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    Polymerase compositions, methods of making and using same
    312.
    发明授权
    Polymerase compositions, methods of making and using same 有权

    公开(公告)号：US09976128B2

    公开(公告)日：2018-05-22

    申请号：US14754203

    申请日：2015-06-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter Vander Horn ,  Theo Nikiforov ,  Guobin Luo ,  Mindy Landes ,  Daniel Mazur ,  Eileen Tozer ,  Tommie Lincecum

    IPC: C12N9/12 ,  C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/686 ,  C12Y207/07007

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

    MODIFIED NUCLEOTIDES AND USES THEREOF
    315.
    发明申请
    MODIFIED NUCLEOTIDES AND USES THEREOF 审中-公开

    公开(公告)号：US20180119217A1

    公开(公告)日：2018-05-03

    申请号：US15561445

    申请日：2016-03-18

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES GMBH

    Inventor： Wolfgang HINZ ,  Steven MENCHEN ,  Ronald GRAHAM ,  Peter VANDER HORN ,  Earl HUBBELL ,  Christian WOEHLER ,  Roman ROZHKOV ,  Barnett ROSENBLUM

    IPC: C12Q1/6874

    CPC classification number: C12Q1/6874 ,  C12Q2565/607

    Abstract: In some embodiments, the disclosure relates generally to methods, as well as related, systems, compositions, kits and apparatuses, for nucleic acid analysis that involve the use of modified nucleotides, including terminator nucleotides and/or tagged nucleotides, in a template-dependent nucleotide incorporation reaction. In some embodiments, the nucleic acid analysis can be conducted at a single reaction site, or at a plurality of reaction sites in an array of reaction sites. Optionally, the array contains a plurality of reaction sites having about 1-100 million, or about 100-250 million, or about 200-500 million, or about 500-900 million, or more reaction sites. Optionally, each reaction site is in contact with, operatively coupled, or capacitively coupled to one or more sensors that are ion-sensitive FETs (isFETs) or chemically-sensitive FETs (chemFETs) sensors. Optionally, the reaction sites are in fluid communication with each other.

    LINKING METHODS, COMPOSITIONS, SYSTEMS, KITS AND APPARATUSES
    316.
    发明申请
    LINKING METHODS, COMPOSITIONS, SYSTEMS, KITS AND APPARATUSES 审中-公开

    公开(公告)号：US20180105805A1

    公开(公告)日：2018-04-19

    申请号：US15786223

    申请日：2017-10-17

    Applicant: Life Technologies Corporation

    Inventor： John DAVIDSON ,  Theo Nikiforov ,  Guobin Luo

    IPC: C12N11/10 ,  C12N9/12

    CPC classification number: C12N11/10 ,  C12N9/1241 ,  C12Q1/68 ,  G01N2333/9126

    Abstract: In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can be used to link the protein to a target compound, for example, to link an enzyme to a substrate. Similarly, the tether can be used to link the protein at or near a desired location on a surface. In one group of embodiments, the tether includes a polynucleotide and the target compound or location on the surface includes another polynucleotide that is capable of hybridizing to the tether. In such embodiments, the tether can be used to link the protein to the target compound or location using nucleic acid hybridization.

    Systems and methods for assessing images
    320.
    发明授权
    Systems and methods for assessing images 有权

    公开(公告)号：US09940707B2

    公开(公告)日：2018-04-10

    申请号：US14484080

    申请日：2014-09-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Haoning Fu

    IPC: G06K9/00 ,  G06T7/00 ,  G01N21/64

    CPC classification number: G06T7/0012 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2021/6441 ,  G06K9/00 ,  G06T2207/30072 ,  G06T2207/30168

    Abstract: Disclosed are systems and methods for assessing images in applications such as microscopic scanning of a slide having light emitting objects. In certain embodiments, such scanning can involve objects such as sequencing beads disposed on the slide to facilitate biological analysis such as nucleic acid sequencing. Also disclosed are certain embodiments where images of light emitting objects are assessed for image quality so as to facilitate a feedback response such as a corrective action. In certain embodiments, such assessment and correction can be performed in real-time during the scanning process, and can include re-acquisition of the assessed image. Also disclosed are certain embodiments where such assessment and correction can be triggered dynamically during the scan, or before start of the scan, so as to enhance the scanning performance, including scanning time and throughput.

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