公开(公告)号：US08980646B2

公开(公告)日：2015-03-17

申请号：US10581294

申请日：2004-03-26

Applicant: Sudhir Paul ,  Yasuhiro Nishiyama

Inventor： Sudhir Paul ,  Yasuhiro Nishiyama

IPC: C07K16/00 ,  C12P21/08 ,  C07K16/28 ,  C07K16/10 ,  C07K16/44 ,  C12Q1/37 ,  G01N33/68

CPC classification number: C07K16/2863 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/01 ,  C07K16/1063 ,  C07K16/44 ,  C07K2317/21 ,  C07K2317/34 ,  C12Q1/37 ,  G01N33/6854 ,  G01N2333/901

Abstract: Improved methods for the production, selection and inhibition of catalytic and covalent antibodies are disclosed.

Abstract translation: 公开了用于生产，选择和抑制催化和共价抗体的改进方法。

公开(公告)号：US20110287031A1

公开(公告)日：2011-11-24

申请号：US12387020

申请日：2009-04-27

Applicant: Sudhir Paul ,  Yasuhiro Nishiyama

Inventor： Sudhir Paul ,  Yasuhiro Nishiyama

IPC: A61K39/395 ,  C07K7/08 ,  C07K19/00 ,  A61K38/08 ,  C12N9/00 ,  C12N9/96 ,  C07F9/6561 ,  A61P35/00 ,  A61P35/02 ,  A61P19/02 ,  A61P11/06 ,  A61P27/02 ,  A61P19/04 ,  A61P37/04 ,  C07K7/06

CPC classification number: A61M25/0905 ,  A61M2025/09108

Abstract: Improved methods for the production, selection and inhibition of catalytic antibodies are disclosed.

Abstract translation: 公开了生产，选择和抑制催化抗体的改进方法。

公开(公告)号：US07524663B2

公开(公告)日：2009-04-28

申请号：US10930548

申请日：2004-08-31

Applicant: Sudhir Paul ,  Yasuhiro Nishiyama

Inventor： Sudhir Paul ,  Yasuhiro Nishiyama

IPC: C12N9/00 ,  C07K1/00 ,  A61K39/395 ,  C07K14/47

CPC classification number: C12N9/0002 ,  A61K2039/505 ,  C07K16/1063 ,  C07K16/32 ,  C07K2319/00

Abstract: Improved methods for the production, selection and inhibition of catalytic antibodies are disclosed.

Abstract translation: 公开了生产，选择和抑制催化抗体的改进方法。

公开(公告)号：US20070179083A1

公开(公告)日：2007-08-02

申请号：US10581296

申请日：2004-03-26

Applicant: Sudhir Paul ,  Yasuhiro Nishiyama

Inventor： Sudhir Paul ,  Yasuhiro Nishiyama

IPC: A61K38/16 ,  A61K48/00 ,  C07H21/02

CPC classification number: A61K47/48238 ,  A61K47/62 ,  A61K2039/505 ,  A61K2039/60 ,  A61K2039/6093 ,  C07K14/005 ,  C07K16/1063 ,  C07K2317/34 ,  C07K2317/76 ,  C12N2740/16122

Abstract: A covalently reactive ligand analogue (CAL) of formula (1): wherein, L1 . . . Lx . . . Lm are components defining a ligand determinant, Lx is a component unit of the ligand determinant selected from the group consisting of an amino acid residue, sugar residue, a fatty acid residue and a nucleotide, L′ is a functional group of Lx, Y″ is atom, covalent bond or linker, Y′ is an optional charged or neutral group Y is a covalently reactive electrophilic group that reacts specifically with a receptor that binds to said ligand determinant, and n is an integer from 1 to 1000 m is an integer from 1 to 30.

Abstract translation: 式（1）的共价反应性配体类似物（CAL）：其中，L 1。 。 。 L＆lt; x＆gt;。 。 。 L>>>>>> acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid acid 残基和核苷酸，L'是L x X的官能团，Y“是原子，共价键或连接基，Y'是任选的带电荷或中性基团Y是共价反应的亲电基团， 与与所述配体决定簇结合的受体特异性地反应，n为1〜1000μm的整数，为1〜30的整数。

公开(公告)号：US20070105092A1

公开(公告)日：2007-05-10

申请号：US10581294

申请日：2004-03-26

Applicant: Sudhir Paul ,  Yasuhiro Nishiyama

Inventor： Sudhir Paul ,  Yasuhiro Nishiyama

IPC: A01K67/027 ,  C12Q1/70 ,  C12P21/00 ,  G01N33/53 ,  G01N33/574 ,  C12N9/00 ,  C12N5/06 ,  C12P21/04

CPC classification number: C07K16/2863 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/01 ,  C07K16/1063 ,  C07K16/44 ,  C07K2317/21 ,  C07K2317/34 ,  C12Q1/37 ,  G01N33/6854 ,  G01N2333/901

Abstract: Improved methods for the production, selection and inhibition of catalytic and covalent antibodies are disclosed.

Abstract translation: 公开了用于生产，选择和抑制催化和共价抗体的改进方法。

公开(公告)号：US20050214295A1

公开(公告)日：2005-09-29

申请号：US11058728

申请日：2005-02-15

Applicant: Sudhir Paul ,  Gennady Gololobov ,  Larry Smith

Inventor： Sudhir Paul ,  Gennady Gololobov ,  Larry Smith

IPC: A61K39/00 ,  A61K38/00 ,  A61K39/395 ,  A61P5/14 ,  A61P11/06 ,  A61P17/00 ,  A61P19/02 ,  A61P19/04 ,  A61P29/00 ,  A61P35/00 ,  A61P37/06 ,  C07K7/04 ,  C07K16/00 ,  C07K16/10 ,  C07K16/32 ,  C12N9/00 ,  C12N9/64

CPC classification number: C07K16/1063 ,  A61K2039/505 ,  C07K16/32 ,  C07K2317/34 ,  C12N9/0002

Abstract: Covalently reactive antigen analogs are disclosed herein. The antigens of the invention may be used to stimulate production of catalytic antibodies specific for predetermined antigens assocated with particular medical disorders. The antigen analogs may also be used to permanently inactivate endogenously produced catalytic antibodies produced in certain autoimmune diseases as well as in certain lymphoproliferative disorders.

Abstract translation: 本文公开了共价反应性抗原类似物。 本发明的抗原可用于刺激与特定医学病症有关的预定抗原特异性的催化抗体的产生。 抗原类似物也可用于永久灭活在某些自身免疫性疾病以及某些淋巴组织增生性疾病中产生的内源性产生的催化抗体。

公开(公告)号：US5770570A

公开(公告)日：1998-06-23

申请号：US519180

申请日：1995-08-25

Applicant: Sudhir Paul ,  Yasuko Noda ,  Israel Rubinstein

Inventor： Sudhir Paul ,  Yasuko Noda ,  Israel Rubinstein

IPC: A61K9/127 ,  A61K38/00 ,  A61K38/22 ,  A61K47/42 ,  A61P1/00 ,  A61P1/04 ,  A61P3/00 ,  A61P7/06 ,  A61P9/12 ,  A61P11/00 ,  A61P25/18 ,  A61P43/00 ,  A61K38/17 ,  A61K38/25

CPC classification number: A61K9/127 ,  A61K38/2278

Abstract: Provided is a method for delivering a vasoactive intestinal polypeptide (VIP) liposome product to a target tissue of a mammal. The VIP is expressed on and in a liposome. Also provided is a method of preparing the VIP liposome product comprising cholesterol, phosphatidyl choline, and phosphatidyl glycerol.

Abstract translation: 提供了一种将血管活性肠肽（VIP）脂质体产物递送至哺乳动物的靶组织的方法。 VIP在脂质体中和脂质体中表达。 还提供了制备包含胆固醇，磷脂酰胆碱和磷脂酰甘油的VIP脂质体产物的方法。

公开(公告)号：US5599538A

公开(公告)日：1997-02-04

申请号：US274306

申请日：1994-07-12

Applicant: Sudhir Paul ,  Lan Li ,  Srini Kaveri

Inventor： Sudhir Paul ,  Lan Li ,  Srini Kaveri

IPC: C07K14/575 ,  C07K16/06 ,  C07K16/26 ,  C12N9/00 ,  C12N9/48 ,  A61K39/395

CPC classification number: C12N9/0002 ,  C07K14/57563 ,  C07K16/06 ,  C07K16/26 ,  C12N9/48 ,  C07K2317/34

Abstract: Autoantibodies which enhance the rate of a chemical reaction of a substrate, processes for their preparation, their use and compositions thereof are disclosed. In particular, an autoantibody capable of catalyzing the hydrolysis of the peptide bond between amino acid residues 16 and 17 in the neurotransmitter vasoactive intestinal peptide (VIP) is disclosed. Human anti-thyroglobulin antibodies isolated by chromatography on protein-A and immobilized Tg hydrolyzed radiolabeled Tg, as shown by generation of several lower-sized products on SDS-electrophoresis gels. The activity displayed a K.sub.m value of a 39 nM property typical of an antibody-combining site. Tg-antibodies also hydrolyzed commercially available peptidyl-methylcoumarinamide (MCA) substrates, displaying a preference for arg-MCA and lys-MCA containing conjugates. The hydrolysis of pro-phe-arg-MCA was characterized by K.sub.m (17 .mu.M) and k.sub.cat 0.06 min.sup.-1. Peptidyl-MCA hydrolysis was inhibited potently by thyroglobulin (K.sub.i 24 nM), suggesting a catalytic site/located in the antibody combining site. In control experiments, the hydrolytic activities were removed by immunoadsorption with immobilized anti-human IgG, and IgG depleted of the Tg-specific antibodies by affinity chromatography did not display Tg and pro-phe-arg-MCA hydrolyzing activities.

Abstract translation: 公开了增加底物的化学反应速率的自身抗体，其制备方法，用途及其组合物。 特别地，公开了能够催化神经递质血管活性肠肽（VIP）中的氨基酸残基16和17之间的肽键的水解的自身抗体。 通过蛋白质-A上的色谱法分离的人类抗甲状腺球蛋白抗体和固定的Tg水解的放射性标记的Tg，如SDS-电泳凝胶上几种较低产品的产生所示。 该活性显示抗体结合位点典型的39nM性质的Km值。 Tg抗体还水解可商购的肽基 - 甲基香豆素酰胺（MCA）底物，显示出对arg-MCA和含有lysA-MCA的缀合物的偏好。 原位杂交MCA的水解表征为Km（17μM）和kcat0.06min-1。 肽基 - MCA水解被甲状腺球蛋白（Ki 24 nM）有效抑制，表明位于抗体结合位点的催化位点。 在对照实验中，通过用固定的抗人IgG进行免疫吸附除去水解活性，通过亲和层析除去Tg-特异性抗体的IgG不显示Tg和亲和-MACA水解活性。