公开(公告)号：US20170159090A1

公开(公告)日：2017-06-08

申请号：US15116006

申请日：2015-02-06

Applicant: Novozymes A/S

Inventor： Shiro Fukuyama ,  Keiichi Ayabe

IPC: C12P19/14 ,  C12N9/44 ,  C12N9/34 ,  C12N9/26

CPC classification number: C12P19/14 ,  A23L29/35 ,  C12N9/2402 ,  C12N9/2411 ,  C12N9/2428 ,  C12N9/2457 ,  C12P19/02 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/01041 ,  C13K1/06

Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.

公开(公告)号：US20160040205A1

公开(公告)日：2016-02-11

申请号：US14918114

申请日：2015-10-20

Applicant: NOVOZYMES A/S ,  NOVOZYMES NORTH AMERICA, INC.

Inventor： Shiro Fukuyama ,  Tomoko Matsui ,  Chee Leong Soong ,  Eric Allain ,  Anders Vikso Nielsen ,  Hiroaki Udagawa ,  Ye Liu ,  Junxin Duan ,  Wenping Wu ,  Lene Nonboe Andersen

IPC: C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C12N9/26

CPC classification number: C12P19/14 ,  C07K2319/00 ,  C07K2319/20 ,  C12N9/2414 ,  C12N9/2417 ,  C12N9/242 ,  C12N9/2428 ,  C12N9/96 ,  C12P7/10 ,  C12P7/14 ,  C12P19/02 ,  C12Y302/01001 ,  C12Y302/01003 ,  Y02E50/16

Abstract: The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.

Abstract translation: 本发明涉及包含碳水化合物结合组件氨基酸序列和α-淀粉酶氨基酸序列以及这些多肽的应用的多肽。

公开(公告)号：US20150337277A1

公开(公告)日：2015-11-26

申请号：US14653154

申请日：2013-12-13

Applicant: Novozymes A/S

Inventor： Guillermo Coward Kelly ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/30 ,  C12G3/02 ,  C12P7/06

CPC classification number: C12N9/242 ,  C12C5/004 ,  C12C7/04 ,  C12G3/02 ,  C12N9/2414 ,  C12P7/06 ,  C12P19/14 ,  C12P21/02 ,  C12Y302/01001 ,  Y02E50/17

Abstract: The present disclosure relates to isolated polypeptides having alpha-amylase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides, and method of using polypeptides, including in ethanol production processes.

Abstract translation: 本公开涉及具有α-淀粉酶活性的分离的多肽，编码多肽的多核苷酸，核酸构建体，载体和包含多核苷酸的宿主细胞以及产生多肽的方法，以及使用多肽的方法，包括在乙醇生产过程 。

公开(公告)号：US20240101991A1

公开(公告)日：2024-03-28

申请号：US17936076

申请日：2022-09-28

Applicant: NOVOZYMES A/S

Inventor： Marc Dominique Morant ,  Tomoko Matsui ,  Kirk Matthew Schnorr ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Zhengfang Kang

IPC: C12N9/26 ,  C12N15/81 ,  C12P7/06

CPC classification number: C12N9/2408 ,  C12N15/81 ,  C12P7/06 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/01028

Abstract: The present invention relates to polypeptides having trehalase activity, particularly derived from Talaromyces. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides for the production of ethanol.

公开(公告)号：US11866751B2

公开(公告)日：2024-01-09

申请号：US17260516

申请日：2019-07-22

Applicant: Novozymes A/S

Inventor： Michael Glenn Catlett ,  Monica Tassone ,  Paul Vincent Harris ,  Robert Lyle Osborne ,  Ryoko Kataoka ,  Shiro Fukuyama ,  Tomoko Matsui

IPC: C12P7/06

CPC classification number: C12P7/06 ,  C12P2203/00 ,  C12Y302/01001 ,  C12Y302/01093

Abstract: Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding an alpha-amylase and/or a heterologous polynucleotide encoding a trehalase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms.

公开(公告)号：US20230235367A1

公开(公告)日：2023-07-27

申请号：US17798197

申请日：2021-02-09

Applicant: Novozymes A/S

Inventor： Carsten Andersen ,  Thomas Agersten Poulsen ,  Dorota Nissen ,  Marie Thryøse Kruse ,  Sarah Schultheis Elliott ,  Shiro Fukuyama

IPC: C12P7/10 ,  C12N9/26 ,  C12N15/81 ,  C12N15/52

CPC classification number: C12P7/10 ,  C12N9/2414 ,  C12N15/81 ,  C12N15/52 ,  C12Y302/01001

Abstract: The present invention relates to raw starch hydrolysis and in particular to a raw starch hydrolysis and fermentation process. More particularly the present invention relates to a process of producing a fermentation product from raw starch material, comprising the steps of: (a) saccharifying starch-containing material at a temperature below the initial gelatinization temperature of said starch-containing material; and (b) fermenting with a fermenting organism, wherein step (a) is carried out using at least a variant alpha-amylase comprising a substitution at one or more positions corresponding to positions 196, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 28, 38, 39, 43, 54, 56, 57, 64, 67, 68, 70, 71, 86, 89, 90, 94, 96, 99, 101, 103, 107, 108, 110, 113, 114, 117, 127, 134, 138, 142, 150, 151, 152, 156, 169, 171, 174, 179, 183, 193, 199, 200, 204, 205, 207, 208, 209, 212, 218, 221, 222, 224, 233, 241, 245, 259, 275, 278, 281, 282, 283, 284, 285, 308, 323, 335, 348, 359, 382, 386, 388, 392, 394, 396, 412, 414, 417, 424, 428, 457, 459, 466, 479, 489, 511, 533, 534, 542, 543, 545, 547, 549, 550, 551, 560, 566, 570, 574, 575, 576, 577, 578, 580, 581, 582, 589, 592, 599, 603, 605, 608, 614, 619, or 626 of the polypeptide of SEQ ID NO: 1, wherein the variant has alpha-amylase activity and wherein the variant has at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity, but less than 100% sequence identity, to the polypeptide of SEQ ID NO: 1, and optionally a glucoamylase.

公开(公告)号：US11377648B2

公开(公告)日：2022-07-05

申请号：US17045903

申请日：2019-04-08

Applicant: NOVOZYMES A/S

Inventor： Tomoko Namoto ,  Takashi Nakanishi ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/30 ,  C12P7/06 ,  C12P19/02 ,  C12P19/14 ,  C12N15/52 ,  C12N15/62 ,  C12N15/82 ,  C12N1/16

Abstract: The present invention relates to a hybrid polypeptide having alpha-amylase activity, selected from a first polypeptide sequence comprising a catalytic core, and a second polypeptide sequence comprising a carbohydrate binding module (CBM), wherein (a) the catalytic core is selected from a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to amino acids 20 to 494 of SEQ ID NO: 1 or amino acids 20 to 496 of SEQ ID NO: 1; and (b) the CBM is selected from a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and catalytic domains.

公开(公告)号：US20210139875A1

公开(公告)日：2021-05-13

申请号：US17045903

申请日：2019-04-08

Applicant: NOVOZYMES A/S

Inventor： Tomoko Namoto ,  Takashi Nakanishi ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/30 ,  C12P19/14 ,  C12P19/02 ,  C12N15/82 ,  C12P7/06

Abstract: The present invention relates to a hybrid polypeptide having alpha-amylase activity, selected from a first polypeptide sequence comprising a catalytic core, and a second polypeptide sequence comprising a carbohydrate binding module (CBM), wherein (a) the catalytic core is selected from a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to amino acids 20 to 494 of SEQ ID NO: 1 or amino acids 20 to 496 of SEQ ID NO: 1; and (b) the CBM is selected from a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and catalytic domains.

公开(公告)号：US10584324B2

公开(公告)日：2020-03-10

申请号：US15520567

申请日：2015-10-23

Applicant: NOVOZYMES A/S

Inventor： Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/34 ,  C12P19/14 ,  C12N15/56 ,  C12N1/15 ,  C12P19/02 ,  C12P7/06 ,  C12N9/30 ,  C12N9/44 ,  C12P7/14

Abstract: The present invention relates to glucoamylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20190256878A1

公开(公告)日：2019-08-22

申请号：US16403919

申请日：2019-05-06

Applicant: Novozymes A/S

Inventor： Shiro Fukuyama ,  Keiichi Ayabe

IPC: C12P19/14 ,  C12N9/34 ,  C12P19/02 ,  C12N9/44 ,  A23L29/30 ,  C12N9/24 ,  C13K1/06 ,  C12N9/26

Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.