摘要:
A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3'-5' exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.
摘要:
A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3'-5' exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.
摘要:
A modified thermostable DNA polymerase having 5% or less of the 3'-5' exonuclease activity of the enzyme before modification and a DNA polymerase composition for amplifying nucleic acid, which comprises the modified thermostable DNA polymerase having 0 to 5% of the 3'-5' exonuclease activity of the enzyme before modification and an unmodified thermostable DNA polymerase having 3'-5' exonuclease activity or a modified thermostable DNA polymerase having 100 to 6% of the 3'-5' exonuclease activity of a thermostable DNA polymerase before modification; a method for amplifying nucleic acid by use of said modified thermostable polymerase or said DNA polymerase composition; and a reagent therefor.
摘要:
An air/fuel ratio control system detects transition of air/fuel ratio changing between rich and lean for checking if updating condition is satisfied. Updating value is derived based on a feedback correction value which is derived for adjusting air/fuel ratio toward a stoichiometric value. With the updating value thus derived, one of a plurality of learnt correction value which are set with respect to various engine driving range.
摘要:
A basic pulse width T.sub.p is determined in accordance with the throttle valve opening and speed of an engine, and the basic pulse width T.sub.p is corrected when the peak value of cylinder pressures exceeds a given range established by the average value of the peak pressure values.
摘要:
A measurement device for measuring a physical property such as temperature difference, displacement, pressure, fluid flow rate, etc. comprises: (a) an amplifying means which receives and amplifies a voltage signal corresponding to a detected physical property; (b) a reference voltage generator which generates and applies a reference voltage to the amplifying means at a predetermined time; (c) a memory which stores the output voltage signal from the amplifying means when the reference voltage generator applies the reference voltage to the amplifying means; and (d) a calculating means which outputs at least one command signal to the reference voltage generator to apply the reference voltage to the amplifying means and to the memory to store the output voltage of the amplifying means into a specified memory location thereof at the predetermined time and calculates the value of the desired physical property from the current output voltage of the amplifying means with reference to the stored voltage value in the memory, whereby an accurate physical property measurement can be made by compensating for individual differences and deterioration of the characteristics of the measurement device.
摘要:
The present invention makes it possible that in a magnetic recording medium having a magnetic layer which contains therein ferromagnetic powder and serving as a magnetic recording medium for short wavelength recording in, for example, a video tape recorder, when the coercive force of the magnetic layer is over 1000 Oe, the specific surface area of its ferromagnetic alloy powder measured according to BET adsorption method is made 45 m.sup.2 /g or above, and the high density recording where the central recording wavelength is not more than 1 .mu.m is carried out thus recording and playback with low noise and high output being made.
摘要:
Fuel is periodically injected into an engine combustion chamber by a first injection device as an engine crankshaft rotates. A timing control device serves to adjust the timing of fuel injection effected by the first injection device with respect to the rotational angle of the crankshaft. Fuel is also periodically injected into the combustion chamber by a second injection device as the crankshaft rotates. Adjustment of the timing of fuel injection effected by the first injection device results in variation of the total fuel injection rate characteristic curve with respect to the rotational angle of the crankshaft.
摘要:
An exhaust gas recirculation system is applied to an internal combustion engine having a combustion chamber and an exhaust passage conducting exhaust gas from the combustion chamber to the atmosphere. The system includes a first mechanism provided to recirculate the exhaust gas through the combustion chamber. A second mechanism is associated with the first mechanism to adjustably determine the percentage of the exhaust gas recirculated. A sensor detects the average pressure in the exhaust passage. In response to the detected exhaust pressure, a third mechanism controls the second mechanism in such a manner that the percentage of the exhaust gas recirculated is independent of the variations in the average pressure in the exhaust passage.
摘要:
An E.G.R. control system for a diesel engine is disclosed wherein a deterioration in combustion is avoided upon depressing an accelerator pedal for reacceleration after the momentary release of the accelerator pedal. An E.G.R. control unit is responsive to a load indicative signal from a load sensor and a revolution signal from a revolution sensor and generates an engine operating state indicative signal indicating that the engine operates in low load state which requires high E.G.R. flow rate. A monostable circuit, a delay circuit and a logic circuit make up a circuit means for preventing a throttle valve from being closed until the engine keeps operating in the low load state for a time longer than a predetermined time.