagan ,  Frank Herkes ,  Lech Wilczek

发明人： Michael G. Bramucci ,  Robert Dicosimo ,  Robert Fallon ,  John E. Gavagan ,  Frank Herkes ,  Lech Wilczek

IPC分类号： C12P742

CPC分类号： C12P7/625 ,  C08G63/06 ,  C08G63/85 ,  C12P7/42 ,  C12R1/01 ,  C12R1/40

摘要： This invention relates to a process for the preparation of a 3-hydroxycarboxylic acid from a 3-hydroxynitrile. More specifically, 3-hydroxyvaleronitrile is converted to 3-hydroxyvaleric acid in high yield at up to 100% conversion, using as an enzyme catalyst 1) nitrile hydratase activity and amidase activity or 2) nitrilase activity of a microbial cell. 3-Hydroxyvaleric acid is used as a substitute for &egr;-caprolactone in the preparation of highly branched copolyester.

摘要翻译： 本发明涉及一种由3-羟基腈制备3-羟基羧酸的方法。 更具体地，使用酶催化剂1）腈水合酶活性和酰胺酶活性或2）微生物细胞的腈水解酶活性，将3-羟基戊腈以高产率转化为3-羟基戊酸。 在制备高支化共聚酯时，使用3-羟基戊酸作为ε-己内酯的替代物。

公开(公告)号：US07138480B2

公开(公告)日：2006-11-21

申请号：US10378201

申请日：2003-03-03

申请人： Michael G. Bramucci ,  Robert Dicosimo ,  Robert Fallon ,  John E. Gavagan ,  Frank Herkes ,  Lech Wilczek

发明人： Michael G. Bramucci ,  Robert Dicosimo ,  Robert Fallon ,  John E. Gavagan ,  Frank Herkes ,  Lech Wilczek

IPC分类号： C08G63/06

CPC分类号： C12P7/625 ,  C08G63/06 ,  C08G63/85 ,  C12P7/42 ,  C12R1/01 ,  C12R1/40

摘要： This invention relates to a process for the preparation of a 3-hydroxycarboxylic acid from a 3-hydroxynitrile. More specifically, 3-hydroxyvaleronitrile is converted to 3-hydroxyvaleric acid in high yield at up to 100% conversion, using as an enzyme catalyst 1) nitrile hydratase activity and amidase activity or 2) nitrilase activity of a microbial cell. 3-Hydroxyvaleric acid is used as a substitute for ε-caprolactone in the preparation of highly branched copolyester.

摘要翻译： 本发明涉及一种由3-羟基腈制备3-羟基羧酸的方法。 更具体地，使用酶催化剂1）腈水合酶活性和酰胺酶活性或2）微生物细胞的腈水解酶活性，将3-羟基戊腈以高产率转化为3-羟基戊酸。 3-羟基戊酸用于制备高支化共聚酯中的ε-己内酯的替代物。

公开(公告)号：US07285410B2

公开(公告)日：2007-10-23

申请号：US11585036

申请日：2006-10-23

申请人： Michael G. Bramucci ,  Robert DiCosimo ,  Sarita Chauhan ,  Robert D. Fallon ,  John E. Gavagan ,  Mark S. Payne

发明人： Michael G. Bramucci ,  Robert DiCosimo ,  Sarita Chauhan ,  Robert D. Fallon ,  John E. Gavagan ,  Mark S. Payne

IPC分类号： C12N1/20 ,  C12N9/78 ,  C12N9/88 ,  C12N15/00 ,  C12Q1/34 ,  C07K14/00 ,  C07H21/04

CPC分类号： C12N9/88 ,  C12N9/80 ,  C12P7/40 ,  C12P41/006

摘要： A method has been developed to prepare (E)- and (Z)-2-methyl-2-butenoic acids (2M2BA) from a mixture of (E,Z)-2-methyl-2-butenenitriles (2M2BN) by the regioselective hydrolysis of (E)-2M2BN to (E)-2-methyl-2-butenoic acid (2M2BA) using enzyme catalysts having either a nitrilase activity or a combination of nitrile hydratase and amidase activities. The method provides high yields without significant conversion of (Z)-2M2BN to (Z)-2M2BA. The regioselective hydrolysis of (E)-2M2BN to (E)-2M2BA makes possible the facile separation of (E)-2M2BA from (Z)-2M2BN or (Z)-2-methyl-2-butenamide (2M2BAm), and the subsequent conversion of (Z)-2M2BN or (Z)-2M2BAm to (Z)-2M2BA.

摘要翻译： 已经开发了一种由（E，Z）-2-甲基-2-丁烯腈（2M 2 BN）的混合物通过区域选择性制备（E） - 和（Z）-2-甲基-2-丁烯酸（2M 2 BA）的方法 使用具有腈水解酶活性或腈水合酶和酰胺酶活性的组合的酶催化剂将（E）-2M2BN水解成（E）-2-甲基-2-丁烯酸（2M2BA）。 该方法提供高产率而不显着转化（Z）-2M2BN至（Z）-2M2BA。 （E）-2M2BN至（E）-2M2BA的区域选择性水解使（E）-2M2BA与（Z）-2M2BN或（Z）-2-甲基-2-丁烯酰胺（2M2BAm）的容易分离成为可能， 随后将（Z）-2M2BN或（Z）-2M2BAm转化为（Z）-2M2BA。

公开(公告)号：US07220562B2

公开(公告)日：2007-05-22

申请号：US11338471

申请日：2006-01-24

申请人： Michael G. Bramucci ,  Robert DiCosimo ,  Sarita Chauhan ,  Robert D. Fallon ,  John E. Gavagan ,  Mark S. Payne

发明人： Michael G. Bramucci ,  Robert DiCosimo ,  Sarita Chauhan ,  Robert D. Fallon ,  John E. Gavagan ,  Mark S. Payne

IPC分类号： C12P13/00 ,  C12P7/40 ,  C12Q1/34 ,  C12N11/14 ,  C12N11/02 ,  C12N1/20 ,  C12N9/78 ,  C12N15/00 ,  C07H21/04 ,  C07K14/00

CPC分类号： C12N9/88 ,  C12N9/80 ,  C12P7/40 ,  C12P41/006

摘要： A method has been developed to prepare (E)- and (Z)-2-methyl-2-butenoic acids (2M2BA) from a mixture of (E,Z)-2-methyl-2-butenenitriles (2M2BN) by the regioselective hydrolysis of (E)-2M2BN to (E)-2-methyl-2-butenoic acid (2M2BA) using enzyme catalysts having either a nitrilase activity or a combination of nitrile hydratase and amidase activities. The method provides high yields without significant conversion of (Z)-2M2BN to (Z)-2M2BA. The regioselective hydrolysis of (E)-2M2BN to (E)-2M2BA makes possible the facile separation of (E)-2M2BA from (Z)-2M2BN or (Z)-2-methyl-2-butenamide (2M2BAm), and the subsequent conversion of (Z)-2M2BN or (Z)-2M2BAm to (Z)-2M2BA.

公开(公告)号：US07198926B2

公开(公告)日：2007-04-03

申请号：US10431965

申请日：2003-05-08

申请人： Michael G. Bramucci ,  Robert Dicosimo ,  John E. Gavagan ,  Mark S. Payne ,  Robert D. Fallon ,  Sarita Chauhan

发明人： Michael G. Bramucci ,  Robert Dicosimo ,  John E. Gavagan ,  Mark S. Payne ,  Robert D. Fallon ,  Sarita Chauhan

IPC分类号： C12P13/00 ,  C12P7/40 ,  C12Q1/34 ,  C12N11/14 ,  C12N11/02 ,  C12N1/20 ,  C12N9/78 ,  C12N15/00 ,  C07H21/04 ,  C07K14/00

CPC分类号： C12N9/88 ,  C12N9/80 ,  C12P7/40 ,  C12P41/006

摘要： A method has been developed to prepare (E)- and (Z)-2-methyl-2-butenoic acids (2M2BA) from a mixture of (E,Z)-2-methyl-2-butenenitriles (2M2BN) by the regioselective hydrolysis of (E)-2M2BN to (E)-2-methyl-2-butenoic acid (2M2BA) using enzyme catalysts having either a nitrilase activity or a combination of nitrile hydratase and amidase activities. The method provides high yields without significant conversion of (Z)-2M2BN to (Z)-2M2BA. The regioselective hydrolysis of (E)-2M2BN to (E)-2M2BA makes possible the facile separation of (E)-2M2BA from (Z)-2M2BN or (Z)-2-methyl-2-butenamide (2M2BAm), and the subsequent conversion of (Z)-2M2BN or (Z)-2M2BAm to (Z)-2M2BA.

公开(公告)号：US08426173B2

公开(公告)日：2013-04-23

申请号：US12110503

申请日：2008-04-28

申请人： Michael G. Bramucci ,  Dennis Flint ,  Edward S. Miller, Jr. ,  Vasantha Nagarajan ,  Natalia Sedkova ,  Manjari Singh ,  Tina K. Van Dyk

发明人： Michael G. Bramucci ,  Dennis Flint ,  Edward S. Miller, Jr. ,  Vasantha Nagarajan ,  Natalia Sedkova ,  Manjari Singh ,  Tina K. Van Dyk

IPC分类号： C12P7/16

CPC分类号： C12N15/74 ,  C12N15/70 ,  C12P7/16 ,  C12Y103/01038 ,  C12Y103/01044 ,  C12Y402/01017 ,  C12Y402/01055 ,  Y02E50/10

摘要： A method for the production of 1-butanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product.

摘要翻译： 公开了通过使用微生物生产宿主发酵生产1-丁醇的方法。 该方法在发酵过程中使用温度降低，导致生产主体对丁醇产物的更强的耐受性。

公开(公告)号：US20120149080A1

公开(公告)日：2012-06-14

申请号：US13162165

申请日：2011-06-16

申请人： VASANTHA NAGARAJAN ,  Michael G. Bramucci

发明人： VASANTHA NAGARAJAN ,  Michael G. Bramucci

IPC分类号： C12P7/16 ,  C12N1/19 ,  C12N1/16

CPC分类号： C12R1/645 ,  C12N1/16 ,  C12N15/01 ,  C12P7/16 ,  C12R1/865 ,  Y02E50/10

摘要： High cell density cultures of yeast were found to have higher tolerance for butanol in the medium. The high cell density yeast cultures had greater survival and higher glucose utilization than cultures with low cell densities. Production of butanol using yeast in high cell density cultures is thus beneficial for improving butanol production.

摘要翻译： 发现酵母的高细胞密度培养物对培养基中的丁醇具有较高的耐受性。 高细胞密度酵母培养物比具有低细胞密度的培养物具有更高的存活和更高的葡萄糖利用率。 因此，在高细胞密度培养物中使用酵母生产丁醇因此有利于改善丁醇生产。

公开(公告)号：US08017364B2

公开(公告)日：2011-09-13

申请号：US11949793

申请日：2007-12-04

申请人： Michael G. Bramucci ,  Helene M. A. Kane ,  Vasantha Nagarajan

发明人： Michael G. Bramucci ,  Helene M. A. Kane ,  Vasantha Nagarajan

IPC分类号： C12P7/16 ,  C12P1/20

CPC分类号： C12R1/01 ,  C12P7/16 ,  Y02E50/10

摘要： Pediococcus bacteria having enhanced tolerance to butanols have been isolated. The bacteria are useful for the fermentative production of butanol.

摘要翻译： 已经分离了对丁醇具有增强耐受性的细菌。 该细菌可用于发酵生产丁醇。

公开(公告)号：US07541173B2

公开(公告)日：2009-06-02

申请号：US11761497

申请日：2007-06-12

申请人： Michael G. Bramucci ,  Vasantha Nagarajan ,  Natalia Sedkova ,  Manjari Singh

发明人： Michael G. Bramucci ,  Vasantha Nagarajan ,  Natalia Sedkova ,  Manjari Singh

IPC分类号： C12N1/20 ,  C12N1/21 ,  C12P7/16

CPC分类号： C12N15/01 ,  C12N1/20 ,  C12P7/16 ,  C12P7/26 ,  C12R1/225 ,  Y02E50/10

摘要： Lactobacillus bacteria having enhanced tolerance to butanols have been isolated. The bacteria are useful for the fermentive production of butanol. New methods for the isolation of butanol tolerant Lactobacillus are also provided.

摘要翻译： 已经分离了对丁醇具有增强耐受性的乳杆菌属细菌。 该细菌可用于发酵生产丁醇。 还提供了用于分离耐丁醇乳杆菌的新方法。