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    Method for producing ultra-heat treatment milk
    1.
    发明授权
    Method for producing ultra-heat treatment milk 有权
    生产超热处理牛奶的方法

    公开(公告)号：US08652809B2

    公开(公告)日：2014-02-18

    申请号：US12706210

    申请日：2010-02-16

    申请人： Niels Erik Larsen ,  Jørn Borch Søe

    发明人： Niels Erik Larsen ,  Jørn Borch Søe

    IPC分类号： C12P19/58

    CPC分类号： A21D8/042 ,  A23C19/0328 ,  A23C19/0684 ,  A23L15/25 ,  A23L29/06 ,  C12N9/1029 ,  C12N9/18 ,  C12N11/00 ,  C12Y301/01

    摘要： The invention relates to using a lipid acyltransferase polypeptide (SEQ ID NO: 68) in the manufacture of ultra-heat treatment milk (UHT) for improving the stability, the perceptible sensory difference, the smell, and the taste; and for reducing the cholesterol content, and for eliminating or reducing creaming of the UHT milk. The invention also relates to a method of producing UHT milk, wherein the method comprises admixing a lipid acyltransferase with milk and heating the mixture to make it a UHT milk.

    摘要翻译： 本发明涉及使用脂质酰基转移酶多肽（SEQ ID NO：68）制造超热处理牛奶（UHT），用于改善稳定性，可察觉的感觉差异，气味和味道; 并且用于降低胆固醇含量，以及用于消除或减少UHT牛奶的奶油。 本发明还涉及一种生产UHT乳的方法，其中所述方法包括将脂质酰基转移酶与乳混合并加热混合物以使其成为UHT牛奶。

    METHOD FOR PRODUCING PHYTOSTEROL/PHYTOSTANOL PHOSPHOLIPID ESTERS
    3.
    发明申请
    METHOD FOR PRODUCING PHYTOSTEROL/PHYTOSTANOL PHOSPHOLIPID ESTERS 审中-公开
    用于生产植物甾醇/植物甾醇磷脂酯的方法

    公开(公告)号：US20120142644A1

    公开(公告)日：2012-06-07

    申请号：US13231355

    申请日：2011-09-13

    申请人： Jørn Borch Søe ,  Tina Lillan Jørgensen

    发明人： Jørn Borch Søe ,  Tina Lillan Jørgensen

    IPC分类号： A61K31/56 ,  C07J1/00 ,  C12P7/62 ,  A23L1/30 ,  C12P9/00

    CPC分类号： A61K31/575 ,  A23D7/01 ,  A23L33/11 ,  A61K8/63 ,  A61Q19/00 ,  C12P7/6436 ,  C12P7/6481 ,  C12P33/00

    摘要： The present invention relates to a method of producing a phytosterol ester and/or a phytostanol ester comprising: a) admixing a phospholipid composition comprising at least between about 10% to about 70% plant phospholipid and at least about 5% water; a lipid acyltransferase; and a phytosterol and/or a phytostanol; and b) separating or isolating or purifying at least one phytosterol ester and/or phytostanol ester from said admixture. The present invention also relates to compositions comprising the phytosterol ester and/or phytostanol ester produced by this method, including foodstuffs and personal care product (cosmetic) compositions.

    摘要翻译： 本发明涉及一种生产植物甾醇酯和/或植物甾烷醇酯的方法，包括：a）将包含至少约10％至约70％的植物磷脂和至少约5％的水的磷脂组合物混合; 脂质酰基转移酶; 植物甾醇和/或植物甾醇; 和b）从所述混合物中分离或分离或纯化至少一种植物甾醇酯和/或植物甾醇酯。 本发明还涉及包含通过该方法生产的植物甾醇酯和/或植物甾烷醇酯的组合物，包括食品和个人护理产品（化妆品）组合物。

    PROTEIN
    4.
    发明申请
    PROTEIN 有权
    蛋白

    公开(公告)号：US20100086640A1

    公开(公告)日：2010-04-08

    申请号：US12552572

    申请日：2009-09-02

    申请人： Janne Brunstedt ,  Jørn Dalgaard Mikkelsen ,  Henrik Pedersen ,  Jørn Borch Søe

    发明人： Janne Brunstedt ,  Jørn Dalgaard Mikkelsen ,  Henrik Pedersen ,  Jørn Borch Søe

    IPC分类号： A21D13/06 ,  A21D8/04

    CPC分类号： A21D8/042 ,  A23C19/0328 ,  A23D7/02 ,  A23K20/189 ,  A23K50/75 ,  A23L7/107 ,  A23L7/109 ,  A23L7/111 ,  A23L27/60 ,  A23L29/06 ,  C11B3/003 ,  C12N9/18 ,  C12P7/62 ,  C12P19/44 ,  C12Y301/01

    摘要： A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipid:triglyceride activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolyzing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.

    摘要翻译： 与甘油三酯相比，极性脂质活性比例更高的真菌野生型脂肪分解酶，其中所述酶优选具有至少为4的磷脂：甘油三酯活性比。优选地，根据本发明的脂肪分解酶具有糖脂： 甘油三酯水解活性比至少为1.5。 在一个实施方案中，根据本发明的真菌脂肪分解酶包含如SEQ ID NO：1或SEQ ID No.2或SEQ ID No.4或SEQ ID No.6所示的氨基酸序列或氨基酸序列， 与其具有至少90％的同一性。 本发明还包括编码真菌脂肪分解酶的核酸，该核酸选自：（a）包含SEQ ID No.3，SEQ ID No.5或SEQ ID No.5所示核苷酸的核酸 第7号 （b）通过遗传密码的退化与SEQ ID No.3，SEQ ID No.5或SEQ ID No.7的核苷酸序列有关的核酸; 和（c）核酸，其包含与SEQ ID No.3，SEQ ID No.5或SEQ ID No.7所示的核苷酸序列具有至少90％同一性的核苷酸序列。

    Fungal lipolytic enzymes, nucleic acids encoding, and uses thereof
    6.
    发明授权
    Fungal lipolytic enzymes, nucleic acids encoding, and uses thereof 有权
    真菌脂肪分解酶，编码的核酸及其用途

    公开(公告)号：US07622290B2

    公开(公告)日：2009-11-24

    申请号：US11519734

    申请日：2006-09-12

    申请人： Janne Brunstedt ,  Jørn Dalgaard Mikkelsen ,  Henrik Pedersen ,  Jørn Borch Søe

    发明人： Janne Brunstedt ,  Jørn Dalgaard Mikkelsen ,  Henrik Pedersen ,  Jørn Borch Søe

    IPC分类号： C12P1/00 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： A21D8/042 ,  A23C19/0328 ,  A23D7/02 ,  A23K20/189 ,  A23K50/75 ,  A23L7/107 ,  A23L7/109 ,  A23L7/111 ,  A23L27/60 ,  A23L29/06 ,  C11B3/003 ,  C12N9/18 ,  C12P7/62 ,  C12P19/44 ,  C12Y301/01

    摘要： A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipid:triglyceride activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolyzing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.

    摘要翻译： 与甘油三酯相比，极性脂质活性比例更高的真菌野生型脂肪分解酶，其中所述酶优选具有至少为4的磷脂：甘油三酯活性比。优选地，根据本发明的脂肪分解酶具有糖脂： 甘油三酯水解活性比至少为1.5。 在一个实施方案中，根据本发明的真菌脂肪分解酶包含如SEQ ID NO：1或SEQ ID No.2或SEQ ID No.4或SEQ ID No.6所示的氨基酸序列或氨基酸序列， 与其具有至少90％的同一性。 本发明还包括编码真菌脂肪分解酶的核酸，该核酸选自：（a）包含SEQ ID No.3，SEQ ID No.5或SEQ ID No.5所示核苷酸的核酸 第7号 （b）通过遗传密码的退化与SEQ ID No.3，SEQ ID No.5或SEQ ID No.7的核苷酸序列有关的核酸; 和（c）核酸，其包含与SEQ ID No.3，SEQ ID No.5或SEQ ID No.7所示的核苷酸序列具有至少90％同一性的核苷酸序列。

    Method
    9.
    发明授权
    Method 有权
    方法

    公开(公告)号：US07718408B2

    公开(公告)日：2010-05-18

    申请号：US10898775

    申请日：2004-07-26

    申请人： Paul Wassell ,  Jørn Borch Søe ,  Jørn Dalgaard Mikkelsen ,  Anna Cecilie Jentoft Kristensen

    发明人： Paul Wassell ,  Jørn Borch Søe ,  Jørn Dalgaard Mikkelsen ,  Anna Cecilie Jentoft Kristensen

    IPC分类号： C12P7/64 ,  C12Q1/48 ,  C12N9/10 ,  C12P21/06 ,  C07H21/04

    CPC分类号： C11C1/08 ,  C11C3/06 ,  C12N9/1029 ,  C12P7/6436 ,  C12P7/6445 ,  C12Y203/01

    摘要： The present application provides a method of reducing and/or removing diglyceride from an edible oil, comprising admixing an edible oil with an acyl acceptor substrate and a diglyceride: glycerol acyltransferase, wherein the diglyceride:glycerol acyltransferase is characterized as an enzyme which in an edible oil is capable of transferring an acyl group from a diglyceride to glycerol. The diglyceride:glycerol acyltransferase can comprise the amino acid sequence motif GDSX. The present invention also relates to the use of a diglyceride:glycerol acyltransferase in the manufacture of an edible oil, for reducing and/or removing diglyceride from said edible oil and to the use of said enzyme in the manufacture of a foodstuff comprising an edible oil for improving the crystallization properties of said foodstuff.

    摘要翻译： 本申请提供了从食用油中还原和/或除去甘油二酯的方法，包括将食用油与酰基受体底物和甘油二酯：甘油酰基转移酶混合，其中所述甘油二酯：甘油酰基转移酶被表征为可食用的酶 油能够将酰基从甘油二酯转移到甘油。 甘油二酯：甘油酰基转移酶可以包含氨基酸序列基序GDSX。 本发明还涉及甘油二酯：甘油酰基转移酶在制造食用油中的用途，用于从所述食用油中还原和/或除去甘油二酯，以及所述酶在制造包含食用油的食品中的用途 用于改善所述食品的结晶性能。