公开(公告)号：US20170275381A1

公开(公告)日：2017-09-28

申请号：US15465439

申请日：2017-03-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Dieter Heindl ,  Christian Klein ,  Alfred Mertens ,  Volker Schmid ,  Michael Schraemi ,  Monika Soukupova ,  Michael Tacke

IPC: C07K16/46 ,  G01N33/531

CPC classification number: C07K16/468 ,  G01N33/531

Abstract: A bivalent binding agent having a first monovalent binder that binds to a polypeptide epitope of a target polypeptide, a second monovalent binder that binds to a posttranslational polypeptide modification on the target polypeptide and a linker. Further disclosed are methods for the detection of a posttranslationally modified target polypeptide, for making the disclosed bivalent binding agent, and for use of the disclosed bivalent binding agent in histological staining procedures.

公开(公告)号：US20170096487A1

公开(公告)日：2017-04-06

申请号：US15083686

申请日：2016-03-29

Applicant: Roche Diagnostics Operations, Inc. ,  Ventana Medical Systems, Inc.

Inventor： Monika Soukupova ,  Michael Schraeml ,  Birgit Bossenmaier ,  Patrick C. Roche ,  Michael Gerg ,  Sebastian Dziadek

IPC: C07K16/28 ,  G01N33/574 ,  C07K16/30 ,  G01N33/74 ,  G01N1/30

CPC classification number: C07K16/2863 ,  C07K16/3023 ,  C07K16/3046 ,  C07K16/32 ,  C07K2317/33 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  C07K2317/94 ,  G01N1/30 ,  G01N33/57492 ,  G01N33/74 ,  G01N2001/302 ,  G01N2333/71

Abstract: The present invention relates to isolated antibodies, or an antigen portions thereof, which bind to human HER3. The novel antibodies are of great utility since they allow for the sensitive and specific detection of human HER3. Detection of human HER3 is, e.g., possible in a tissue sample, even when such tissue sample is a formalin-fixed paraffin embedded tissue (FFPET) sample.

公开(公告)号：US20160137728A1

公开(公告)日：2016-05-19

申请号：US15003988

申请日：2016-01-22

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Herbert Andres ,  Hartmut Duefel ,  Michael Gerg ,  Frank Kowalewsky ,  Christian Scholz ,  Michael Schraeml

IPC: C07K16/22

CPC classification number: C07K16/22 ,  C07K14/475 ,  C07K14/65 ,  C07K16/1203 ,  C07K16/40 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/23 ,  C07K2319/24 ,  C07K2319/35 ,  C07K2319/41 ,  C07K2319/43 ,  C12N9/90 ,  C12Y502/01008 ,  G01N33/573 ,  G01N33/74 ,  G01N2333/99

Abstract: Isolated antibodies that specifically bind to an epitope comprised in the stretch of amino acids ranging from amino acid 76 to amino acid 84 of human insulin-like growth factor-1 precursor (SEQ ID NO:1). Use of the novel antibodies for the sensitive and specific detection of insulin-like growth factor-1, in some embodiments while in the presence of high excess concentration of insulin-like growth factor-2, for example in a bodily fluid sample.

Abstract translation: 分离的抗体特异性结合包含在人类胰岛素样生长因子-1前体的氨基酸76至氨基酸84的氨基酸区段中的表位（SEQ ID NO：1）。 在一些实施方案中，当存在高过量浓度的胰岛素样生长因子-2（例如在体液样品中）时，使用新型抗体用于敏感和特异性检测胰岛素样生长因子-1。

公开(公告)号：US20190324025A1

公开(公告)日：2019-10-24

申请号：US16450181

申请日：2019-06-24

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Dieter Heindl ,  Lars Hillringhaus ,  Klaus Hirzel ,  Caroline Dorothea Hojer ,  Florian Huber ,  Hans-Peter Josel ,  Thomas Meier ,  Michael Schraeml ,  Edgar Voss

IPC: G01N33/543 ,  C07K16/44

Abstract: The present invention relates to a monoclonal antibody capable of binding to biotin. In one embodiment the monoclonal antibody according to the invention also does not bind to a biotin moiety on a biotinylated molecule, wherein the biotin moiety is attached to the molecule via the carbon atom of the carboxyl function of the valeric acid moiety of biotin. Also disclosed is a method for generation of an antibody as disclosed herein. The monoclonal antibody according to the invention is of specific use in a method for measuring an analyte in a sample, wherein a (strept)avidin/biotin pair is used to bind a biotinylated analyte specific binding agent to a (strept)avidin coated solid phase.

公开(公告)号：US20170269068A1

公开(公告)日：2017-09-21

申请号：US15614856

申请日：2017-06-06

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Elke Faatz ,  Michael Gerg ,  Hans-Peter Josel ,  Christian Vogl

IPC: G01N33/537 ,  G01N33/82 ,  C07K16/26 ,  G01N33/541

CPC classification number: G01N33/537 ,  C07K16/26 ,  G01N33/541 ,  G01N33/82

Abstract: The present invention concerns an in vitro method for measurement of 25-hydroxyvitamin D, wherein the potentially interfering compound 24,25-dihydroxyvitamin D3 is blocked by a binding agent specifically binding to 24,25-dihydroxyvitamin D3 and not binding to 25-hydroxyvitamin D.

公开(公告)号：US08658109B2

公开(公告)日：2014-02-25

申请号：US13766297

申请日：2013-02-13

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Michael Gerg ,  Rupert Hermann ,  Rosa Isabel Saez Diaz

IPC: B01L3/00 ,  C12Q1/68 ,  C12P19/34

CPC classification number: G01N1/30

Abstract: The present disclosure relates to novel bis-maleic anhydrides and to the surprising discovery that bis-maleic anhydride cross-linking agents can be used for preservation/fixation of a cell or tissue sample. Various bis-maleic anhydride cross-linking agent scan be used in methods requiring fixation of a cell or tissue sample. These reagents and methods are especially useful in procedures that require that the fixation agent be removed in order to facilitate analysis with other reagents. The inventive reagents and methods make it easier to reliably assay for various proteins, a nucleic acid and the like using analytical methods such as like immunohistochemistry, fluorescence in situ hybridization, RT-PCR, and the like.

Abstract translation: 本公开涉及新颖的双马来酸酐，以及令人惊奇的发现，即双马来酸酐交联剂可用于保存/固定细胞或组织样品。 各种双马来酸酐交联剂扫描用于需要固定细胞或组织样品的方法。 这些试剂和方法在需要除去固定剂以便于与其它试剂分析的方法中特别有用。 本发明的试剂和方法使得可以使用诸如免疫组织化学，荧光原位杂交，RT-PCR等的分析方法更容易地可靠地测定各种蛋白质，核酸等。

公开(公告)号：US20130288267A1

公开(公告)日：2013-10-31

申请号：US13923646

申请日：2013-06-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Dieter Heindl ,  Alfred Mertens ,  Christoph Rutz ,  Michael Schraeml ,  Monika Soukupova ,  Claudio Sustmann ,  Michael Tacke ,  Jan van Dieck

IPC: C07K16/46

CPC classification number: C07K16/468 ,  C07K16/18 ,  C07K16/2863 ,  C07K16/32 ,  C07K16/44 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/92 ,  C07K2317/94 ,  G01N33/6857

Abstract: A bivalent binding agent, capable of binding a polypeptide dimer, consisting of two monovalent binders linked to each other via a linker, the first monovalent binder binds an epitope of a first target polypeptide comprised in said dimer and the second monovalent binder binds to an epitope of a second target polypeptide comprised in said dimer. Each monovalent binder has a Kdiss in the range of 5×10−3/sec to 10−4/sec, and the bivalent binding agent has a Kdiss of 3×10−5/sec or less. Methods of making and using such bivalent binding agent in histological staining procedures are also disclosed.

Abstract translation: 能够结合多肽二聚体的二价结合剂，其由通过接头彼此连接的两个单价结合物组成，所述第一单价键合物结合包含在所述二聚体中的第一目标多肽的表位，并且所述第二单价键合物结合表位 的包含在所述二聚体中的第二目标多肽。 每个一价粘合剂的Kdiss在5×10-3 /秒至10-4 /秒的范围内，二价结合剂的Kdiss为3×10-5 /秒或更小。 还公开了在组织学染色方法中制备和使用这种二价结合剂的方法。