公开(公告)号：US20130323823A1

公开(公告)日：2013-12-05

申请号：US13941353

申请日：2013-07-12

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Risha Bond ,  Walter Rakitsky ,  Alejandro Marangoni ,  Diza Braksmayer

IPC: C12P7/64

CPC classification number: C12P7/6409 ,  A23D9/00 ,  A23D9/013 ,  C07C57/03 ,  C07J9/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/113 ,  C12N15/8247 ,  C12N2310/531 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12P7/6472 ,  C12P33/00 ,  C12R1/89 ,  C12Y203/01015 ,  C12Y203/0102 ,  C12Y203/01041 ,  C12Y203/01051 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y302/01014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52

Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

公开(公告)号：US09249441B2

公开(公告)日：2016-02-02

申请号：US14474244

申请日：2014-09-01

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Risha Bond ,  Walter Rakitsky ,  Alejandro Marangoni ,  Diza Braksmayer

IPC: C12N1/20 ,  C12P33/00 ,  C12P7/64 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  A23D9/00 ,  C07C57/03 ,  C07J9/00 ,  C12N15/82 ,  A23D9/013 ,  C11B1/00 ,  C12R1/89

CPC classification number: C12P7/6409 ,  A23D9/00 ,  A23D9/013 ,  C07C57/03 ,  C07J9/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/113 ,  C12N15/8247 ,  C12N2310/531 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12P7/6472 ,  C12P33/00 ,  C12R1/89 ,  C12Y203/01015 ,  C12Y203/0102 ,  C12Y203/01041 ,  C12Y203/01051 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y302/01014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52

Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

公开(公告)号：US20160010066A1

公开(公告)日：2016-01-14

申请号：US14796406

申请日：2015-07-10

Applicant: Solazyme, Inc.

Inventor： David Davis ,  George Rudenko ,  Aravind Somanchi ,  Jason Casolari ,  Scott Franklin ,  Aren Ewing

IPC: C12N9/10 ,  C12P7/64

CPC classification number: C12N9/1029 ,  C12N15/82 ,  C12P7/64 ,  C12P7/6463 ,  C12P33/00 ,  C12Y203/01041

Abstract: The present invention relates to beta-ketoacyl ACP synthase genes of the KASI/KASIV type and proteins encoded by these genes. The genes can be included in nucleic acid constructs, vectors or host cells. Expression of the gene products can alter the fatty acid profile of host cells. The KAS genes can be combined with a FATA or FATB thioesterase gene to create a cell that produces an increased amount of C8-C16 fatty acids. Suitable host cells include plastidic cells of plants or microalgae. Oleaginous microalga host cells with the new genes are disclosed.

Abstract translation: 本发明涉及KASI / KASIV型的β-酮酸酰基ACP合酶基因和由这些基因编码的蛋白质。 这些基因可以包含在核酸构建体，载体或宿主细胞中。 基因产物的表达可以改变宿主细胞的脂肪酸分布。 可以将KAS基因与FATA或FATB硫酯酶基因组合以产生产生增加量的C 8 -C 16脂肪酸的细胞。 合适的宿主细胞包括植物或微藻的质体细胞。 公开了具有新基因的油性微藻宿主细胞。

公开(公告)号：US09200307B2

公开(公告)日：2015-12-01

申请号：US13941342

申请日：2013-07-12

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Risha Bond ,  Walter Rakitsky ,  Alejandro Marangoni ,  Diza Braksmayer

IPC: C12P7/64 ,  C12N9/88 ,  C12P33/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  A23D9/00 ,  C07C57/03 ,  C07J9/00 ,  C12N15/82 ,  A23D9/013 ,  C11B1/00 ,  C12R1/89

CPC classification number: C12P7/6409 ,  A23D9/00 ,  A23D9/013 ,  C07C57/03 ,  C07J9/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/113 ,  C12N15/8247 ,  C12N2310/531 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12P7/6472 ,  C12P33/00 ,  C12R1/89 ,  C12Y203/01015 ,  C12Y203/0102 ,  C12Y203/01041 ,  C12Y203/01051 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y302/01014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52

Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsatturated-saturated type.

Abstract translation: 重组DNA技术用于产生产生具有所需脂肪酸谱和区域特异性或立体特异性谱的甘油三酯油的含油重组细胞。 基因操作包括编码硬脂酰-ACP脱氧酶，δ12脂肪酸去饱和酶，酰基-ACP硫酯酶，酮酰基-ACP合成酶和溶血磷脂酰基转移酶的那些。 所生产的油可以具有增强的氧化或热稳定性，或可用作煎炸油，起酥油，卷入起酥油，回火脂肪，可可脂替代物，润滑剂或用作各种化学方法的原料。 脂肪酸分布可以富集于中链轮廓，或者油可以富集饱和不饱和饱和型甘油三酸酯。

公开(公告)号：US09102973B2

公开(公告)日：2015-08-11

申请号：US13941357

申请日：2013-07-12

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Risha Bond ,  Walter Rakitsky ,  Alejandro Marangoni ,  Diza Braksmayer

IPC: C12P7/64 ,  C12N1/11 ,  C12R1/89 ,  C12P33/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  A23D9/00 ,  C07C57/03 ,  C07J9/00 ,  C12N15/82

CPC classification number: C12P7/6409 ,  A23D9/00 ,  A23D9/013 ,  C07C57/03 ,  C07J9/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/113 ,  C12N15/8247 ,  C12N2310/531 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12P7/6472 ,  C12P33/00 ,  C12R1/89 ,  C12Y203/01015 ,  C12Y203/0102 ,  C12Y203/01041 ,  C12Y203/01051 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y302/01014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52

Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

公开(公告)号：US20130338385A1

公开(公告)日：2013-12-19

申请号：US13804185

申请日：2013-03-14

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Risha Bond ,  Walter Rakitsky ,  Alejandro Marangoni

IPC: C12P7/64

CPC classification number: C12P7/64 ,  A23D9/02 ,  C10L1/026 ,  C11B11/00 ,  C12N9/0071 ,  C12N9/2431 ,  C12P7/6463 ,  C12P7/649 ,  C12Y114/19002 ,  C12Y301/03002 ,  Y02E50/13 ,  Y02T50/678

Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

Abstract translation: 重组DNA技术用于产生产生具有所需脂肪酸谱和区域特异性或立体特异性谱的甘油三酯油的含油重组细胞。 基因操作包括编码硬脂酰-ACP脱氧酶，δ12脂肪酸去饱和酶，酰基-ACP硫酯酶，酮酰基-ACP合成酶和溶血磷脂酰基转移酶的那些。 所生产的油可以具有增强的氧化或热稳定性，可用作煎炸油，起酥油，卷入起酥油，回火脂肪，可可脂替代物，润滑剂或作为各种化学方法的原料。 脂肪酸分布可以富集中链型，或者油可富含饱和不饱和饱和型甘油三酸酯。

公开(公告)号：US20130331584A1

公开(公告)日：2013-12-12

申请号：US13941346

申请日：2013-07-12

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Risha Bond ,  Walter Rakitsky ,  Alejandro Marangoni ,  Diza Braksmayer

IPC: C12P33/00

CPC classification number: C12P7/6409 ,  A23D9/00 ,  A23D9/013 ,  C07C57/03 ,  C07J9/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/113 ,  C12N15/8247 ,  C12N2310/531 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12P7/6472 ,  C12P33/00 ,  C12R1/89 ,  C12Y203/01015 ,  C12Y203/0102 ,  C12Y203/01041 ,  C12Y203/01051 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y302/01014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52

Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

公开(公告)号：US20130323382A1

公开(公告)日：2013-12-05

申请号：US13941342

申请日：2013-07-12

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Risha Bond ,  Walter Rakitsky ,  Alejandro Marangoni ,  Diza Braksmayer

IPC: C12P7/64 ,  C07J9/00 ,  A23D9/00 ,  C07C57/03

CPC classification number: C12P7/6409 ,  A23D9/00 ,  A23D9/013 ,  C07C57/03 ,  C07J9/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/113 ,  C12N15/8247 ,  C12N2310/531 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12P7/6472 ,  C12P33/00 ,  C12R1/89 ,  C12Y203/01015 ,  C12Y203/0102 ,  C12Y203/01041 ,  C12Y203/01051 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y302/01014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52

Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsatturated-saturated type.

Abstract translation: 重组DNA技术用于产生产生具有所需脂肪酸谱和区域特异性或立体特异性谱的甘油三酯油的含油重组细胞。 基因操作包括编码硬脂酰-ACP脱氧酶，δ12脂肪酸去饱和酶，酰基-ACP硫酯酶，酮酰基-ACP合成酶和溶血磷脂酰基转移酶的那些。 所生产的油可以具有增强的氧化或热稳定性，或可用作煎炸油，起酥油，卷入起酥油，回火脂肪，可可脂替代物，润滑剂或用作各种化学方法的原料。 脂肪酸分布可以富集于中链轮廓，或者油可以富集饱和不饱和饱和型甘油三酸酯。

公开(公告)号：US20130316410A1

公开(公告)日：2013-11-28

申请号：US13865974

申请日：2013-04-18

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Risha Bond ,  Walter Rakitsky ,  Alejandro Marangoni ,  Diza Braksmayer

IPC: C12P7/64

CPC classification number: C12P7/6409 ,  A23D9/00 ,  A23D9/013 ,  C07C57/03 ,  C07J9/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/113 ,  C12N15/8247 ,  C12N2310/531 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12P7/6472 ,  C12P33/00 ,  C12R1/89 ,  C12Y203/01015 ,  C12Y203/0102 ,  C12Y203/01041 ,  C12Y203/01051 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y302/01014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52

Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

Abstract translation: 重组DNA技术用于产生产生具有所需脂肪酸谱和区域特异性或立体特异性谱的甘油三酯油的含油重组细胞。 基因操作包括编码硬脂酰-ACP脱氧酶，δ12脂肪酸去饱和酶，酰基-ACP硫酯酶，酮酰基-ACP合成酶和溶血磷脂酰基转移酶的那些。 所生产的油可以具有增强的氧化或热稳定性，或可用作煎炸油，起酥油，卷入起酥油，回火脂肪，可可脂替代物，润滑剂或用作各种化学方法的原料。 脂肪酸分布可以富集中链型，或者油可富含饱和不饱和饱和型甘油三酸酯。