-
公开(公告)号：US20130236884A1
公开(公告)日：2013-09-12
申请号：US13889046
申请日：2013-05-07
IPC分类号： C12Q1/70
CPC分类号： C12Q1/701 , C12Q1/6888
摘要： Oligonucleotide primers and compositions and kits containing the same for rapid identification of orthopoxviruses by amplification of a segment of viral nucleic acid followed by molecular mass analysis are provided.
摘要翻译： 提供了通过扩增一段病毒核酸，随后进行分子质量分析的寡核苷酸引物和包含其的组合物和试剂盒，用于快速鉴定正痘病毒。
-
公开(公告)号：US08394945B2
公开(公告)日：2013-03-12
申请号：US11683286
申请日：2007-03-07
CPC分类号： C12Q1/689 , C12Q1/6816 , C12Q1/6858 , C12Q2600/156 , C12Q2565/627 , C12Q2537/143
摘要： The present invention provides compositions, kits and methods for rapid identification and quantification of bacteria by molecular mass and base composition analysis.
摘要翻译： 本发明提供通过分子量和碱组成分析快速鉴定和定量细菌的组合物，试剂盒和方法。
-
23.发明授权Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent thereby 有权定义数据库的二级结构和用于确定未知生物标签的身份和地理起源的方法公开(公告)号：US08298760B2
公开(公告)日：2012-10-30
申请号：US11674538
申请日：2007-02-13
申请人： David J. Ecker , Richard H. Griffey , Rangarajan Sampath , Steven A. Hofstadler , John McNeil , Stanley T. Crooke
发明人： David J. Ecker , Richard H. Griffey , Rangarajan Sampath , Steven A. Hofstadler , John McNeil , Stanley T. Crooke
IPC分类号： C12Q1/68
CPC分类号： G06F19/28 , G06F19/00 , G06F19/10 , G06F19/16 , G06F19/18 , G16H50/80 , Y02A90/24 , Y02A90/26
摘要： The present invention relates generally to the field of investigational bioinformatics and more particularly to secondary structure defining databases. The present invention further relates to methods for interrogating a database as a source of molecular masses of known bioagents for comparing against the molecular mass of an unknown or selected bioagent to determine either the identity of the selected bioagent, and/or to determine the origin of the selected bioagent. The identification of the bioagent is important for determining a proper course of treatment and/or irradication of the bioagent in such cases as biological warfare. Furthermore, the determination of the geographic origin of a selected bioagent will facilitate the identification of potential criminal identity.
摘要翻译： 本发明一般涉及研究生物信息学领域，更具体地涉及定义数据库的二级结构。 本发明还涉及询问数据库作为已知生物标签的分子量来源的方法，用于与未知或选择的生物标签的分子量进行比较以确定所选择的生物标签的身份，和/或确定所选生物标记的来源 所选的生物标签。 生物制剂的鉴定对于在诸如生物战争的情况下确定生物制剂的适当治疗过程和/或辐照是重要的。 此外，确定所选择的生物制剂的地理起源将有助于确定潜在的犯罪认同。
-
公开(公告)号：US08288523B2
公开(公告)日：2012-10-16
申请号：US11683254
申请日：2007-03-07
CPC分类号： C12Q1/689 , C12Q1/6816 , C12Q1/6858 , C12Q2600/156 , C12Q2565/627 , C12Q2537/143
摘要： The present invention provides compositions, kits and methods for rapid identification and quantification of bacteria by molecular mass and base composition analysis.
-
公开(公告)号：US08268565B2
公开(公告)日：2012-09-18
申请号：US12326800
申请日：2008-12-02
申请人： David J. Ecker , Richard H. Griffey , Rangarajan Sampath , Stephen A. Hofstadler , John McNeil , Stanley T. Crooke , Lawrence B. Blyn , Thomas A. Hall , Yun Jiang , James C. Hannis , Neill White , Vivek Samant , Mark W. Eshoo , Jared J. Drader
发明人： David J. Ecker , Richard H. Griffey , Rangarajan Sampath , Stephen A. Hofstadler , John McNeil , Stanley T. Crooke , Lawrence B. Blyn , Thomas A. Hall , Yun Jiang , James C. Hannis , Neill White , Vivek Samant , Mark W. Eshoo , Jared J. Drader
CPC分类号： C12Q1/686 , C12Q1/6806 , C12Q1/6844 , C12Q1/6858 , C12Q1/6872 , C12Q1/6888 , C12Q1/689 , C12Q2600/156 , G01N2333/28 , G01N2333/32 , G01N2333/35 , G01N2333/36 , C12Q2563/167 , C12Q2545/101 , C12Q2531/113 , C12Q2565/627 , C12Q2545/113
摘要： The present invention provides methods for rapid forensic analysis of mitochondrial DNA and methods for characterizing heteroplasmy of mitochondrial DNA, which can be used to assess the progression of mitochondrial diseases.
摘要翻译： 本发明提供线粒体DNA的快速法医分析方法和线粒体DNA异质性表征的方法，可用于评估线粒体疾病的进展。
-
26.发明授权公开(公告)号：US08187814B2
公开(公告)日：2012-05-29
申请号：US12684742
申请日：2010-01-08
CPC分类号： C12Q1/6851 , C07H21/04 , C12Q1/6872 , C12Q2545/101
摘要： The present invention provides methods for the quantification of an unknown bioagent in a sample by amplification of nucleic acid of the bioagent, and concurrent amplification of a known quantity of a calibration polynucleotide from which are obtained a bioagent identifying amplicon and a calibration amplicon. Upon molecular mass analysis, mass and abundance data are obtained. The identity of the bioagent is then determined from the molecular mass of the bioagent identifying amplicon and the quantity of the identified bioagent in the sample is determined from the abundance data of the bioagent identifying amplicon and the abundance data of the calibration amplicon.
摘要翻译： 本发明提供了通过扩增生物标记的核酸来定量样品中的未知生物标签的方法，以及同时扩增已知量的校准多核苷酸，从中获得了鉴定扩增子的生物标志物和校准扩增子。 通过分子质量分析，获得质量和丰度数据。 然后根据鉴定扩增子的生物标志物的分子量确定生物标签的身份，并从鉴定扩增子的生物标志物的丰度数据和校准扩增子的丰度数据确定样品中鉴定的生物标记物的量。
-
公开(公告)号：US20120122103A1
公开(公告)日：2012-05-17
申请号：US11683370
申请日：2007-03-07
CPC分类号： C12Q1/689 , C12Q1/6816 , C12Q1/6858 , C12Q2600/156 , C12Q2565/627 , C12Q2537/143
摘要： The present invention provides compositions, kits and methods for rapid identification and quantification of bacteria by molecular mass and base composition analysis.
-
公开(公告)号：US20120122102A1
公开(公告)日：2012-05-17
申请号：US11683360
申请日：2007-03-07
CPC分类号： C12Q1/689 , C12Q1/6816 , C12Q1/6858 , C12Q2600/156 , C12Q2565/627 , C12Q2537/143
摘要： The present invention provides compositions, kits and methods for rapid identification and quantification of bacteria by molecular mass and base composition analysis.
-
公开(公告)号：US08071309B2
公开(公告)日：2011-12-06
申请号：US11930002
申请日：2007-10-30
申请人： David J. Ecker , Richard H. Griffey , Rangarajan Sampath , Steven A. Hofstadler , John McNeil , Stanley T. Crooke , Lawrence Blyn , Ray Ranken , Thomas A. Hall
发明人： David J. Ecker , Richard H. Griffey , Rangarajan Sampath , Steven A. Hofstadler , John McNeil , Stanley T. Crooke , Lawrence Blyn , Ray Ranken , Thomas A. Hall
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/6872 , C12Q1/70 , C12Q1/701 , C12Q2600/16 , G01N1/30 , G01N21/64 , G01N23/00 , G06F19/20
摘要： The present invention provides methods of: identifying pathogens in biological samples from humans and animals, resolving a plurality of etiologic agents present in samples obtained from humans and animals, determining detailed genetic information about such pathogens or etiologic agents, and rapid detection and identification of bioagents from environmental, clinical or other samples.
摘要翻译： 本发明提供了以下方法：鉴定来自人和动物的生物样品中的病原体，解决从人和动物获得的样品中存在的多种病原体，确定关于这种病原体或病原体的详细遗传信息，以及快速检测和鉴定生物代谢 来自环境，临床或其他样品。
-
公开(公告)号：US08046171B2
公开(公告)日：2011-10-25
申请号：US10418514
申请日：2003-04-18
申请人： David J. Ecker , Richard H. Griffey , Rangarajan Sampath , Steven A. Hofstadler , John McNeil , Stanley T. Crooke , Dino J. Sofianos , Karl H. Rudnick , Duane J. Knize , Roland B. Stoughton , Cecil L. Basham, Jr. , Clifford T. Lewis , Brons M. Larson , Jonathan R. Bar-on , Dennis P. Murray , David W. Robbins , John P. Penhune
发明人： David J. Ecker , Richard H. Griffey , Rangarajan Sampath , Steven A. Hofstadler , John McNeil , Stanley T. Crooke , Dino J. Sofianos , Karl H. Rudnick , Duane J. Knize , Roland B. Stoughton , Cecil L. Basham, Jr. , Clifford T. Lewis , Brons M. Larson , Jonathan R. Bar-on , Dennis P. Murray , David W. Robbins , John P. Penhune
CPC分类号： G06F19/22 , Y10T436/24
摘要： Rapid and definitive bioagent detection and identification can be carried out without nucleic acid sequencing. Analysis of a variety of bioagents and samples, such as air, fluid, and body samples, can be carried out to provide information useful for industrial, medical, and environmental purposes. Nucleic acid samples of unknown or suspected bioagents may be collected, optimal primer pairs may be selected, and the nucleic acid may be amplified. Expected mass spectra signal models may be generated and selected, the actual mass spectra of the amplicons may be obtained. The expected mass spectra most closely correlating with the actual mass spectra may be determined using a joint maximum likelihood analysis, and base counts for the actual mass spectra and the expected mass spectra may be obtained. The most likely candidate bioagents may then be determined.
摘要翻译： 可以在不进行核酸测序的情况下进行快速和确定的生物鉴定检测和鉴定。 可以对各种生物标本和样品（如空气，液体和身体样品）进行分析，以提供有用的工业，医疗和环境用途的信息。 可以收集未知或疑似生物标记的核酸样品，可以选择最佳引物对，并且可以扩增核酸。 可以生成和选择预期的质谱信号模型，可以获得扩增子的实际质谱。 可以使用联合最大似然分析来确定与实际质谱最密切相关的预期质谱，并且可以获得实际质谱和预期质谱的碱值。 然后可能确定最可能的候选生物标签。
-
-
-
-
-
-
-
-
-
搜索结果
151 条记录 (耗时 0.097 秒)
