公开(公告)号：US20130236913A1

公开(公告)日：2013-09-12

申请号：US13892011

申请日：2013-05-10

申请人： CHANG GUNG UNIVERSITY

发明人： Err-Cheng CHAN ,  Kuei-Tien CHEN ,  Jau-Song YU ,  Yu-Sun CHANG ,  Jinn-Shiun CHEN

IPC分类号： G01N33/92

CPC分类号： G01N33/92 ,  C07K14/47 ,  C12N9/14 ,  G01N33/57419

摘要： Embodiments relate to serological markers for detecting the colorectal cancer and applications of the serological markers. A phospholipid scramblase (PLSCR1), a stomatin-like protein 2 (STOML2) or a transport protein Sec61β (SEC61β) increases expression in the blood at the earlier stage of the colorectal cancer. Detecting the expression of the PLSCR1, STOML2 or SEC61β protein or an induced autoantibody of each protein in a blood sample is used to diagnose the colorectal cancer. Moreover, the serological marker improves the detection efficiency and the sensitivity in detecting the colorectal cancer and is used to predict the prognosis. The serological markers are applied in preparing a detection device or inhibiting the growth of the colorectal cancer cells.

摘要翻译： 实施方案涉及用于检测结肠直肠癌的血清学标志物和血清学标记物的应用。 卵磷脂酶（PLSCR1），类胡萝卜素蛋白2（STOML2）或运输蛋白Sec61beta（SEC61beta）增加了结肠直肠癌早期血液中的表达。 检测血液样品中PLSCR1，STOML2或SEC61beta蛋白的表达或每种蛋白质的诱导自身抗体用于诊断结肠直肠癌。 此外，血清学标记提高了检测结直肠癌的检测效率和灵敏度，并用于预测预后。 血清学标记物用于制备检测装置或抑制结肠直肠癌细胞的生长。

公开(公告)号：US20130225792A1

公开(公告)日：2013-08-29

申请号：US13600963

申请日：2012-08-31

申请人： HSIU-CHUAN YEN ,  SHIH-WEI CHEN

发明人： HSIU-CHUAN YEN ,  SHIH-WEI CHEN

IPC分类号： C07K14/00 ,  C12P21/02

CPC分类号： C12N9/1007 ,  C12Y201/01201

摘要： The present invention relates to a producing and purifying method of soluble recombinant COQ5 protein, which is expressed in soluble form by E. coli, under native conditions. The method is characterized by pre-treating bacterial lysate with low concentration of ionic detergent, such as SDS, before purification; and the purifying method is performed under native condition without using urea to avoid the problems of requiring lengthy processes to remove urea in purified protein solution or re-aggregation and precipitation of protein after removal of urea.

公开(公告)号：US20120231976A1

公开(公告)日：2012-09-13

申请号：US13241123

申请日：2011-09-22

申请人： Min-Hsien Wu ,  Shih-Siou Wang ,  Wan-Chen Tsai ,  Yu-Han Chang ,  Chia-Hsun Hsieh ,  Yen-Ting Liu ,  Zhanfeng Cui

发明人： Min-Hsien Wu ,  Shih-Siou Wang ,  Wan-Chen Tsai ,  Yu-Han Chang ,  Chia-Hsun Hsieh ,  Yen-Ting Liu ,  Zhanfeng Cui

IPC分类号： C40B60/14

CPC分类号： C40B60/12 ,  C12M23/16 ,  C12M41/00 ,  C12M41/12 ,  C12M41/40

摘要： A microfluidic chip for three-dimensional cell culture with high-throughput perfusion includes an array of cell culture units, each unit including a cell culture medium inlet hole connecting to one cell culture medium tank, at least one micro-bioreactor, at least one microchannel and at least one medium collection and analysis tank. Each medium collection and analysis tank is connected to an air chamber with an air channel and the air chamber has negative pressure source holes to generate negative pressure to drive the culture medium. The microfluidic chip also includes an intermediate plate connected to the bottom surface of the roof, and two bottom plates detachably assembled at the bottom of the intermediate plate. The first and second bottom surfaces have micro-bioreactors and cylindrical recessed slots and the intermediate plate has corresponding holes to achieve the goal of three-dimensional cell culture using minimum experimental resources with high-throughput perfusion.

摘要翻译： 用于具有高通量灌注的三维细胞培养的微流体芯片包括一系列细胞培养单元，每个单元包括连接到一个细胞培养基罐的细胞培养基入口孔，至少一个微生物反应器，至少一个微通道 和至少一个介质收集和分析罐。 每个介质收集和分析罐连接到具有空气通道的空气室，并且空气室具有负压源孔以产生负压以驱动培养基。 微流体芯片还包括连接到屋顶底面的中间板，以及可拆卸地组装在中间板的底部的两个底板。 第一和第二底面具有微生物反应器和圆柱形凹槽，并且中间板具有相应的孔，以使用具有高通量灌注的最小实验资源实现三维细胞培养的目标。

公开(公告)号：US20120195821A1

公开(公告)日：2012-08-02

申请号：US13356925

申请日：2012-01-24

申请人： Chia-Liang SUN ,  Ching-Tang CHANG

发明人： Chia-Liang SUN ,  Ching-Tang CHANG

IPC分类号： C01B31/02 ,  B82Y40/00

CPC分类号： C01B31/0273 ,  B82Y30/00 ,  B82Y40/00 ,  C01B32/174 ,  C01B2204/06 ,  Y10S977/842

摘要： A method for forming graphene nanoribbons includes: (a) dispersing carbon nanotubes in a solvent to obtain a nanotube-dispersing solution; (b) adding an oxidant into the nanotube-dispersing solution to obtain a reaction solution; and (c) microwave heating the reaction solution and longitudinally unzipping the carbon nanotubes to form graphene nanoribbons.

摘要翻译： 形成石墨烯纳米带的方法包括：（a）将碳纳米管分散在溶剂中以获得纳米管分散溶液; （b）在纳米管分散溶液中加入氧化剂，得到反应溶液; 和（c）微波加热反应溶液并纵向解压缩碳纳米管以形成石墨烯纳米带。

公开(公告)号：US08148059B2

公开(公告)日：2012-04-03

申请号：US12610008

申请日：2009-10-30

申请人： Jau-Song Yu ,  Kai-Ping Chang

发明人： Jau-Song Yu ,  Kai-Ping Chang

IPC分类号： C12Q1/70

CPC分类号： G01N33/57407 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q2600/178

摘要： A method of detecting malignancy of nasopharyngeal carcinoma and a nasopharyngeal carcinoma malignancy biomarker are disclosed. Firstly, a specimen is obtained from a testee. Next, the specimen is tested for its MIP-3α expression level. Then, the MIP-3α expression level of the specimen is compared with that of a control. Finally, the malignancy of nasopharyngeal carcinoma is determined according to a relative MIP-3α expression level between the specimen and the control.

摘要翻译： 公开了一种检测鼻咽癌恶性肿瘤和鼻咽癌恶性肿瘤生物标志物的方法。 首先，从被测者获得标本。 接下来，测试样品的MIP-3α表达水平。 然后，将样品的MIP-3α表达水平与对照的MIP-3α表达水平进行比较。 最后，根据样本与对照组之间的相对MIP-3α表达水平确定鼻咽癌恶性肿瘤。

公开(公告)号：US20110306881A1

公开(公告)日：2011-12-15

申请号：US12926326

申请日：2010-11-10

申请人： Hao-Li Liu ,  Ming-Shi Lin

发明人： Hao-Li Liu ,  Ming-Shi Lin

IPC分类号： A61N7/00 ,  A61B8/00

CPC分类号： A61N7/02 ,  A61B8/485 ,  A61B8/5223 ,  A61B8/54 ,  A61B8/587 ,  A61B2017/00084 ,  A61B2090/378

摘要： The invention disclosed a high-intensity focused ultrasound thermal ablation apparatus having integrated temperature estimation and elastography for thermal lesion determination and the method thereof, using the different power to burn by the same focused ultrasound transducer, and then using the apparatus to measure the temperature and elasticity estimating by the relevant analysis method.

摘要翻译： 本发明公开了一种具有用于热病变确定的综合温度估计和弹性成像的高强度聚焦超声热消融装置及其方法，使用不同功率通过相同的聚焦超声换能器燃烧，然后使用该装置测量温度和 通过相关分析方法估计弹性。

公开(公告)号：US20110255604A1

公开(公告)日：2011-10-20

申请号：US13170736

申请日：2011-06-28

申请人： Jiann-Der Lee ,  Hao-Hang Hsu

发明人： Jiann-Der Lee ,  Hao-Hang Hsu

IPC分类号： H04N7/26

CPC分类号： H04N19/533

摘要： Motion estimation is efficient to reduce redundant information among successive frames in video compression applications. The blocks in the current frame can be replaced with the neighboring blocks in the spatial directions in the previous frame with small errors. Many types of motion estimation methods such as Block Matching Algorithm are widely used to take a balance between a good image quality and the computation complexity. A Block Matching Algorithm named as New Cellular Search (NCS) Algorithm utilizes two particular search patterns: HCSP and VCSP, in the horizontal and vertical directions to search the best motion vector. Three performance measurements including peak signal to noise ration (PSNR), Average Search Point (ASP), and Mean Square Error (MSE) are used to compare this new search algorithm with some major motion estimations like FS, TSS, CS, and NCDS. The NCS is very efficient in computation reduction while keeping the almost same picture quality.

摘要翻译： 运动估计在视频压缩应用中在连续帧之间减少冗余信息是有效的。 当前帧中的块可以用前一帧的空间方向上的相邻块替换为小错误。 许多类型的运动估计方法如块匹配算法被广泛用于在良好的图像质量和计算复杂度之间取得平衡。 名为新蜂窝搜索（NCS）算法的块匹配算法利用两种特定的搜索模式：HCSP和VCSP，在水平和垂直方向上搜索最佳运动矢量。 使用三个性能测量，包括峰值信噪比（PSNR），平均搜索点（ASP）和均方误差（MSE），以将这种新的搜索算法与诸如FS，TSS，CS和NCDS之类的一些主要运动估计进行比较。 NCS在减少计算中非常有效，同时保持几乎相同的图像质量。

公开(公告)号：US20110180778A1

公开(公告)日：2011-07-28

申请号：US12694600

申请日：2010-01-27

申请人： Ray-Ming Lin ,  Jhong-Hao Jiang ,  Bor-Ren Fang

发明人： Ray-Ming Lin ,  Jhong-Hao Jiang ,  Bor-Ren Fang

IPC分类号： H01L33/00

CPC分类号： H01L33/04 ,  H01L33/32

摘要： The present invention relates to a GaN series light-emitting diode structure, which includes a substrate; at least one GaN series layer formed over the substrate; subsequently an interface blocking structure composed of an n-type GaN series superlattice structure and a GaN series light-emitting layer, and a GaN series light-emitting layer are formed over the GaN series layer; and a p-type GaN series layer formed over the GaN series light-emitting layer. In the present invention, the radiative recombination efficiency is improved by introducing an interface blocking structure before the light-emitting layer under the epitaxial conditions of low temperature and pure nitrogen atmosphere.

摘要翻译： 本发明涉及一种GaN系发光二极管结构，其包括：基板; 在衬底上形成至少一个GaN系列层; 随后在GaN系列层上形成由n型GaN系超晶格结构和GaN系列发光层构成的界面阻挡结构和GaN系列发光层; 以及形成在GaN系发光层上的p型GaN系列层。 在本发明中，通过在低温纯氮气氛的外延条件下在发光层之前引入界面阻挡结构来提高辐射复合效率。

公开(公告)号：US07894888B2

公开(公告)日：2011-02-22

申请号：US12236558

申请日：2008-09-24

申请人： Hsiao-Lung Chan ,  Pei-Kuang Chao ,  Jia-Chen Jheng

发明人： Hsiao-Lung Chan ,  Pei-Kuang Chao ,  Jia-Chen Jheng

IPC分类号： A61B5/04

CPC分类号： A61B5/0404 ,  A61B5/0006 ,  A61B5/04325 ,  A61B5/681 ,  A61B2560/0468 ,  A61B2560/0475 ,  G06F19/00

摘要： A wristwatch worn by a user for measuring a three-lead ECG includes three electrodes placed separately on the front, either side, and back or strap thereof. The wristwatch further includes an electrode panel having the electrode on the front or either side of the watch, sensing elements, pressure, infrared or impedance detectors, and circuits. The electrode panel is capable of sensing the contact or press of fingers to trigger the ECG measuring. While the electrode in the back-side of the watch contacts the hand wearing the watch, the electrode and electrode panel on the front or either side of the watch are pressed by fingers from the other hand, and the electrode in the strap contacts the abdomen or left leg simultaneously. Thus, a three-lead ECG can be measured. ECG data can be transmitted to a personal or hospital computer by wireless networks or flash memory.

摘要翻译： 由用户测量三导联ECG的手表包括三个电极，分别放置在前面，其侧面和背面或背带上。 手表还包括电极板，其电极位于手表的前侧或两侧，感测元件，压力，红外线或阻抗检测器以及电路。 电极面板能够感测触摸或手指按压以触发ECG测量。 当手表背面的电极与手表接触时，手表的前面或两侧的电极和电极面板被另一只手按压，带子中的电极接触到腹部 或左腿同时。 因此，可以测量三导联ECG。 ECG数据可以通过无线网络或闪存传输到个人或医院的计算机。

公开(公告)号：US07803543B2

公开(公告)日：2010-09-28

申请号：US11624796

申请日：2007-01-19

申请人： Chiuan-Chian Chiou ,  Ji-Dung Luo

发明人： Chiuan-Chian Chiou ,  Ji-Dung Luo

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  C12Q1/6858 ,  C12Q2531/113 ,  C12Q2525/107 ,  C12Q2525/186 ,  C12Q2563/107 ,  C12Q2549/126 ,  C12Q2535/131

摘要： Disclosed herein is a method for determining whether a target polynucleotide sequence contained in a nucleic acid sample has nucleotide variation(s) in a selected region thereof, the steps of which involve the use of a pair of primers that allows the formation of a PCR product having a sequence covering that of the selected region of the target polynucleotide sequence via a PCR process, and a peptide nucleic acid (PNA) that acts as a PCR clamp as well as a sensor probe. Also disclosed herein is a kit for use in determining the presence of nucleotide variation(s) in the target polynucleotide sequence, which includes the pair of primers and the PNA.

摘要翻译： 本文公开了一种用于确定核酸样品中包含的靶多核苷酸序列是否在其选定区域具有核苷酸变异的方法，其步骤涉及使用允许形成PCR产物的一对引物 具有通过PCR过程覆盖靶多核苷酸序列的选定区域的序列，以及充当PCR夹的肽核酸（PNA）以及传感器探针。 本文还公开了用于确定靶多核苷酸序列中核苷酸变异存在的试剂盒，其包括一对引物和PNA。