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1092 条记录 (耗时 0.026 秒)

    RAPID MILK SAMPLE PREPARATION METHOD COMPATIBLE WITH MOLECULAR TESTS
    23.
    发明公开
    RAPID MILK SAMPLE PREPARATION METHOD COMPATIBLE WITH MOLECULAR TESTS 审中-公开

    公开(公告)号：US20240240264A1

    公开(公告)日：2024-07-18

    申请号：US18557791

    申请日：2022-04-28

    申请人： Zoetis Services LLC

    发明人： Sridhar Velineni

    IPC分类号： C12Q1/689 ,  C12Q1/6806 ,  C12Q1/6851

    CPC分类号： C12Q1/689 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q2600/16

    摘要： The present invention provides a method of preparing a milk sample for a downstream nucleic acid amplification process. This method includes: subjecting a milk sample comprising at least one suspected bacterial pathogen implicated in mastitis to contact with uncoated magnetic beads; incubating the milk sample in the presence of the uncoated magnetic beads to allow bacterial cells in the milk sample to bind to the beads, thereby forming a bead-bacterium complex; subjecting the bead-bacterium complex to a magnet such that the bead-bacterium complex is separated from a milk sample supernatant; removing the milk sample supernatant; directly resuspending the separated bead-bacterium complex in an aqueous solution to release the bead-bound bacteria into the solution; and employing an aliquot of the bead resuspension or bead-free supernatant following separation of the beads as a template for primer-specific downstream nucleic acid amplification.

    MICRO-RNA DETECTION METHOD AND KIT
    24.
    发明公开
    MICRO-RNA DETECTION METHOD AND KIT 审中-公开

    公开(公告)号：US20240229121A1

    公开(公告)日：2024-07-11

    申请号：US18561284

    申请日：2023-03-21

    申请人： ZHEJIANG LAB

    发明人： PEIXIANG MA ,  MINGTIAN ZHONG ,  LU DANG ,  BAICHENG HUANG

    IPC分类号： C12Q1/6851 ,  C12Q1/6823

    CPC分类号： C12Q1/6851 ,  C12Q1/6823 ,  C12Q2600/178

    摘要： The present invention discloses a microRNA detection method and kit for rapid detection of microRNA nucleic acids in biological and environmental samples, including microRNA tailing and a Cpf1 detection system suitable for rapid detection. The present invention is the first to use a combination of microRNA tailing and Cpf1 detection to detect microRNAs, with the advantages of high sensitivity, strong specificity, short time consumption, high throughput, direct interpretation by the naked eye, no dependence on large-scale experimental equipment and the like. These advantages make the detection method developed by the present invention convenient for rapid detection, and identification and diagnosis of microRNAs in biological and environmental samples at a clinical front line.

    METHODS AND COMPOSITIONS FOR TREATING CELL SENESCENCE ACCUMULATION RELATED DISEASE
    29.
    发明公开
    METHODS AND COMPOSITIONS FOR TREATING CELL SENESCENCE ACCUMULATION RELATED DISEASE 审中-公开

    公开(公告)号：US20240158861A1

    公开(公告)日：2024-05-16

    申请号：US18556472

    申请日：2022-04-22

    申请人： INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE) ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) ,  UNIVERSITE COTE D'AZUR

    发明人： Julien CHERFILS ,  Eric GILSON ,  Charlene ILTIS

    IPC分类号： C12Q1/6883 ,  C12Q1/6851 ,  G01N33/68

    CPC分类号： C12Q1/6883 ,  C12Q1/6851 ,  G01N33/6893 ,  C12Q2600/158

    摘要： The inventors have surprisingly demonstrated that GD3 positive senescent cells inhibit NK cell in vitro and in vivo while GD3 negative senescent cells is associated with NK cell functionality, both with human or murine cells. The inventors' results bring the proof of concept that GD3 expression may represent a Senescence-associated Immune Checkpoint (SIC) that determines senescent cell immunogenicity and identify GD3 and more generally SIC as a multi-hit target for age-associated diseases. Thus, GD3 may be a major step forward in the development of efficient anti-senescence immunotherapies. In particular, the present invention relates to a method for identifying whether a cell is in senescence process comprising the steps of: i) measuring the co-expression level of ST8Sia1 (GD3) with a senescence marker in a biological sample; ii) comparing the co-expression level measured at step i) with its predetermined reference value, and iii) concluding that the cell is in senescence process when the co-expression level of GD3 with a senescence marker is higher than its predetermined reference value or concluding that cell is not in senescence process when the co-expression level of GD3 with a senescence marker is lower or similar than its predetermined reference value. The present invention also relates a method for treating senescent cells accumulation related disease in a subject in need thereof, comprising administering to said subject a therapeutically effective amount of a GD3 inhibitor.