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175 条记录 (耗时 0.034 秒)

    Structures for enhanced detection of fluorescence
    21.
    发明授权
    Structures for enhanced detection of fluorescence 有权
    用于增强荧光检测的结构

    公开(公告)号：US07988918B2

    公开(公告)日：2011-08-02

    申请号：US12261447

    申请日：2008-10-30

    申请人： Andres Fernandez

    发明人： Andres Fernandez

    IPC分类号： G01N21/64

    CPC分类号： G01N21/6428 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441

    摘要： Substrates are provided for use in the detection, identification and analysis of biologic or chemical samples that are labeled with a fluorescent label, in which the plane of maximum fluorescence is displaced from a reflective substrate surface so that the intensity maximum of the standing wave interference pattern of incident and reflected probe radiation is enhanced. The format of the substrates includes substantially planar surfaces as well as substrates with introduced variations to the substrate surface, e.g., depressions, wells, pedestals and the like, disposed in arrays or other similar structures such that one or more fluorophore-comprising objects can be attached thereto.

    摘要翻译： 提供了用于检测，识别和分析用荧光标记标记的生物或化学样品的底物，其中最大荧光平面从反射衬底表面移位，使得驻波干涉图的强度最大值 的事件和反射探针辐射得到增强。 衬底的形式包括基本平坦的表面以及具有引入到衬底表面的变化的衬底，例如设置在阵列或其它类似结构中的凹陷，阱，基座等，使得一个或多个含荧光体的物体可以是 附在其上。

    High throughput genome sequencing on DNA arrays
    24.
    发明申请
    High throughput genome sequencing on DNA arrays 审中-公开

    公开(公告)号：US20090118488A1

    公开(公告)日：2009-05-07

    申请号：US11981793

    申请日：2007-10-31

    申请人： Radoje T. Drmanac ,  Matthew Callow ,  Snezana Drmanac

    发明人： Radoje T. Drmanac ,  Matthew Callow ,  Snezana Drmanac

    IPC分类号： C07H21/04

    CPC分类号： C12Q1/6874 ,  C12N15/64 ,  C12N15/66 ,  Y10T436/143333 ,  C12Q2521/313 ,  C12Q2525/191 ,  C12Q2525/151 ,  C12Q2525/131 ,  C12Q2565/518 ,  C12Q2533/107 ,  C12Q2531/125

    摘要： The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new, e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both “upstream” and “downstream” of the adaptors, identification of entire target sequences may be accomplished.

    Nucleic acid analysis by random mixtures of non-overlapping fragments
    30.
    发明授权
    Nucleic acid analysis by random mixtures of non-overlapping fragments 有权

    公开(公告)号：US11414702B2

    公开(公告)日：2022-08-16

    申请号：US16730829

    申请日：2019-12-30

    申请人： Complete Genomics, Inc.

    发明人： Radoje Drmanac

    IPC分类号： C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/682 ,  C12Q1/6837 ,  C07H21/04 ,  C07K1/04 ,  G01N15/14

    摘要： The invention provides methods and kits for ordering sequence information derived from one or more target polynucleotides. In one aspect, one or more tiers or levels of fragmentation and aliquoting are generated, after which sequence information is obtained from fragments in a final level or tier. Each fragment in such final tier is from a particular aliquot, which, in turn, is from a particular aliquot of a prior tier, and so on. For every fragment of an aliquot in the final tier, the aliquots from which it was derived at every prior tier is known, or can be discerned. Thus, identical sequences from overlapping fragments from different aliquots can be distinguished and grouped as being derived from the same or different fragments from prior tiers. When the fragments in the final tier are sequenced, overlapping sequence regions of fragments in different aliquots are used to register the fragments so that non-overlapping regions are ordered. In one aspect, this process is carried out in a hierarchical fashion until the one or more target polynucleotides are characterized, e.g. by their nucleic acid sequences, or by an ordering of sequence segments, or by an ordering of single nucleotide polymorphisms (SNPs), or the like.