公开(公告)号：US07226758B1

公开(公告)日：2007-06-05

申请号：US08182183

申请日：1992-09-17

Applicant: Leu-Fen H. Lin ,  Franklin D. Collins ,  Daniel H. Doherty ,  Jack Lile ,  Susan Bektesh

Inventor： Leu-Fen H. Lin ,  Franklin D. Collins ,  Daniel H. Doherty ,  Jack Lile ,  Susan Bektesh

IPC: C12N15/18 ,  C12N15/85 ,  C12N15/63 ,  C12N15/70

CPC classification number: A61K9/0085 ,  A61K38/00 ,  C07K14/475 ,  Y10S530/809 ,  Y10S530/839

Abstract: A novel neurotrophic factor referred to as glial cell line-derived neurotrophic factor (GDNF) has been identified and isolated from serum free growth conditioned medium of B49 glioblastoma cells. Rat and human genes encoding GDNF have been cloned and sequenced. A gene encoding GDNF has been subcloned into a vector, and the vector has been used to transform a host cell in order to produce biologically active GDNF in a recombinant DNA process.

Abstract translation: 已经鉴定了一种称为神经胶质细胞系衍生的神经营养因子（GDNF）的新型神经营养因子，并从B49胶质母细胞瘤细胞的无血清生长条件培养基中分离。 编码GDNF的大鼠和人类基因已被克隆并测序。 编码GDNF的基因已经被亚克隆到载体中，并且该载体已被用于转化宿主细胞，以便在重组DNA过程中产生生物活性的GDNF。

公开(公告)号：US06753165B1

公开(公告)日：2004-06-22

申请号：US09889273

申请日：2001-09-06

Applicant: George N. Cox ,  Daniel H. Doherty ,  Mary S. Rosendahl

Inventor： George N. Cox ,  Daniel H. Doherty ,  Mary S. Rosendahl

IPC: C07K1107

CPC classification number: C12P21/02 ,  A61K47/60 ,  C07K1/1133 ,  C07K14/505 ,  C07K14/56 ,  C07K14/61 ,  C07K14/72

Abstract: The present invention relates to novel methods of making soluble proteins having free cysteines in which a host cell is exposed to a cysteine blocking agent. The soluble proteins produced by the methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form pegylated proteins.

Abstract translation: 本发明涉及制备具有游离半胱氨酸的可溶性蛋白质的新方法，其中宿主细胞暴露于半胱氨酸阻断剂。 然后可以对通过该方法产生的可溶性蛋白质进行修饰以增加它们的有效性。 这样的修饰包括连接PEG部分以形成聚乙二醇化的蛋白质。

公开(公告)号：US06221376B1

公开(公告)日：2001-04-24

申请号：US08935268

申请日：1997-09-22

Applicant: Leu-Fen H. Lin ,  Franklin D. Collins ,  Daniel H. Doherty ,  Jack Lile ,  Susan Bektesh

Inventor： Leu-Fen H. Lin ,  Franklin D. Collins ,  Daniel H. Doherty ,  Jack Lile ,  Susan Bektesh

IPC: A61F200

CPC classification number: A61K9/0085 ,  A61K38/00 ,  C07K14/475 ,  Y10S530/809 ,  Y10S530/839

Abstract: A novel neurotrophic factor referred to as glial cell line-derived neurotrophic factor (GDNF) has been identified. Genes encoding GDNF have been cloned and sequenced. Methods and devices are disclosed for the delivery of GDNF using either an implantable cell which expresses human GDNF or a biocompatible semipermeable membrane containing genetically engineered cells which express and secrete the neurotrophic factor.

Abstract translation: 已经鉴定了称为神经胶质细胞系衍生的神经营养因子（GDNF）的新型神经营养因子。 编码GDNF的基因已被克隆并测序。 公开了使用表达人GDNF的可植入细胞或含有表达和分泌神经营养因子的基因工程细胞的生物相容性半透膜的GDNF递送方法和装置。

公开(公告)号：US6015572A

公开(公告)日：2000-01-18

申请号：US453176

申请日：1995-05-30

Applicant: Leu-Fen H. Lin ,  Franklin D. Collins ,  Daniel H. Doherty ,  Jack Lile ,  Susan Bektesh

Inventor： Leu-Fen H. Lin ,  Franklin D. Collins ,  Daniel H. Doherty ,  Jack Lile ,  Susan Bektesh

IPC: A61K38/00 ,  A61K9/00 ,  A61K9/22 ,  A61K31/00 ,  A61K39/395 ,  A61M37/00 ,  A61P25/00 ,  A61P25/16 ,  A61P25/26 ,  A61P25/28 ,  C07H15/12 ,  C07K20060101 ,  C07K1/20 ,  C07K1/22 ,  C07K14/47 ,  C07K14/475 ,  C07K14/52 ,  C07K16/00 ,  C07K16/24 ,  C12N20060101 ,  C12N1/21 ,  C12N1/22 ,  C12N5/02 ,  C12N5/06 ,  C12N5/08 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12P21/06 ,  C12P21/08 ,  C12R1/19 ,  C12R1/91 ,  A61F2/00 ,  C12N11/04

CPC classification number: A61K9/0085 ,  C07K14/475 ,  A61K38/00 ,  Y10S530/809 ,  Y10S530/839

Abstract: A novel neurotrophic factor referred to as glial cell line-derived neurotrophic factor (GDNF) has been identified and isolated from serum free growth medium of B49 glioblastoma cells. Rat and human genes encoding GDNF have been cloned and sequenced. A gene encoding GDNF has been subcloned into a vector, and the vector has been used to transform a host cell in order to produce biologically active GDNF in a recombinant DNA process. An implantable device containing recombinant GDNF secreting cells encapsulated in a semipermeable membrane may be used to treat nerve damage in patients suffering from disorders such as Parkinson's disease.

Abstract translation: 从B49胶质母细胞瘤细胞的无血清生长培养基中鉴定并分离出称为神经胶质细胞系衍生的神经营养因子（GDNF）的新型神经营养因子。 编码GDNF的大鼠和人类基因已被克隆并测序。 编码GDNF的基因已经被亚克隆到载体中，并且该载体已被用于转化宿主细胞，以便在重组DNA过程中产生生物活性的GDNF。 可以使用含有包封在半透膜中的重组GDNF分泌细胞的可植入装置来治疗患有帕金森病等病症的患者的神经损伤。