公开(公告)号：US07655792B2

公开(公告)日：2010-02-02

申请号：US11857468

申请日：2007-09-19

Applicant: Sudhakar Rao Takkellapati ,  Rajesh Ambat

Inventor： Sudhakar Rao Takkellapati ,  Rajesh Ambat

IPC: C07H21/00

CPC classification number: C12N15/101

Abstract: The invention provides an improved method for the purification of nucleic acid molecules, which method comprises generating a cellular lysate containing the nucleic acid; contacting the lysate with an anion exchanger bound to a solid support matrix under conditions such that the anion exchanger binds the nucleic acid; followed by eluting the nucleic acid from the anion exchanger with an aqueous mobile phase comprising an elution solution; and desalting the eluted nucleic acid such that it is suitable for downstream applications. The improvement of the method includes adding in the elution solution a composition such that the pH of the aqueous mobile phase is between about pH 9 and about pH 13, wherein the presence of the composition in the elution solution provides an increase in nucleic acid recovery, as compared with the recovery in the absence of the composition.

Abstract translation: 本发明提供了一种用于纯化核酸分子的改进方法，该方法包括产生含有核酸的细胞裂解物; 使所述裂解物与阴离子交换剂接触，所述阴离子交换剂在使所述阴离子交换剂结合所述核酸的条件下与固体支持基质结合; 随后用含有洗脱溶液的水性流动相从阴离子交换剂洗脱核酸; 并使洗脱的核酸脱盐，使其适合于下游应用。 该方法的改进包括在洗脱溶液中加入组合物使得水性流动相的pH在约pH9至约pH13之间，其中组合物在洗脱溶液中的存在提供了核酸回收率的增加， 与没有组合物的恢复相比。

公开(公告)号：US07514463B2

公开(公告)日：2009-04-07

申请号：US10922747

申请日：2004-08-20

Applicant: Gunda I. Georg ,  Joseph S. Tash ,  Ramappa Chakrasali ,  Sudhakar Rao Jakkaraj

Inventor： Gunda I. Georg ,  Joseph S. Tash ,  Ramappa Chakrasali ,  Sudhakar Rao Jakkaraj

IPC: A61K31/416 ,  C07D231/56

CPC classification number: C07D231/56 ,  C07D471/04

Abstract: Novel compounds useful for inhibiting spermatogenesis and cancer treatment, and in particular as inhibitors of heat shock proteins and/or elongation factor 1 alpha.

Abstract translation: 用于抑制精子发生和癌症治疗的新型化合物，特别是作为热休克蛋白和/或延伸因子1α的抑制剂。

公开(公告)号：US07463207B1

公开(公告)日：2008-12-09

申请号：US11594157

申请日：2006-11-08

Applicant: Sudhakar Rao ,  Mihn Tang

Inventor： Sudhakar Rao ,  Mihn Tang

IPC: H01Q13/00

CPC classification number: H01Q13/0208 ,  H01Q13/0266 ,  H01Q19/17

Abstract: A multiple-beam antenna system includes at least one reflector and a cluster of horns for feeding the reflector. A horn of the cluster of horns is configured for providing transmission and reception of signals over respective transmission and reception frequency bands. The horn includes a substantially conical wall having an internal surface with a variable slope. The internal surface of the substantially conical wall includes slope discontinuities. At least one of the slope discontinuities has a diameter greater than 1.7 times the wavelength of the lowest frequency of the transmission frequency band. The diameter is also greater than 1.7 times the wavelength of the highest frequency of the transmission frequency band. In addition, the diameter is greater than 1.7 times the wavelength of the lowest frequency of the reception frequency band, and the diameter is greater than 1.7 times the wavelength of the highest frequency of the reception frequency band. This configuration of the slope discontinuity generates one or more higher order modes of a transverse electric (TE) mode over the transmission and reception frequency bands without generating a transverse magnetic (TM) mode.

Abstract translation: 多波束天线系统包括至少一个反射器和用于馈送反射器的喇叭簇。 喇叭簇的喇叭被配置用于在各个发射和接收频带上提供信号的发送和接收。 喇叭包括具有可变斜率的内表面的基本上圆锥形的壁。 基本圆锥形壁的内表面包括斜率不连续性。 斜率不连续性中的至少一个具有大于传输频带最低频率波长的1.7倍的直径。 直径也大于传输频带最高频率波长的1.7倍。 此外，直径大于接收频带最低频率波长的1.7倍，直径大于接收频带最高频率波长的1.7倍。 倾斜不连续的这种配置在发射和接收频带上产生横向电（TE）模式的一个或多个更高阶模式，而不产生横向磁（TM）模式。

公开(公告)号：US20080076911A1

公开(公告)日：2008-03-27

申请号：US11857727

申请日：2007-09-19

Applicant: SIBYLLE HERZER ,  SUDHAKAR RAO TAKKELLAPATI ,  RAJESH AMBAT

Inventor： SIBYLLE HERZER ,  SUDHAKAR RAO TAKKELLAPATI ,  RAJESH AMBAT

IPC: C07H21/00

CPC classification number: C12N15/101

Abstract: Disclosed is a process for separating and/or purifying a nucleic acid by elution of the nucleic acid from anion exchange resins under conditions of high salt concentration and the presence in the eluant of an additive comprising guanidine, or a guanidine-like derivative. The process allows high recovery of nucleic acids from anion exchange resins without impairing the nucleic acid stability as compared with conventional ion exchange chromatographic procedures.

Abstract translation: 公开了一种通过在高盐浓度的条件下从阴离子交换树脂中洗脱核酸和在包含胍或类胍衍生物的添加剂的洗脱液中的存在来分离和/或纯化核酸的方法。 与常规离子交换色谱法相比，该方法允许从阴离子交换树脂高度回收核酸而不损害核酸稳定性。

公开(公告)号：US20050052333A1

公开(公告)日：2005-03-10

申请号：US10659826

申请日：2003-09-10

Applicant: Sudhakar Rao ,  David Bressler

Inventor： Sudhakar Rao ,  David Bressler

IPC: H01Q5/00 ,  H01Q5/45 ,  H01Q19/12 ,  H01Q19/17 ,  H01Q25/00

CPC classification number: H01Q25/007 ,  H01Q5/45 ,  H01Q19/17

Abstract: An antenna system includes a reflector having a modified-paraboloid shape; and a multi-beam, multi-band feed array located at a focal point of the reflector so that the antenna system forms a multiple congruent beams that are contiguous. The system has a single reflector with non-frequency selective surface. The reflector is sized to produce a required beam size at K-band frequencies and is oversized at EHF-band frequencies. The synthesized reflector surface is moderately shaped and disproportionately broadens EHF-band and Ka-band beams compared to K-band beams. The synthesized reflector surface forms multiple beams each having a 0.5-degree diameter at K-band, Ka-band, and EHF band. The multi-beam, multi-band feed array includes a number of high-efficiency, multi-mode circular horns that operate in focused mode at K-band and defocused mode at Ka-band and EHF-band by employing “frequency-dependent” design for the horns.

Abstract translation: 天线系统包括具有改性抛物面形状的反射器; 以及位于反射器的焦点处的多光束，多波段馈电阵列，使得天线系统形成连续的多个一致的光束。 该系统具有一个具有非频率选择性表面的反射器。 反射器的尺寸被设计成在K波段频率处产生所需的波束尺寸，并且在EHF频带频率处变大。 合成的反射器表面适度成形，与K波段光束相比，EHF带和Ka波段光束不均衡地扩大。 合成的反射器表面形成多个光束，每个光束在K波段，Ka波段和EHF波段具有0.5度直径。 多波束多频馈电阵列包括多个高效多模环形喇叭，它们在Ka波段和EHF波段的K波段和散焦模式下以聚焦模式工作，采用“频率依赖” 喇叭设计。

公开(公告)号：US06855503B2

公开(公告)日：2005-02-15

申请号：US10326039

申请日：2002-12-20

Applicant: Satyam Nampalli ,  Weihong Zhang ,  Sudhakar Rao ,  Shiv Kumar

Inventor： Satyam Nampalli ,  Weihong Zhang ,  Sudhakar Rao ,  Shiv Kumar

IPC: C09B11/08 ,  C09B11/24 ,  C09B23/08 ,  C12Q1/68 ,  C07D1/02

CPC classification number: C09B23/083 ,  C09B11/08 ,  C09B11/24 ,  C12Q1/6818 ,  C12Q1/6869

Abstract: Exploitation of suitably functionalized heterocyclic molecules, in the design and synthesis of Fluorescence Resonance Energy Transfer (FRET) cassettes and their corresponding dideoxynucleotide terminators culminated into efficient reagents for DNA sequencing. Additionally, these FRET cassettes/terminators, of the present invention, derived from different classes of heterocyclic systems have high potential to be used for general labelling of biological molecules to generate highly sensitized signals. Their preparation, energy transfer efficiency, and use as labels, specifically, in DNA sequencing reactions is disclosed.

Abstract translation: 在荧光共振能量转移（FRET）盒及其相应的双脱氧核苷酸终止子的设计和合成中，适当官能化的杂环分子的开发最终成为DNA测序的有效试剂。 此外，衍生自不同种类的杂环体系的本发明的这些FRET盒/终止子具有很高的潜力用于生物分子的一般标记以产生高致敏信号。 公开了它们的制备，能量转移效率和用作标记，特别是在DNA测序反应中。

公开(公告)号：US08362031B2

公开(公告)日：2013-01-29

申请号：US12395521

申请日：2009-02-27

Applicant: Ingrid Gunda Georg ,  Joseph S. Tash ,  Ramappa Chakrasali ,  Sudhakar Rao Jakkaraj ,  James P. Calvet

Inventor： Ingrid Gunda Georg ,  Joseph S. Tash ,  Ramappa Chakrasali ,  Sudhakar Rao Jakkaraj ,  James P. Calvet

IPC: A61K31/4162 ,  A61K31/416 ,  A61P13/12

CPC classification number: C07D231/56 ,  C07D471/04

Abstract: Lonidamine derivatives can be useful in methods of treating, inhibiting, and/or preventing polycystic kidney disease (PKD). Accordingly, lonidamine derivatives can be administered in a therapeutically effective amount for inhibiting, and/or preventing polycystic kidney disease (PKD) in the subject. This can include administering a therapeutically effective amount of the lonidamine derivatives for inhibiting CFTR and/or Hsp90 or biological pathway thereof. Also, the method can include administering the lonidamine derivatives in a therapeutically effective amount for inhibiting ErbB2, Src, Raf-1, B-Raf, MEK, Cdk4, NKCC1, or combinations thereof. For example, the therapeutically effective amount of the lonidamine derivatives can be configured so as to provide a concentration in or adjacent to a kidney cell of about 0.25 uM or more or less.

Abstract translation: 兰尼达胺衍生物可用于治疗，抑制和/或预防多囊肾病（PKD）的方法。 因此，可以以治疗有效量的方式施用孤啡酸衍生物以抑制和/或预防受试者中的多囊肾病（PKD）。 这可以包括施用治疗有效量的用于抑制CFTR和/或Hsp90或其生物学途径的洋芋胺衍生物。 此外，该方法可以包括以治疗有效量施用用于抑制ErbB2，Src，Raf-1，B-Raf，MEK，Cdk4，NKCC1或其组合的兰酸胺衍生物。 例如，可以配置兰定胺衍生物的治疗有效量，以使其在肾细胞中或邻近肾细胞中的浓度为约0.25uM或更大或更小。

公开(公告)号：US20120071641A1

公开(公告)日：2012-03-22

申请号：US13203927

申请日：2010-03-02

Applicant: Muthiah Manoharan ,  Kallanthottathil G. Rajeev ,  Alexander V. Kelin ,  Sudhakar Rao Takkellapati ,  Shigeo Matsuda

Inventor： Muthiah Manoharan ,  Kallanthottathil G. Rajeev ,  Alexander V. Kelin ,  Sudhakar Rao Takkellapati ,  Shigeo Matsuda

IPC: C07H21/02 ,  C07H19/167 ,  C07H19/10 ,  C07H19/067 ,  C07H21/00 ,  C07H19/20

CPC classification number: C12N15/113 ,  C07H19/073 ,  C07H19/10 ,  C07H19/173 ,  C07H19/20 ,  C07H21/00 ,  C07H21/02 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/334 ,  C12N2310/335 ,  C12N2310/336

Abstract: The present invention provides nucleosides of formula (1) and oligonucleotides comprising at least one nucleoside of formula (2): Another aspect of the invention relates to a method of inhibiting the expression of a gene in cell, the method comprising (a) contacting an oligonucleotide of the invention with the cell; and (b) maintaining the cell from step (a) for a time sufficient to obtain degradation of the mRNA of the target gene.

Abstract translation: 本发明提供式（1）的核苷和包含至少一种式（2）的核苷的寡核苷酸：本发明的另一方面涉及抑制细胞中基因表达的方法，所述方法包括（a）使 本发明的寡核苷酸与细胞; 和（b）将来自步骤（a）的细胞维持足以获得靶基因的mRNA降解的时间。

公开(公告)号：US20110060003A1

公开(公告)日：2011-03-10

申请号：US12830681

申请日：2010-07-06

Applicant: Ingrid Gunda Georg ,  Joseph S. Tash ,  Ramappa Chakrasali ,  Sudhakar Rao Jakkaraj ,  Katherine Roby

Inventor： Ingrid Gunda Georg ,  Joseph S. Tash ,  Ramappa Chakrasali ,  Sudhakar Rao Jakkaraj ,  Katherine Roby

IPC: A61K31/416 ,  A61K31/437 ,  A61P3/00 ,  A61P15/00

CPC classification number: C07D471/04 ,  A61K31/416 ,  A61K31/4162 ,  C07D231/56

Abstract: Fertility management can include: administering to the subject one or more doses of a compound according to Formula I so as to reduce fertility in the subject. Fertility management can also include administering an effective amount of the compound to: impair Sertoli cell function in a male subject; inhibit spermatogenesis in the subject; reduce testis weight in the subject; reduce ovary weight in a female subject; reduce serum progesterone in the female subject; impair ovarian follicle function in the female subject; causing reversible fertility in the subject. In order to return fertility, the method can include ceasing administration of the compound to the subject so as to return fertility in the subject. The compound can be administered for irreversibly sterilizing the subject.

Abstract translation: 生育能力管理可以包括：向受试者施用一种或多种剂量的式I化合物，以降低受试者的生育能力。 生育能力管理还可以包括给予有效量的化合物以损害男性受试者的Sertoli细胞功能; 抑制受试者的精子发生; 减少受试者的睾丸重量; 减少女性受试者的卵巢重量; 减少女性受试者的血清孕酮; 影响女性卵巢卵泡功能; 导致受试者的可逆生育力。 为了返回生育力，该方法可以包括停止对受试者施用化合物以使受试者的生育力恢复。 该化合物可被用于不可逆地灭菌受试者。

公开(公告)号：US07490473B2

公开(公告)日：2009-02-17

申请号：US11094908

申请日：2005-03-31

Applicant: Vinay Bhaskar Jammu ,  Abhay Sudhakar Rao Kant ,  Krishna Vardhan Pullatikurti

Inventor： Vinay Bhaskar Jammu ,  Abhay Sudhakar Rao Kant ,  Krishna Vardhan Pullatikurti

IPC: F25B9/00

CPC classification number: G01R33/3815 ,  F25B9/14 ,  F25B49/005 ,  F25D29/001

Abstract: The method includes obtaining input data representative of a first parameter of a cryogenic medium in a cryogenic cooling system is provided. The method further includes calculating an expected value representative of a second parameter of the cryogenic medium in the cooling system via a predefined physics based model. A second input data representative of the second parameter of the cryogenic medium in the cooling system is estimated. The expected value is compared to the second input data and difference between the expected value and the second input data is correlated to statistical data to estimate a likelihood of cryogenic cooling system event.

Abstract translation: 该方法包括获得表示低温冷却系统中的低温介质的第一参数的输入数据。 该方法还包括经由预定义的基于物理的模型来计算代表冷却系统中的低温介质的第二参数的期望值。 估计代表冷却系统中的低温介质的第二参数的第二输入数据。 将期望值与第二输入数据进行比较，并且期望值和第二输入数据之间的差异与统计数据相关以估计低温冷却系统事件的可能性。