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    Multiply-Substituted Protease Variants
    37.
    发明申请
    Multiply-Substituted Protease Variants 审中-公开
    多重取代的蛋白酶变体

    公开(公告)号：US20110086412A1

    公开(公告)日：2011-04-14

    申请号：US12776932

    申请日：2010-05-10

    Applicant: Ayrookaran J. Poulose ,  David A. Estell ,  James T. Kellis, JR. ,  Richard R. Bott

    Inventor： Ayrookaran J. Poulose ,  David A. Estell ,  James T. Kellis, JR. ,  Richard R. Bott

    IPC: C12N9/50 ,  C12N15/57 ,  C12N15/63 ,  C12N1/00

    CPC classification number: C12N9/54 ,  C11D3/386 ,  C11D3/38609 ,  C12Y304/21062

    Abstract: Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 of Bacillus amyloliquefaciens subtilisin. Additional variants comprising at least one additional substitution at a position selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 of Bacillus amyloliquefaciens subtilisin are also described.

    Abstract translation: 公开了包含衍生自天然或重组非人蛋白酶的DNA序列的蛋白酶变体的新型酶变体。 一般来说，变体蛋白酶通过体外修饰编码天然存在或重组蛋白酶的前体DNA序列获得，以产生前体蛋白酶的氨基酸序列中的多个氨基酸残基的取代。 这种变体蛋白酶具有与前体蛋白酶不同的性质，例如改变的洗涤性能。 取代的氨基酸残基对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置27,45,170,181,251和271。 还描述了包含在解淀粉芽孢杆菌枯草杆菌蛋白酶的1,4,14,69,61,87,100,102,118,128,204和258的位置上的至少一个另外的取代的其它变体。

    Multiply-substituted protease variants
    39.
    发明授权
    Multiply-substituted protease variants 有权
    多重取代的蛋白酶变体

    公开(公告)号：US07306937B2

    公开(公告)日：2007-12-11

    申请号：US10500943

    申请日：2003-01-16

    Applicant: Ayrookaran J. Poulose ,  David A Estell ,  James T Kellis, Jr. ,  Richard R. Bott

    Inventor： Ayrookaran J. Poulose ,  David A Estell ,  James T Kellis, Jr. ,  Richard R. Bott

    IPC: C12N9/52 ,  C12N9/54 ,  C12N15/57 ,  C12N15/74 ,  C11D3/386

    CPC classification number: C12N9/54 ,  C11D3/386 ,  C11D3/38609 ,  C12Y304/21062

    Abstract: Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or re-combinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 of Bacillus amyloliquefaciens subtilisin. Additional variants comprising at least one additional substitution at a position selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 of Bacillus amyloliquefaciens subtilisin are also described.

    Abstract translation: 公开了包含衍生自天然存在或重组非人蛋白酶的DNA序列的蛋白酶变体的新型酶变体。 一般来说，变体蛋白酶通过体外修饰编码天然存在或重组蛋白酶的前体DNA序列获得，以产生前体蛋白酶的氨基酸序列中的多个氨基酸残基的取代。 这种变体蛋白酶具有与前体蛋白酶不同的性质，例如改变的洗涤性能。 取代的氨基酸残基对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置27,45,170,181,251和271。 还描述了包含在解淀粉芽孢杆菌枯草杆菌蛋白酶的1,4,14,69,61,87,100,102,118,128,204和258的位置上的至少一个另外的取代的其它变体。

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