公开(公告)号：US20190176152A1

公开(公告)日：2019-06-13

申请号：US16325922

申请日：2017-08-23

Applicant: 10X Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Tobias Daniel Wheeler ,  Kevin Ness ,  Benjamin Hindson

IPC: B01L3/00 ,  B01F13/00 ,  B01F3/08

CPC classification number: B01L3/502784 ,  B01F3/0807 ,  B01F3/0811 ,  B01F13/0086 ,  B01F2215/0037 ,  B01J2/06 ,  B01L2200/0673 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/16 ,  C12Q1/6844 ,  C12Q2531/113 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.

公开(公告)号：US12264316B2

公开(公告)日：2025-04-01

申请号：US16419428

申请日：2019-05-22

Applicant: 10X Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Michael Schnall-Levin ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Pranav Patel ,  Katherine Pfeiffer ,  Andrew D. Price ,  Mohammad Rahimi Lenji ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US12084716B2

公开(公告)日：2024-09-10

申请号：US17473760

申请日：2021-09-13

Applicant: 10X Genomics, Inc.

Inventor： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo ,  Stephane Claude Boutet ,  Sarah Taylor ,  Niranjan Srinivas

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6874

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/179

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20240210161A1

公开(公告)日：2024-06-27

申请号：US18390503

申请日：2023-12-20

Applicant: 10x Genomics, Inc.

Inventor： Tobias Daniel Wheeler ,  Augusto Manuel Tentori

IPC: G01B11/06

CPC classification number: G01B11/0608

Abstract: A method includes receiving an image of a plurality of fiducial markers, where the plurality of fiducial markers has at least one predetermined height relative to a substrate. The method also includes determining at least one optical metric associated with the plurality of fiducial markers within the image, and determining a calibration model based on the at least one predetermined height and the at least one optical metric.

公开(公告)号：US20230278037A1

公开(公告)日：2023-09-07

申请号：US18177504

申请日：2023-03-02

Applicant: 10x Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Lynna Chen ,  Francis Cui ,  Daniel Freitas ,  Mohammad Rahimi Lenji ,  Martin Sauzade ,  Augusto Manuel Tentori ,  Tobias Daniel Wheeler

IPC: B01L3/00

CPC classification number: B01L3/502784 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/0851 ,  B01L2300/0858 ,  B01L2300/0864 ,  B01L2300/0867

Abstract: Devices, systems, and their methods of use, for generating and collecting droplets are provided. The invention provides multiplex devices that increase droplet formation in a limited area.

公开(公告)号：US11459607B1

公开(公告)日：2022-10-04

申请号：US16708214

申请日：2019-12-09

Applicant: 10X GENOMICS, INC.

Inventor： Jessica Michele Terry ,  Tobias Daniel Wheeler

IPC: C12P19/34 ,  C12Q1/6869 ,  C12Q1/6834

Abstract: Provided herein are methods, compositions, and systems for multiplexed analysis of individual cells or cell populations. Cells encapsulated in beads and/or biomolecules are sequentially co-partitioned, allowing for analysis of two different types of biomolecules (e.g., RNA and DNA). The present invention leverages different polymer dissociation mechanisms, accompanied with barcoding of biomolecules (e.g., nucleic acid molecules) for multiplexed measurements in single cells. Sequential co-partitioning and barcode technology enables identification and quantitation of DNA and RNA from single cells.

公开(公告)号：US11193122B2

公开(公告)日：2021-12-07

申请号：US17208369

申请日：2021-03-22

Applicant: 10X Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Michael Schnall-Levin ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Pranav Patel ,  Andrew D. Price ,  Mohammad Rahimi Lenji ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US11084036B2

公开(公告)日：2021-08-10

申请号：US16180356

申请日：2018-11-05

Applicant: 10X GENOMICS, INC.

Inventor： Rajiv Bharadwaj ,  Kevin Ness ,  Tobias Daniel Wheeler

IPC: B01L3/00 ,  G01N1/28 ,  G01N33/487

Abstract: Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.

公开(公告)号：US20210222158A1

公开(公告)日：2021-07-22

申请号：US17208369

申请日：2021-03-22

Applicant: 10X Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Michael Schnall-Levin ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Pranav Patel ,  Katherine Pfeiffer ,  Andrew D. Price ,  Mohammad Rahimi Lenji ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US20200002763A1

公开(公告)日：2020-01-02

申请号：US16439675

申请日：2019-06-12

Applicant: 10X Genomics, Inc.

Inventor： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo ,  Stephane Claude Boutet ,  Sarah Taylor ,  Niranjan Srinivas

IPC: C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6806

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.