公开(公告)号：US20090218236A1

公开(公告)日：2009-09-03

申请号：US12413214

申请日：2009-03-27

申请人： Hong Xie ,  Zhiqiang Gao

发明人： Hong Xie ,  Zhiqiang Gao

IPC分类号： G01N27/26 ,  C07F15/06 ,  C12N11/02 ,  C07F15/02 ,  C07F15/04 ,  C07F1/00 ,  C07F1/08 ,  C07F1/10 ,  C07F1/12 ,  C07F3/02 ,  C07F3/06

CPC分类号： C07F15/0026

摘要： Compositions and methods for electrochemical detection of an analyte comprising a transition metal compound wherein M is a metallic element that can form a coordinate bond to nitrogen; R and R′ are coordinated to M at their nitrogen atoms; L is a linking ligand; Z is chlorine or bromine; m can be from 1 to 6 and X is an anion, or combination of anions, that balances the charge m. Also provided are electrochemical tags and methods of detection.

摘要翻译： 用于电化学检测包含过渡金属化合物的分析物的组合物和方法，其中M是能够形成与氮的配位键的金属元素; R和R'在氮原子上与M配位; L是连接配体; Z是氯或溴; m可以是1至6，X是阴离子或阴离子的组合，其平衡电荷m。 还提供了电化学标签和检测方法。

公开(公告)号：US07479557B2

公开(公告)日：2009-01-20

申请号：US10866370

申请日：2004-06-10

申请人： Zhiqiang Gao ,  Fang Xie

发明人： Zhiqiang Gao ,  Fang Xie

IPC分类号： C07F15/00 ,  C07D413/14 ,  C12Q1/68

CPC分类号： C07F15/0026

摘要： The invention is directed to a compound having the general formula (I): wherein each of La and Lb is an independently selected from a linking moiety comprising 0 to 10 main chain atoms, optionally substituted; each of Za and Zb is an independently selected complexing moiety comprising at least one nitrogen atom; either both or one of Za and/or Zb is coordinatively bonded to a respective transition metal complex MaVa and MbVb through said nitrogen atom, wherein each of Ma and Mb is an independently selected transition metal, and each of Va and Vb is an independently selected valence group.

摘要翻译： 本发明涉及具有通式（I）的化合物：其中La和Lb各自独立地选自包含0至10个主链原子的连接部分，任选被取代; Za和Zb中的每一个是包含至少一个氮原子的独立选择的络合部分; Za和/或Zb中的任一个或Zb通过所述氮原子与相应的过渡金属络合物MaVa和MbVb配位键合，其中Ma和Mb各自为独立选择的过渡金属，Va和Vb中的每一个为独立选择的 化合价。

公开(公告)号：US20080272006A1

公开(公告)日：2008-11-06

申请号：US12095498

申请日：2006-11-28

申请人： Zhiqiang Gao

发明人： Zhiqiang Gao

IPC分类号： G01N27/26

CPC分类号： G01N27/3277

摘要： There is presently provided an electrochemical method of detecting an analyte in a sample involving use of electroactive compound Ru(PD)2Cl2 as a label.

摘要翻译： 目前提供了一种用于检测涉及使用电活性化合物Ru（PD）2 Cl 2 2 N 2作为标记的样品中的分析物的电化学方法。

公开(公告)号：US08406905B2

公开(公告)日：2013-03-26

申请号：US13212274

申请日：2011-08-18

申请人： Zhiqiang Gao

发明人： Zhiqiang Gao

IPC分类号： G05B1/06 ,  G05B13/00 ,  G05B13/02

CPC分类号： G05B13/024 ,  G05B5/01 ,  G05B11/42 ,  G05B13/042 ,  G05B15/02 ,  G05B19/416 ,  G05B2219/33347

摘要： Controller scaling and parameterization are described. Techniques that can be improved by employing the scaling and parameterization include, but are not limited to, controller design, tuning and optimization. The scaling and parameterization methods described here apply to transfer function based controllers, including PID controllers. The parameterization methods also applies to state feedback and state observer based controllers, as well as linear active disturbance rejection controllers. It is emphasized that this abstract is provided to comply with the rules requiring an abstract that will allow a searcher or other reader to quickly ascertain the subject matter of the application. It is submitted with the understanding that it will not be used to interpret or limit the scope or meaning of the claims.

公开(公告)号：US08180464B2

公开(公告)日：2012-05-15

申请号：US12195353

申请日：2008-08-20

申请人： Zhiqiang Gao ,  Gang Tian

发明人： Zhiqiang Gao ,  Gang Tian

IPC分类号： G05B13/02

CPC分类号： G05B13/048 ,  G05B5/01 ,  G05B11/42 ,  G05B13/02 ,  G06F17/10

摘要： Multiple designs, systems, methods and processes for controlling a system or plant using an extended active disturbance rejection control (ADRC) based controller are presented. The extended ADRC controller accepts sensor information from the plant. The sensor information is used in conjunction with an extended state observer in combination with a predictor that estimates and predicts the current state of the plant and a co-joined estimate of the system disturbances and system dynamics. The extended state observer estimates and predictions are used in conjunction with a control law that generates an input to the system based in part on the extended state observer estimates and predictions as well as a desired trajectory for the plant to follow.

摘要翻译： 提出了使用基于扩展主动干扰抑制控制（ADRC）的控制器来控制系统或设备的多种设计，系统，方法和过程。 扩展的ADRC控制器接收来自工厂的传感器信息。 传感器信息与扩展状态观察器结合使用，预测器与估计和预测设备的当前状态以及系统干扰和系统动态的共同估计结合使用。 扩展状态观察者估计和预测结合使用一部分基于扩展状态观察者估计和预测以及工厂遵循的期望轨迹而产生系统输入的控制规则。

公开(公告)号：US07902362B2

公开(公告)日：2011-03-08

申请号：US12317359

申请日：2008-12-22

申请人： Zhiqiang Gao ,  Fang Xie

发明人： Zhiqiang Gao ,  Fang Xie

IPC分类号： C07F15/00 ,  C12Q1/68

CPC分类号： C07F15/0026

摘要： The invention is directed to a compound having the general formula (1): wherein each of La and Lb is an independently selected from a linking moiety comprising 0 to 10 main chain atoms, optionally substituted; each of Za and Zb is an independently selected complexing moiety comprising at least one nitrogen atom; either both or one of Za and/or Zb is coordinatively bonded to a respective transition metal complex Ma Va and Mb Vb through said nitrogen atom, wherein each of Ma and Mb is an independently selected transition metal, and each of Va and Vb is an independently selected valence group.

摘要翻译： 本发明涉及具有通式（1）的化合物：其中La和Lb各自独立地选自包含0至10个主链原子的连接部分，任选取代的; Za和Zb中的每一个是包含至少一个氮原子的独立选择的络合部分; Za和/或Zb中的任一个或Zb通过所述氮原子与相应的过渡金属络合物Ma Va和Mb Vb配位键合，其中Ma和Mb各自为独立选择的过渡金属，Va和Vb中的每一个为 独立选择的价电子基团。

公开(公告)号：US07807835B2

公开(公告)日：2010-10-05

申请号：US12413214

申请日：2009-03-27

申请人： Hong Xie ,  Zhiqiang Gao

发明人： Hong Xie ,  Zhiqiang Gao

IPC分类号： C07F15/00 ,  C12Q1/26

CPC分类号： C07F15/0026

摘要： Compositions and methods for electrochemical detection of an analyte comprising a transition metal compound wherein M is a metallic element that can form a coordinate bond to nitrogen; R and R′ are coordinated to M at their nitrogen atoms; L is a linking ligand; Z is chlorine or bromine; m can be from 1 to 6 and X is an anion, or combination of anions, that balances the charge m. Also provided are electrochemical tags and methods of detection.

摘要翻译： 用于电化学检测包含过渡金属化合物的分析物的组合物和方法，其中M是能够形成与氮的配位键的金属元素; R和R'在氮原子上与M配位; L是连接配体; Z是氯或溴; m可以是1至6，X是阴离子或阴离子的组合，其平衡电荷m。 还提供了电化学标签和检测方法。

公开(公告)号：US20100194409A1

公开(公告)日：2010-08-05

申请号：US12377404

申请日：2007-08-14

申请人： Zhiqiang Gao ,  Yi Fan ,  Xiantong Chen ,  Jinming Kong

发明人： Zhiqiang Gao ,  Yi Fan ,  Xiantong Chen ,  Jinming Kong

IPC分类号： G01R27/08 ,  C01B31/00

CPC分类号： G01N33/5438 ,  C12Q1/6825 ,  C12Q2565/607 ,  C12Q2563/155

摘要： The invention provides a method of electrically detecting a biological analyte molecule by means of a pair of electrodes. The electrodes are arranged at a distance from one another within a sensing zone. A capture molecule, which has an affinity to the analyte molecule and which is capable of forming a complex with the analyte molecule, is immobilised on an immobilisation unit. The immobilisation unit is contacted with a solution suspected to comprise the analyte molecule. The analyte molecule is allowed to form a complex with the capture molecule. The invention also provides a probe defined by a nanoparticulate tag that comprises or consists of electrically conducting matter that is capable of chemically interacting with the analyte molecule. In the method of the invention the electrically conducting nanoparticulate tag is added. Thereby the electrically conducting nanoparticulate tag is allowed to associate to the complex formed between the capture molecule and the analyte molecule. The presence of the analyte molecule is determined based on an electrical characteristic, influenced by the electrically conducting nanoparticulate tag, of a region in the sensing zone.

摘要翻译： 本发明提供一种通过一对电极电生物分析物分子的电学检测方法。 电极在感测区内相互间隔一定距离。 捕获分子与分析物分子具有亲和性，并且能够与分析物分子形成复合物，被固定在固定化单元上。 固定化单元与疑似包含分析物分子的溶液接触。 允许分析物分子与捕获分子形成复合物。 本发明还提供由纳米颗粒标签定义的探针，其包含能够与分析物分子进行化学相互作用的导电物质或由导电物质组成。 在本发明的方法中，添加导电纳米颗粒标签。 由此，导电纳米颗粒标签被允许与形成在捕获分子和分析物分子之间的复合物结合。 分析物分子的存在基于感测区域中的区域的导电纳米颗粒标签的电特性来确定。

公开(公告)号：US07655404B2

公开(公告)日：2010-02-02

申请号：US11568154

申请日：2004-04-29

申请人： Zhiqiang Gao ,  Hong Xie ,  Yuan Hong Yu

发明人： Zhiqiang Gao ,  Hong Xie ,  Yuan Hong Yu

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： B82Y15/00 ,  B82Y30/00 ,  C12Q1/001 ,  C12Q1/26 ,  C12Q1/6825 ,  G01N33/5438 ,  C12Q2565/519 ,  C12Q2563/113 ,  C12Q2523/115

摘要： A method for the detection and/or quantification of at least one target nucleic acid or target polypeptide in a sample of nucleic acids or polypeptides comprising the steps of: a) providing a sample comprising nucleic acids or polypeptides; b) labeling the nucleic acids or polypeptides with a ligand conjugate, the ligand conjugate comprising a first element binding to the nucleic acids or polypeptides and a second element which is a capture ligand; c) contacting the nucleic acid-ligand conjugates or polypeptide-ligand conjugates with at least one capture probe, the capture probe hybridizing with or binding to at least one target nucleic acid or target polypeptide; d) adding i) an oxidoreductase enzyme, wherein the oxidoreductase enzyme is recognized by the capture ligand, or ii) a complex comprising an oxidoreductase enzyme bound to a capture receptor, the capture receptor capable of binding to the capture ligand; e) adding a redox polymer, the redox polymer binding to the oxidoreductase enzyme, thereby resulting in the transfer of electrons from the enzyme via the redox polymer to an electrode surface; and f) detecting and/or quantifying the presence of the target nucleic acid(s) or target polypeptide(s).

摘要翻译： 一种用于检测和/或定量核酸或多肽样品中至少一种靶核酸或靶多肽的方法，包括以下步骤：a）提供包含核酸或多肽的样品; b）用配体缀合物标记核酸或多肽，所述配体缀合物包含与核酸或多肽结合的第一元件和作为捕获配体的第二元件; c）使所述核酸 - 配体缀合物或多肽 - 配体缀合物与至少一种捕获探针，所述捕获探针与至少一种靶核酸或靶多肽杂交或与至少一种靶核酸多肽结合; d）添加i）氧化还原酶，其中所述氧化还原酶被所述捕获配体识别，或ii）包含与捕获受体结合的氧化还原酶的复合物，所述捕获受体能够结合所述捕获配体; e）加入氧化还原聚合物，氧化还原聚合物与氧化还原酶结合，从而导致电子从酶经由氧化还原聚合物转移到电极表面; 和f）检测和/或定量靶核酸或靶多肽的存在。

公开(公告)号：US07563588B2

公开(公告)日：2009-07-21

申请号：US10577292

申请日：2004-10-25

申请人： Zhiqiang Gao ,  Guolin Xu ,  Yi-Ru Jackie Ying ,  Mohamed Shariff Mohamed Arshad ,  Fang Xie

发明人： Zhiqiang Gao ,  Guolin Xu ,  Yi-Ru Jackie Ying ,  Mohamed Shariff Mohamed Arshad ,  Fang Xie

IPC分类号： C12Q1/54

CPC分类号： B01D69/141 ,  C12N9/0006 ,  C12Q1/002 ,  C12Q1/003 ,  C12Q1/004 ,  C12Q1/005 ,  C12Q1/26 ,  C12Y101/03004 ,  G01N33/5438

摘要： An electrically non-conductive, nanoparticulate membrane comprising nanoparticles of at least one inorganic oxide of an element selected from Group IA, IIA, IIIA, IVA, IB, IIB, IIIB, IVAB, VB, VIB, VIIB or VIIIB of the Periodic Table, and wherein an oxidoreductase enzyme and a polymeric redox mediator capable of transferring electrons are diffusibly dispersed in said nanoparticulate membrane.

摘要翻译： 包含选自元素周期表的IA，IIA，IIIA，IVA，IB，IIB，IIIB，IVAB，VB，VIB，VIIB或VIIIB族元素的至少一种无机氧化物的纳米颗粒的非导电纳米颗粒膜， 并且其中能够转移电子的氧化还原酶和聚合物氧化还原介体可扩散地分散在所述纳米颗粒膜中。