中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

134 records (took 0.03 seconds)

    Cis/trans riboregulators
    34.
    发明申请
    Cis/trans riboregulators 有权
    顺式/反式核糖调节剂

    公开(公告)号：US20070136827A1

    公开(公告)日：2007-06-14

    申请号：US10535128

    申请日：2003-11-14

    Applicant: James Collins ,  Farren Isaacs ,  Charles Cantor ,  Daniel Dwyer

    Inventor： James Collins ,  Farren Isaacs ,  Charles Cantor ,  Daniel Dwyer

    IPC: A01K67/027 ,  C07H21/04 ,  C12N15/09 ,  C12N5/06

    CPC classification number: C12N15/67 ,  C12N15/11 ,  C12N2310/53 ,  C12Q1/6897

    Abstract: The present invention provides nucleic acid molecules, DNA constructs, plasmids, and methods for post-transcriptional regulation of gene expression using RNA molecules to both repress and activate translation of an open reading frame. Repression of gene expression is achieved through the presence of a regulatory nucleic acid element (the cis-repressive RNA or crRNA) within the 5′ untranslated region (5′ UTR) of an mRNA molecule. The nucleic acid element forms a hairpin (stem/loop) structure through complementary base pairing. The hairpin blocks access to the mRNA transcript by the ribosome, thereby preventing translation. In particular, in embodiments of the invention designed to operate in prokaryotic cells, the stem of the hairpin secondary structure sequesters the ribosome binding site (RBS). In embodiments of the invention designed to operate in eukaryotic cells, the stem of the hairpin is positioned upstream of the start codon, anywhere within the 5′ UTR of an mRNA. A small RNA (trans-activating RNA, or taRNA), expressed in trans, interacts with the crRNA and alters the hairpin structure. This alteration allows the ribosome to gain access to the region of the transcript upstream of the start codon, thereby activating transcription from its previously repressed state.

    Abstract translation: 本发明提供核酸分子，DNA构建体，质粒和用于转录后调节基因表达的方法，其使用RNA分子抑制和激活开放阅读框的翻译。 通过在mRNA分子的5'非翻译区（5'UTR）内存在调节性核酸元件（顺式抑制性RNA或crRNA）来实现基因表达的抑制。 核酸元件通过互补碱基配对形成发夹（茎/环）结构。 发夹阻止核糖体进入mRNA转录物，从而阻止翻译。 特别地，在设计用于在原核细胞中操作的本发明的实施方案中，发夹二级结构的茎螯合核糖体结合位点（RBS）。 在设计用于在真核细胞中操作的本发明的实施方案中，发夹的茎位于起始密码子的上游，位于mRNA的5'UTR内的任何位置。 以反式表达的小RNA（反式激活RNA或taRNA）与crRNA相互作用并改变发夹结构。 这种改变允许核糖体进入起始密码子上游的转录物区域，从而从其先前的抑制状态激活转录。

    Dispenser
    35.
    发明申请
    Dispenser 审中-公开
    分配器

    公开(公告)号：US20070095851A1

    公开(公告)日：2007-05-03

    申请号：US10561404

    申请日：2004-07-09

    Applicant: Gregor Anderson ,  James Collins ,  Ralph Lamble ,  Allen Pearson ,  Tom Snow ,  Paul Turner

    Inventor： Gregor Anderson ,  James Collins ,  Ralph Lamble ,  Allen Pearson ,  Tom Snow ,  Paul Turner

    IPC: B65B59/00

    CPC classification number: B65D83/0409 ,  A61J1/03 ,  A61J7/0076 ,  A61J7/02 ,  A61J7/04 ,  B65D50/041 ,  B65D2583/0481

    Abstract: A hand-held dispenser (1) for dispensing a multiplicity of unit products (3) having a storage area (100) for storing the unit products, an outlet opening (311) through which the unit products are dispensable from the dispenser and a dispensing mechanism (350) adapted to dispense a predetermined number of said unit products through the outlet opening per actuation thereof. The dispenser is adapted such that the dispensing mechanism is actuable by pushing the dispenser into a palm of a user and such that the associated predetermined number of unit products is dispensed into that palm.

    Abstract translation: 一种用于分配具有用于存储单元产品的存储区域（100）的多个单元产品（3）的手持式分配器（1），单元产品通过该出口开口（311）可以从分配器中分出，并且分配 机构（350），其适于通过每个致动的出口开口分配预定数量的所述单元产品。 分配器适于使得分配机构可通过将分配器推入使用者的手掌而致动，并且使得相关联的预定数量的单位产品被分配到该手掌中。

    Electret Condenser Microphone
    36.
    发明申请
    Electret Condenser Microphone 审中-公开
    驻极体冷凝器麦克风

    公开(公告)号：US20070025571A1

    公开(公告)日：2007-02-01

    申请号：US11530192

    申请日：2006-09-08

    Applicant: James Collins

    Inventor： James Collins

    IPC: H04R25/00

    CPC classification number: H04R19/04 ,  H04R1/38 ,  H04R19/016

    Abstract: An electret condenser microphone includes a housing, a diaphragm disposed within the housing, first and second sound inlet ports and an acoustic resistive material disposed within the housing and in the acoustic path between the second inlet port and the diaphragm. The acoustic resistive may further electrically couple a backplate coupled to the diaphragm to an amplifier disposed within the housing.

    Abstract translation: 驻极体电容麦克风包括壳体，设置在壳体内的隔膜，第一和第二声音入口端口以及设置在壳体内以及在第二入口和隔膜之间的声学​​路径中的声阻材料。 声阻可以进一步将耦合到隔膜的背板电耦合到设置在壳体内的放大器。

    Codco low pressure gas driven engine operating system
    38.
    发明申请
    Codco low pressure gas driven engine operating system 审中-公开
    Codco低压气体驱动发动机操作系统

    公开(公告)号：US20060191509A1

    公开(公告)日：2006-08-31

    申请号：US11090603

    申请日：2005-02-28

    Applicant: James Collins

    Inventor： James Collins

    IPC: F02B53/00

    CPC classification number: F01C1/356 ,  F02B53/02

    Abstract: Sometime in 1925 when I was thirteen years old, my older brother and I were sitting on the floor of his garage, putting together a motorcycle to become my means of transportation. Suddenly he said, “Jim, figure out a way to make the pistons go around in a circle.”I would have forgotten all about this, had I not known that he had reworked the single cylinder engine of an Indian Junior Scout motorcycle for his ninety pound step-daughter to ride in the races, in the desert above the City of San Bernardino Calif. She was continually beating the Harley-Davidson motorcycles. The pistons in “The Codco engine” of, “The Codco low pressure gas driven engine operating system,” go around in a circle.

    Abstract translation: 在1925年，当我十三岁的时候，我的哥哥和我坐在他的车库的地板上，把摩托车放在一起成为我的交通工具。 突然他说：“吉姆，想出一个让活塞绕圈子的方法，”我会忘记这一切，如果我不知道他已经为印度少年童军摩托车的单缸发动机 在圣贝尔迪诺市加利福尼亚州上空的沙漠中，九十磅重的女儿骑在比赛中，她一直在殴打哈雷戴维森摩托车。 “Codco低压气体驱动发动机操作系统”的“Codco发动机”中的活塞环绕一圈。

    Fluid distribution system
    40.
    发明申请
    Fluid distribution system 有权
    流体分配系统

    公开(公告)号：US20060113791A1

    公开(公告)日：2006-06-01

    申请号：US11328699

    申请日：2006-01-10

    Applicant: Gunter Matt ,  James Collins ,  Michael Cain ,  Paul Adkins

    Inventor： Gunter Matt ,  James Collins ,  Michael Cain ,  Paul Adkins

    IPC: F16L41/08

    CPC classification number: F16L21/08 ,  F16L25/0009

    Abstract: An outlet plate fitting configured for attaching a secondary component to a fluid pipe section. The fluid pipe section includes opposed flanges that define a receiving groove having a bottom surface. The outlet plate fitting includes a main body, an engagement platform connected to and at a distance from the main body, and a bore extending through the main body and the engagement platform. A first surface of the engagement platform faces away from the main body and is a planar surface with a seal member positioned about the bore. A second surface of the engagement platform faces toward the main body and has a ramped surface, such that rotation of the engagement platform in a first direction within the receiving groove causes the planar surface and seal member into sealing engagement with the bottom surface of the receiving groove.

    Abstract translation: 出口板配件，其构造成用于将次要部件附接到流体管段。 流体管部分包括限定具有底表面的接收槽的相对的凸缘。 出口板配件包括主体，与主体连接并且距离主体一定距离的接合平台，以及延伸穿过主体和接合平台的孔。 接合平台的第一表面远离主体并且是具有围绕孔定位的密封构件的平坦表面。 接合平台的第二表面面向主体并且具有倾斜表面，使得接合平台沿接收槽内的第一方向的旋转使得平面表面和密封构件与接收器的底表面密封接合 槽。

Patent Agency Ranking