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    BUFFERS FOR THE STABLE STORAGE OF NUCLEIC ACIDS
    2.
    发明申请
    BUFFERS FOR THE STABLE STORAGE OF NUCLEIC ACIDS 有权
    稳定存储核酸的缓冲剂

    公开(公告)号：US20120171685A1

    公开(公告)日：2012-07-05

    申请号：US13340976

    申请日：2011-12-30

    申请人： Mark W. Eshoo ,  David D. Duncan

    发明人： Mark W. Eshoo ,  David D. Duncan

    IPC分类号： C12Q1/68 ,  B65D85/00 ,  C09K15/32 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6806 ,  C09K15/30 ,  C12Q2527/119 ,  C12Q2527/125 ,  C12Q2527/137

    摘要： Provided herein are buffers for the stabilization of nucleic acid molecules. The buffers find particular use for the stabilization of trace amounts of nucleic acid molecules in a variety of environments, including repeated freeze/thaw cycles. For example, in some embodiments, provided herein are compositions comprising tris(hydroxymethyl)aminomethane (Tris), ethylenediaminetetraacetic acid (EDTA), polyadenylic acid, and a synthetic DNA oligonucleotide.

    摘要翻译： 本文提供了用于稳定核酸分子的缓冲液。 缓冲液特别用于在各种环境中稳定微量的核酸分子，包括反复的冻融循环。 例如，在一些实施方案中，本文提供了包含三（羟甲基）氨基甲烷（Tris），乙二胺四乙酸（EDTA），聚腺苷酸和合成DNA寡核苷酸的组合物。

    PARASITE DETECTION VIA ENDOSYMBIONT DETECTION
    4.
    发明申请
    PARASITE DETECTION VIA ENDOSYMBIONT DETECTION 有权
    通过内部检测进行PARASITE检测

    公开(公告)号：US20110223599A1

    公开(公告)日：2011-09-15

    申请号：US13046186

    申请日：2011-03-11

    申请人： Mark W. Eshoo ,  Christopher Crowder

    发明人： Mark W. Eshoo ,  Christopher Crowder

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12M1/34 ,  C12Q1/02

    CPC分类号： C12Q1/6893 ,  C12Q2600/156

    摘要： The present invention provides systems, methods, and compositions for identifying a subject as infected with a parasite by detecting nucleic acid from an endosymbiont of the parasite in a sample from the subject. In certain embodiments, the parasite is a nematode that infects humans or dogs (e.g., D. immitis, O. volvulus, W. bancrofti, B. timori, or B. malayi) and the endosymbiont is Wolbachia.

    摘要翻译： 本发明提供用于通过从受试者的样品中检测来自寄生虫的内共生体的核酸来鉴定被寄生虫感染的受试者的系统，方法和组合物。 在某些实施方案中，寄生虫是感染人或狗的线虫（例如，D.emmitis，O.volvulus，W.bancrofti，B.trorori或B.Marayi），并且内共生体是Wolbachia。

    METHODS AND COMPOSITIONS FOR MULTIPLE DISPLACEMENT AMPLIFICATION OF NUCLEIC ACIDS
    5.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLE DISPLACEMENT AMPLIFICATION OF NUCLEIC ACIDS 有权
    用于多重位移放大核酸的方法和组合物

    公开(公告)号：US20100240102A1

    公开(公告)日：2010-09-23

    申请号：US12602641

    申请日：2008-05-30

    申请人： David A. Duncan ,  Mark W. Eshoo

    发明人： David A. Duncan ,  Mark W. Eshoo

    IPC分类号： C12P19/34 ,  C07H21/04 ,  C12N9/00 ,  C12N9/14

    CPC分类号： C12Q1/6846 ,  C12Q2531/119 ,  C12Q2527/125 ,  C12Q2525/101

    摘要： Disclosed are methods for multiple displacement amplification of a nucleic acid sequence in a sample. The nucleic acid is contacted with a reaction mixture that includes a set of oligonucleotide primers and a plurality of polymerase enzymes. The reaction mixture is subjected to conditions under which the nucleic acid sequence is amplified to produce an amplification product in a multiple displacement amplification reaction. Also disclosed are kits containing a set of oligonucleotide primers with random sequences having lengths of 6 to 8 nucleobases. At least some of the individual members of the primers have one or more ribose modifications that stabilize or lock the ribose ring in a 3′ -endo conformation. At least some of the primers have one or more universal nucleobases.

    摘要翻译： 公开了用于样品中核酸序列的多重位移扩增的方法。 核酸与包含一组寡核苷酸引物和多个聚合酶的反应混合物接触。 使反应混合物经受在多位置扩增反应中扩增核酸序列产生扩增产物的条件。 还公开了包含一组具有长度为6至8个核碱基的随机序列的寡核苷酸引物的试剂盒。 引物的至少一些单独成员具有一个或多个核糖修饰，其稳定或锁定3'内质网构型中的核糖环。 至少一些引物具有一个或多个通用核碱基。

    Multiple displacement amplification
    8.
    发明授权
    Multiple displacement amplification 有权

    公开(公告)号：US09890408B2

    公开(公告)日：2018-02-13

    申请号：US12905819

    申请日：2010-10-15

    申请人： Mark W. Eshoo ,  John Picuri ,  Curtis Phillipson

    发明人： Mark W. Eshoo ,  John Picuri ,  Curtis Phillipson

    IPC分类号： C12Q1/64 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12P19/34 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2531/119 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2527/127 ,  C12Q2531/113

    摘要： The present invention provides methods kits and systems for performing multiple displacement amplification reactions. In one method a sample of nucleic acid is provided. The nucleic acid is contacted with a reaction mixture which includes a set of oligonucleotide primers, a one or more polymerase enzymes and a detergent. The reaction mixture is then subjected to conditions under which the nucleic acid sequence is amplified to produce an amplified product in a multiple displacement reaction. The method may also be carried out by contacting the nucleic acid with the reaction mixture in the form of an emulsion. A kit is also provided for carrying out either the methods described above. The kit includes one or more polymerases, a plurality of primers and a detergent. The kit may also include a hydrophobic polymer and may include instructions for performing a multiple displacement amplification reaction on a nucleic acid sample.