公开(公告)号：US4152208A

公开(公告)日：1979-05-01

申请号：US858129

申请日：1977-12-06

申请人： Hoda A. Guirgis

发明人： Hoda A. Guirgis

IPC分类号： A61K35/12 ,  A61K35/14 ,  A61K35/17 ,  C12K9/00

CPC分类号： A61K35/17 ,  A01N1/0226

摘要： The viability of leukocytes or specific fractions of leukocytes is maintained by drawing blood from a donor or patient into a mixture of an anticlotting agent and a basic salt solution (BSS) or a minimum essential medium (MEM) which solution or medium sustains the viability of the leukocytes in the blood, then layering the blood-MEM or BSS mixture on a Ficoll-Hypaque solution and centrifuging to separate the leukocytes. The resulting leukocytes are diluted to about 0.5.times.10.sup.6 viable cells per ml. in BSS or MEM containing antibiotics and fetal calf serum or serum substitutes equivalent thereto. The resulting suspension is transferred into sterile containers at room temperature so that each container has about 2-10.times.10.sup.6 cells. The containers are then packed into insulated containers and are ready for shipping.

摘要翻译： 通过将供体或患者的血液从抗渗剂和碱性盐溶液（BSS）或最低限度必需培养基（MEM）的混合物中维持白细胞或白细胞的特定部分的存活力，该溶液或培养基维持 血液中的白细胞，然后将血液MEM或BSS混合物分层在Ficoll-Hypaque溶液上并离心以分离白细胞。 将所得白细胞稀释至约0.5×10 6个存活细胞/ ml。 在含有抗生素和胎牛血清或血清替代物的BSS或MEM中。 将得到的悬浮液在室温下转移到无菌容器中，使得每个容器具有约2-10×10 6个细胞。 然后将容器包装在绝缘容器中并准备运输。

公开(公告)号：US4092425A

公开(公告)日：1978-05-30

申请号：US822742

申请日：1977-08-08

申请人： Dale A. Stringfellow

发明人： Dale A. Stringfellow

IPC分类号： A61K31/557 ,  A61K31/215 ,  A61K31/19 ,  C12K9/00

CPC分类号： A61K31/557 ,  Y10S435/811

摘要： The present invention provides a method for treatment of hyporeactivity to interferon induction in interferon producing animals or animal cells which comprises administering a hyporeactive interferon stimulating prostaglandin (HIS-PG) to an interferon producing animal or animal cells having a hyporeactive (decreased) interferon response. Hyporeactive interferon stimulating prostaglandins (HIS-PG) refers to those prostaglandin-type compounds which are useful in alleviating the decreased ability of interferon producing animals or animal cells to produce interferon following administration of repeated doses of an interferon inducer or as a consequence of viral infection or neoplasia.

摘要翻译： 本发明提供了一种用于治疗产生干扰素的动物或动物细胞中的干扰素诱导的低反应性的方法，其包括将具有低反应性（降低的）干扰素应答的产生干扰素的动物细胞刺激前列腺素（HIS-PG）的低反应性干扰素。 刺激前刺激素的前列腺素（HIS-PG）是指在施用重复剂量的干扰素诱导剂后或作为病毒感染的结果时，可用于减轻产生干扰素的动物或动物细胞产生干扰素的能力降低的前列腺素型化合物 或瘤形成。

公开(公告)号：US4085203A

公开(公告)日：1978-04-18

申请号：US670448

申请日：1976-03-25

申请人： Ronald Charles Telling ,  Roy John Passingham ,  Brian Lewis Kitchener ,  David George Hopkinson

发明人： Ronald Charles Telling ,  Roy John Passingham ,  Brian Lewis Kitchener ,  David George Hopkinson

IPC分类号： C12N5/00 ,  C12N7/00 ,  A61K39/12 ,  C12K9/00

CPC分类号： C12N7/00 ,  C12N5/0075 ,  C12N2531/00 ,  C12N2533/10 ,  C12N2533/50 ,  C12N2770/32151

摘要： A cell culture system which comprises living eucaryotic cells, such as those of human or animal origin, or mycophyta dispersed within a solid carrier body or bed, which carrier consists of porous or particulate material capable of retaining the cells while allowing liquid media to pass through or to have contact with the said cells, and processes for preparing and maintaining such culture systems, particularly for the purpose of virus propagation.

摘要翻译： 一种细胞培养系统，其包括诸如人或动物来源的真核细胞或分散在固体载体体或床内的真核细胞，该载体由能够保留细胞同时允许液体介质通过的多孔或颗粒材料组成 或与所述细胞接触，以及用于制备和维持这种培养系统的方法，特别是用于病毒繁殖的目的。

公开(公告)号：US4070243A

公开(公告)日：1978-01-24

申请号：US718807

申请日：1976-08-30

申请人： Marius C. Teodorescu ,  Eugene P. Mayer ,  Sheldon Dray

发明人： Marius C. Teodorescu ,  Eugene P. Mayer ,  Sheldon Dray

IPC分类号： G01N33/50 ,  C12K1/02 ,  C12K9/00

CPC分类号： G01N33/5094

摘要： A method for identification of cell subpopulations in a population of cells which are morphologically alike, based on the ability of selected strains of bacteria to bind to the cell surface when they are brought in close contact.Selected strains of naturally occurring or artificially obtained bacteria of different species or genuses, having an affinity to bind to different subpopulations of a cell mixture, are brought into contact by centrifugation or the like with a mixture of cells such as leukocytes, the mixture is resuspended and the cells having bacteria attached are identified under the microscope. The method is useful particularly for the identification of B cells, several subpopulations of T cells, and the differentiation among leukemic cell populations.

摘要翻译： 基于当所选菌株与细胞表面紧密接触时结合的能力，鉴定细胞群体中形态学相似的细胞亚群的方法。

公开(公告)号：US4013507A

公开(公告)日：1977-03-22

申请号：US547234

申请日：1975-02-05

申请人： Alan Rembaum

发明人： Alan Rembaum

IPC分类号： A61K31/785 ,  C08G73/02 ,  C12N5/00 ,  C12N5/06 ,  C12B1/00 ,  C12H1/04 ,  C12K9/00

CPC分类号： C12N5/0093 ,  A61K31/785 ,  C08G73/0226 ,  Y10S435/948

摘要： Ionene polymers of the structure ##STR1## WHERE X AND Y ARE INTEGERS FROM 3 TO 16, Z.sup.- is an anion such as a halogen and n is an integer from 50 to 150 are found to bind negatively charged mammalian cells such as malignant cells and can be utilized to selectively inhibit the growth of malignant cells in vitro.

摘要翻译： 结构图示的X射线衍生物，其中X和Y是3至16的整数，Z - 是阴离子，例如卤素，n是50至150的整数，可以结合带负电荷的哺乳动物细胞如恶性 细胞并可用于选择性地抑制体外恶性细胞的生长。

公开(公告)号：US4007087A

公开(公告)日：1977-02-08

申请号：US623543

申请日：1975-10-17

申请人： Ronald J. Ericsson

发明人： Ronald J. Ericsson

IPC分类号： A01N1/00 ,  A01N1/02 ,  A61K35/52 ,  C12K9/00

CPC分类号： A61K35/52

摘要： The percentage of the motile sperm of semen which survives frozen storage is increased by a process wherein the motile sperm is separated from the immotile sperm and the non-sperm components of the semen prior to storage. An insulated collection kit for collecting semen protects the semen from the adverse effects of handling and thermal shock.

摘要翻译： 在存储之前，通过一种方法将运动精子与精液与非精子组分分离，从而可以增加存活于冷冻储存中的精液活力精子的百分数。 用于收集精液的绝缘收集套件保护精液免受处理和热冲击的不利影响。

公开(公告)号：US4007086A

公开(公告)日：1977-02-08

申请号：US543508

申请日：1975-01-23

申请人： Ramon D. Hamilton

发明人： Ramon D. Hamilton

IPC分类号： C07K14/555 ,  C12K9/00 ,  A61K45/02 ,  B01J1/10

CPC分类号： C07K14/555 ,  Y10S435/811

摘要： An improved method for producing interferon which comprises irradiating interferon-producing human or animal cells in vitro with ultraviolet irradiation.

摘要翻译： 一种用于生产干扰素的改进方法，其包括用紫外线照射在体外辐射产生干扰素的人或动物细胞。

公开(公告)号：US3926723A

公开(公告)日：1975-12-16

申请号：US49560974

申请日：1974-08-08

申请人： MASSACHUSETTS INST TECHNOLOGY

发明人： GREEN HOWARD ,  RHEINWALD JAMES G

IPC分类号： C12N5/00 ,  C12K9/00 ,  C12B3/08

CPC分类号： C12N5/0018 ,  C12N2500/34

摘要： A cell culture medium is disclosed which contains secondary sources of glucose comprising glucose-containing oligosaccharides or polysaccharides and hydrolytic enzymes (glucosidases) to liberate free glucose. Starch, for example, can be added to a culture medium to provide the controlled release of glucose when a serum supplement such as fetal calf serum, which contains the hydrolytic enzymes amylase and maltase, is used. Amylase breaks the starch down into glucose and maltose, and maltase converts the remaining maltose to glucose. The rate of glucose release can be controlled by regulating the amount of glucosidase activity present in the medium.

公开(公告)号：US3871954A

公开(公告)日：1975-03-18

申请号：US43091874

申请日：1974-01-04

申请人： NAT RES DEV

发明人： ZUCKERMAN ARIE JEREMY

IPC分类号： C12N5/071 ,  C12N7/08 ,  C12K9/00

CPC分类号： C12N7/00 ,  C12N5/067 ,  C12N2770/32364

摘要： A method for producing a human embryo liver cell line which comprises disaggregating human embryo liver, suspending the cells in a growth medium, replacing this with fresh medium if it becomes acid, and allowing a confluent sheet of spindle-shaped cells of the cell line to form. The cells of the cell line, which are designated by A.T.C.C. No. CL99, may be sub-cultured and may be used as the basis of a cell-culture system for the culturing of mammalian cells in tissue culture. Viruses may be cultivated in a culture system based on cells of the cell line to provide antigenic material for the preparation of vaccines.

摘要翻译： 一种生产人胚胎肝细胞系的方法，其包括将人胚肝分解，将细胞悬浮于生长培养基中，如果其变为酸，则用新鲜培养基代替该胚细胞，并将细胞系的纺锤形细胞的汇合片 形成。 细胞系的细胞，由A.T.C.C. 编号CL99可以被亚培养，并且可以用作组织培养中哺乳动物细胞培养的细胞培养系统的基础。 可以在基于细胞系的细胞的培养系统中培养病毒，以提供用于疫苗制备的抗原材料。

公开(公告)号：US3698458A

公开(公告)日：1972-10-17

申请号：US3698458D

申请日：1971-02-11

申请人： US ARMY

发明人： HILL WAYNE E

IPC分类号： A47J43/14 ,  C12K7/00 ,  C12K9/00

CPC分类号： A47J43/14

摘要： An embryo stripper utilized in combination with a conventional egg breaker comprising a sloped tray mounted under the egg breaker to receive the embryo containing sacs, a trough having a slope traverse to the slope of the tray in juxtaposition to the tray in order to receive the embryo containing sacs, funnel means extending downward from one end of the trough to conduct the embryo containing sacs to a cutter assembly. The cutter assembly slashes the sacs to liberate the embryos. The embryos are retained on collecting means suitably designed to allow the sacs and allantoic fluids to drop into a waste chute below.

摘要翻译： 一种与常规蛋清器组合使用的胚胎剥离器，其包括安装在蛋破碎器下方的倾斜托盘以接收含胚囊的槽，该槽具有与托盘并列放置以便接收胚胎的坡度横穿到托盘的斜面 漏斗装置从槽的一端向下延伸以将含胚囊传导到切割器组件。 切割器组件切割囊以释放胚胎。 胚胎保留在适当设计成允许囊和尿囊液落入下面的废物斜槽的收集装置上。