公开(公告)号：US06225078B1

公开(公告)日：2001-05-01

申请号：US09122591

申请日：1998-07-27

申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： C12Q126

CPC分类号： C12Q1/004 ,  C12Q1/006

摘要： The invention provides a method for quantitative measurement of a substrate with high accuracy by electrochemically oxidizing an electron mediator which has been reduced by enzyme reaction thereby determining the substrate concentration based on a current flowing during electrochemical oxidation from which adverse effects of an easy-to-oxidize substance on the oxidation process have been minimized. The quantitating method in accordance with the present invention comprises a first step for causing a substrate contained in a sample to react with a specific oxidoreductase to the substrate in the presence of an electron mediator in oxidized state, and a second step for electrochemically reducing the electron mediator in oxidized state which remains non-reduced by the enzyme reaction in the first step, thereby obtaining a current flowing during electrochemical reduction.

摘要翻译： 本发明提供了一种用于通过电化学氧化已经通过酶反应降低的电子介体来高精度地定量测量衬底的方法，从而基于在电化学氧化期间流动的电流来确定衬底浓度，从而容易地 - 在氧化过程中氧化物质已被最小化。 根据本发明的定量方法包括第一步骤，用于在电子介体的氧化态存在下使包含在样品中的底物与特定的氧化还原酶与底物反应，第二步用于电化学还原电子 在第一步中通过酶反应保持未还原的氧化态介体，从而获得在电化学还原过程中流动的电流。

公开(公告)号：US6077408A

公开(公告)日：2000-06-20

申请号：US002368

申请日：1998-01-02

申请人： Yoshiko Miyamoto ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Yoshiko Miyamoto ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N27/327 ,  C12Q1/00 ,  C12Q1/26 ,  G01N27/26

CPC分类号： C12Q1/001 ,  C12Q1/26

摘要： Disclosed is a biosensor facilitating high accuracy quantification of a specific component in a sample solution with no adverse influence of solid substances. The biosensor comprises an electrically insulating base plate, an electrode system comprising a working electrode and a counter electrode formed on the base plate, a reaction layer containing at least one enzyme disposed on the electrode system, and an anionic filter formed on the reaction layer for inhibiting permeation of solid components.

摘要翻译： 公开了一种促进样品溶液中特定组分的高精度定量的生物传感器，其不受固体物质的不利影响。 生物传感器包括电绝缘基板，包括在基板上形成的工作电极和对电极的电极系统，包含设置在电极系统上的至少一个酶的反应层和形成在反应层上的阴离子过滤器 抑制固体组分的渗透。

公开(公告)号：US5906921A

公开(公告)日：1999-05-25

申请号：US159686

申请日：1998-09-24

申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： C12Q1/00 ,  G01N33/543 ,  C12Q1/26 ,  C12Q1/54

CPC分类号： C12Q1/004 ,  G01N33/5438 ,  Y10S435/817

摘要： A biosensor is disclosed for application to a method for quantitative measurement of a substrate comprising a first step of causing a substrate contained in a sample to react with an oxidoreductase specific to the substrate in the presence of an electron mediator in oxidized state, a second step of applying a potential to a working electrode for reducing the electron mediator in oxidized state that remains not reduced in the first step, and a third step of measuring a reduction current flowing across the working electrode and a counter electrode. The biosensor comprises an electrically insulating base plate, an electrode system having a working electrode and a counter electrode formed on the base plate, and a reaction layer which is formed on or in the vicinity of the electrode system and contains at least an oxidoreductase and an electron mediator, the counter electrode including at least a reductant of a redox compound or an electrolytically oxidizable metal.

摘要翻译： 公开了用于定量测量基材的方法的生物传感器，其包括第一步骤，使第一步骤使得包含在样品中的底物在氧化态的电子介体存在下与特定于氧化还原酶的氧化还原酶反应，第二步 在第一步骤中向工作电极施加电位以还原氧化态的电子介体，其保持不减少;以及第三步骤，测量流过工作电极和对电极的还原电流。 生物传感器包括电绝缘基板，具有工作电极和形成在基板上的对电极的电极系统和形成在电极系统上或附近的反应层，至少含有氧化还原酶和 电子介体，所述对电极至少包含氧化还原化合物或电解可氧化金属的还原剂。

公开(公告)号：US5565085A

公开(公告)日：1996-10-15

申请号：US428040

申请日：1995-04-25

申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno

发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno

IPC分类号： G01N27/416 ,  C12Q1/00 ,  G01N27/30 ,  G01N27/327 ,  G01N27/26

CPC分类号： C12Q1/001 ,  Y10S435/817

摘要： A biosensor for quantifying a specific compound has a reaction layer containing at least an enzyme, and an electrode system having a working electrode and a counter electrode formed on an insulating base plate; the biosensor detects the specific compound contained in a sample on the basis of an electrochemical response. In quantifying, the working electrode and the counter electrode are short-circuited before the voltage is applied therebetween. The short-circuiting eliminates measuring errors that may occur due to nonuniform dissolution of the reaction layer in a sample, and achieves highly reliable quantification of a specific compound.

摘要翻译： 用于定量特定化合物的生物传感器具有至少含有酶的反应层和在绝缘基板上形成有工作电极和对电极的电极系统; 生物传感器基于电化学响应来检测样品中包含的特定化合物。 在量化之前，工作电极和对电极在施加电压之前短路。 短路消除了由于反应层在样品中的不均匀溶解而可能发生的测量误差，并且实现了特定化合物的高度可靠的定量。

公开(公告)号：US06214612B1

公开(公告)日：2001-04-10

申请号：US08806801

申请日：1997-03-03

申请人： Tomohiro Yamamoto ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Iwata

发明人： Tomohiro Yamamoto ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Iwata

IPC分类号： C12M134

CPC分类号： C12Q1/005 ,  Y10S435/817

摘要： A cholesterol sensor for quantitative determination of cholesterol is provided containing an electrode system and a reaction reagent system. The electrode system contains a measuring electrode such as a carbon electrode and a counter electrode, and the reaction reagent system contains cholesterol dehydrogenase, nicotinamide adenine dinucleotide and an oxidized electron mediator. Electron mediators include ferricyanide, 1,2-naphthoquinone-4-sulfonate, 2,6-dichlorophenol indophenol, dimethylbenzoquinone, 1-methoxy-5-methylphenazinium sulfate, methylene blue, gallocyanine, thionine, phenazine methosulfate and Meldola's blue. Diaphorase, cholesterol esterase and a surfactant may also be present. The electrode system is on an insulating base plate, and the base plate has a covering member containing a groove that is a sample supplying channel which extends from an end of the base plate to the electrode system. A reaction layer containing the reagent system in dry form and a layer of a hydrophilic polymer is provided on the base plate or the covering member, or on both the electrode system and covering member so as to be exposed to the sample supplying channel. During operation, the electron mediator is reduced in conjunction with oxidation of cholesterol in a sample by cholesterol dehydrogenase, and an amount of current required to electrochemically re-oxidize the electron mediator is directly proportional to a quantity of cholesterol present in the sample.

摘要翻译： 提供了用于定量测定胆固醇的胆固醇传感器，其包含电极系统和反应试剂系统。 电极系统含有碳电极和对电极等测定电极，反应试剂系统含有胆固醇脱氢酶，烟酰胺腺嘌呤二核苷酸和氧化电子介体。 电子介体包括铁氰化物，1,2-萘醌-4-磺酸盐，2,6-二氯苯酚靛酚，二甲基苯并醌，1-甲氧基-5-甲基吩嗪鎓硫酸盐，亚甲基蓝，花青素，硫堇，吩嗪甲硫酸盐和梅尔多拉蓝。 也可以存在心律变性酶，胆固醇酯酶和表面活性剂。 电极系统在绝缘基板上，基板具有包含作为从基板的端部延伸到电极系统的样品供给路径的槽的覆盖部件。 含有干式试剂体系和亲水性聚合物层的反应层设置在基板或覆盖部件上，或电极系统和覆盖部件上，以暴露于样品供应通道。 在操作期间，电子介体与胆固醇脱氢酶在样品中的胆固醇氧化一起被还原，并且电化学再氧化电子介体所需的电流量与样品中存在的胆固醇量成正比。

公开(公告)号：US5651869A

公开(公告)日：1997-07-29

申请号：US605391

申请日：1996-02-22

申请人： Toshihiko Yoshioka ,  Shin Ikeda ,  Shiro Nankai

发明人： Toshihiko Yoshioka ,  Shin Ikeda ,  Shiro Nankai

IPC分类号： C12Q1/00 ,  G01N27/26 ,  C12Q1/64

CPC分类号： C12Q1/006 ,  C12Q1/004 ,  Y10S435/817

摘要： The biosensor of this invention includes an electrically insulating substrate; an electrode system formed on the substrate which includes a working electrode and a counter electrode; and a reaction layer formed on the substrate or above the substrate with a space therebetween. The reaction layer includes a pyranose-oxidizing enzyme.

摘要翻译： 本发明的生物传感器包括电绝缘基板; 形成在所述基板上的包括工作电极和对电极的电极系统; 以及形成在基板上或基板上方的反应层，其间具有空间。 反应层包括吡喃糖氧化酶。

公开(公告)号：US06258254B1

公开(公告)日：2001-07-10

申请号：US09122628

申请日：1998-07-27

申请人： Yoshiko Miyamoto ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Yoshiko Miyamoto ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01F164

CPC分类号： C12Q1/004

摘要： A method using a biosensor is disclosed which facilitates high accuracy quantitation of a substrate by best eliminating adverse effects of red blood cell existing in blood together with the substrate. The biosensor comprises an electrode system including a working electrode and a counter electrode formed on an electrically insulating base plate, and a reaction layer containing at least an oxidoreductase and an electron acceptor, wherein the electron acceptor is a sodium salt.

摘要翻译： 公开了一种使用生物传感器的方法，其通过最好地消除血液中存在于血液中的红细胞与基质一起的不良影响，促进了基质的高精度定量。 生物传感器包括电极系统，其包括在电绝缘基板上形成的工作电极和对电极，以及至少包含氧化还原酶和电子受体的反应层，其中电子受体是钠盐。

公开(公告)号：US5985116A

公开(公告)日：1999-11-16

申请号：US989426

申请日：1997-12-12

申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N27/327 ,  C12M1/00 ,  C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/001

摘要： The biosensor of the present invention has a reaction layer that contains at least an oxidoreductase and an electron acceptor and is formed on an electrode system including a working electrode and a counter electrode on a base plate. The working electrode, the counter electrode, and lead conductors connecting with these electrodes are made of carbon. The counter electrode is formed in a substantially C shape, and the working electrode is arranged in a recess of the substantially C-shaped counter electrode. An insulating layer surrounds the counter electrode and the working electrode. A cover member with a groove is combined with the base plate to define a sample supply channel that is formed therebetween and runs toward the electrode system. The groove extends from one end of the base plate and passes over the electrode system, and the insulating layer extends to an air hole formed in a terminal of the groove of the cover member. This arrangement prevents exposure of part of the lead conductor connecting with the working electrode to the sample solution and development of an error in measurement.

摘要翻译： 本发明的生物传感器具有至少包含氧化还原酶和电子受体的反应层，并且形成在基板上的包括工作电极和对电极的电极系统上。 与这些电极连接的工作电极，对电极和引线导体由碳制成。 对电极形成为大致C形，工作电极配置在基本上为C形的对电极的凹部中。 绝缘层包围对电极和工作电极。 具有凹槽的盖构件与基板结合以限定形成在其间并朝向电极系统延伸的样品供应通道。 凹槽从基板的一端延伸并且经过电极系统，并且绝缘层延伸到形成在盖构件的凹槽的端子中的空气孔。 这样可以防止与工作电极连接的引出导体的一部分暴露于样品溶液，并且产生测量误差。

公开(公告)号：US5575895A

公开(公告)日：1996-11-19

申请号：US452774

申请日：1995-05-30

申请人： Shin Ikeda ,  Mariko Miyahara ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Shin Ikeda ,  Mariko Miyahara ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/004 ,  Y10S435/817

摘要： A biosensor for quantification of a specific component contained in various biological samples comprises an electrically insulating base plate, an electrode system including a working electrode and a counter electrode which are provided on the electrically insulating base plate, a reaction layer formed on the electrode system including at least an oxido-reductase, and an enclosure member having a hollow space constituting a sample supplying channel on the electrically insulating base plate, wherein substantially the whole of the reaction layer is exposed to the hollow space.

摘要翻译： 用于定量各种生物样品中包含的特定成分的生物传感器包括电绝缘基板，包括设置在电绝缘基板上的工作电极和对电极的电极系统，形成在电极系统上的反应层，包括 至少一种氧化还原酶，以及具有中空空间的封闭构件，该中空空间构成电绝缘基板上的样品供应通道，其中基本上整个反应层暴露于中空空间。

公开(公告)号：US06790327B2

公开(公告)日：2004-09-14

申请号：US10023734

申请日：2001-12-21

申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N27327

CPC分类号： C12Q1/004

摘要： An apparatus for determining the concentration of a substrate in a sample solution using an electrode system comprising a working electrode, a counter electrode, and a reaction layer which contains at least an oxidoreductase and an electron mediator and is formed on the electrode system to electrochemically measure a reduced amount of the electron mediator resulting from enzyme reaction in the reaction layer, wherein a third electrode is formed as an interfering substance detecting electrode. A current flowing between the counter electrode and the third electrode is measured which is taken as a positive error. Subsequently, voltage application between the counter electrode and the third electrode is released and a voltage for oxidizing the reduced form electron mediator is applied between the working electrode and the counter electrode to measure a current flowing between the two electrodes.

摘要翻译： 一种用于使用包括工作电极，对电极和至少包含氧化还原酶和电子介体的反应层的电极系统来确定样品溶液中基底的浓度的装置，并且形成在电极系统上以电化学测量 在反应层中由酶反应产生的电子介体的量减少，其中形成第三电极作为干扰物质检测电极。 测量在对电极和第三电极之间流动的电流，其被认为是正误差。 随后，释放对电极和第三电极之间的电压施加，并且在工作电极和对电极之间施加用于氧化还原形式的电子介体的电压，以测量在两个电极之间流动的电流。