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    Lipid acyltransferases
    31.
    发明授权
    Lipid acyltransferases 有权
    脂质酰基转移酶

    公开(公告)号：US08030044B2

    公开(公告)日：2011-10-04

    申请号：US12040721

    申请日：2008-02-29

    申请人： Jørn Borch Søe ,  Jørn Dalgaard Mikkelson ,  Arno de Kreij

    发明人： Jørn Borch Søe ,  Jørn Dalgaard Mikkelson ,  Arno de Kreij

    IPC分类号： C12N9/10 ,  C07H21/04

    CPC分类号： C12N9/1029 ,  A21D8/042 ,  A23L15/25 ,  A23L33/10 ,  A23L33/195 ,  C11B3/003 ,  C12P7/62 ,  C12P7/6436 ,  C12P7/6454 ,  C12P7/6481

    摘要： The present invention relates to a method of producing a variant lipid acyltransferase enzyme by selecting a parent enzyme which is a lipid acyltransferase enzyme having the amino acid sequence motif GDSX, modifying one or more amino acids to produce a variant lipid acyltransferase, testing the variant lipid acyltransferase for activity on a galactolipid substrate, a phospholipid substrate and/or a triglyceride substrate, selecting a variant enzyme with an enhanced activity towards galactolipids compared with the parent enzyme, and/or preparing a quantity of the variant enzyme. In some embodiments, the variant lipid acyltransferase enzyme may include the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, X, Q, T N, M or S, and wherein the variant enzyme has one or more amino acid modifications compared with a parent sequence.

    摘要翻译： 本发明涉及通过选择具有氨基酸序列基序GDSX的脂质酰基转移酶的母体酶，修饰一个或多个氨基酸以产生变体脂质酰基转移酶来生产变体脂质酰基转移酶的方法，测试变体脂质 用于在半乳糖脂底物，磷脂底物和/或甘油三酯底物上的活性的酰基转移酶，与母体酶相比，选择具有增强的对乳糖脂的活性的变体酶和/或制备一定量的变体酶。 在一些实施方案中，变体脂质酰基转移酶可以包括氨基酸序列基序GDSX，其中X是以下氨基酸残基L，A，V，I，F，Y，X，Q，TN，M中的一个或多个或 S，并且其中所述变体酶与亲本序列相比具有一个或多个氨基酸修饰。

    PROCESS
    32.
    发明申请
    PROCESS 有权
    处理

    公开(公告)号：US20100215803A1

    公开(公告)日：2010-08-26

    申请号：US12706210

    申请日：2010-02-16

    申请人： Niels Erik Larsen ,  Jørn Borch Søe

    发明人： Niels Erik Larsen ,  Jørn Borch Søe

    IPC分类号： A23C9/12 ,  A23C9/123 ,  A23C3/02

    CPC分类号： A21D8/042 ,  A23C19/0328 ,  A23C19/0684 ,  A23L15/25 ,  A23L29/06 ,  C12N9/1029 ,  C12N9/18 ,  C12N11/00 ,  C12Y301/01

    摘要： The use of a lipid acyltransferase in the manufacture of UHT milk for improving the stability, particularly the long term stability, and/or improving the perceptible sensory difference and/or improving smell and/or taste and/or for reducing the cholesterol content and/or for eliminating or reducing creaming of the UHT milk for reducing the cholesterol content in the UHT milk. A method of producing UHT milk, wherein method comprises admixing a lipid acyltransferase and milk (including a step of processing the milk to make it a UHT milk). Preferably said lipid acyltranferase is a polypeptide having lipid acyltransferase activity which polypeptide is obtained by expression of the nucleotide sequence shown as SEQ ID No. 49 or a nucleotide sequence which as has 70% or more identity therewith; and/or is obtained by expression of a nucleic acid which hybridises under medium stringency conditions to a nucleic probe comprising the nucleotide sequence shown as SEQ ID No. 49; and/or is a polypeptide having lipid acyltranferase activity which polypeptide comprises the amino acid sequence shown as SEQ ID No. 68 or an amino acid sequence which as has 70% or more identity therewith.

    摘要翻译： 脂质酰基转移酶在制造UHT乳中用于改善稳定性，特别是长期稳定性和/或改善可察觉的感觉差异和/或改善气味和/或味道和/或降低胆固醇含量和/ 或用于消除或减少UHT牛奶的奶油以降低UHT牛奶中的胆固醇含量。 一种生产UHT奶的方法，其中方法包括混合脂质酰基转移酶和乳（包括加工牛奶以使其成为UHT牛奶的步骤）。 优选地，所述脂质酰基转移酶是具有脂质酰基转移酶活性的多肽，其多肽通过表达如SEQ ID No.49所示的核苷酸序列或与其同一性具有70％或更多同一性的核苷酸序列获得; 和/或通过在中等严格条件下与包含SEQ ID No.49所示的核苷酸序列的核酸探针杂交的核酸的表达获得; 和/或是具有脂质酰基转移酶活性的多肽，该多肽包含SEQ ID No.68所示的氨基酸序列或与其同一性具有70％以上的氨基酸序列。

    METHOD
    37.
    发明申请
    METHOD 审中-公开
    方法

    公开(公告)号：US20120231116A1

    公开(公告)日：2012-09-13

    申请号：US13510499

    申请日：2010-11-08

    申请人： Niels Erik Larsen ,  Jørn Borch Søe

    发明人： Niels Erik Larsen ,  Jørn Borch Søe

    IPC分类号： A23C9/18

    CPC分类号： A23C9/1216 ,  C07K14/71 ,  C12Y203/01043

    摘要： A method of producing powder milk, said method comprising: (a) contacting milk or a fraction thereof with a lipid acyltransferase enzyme; and (b) drying the enzyme treated milk to produce powder milk; is disclosed. Powder milk products produced by the method and use of a lipid acyltransferase in the manufacture of powder milk for improving the rehydration properties, the perceptible sensory difference (smell and/or taste) and the flowability of the powder milk, and for reducing the cholesterol content and/or the free fatty acid content of the powder milk, and for reducing fouling of the equipment used in the manufacture of the powder milk, are also disclosed.

    摘要翻译： 一种生产粉末乳的方法，所述方法包括：（a）使乳或其一部分与脂质酰基转移酶接触; 和（b）干燥酶处理的牛奶以产生粉末奶; 被披露。 通过脂质酰基转移酶的制造方法生产的粉末乳制品，用于制造粉末乳，用于改善补液性能，可感知的感觉差异（气味和/或味道）和粉末奶的流动性以及降低胆固醇含量 和/或粉末奶的游离脂肪酸含量，以及用于减少用于制造粉末乳的设备的结垢。