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    METHODS FOR PRODUCTION OF BIOPRODUCTS
    22.
    发明公开
    METHODS FOR PRODUCTION OF BIOPRODUCTS 审中-公开

    公开(公告)号：US20240344089A1

    公开(公告)日：2024-10-17

    申请号：US18634947

    申请日：2024-04-14

    申请人： SUPERBREWED FOOD INC.

    发明人： ALAN KARPOL ,  AHARON M. EYAL ,  BRYAN P. TRACY

    IPC分类号： C12P1/04 ,  C12N1/20

    CPC分类号： C12P1/04 ,  C12N1/20

    摘要： Provided is a method for producing a bioproduct, comprising culturing at least one target microorganism in a target growth medium comprising cells of a strictly anaerobic bacterium, the cells comprising protein at a crude protein concentration of at least 60 wt % of a total dry weight of the cells, wherein at least 50% of a total number of the cells are dead cells.

    L-ARGININE-GLYCINE AMIDINOTRANSFERASE AND USE THEREOF IN THE PRODUCTION OF GUANIDINOACETIC ACID
    23.
    发明公开
    L-ARGININE-GLYCINE AMIDINOTRANSFERASE AND USE THEREOF IN THE PRODUCTION OF GUANIDINOACETIC ACID 审中-公开

    公开(公告)号：US20240344038A1

    公开(公告)日：2024-10-17

    申请号：US18415827

    申请日：2024-01-18

    申请人： JIANGNAN UNIVERSITY

    发明人： Meijuan XU ,  Zhiming RAO ,  Yaxin LIAO ,  Haofei XU ,  Xian ZHANG ,  Taowei YANG

    IPC分类号： C12N9/10 ,  C12N15/70 ,  C12P13/04

    CPC分类号： C12N9/1003 ,  C12N15/70 ,  C12P13/04 ,  C12Y201/04001

    摘要： The present invention discloses an L-arginine-glycine amidinotransferase and use thereof in the production of guanidinoacetic acid. In the present invention, through combined multi-site amino acid mutation, a technical effect of significantly improved enzyme activity of the mutant AkAGATT225Q/A258P/L278K than that of the wild-type strain is achieved, providing an application value for large-scale production of guanidinoacetic acid in industry. When the L-arginine-glycine amidinotransferase mutant constructed in the present invention is used in the production of guanidinoacetic acid, by optimizing the conversion conditions, the yield of guanidinoacetic acid is up to 21.4 g/L and the conversion rate is 90.4%, after 24 hrs of reaction in a 1 L reaction system. Compared with the production of guanidinoacetic acid with the raw enzyme, the yield is increased by 49.6%.

    METHOD TO PRODUCE COLLAGEN AS THERAPEUTICS AND BIOMATERIALS
    26.
    发明公开
    METHOD TO PRODUCE COLLAGEN AS THERAPEUTICS AND BIOMATERIALS 审中-公开

    公开(公告)号：US20240336947A1

    公开(公告)日：2024-10-10

    申请号：US18626636

    申请日：2024-04-04

    申请人： University of Kentucky Research Foundation

    发明人： Houfu Guo

    IPC分类号： C12P21/02 ,  C12N9/10

    CPC分类号： C12P21/02 ,  C12N9/1051 ,  C12Y204/01066

    摘要： Humans and Acanthamoeba Polyphaga Mimivirus share numerous homologous genes, including collagens and collagen-modifying enzymes. To explore the homology, a genome-wide comparison was performed between human and mimivirus using DELTA-BLAST (Domain Enhanced Lookup Time Accelerated BLAST) and identified 190 new mimiviral proteins that share homology with 1236 human proteins. To gain functional insights into mimiviral proteins, the human protein homologs were organized into Gene Ontology (GO) and REACTOME pathways to build a functional network. Collagen and collagen-modifying enzymes form the largest subnetwork with most nodes. Further analysis of this subnetwork identified a putative collagen glycosyltransferase R699. Protein expression test suggested that R699 is highly expressed in E coli, unlike the human collagen-modifying enzymes. Enzymatic activity assays showed that R699 catalyzes the conversion of galactosyl-hydroxylysine to glucosyl-galactosyl-hydroxylysine on collagen using UDP-glucose as a sugar donor, suggesting R699 is a mimiviral collagen galactosylhydroxylysyl glucosyltransferase (GGT). Structural study of R699 produced the first crystal structure of a collagen GGT with uridine diphosphate glucose (UDP-Glc). Sugar moiety of the UDP-Glc resides in a previously unrecognized pocket. Mn2+ coordination and nucleoside-diphosphate binding site are conserved among GGT family members and critical for R699's collagen GGT activity. To facilitate further analysis of human and mimiviral homologous proteins, we presented an interactive and searchable genome-wide comparison app for quickly browsing of human and Acanthamoeba Polyphaga Mimivirus homologs, which is available at RRID Resource ID: SCR_022140 or guolab.shinyapps.io/app-mimivirus-publication/.

    In vitro glycosylation of proteins and enzymes
    28.
    发明授权
    In vitro glycosylation of proteins and enzymes 有权

    公开(公告)号：US12110532B2

    公开(公告)日：2024-10-08

    申请号：US18170045

    申请日：2023-02-16

    申请人： Design-Zyme LLC

    发明人： Peter Albert Petillo ,  Dwight O'Dell Deay, III

    IPC分类号： C12Q1/00 ,  C07K1/00 ,  C07K1/107 ,  C12N9/02 ,  C12N9/04 ,  C12N9/06 ,  C12P21/00

    CPC分类号： C12Q1/005 ,  C07K1/006 ,  C07K1/1077 ,  C12N9/0004 ,  C12N9/0006 ,  C12P21/005

    摘要： The present invention is broadly concerned with new in vitro glycosylation methods that provide rational approaches for producing glycosylated proteins, and the use of glycosylated proteins. In more detail, the present invention comprises methods of glycosylating a starting protein having an amino sidechain with a nucleophilic moiety, comprising the step of reacting the protein with a carbohydrate having an oxazoline moiety on the reducing end thereof, to covalently bond the amino sidechain of the starting protein with the oxazoline moiety, wherein the glycosylated protein substantially retains the structure and function of the starting protein. Target proteins include oxidase, oxidoreductase and dehydrogenase enzymes. The glycosylated proteins advantageously have molecular weights of at least about 7500 Daltons. In a further embodiment, the present invention concerns the use of glycosylated proteins, fabricated by the methods disclosed herein, in the assembly of amperometric biosensors.

    Methods and systems using ionophores to control contamination in fermentation of gaseous substrates
    29.
    发明授权
    Methods and systems using ionophores to control contamination in fermentation of gaseous substrates 有权

    公开(公告)号：US12110529B2

    公开(公告)日：2024-10-08

    申请号：US16471453

    申请日：2017-12-14

    申请人： Synata Bio, Inc.

    发明人： Jennine B. Terrill

    IPC分类号： C12P7/06 ,  C12M1/00 ,  C12P7/40

    CPC分类号： C12P7/065 ,  C12M21/12 ,  C12M43/02

    摘要： Provided is a method of anaerobically fermenting a gaseous substrate to form a liquid product, the method comprising: (a) introducing the gaseous substrate into a bio-reactor, the gaseous substrate comprising at least one of the following constituents: carbon monoxide, carbon dioxide, and hydrogen, (b) the bio-reactor comprising a fermentation broth therein, the fermentation broth containing at least two types of microorganisms, one type comprising at least one fermenting species, and the other type comprising at least one competing species; (c) introducing at least one type of ionophore into the reactor, the ionophore having selectivity for preferentially inhibiting the at least one competing species from growing and/or producing an undesired product; and (d) allowing the gaseous substrate to ferment by exposure to the at least one fermenting species, to produce the liquid product and a system for doing the same.