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    Lipid acyltransferases
    1.
    发明授权
    Lipid acyltransferases 有权
    脂质酰基转移酶

    公开(公告)号：US08030044B2

    公开(公告)日：2011-10-04

    申请号：US12040721

    申请日：2008-02-29

    申请人： Jørn Borch Søe ,  Jørn Dalgaard Mikkelson ,  Arno de Kreij

    发明人： Jørn Borch Søe ,  Jørn Dalgaard Mikkelson ,  Arno de Kreij

    IPC分类号： C12N9/10 ,  C07H21/04

    CPC分类号： C12N9/1029 ,  A21D8/042 ,  A23L15/25 ,  A23L33/10 ,  A23L33/195 ,  C11B3/003 ,  C12P7/62 ,  C12P7/6436 ,  C12P7/6454 ,  C12P7/6481

    摘要： The present invention relates to a method of producing a variant lipid acyltransferase enzyme by selecting a parent enzyme which is a lipid acyltransferase enzyme having the amino acid sequence motif GDSX, modifying one or more amino acids to produce a variant lipid acyltransferase, testing the variant lipid acyltransferase for activity on a galactolipid substrate, a phospholipid substrate and/or a triglyceride substrate, selecting a variant enzyme with an enhanced activity towards galactolipids compared with the parent enzyme, and/or preparing a quantity of the variant enzyme. In some embodiments, the variant lipid acyltransferase enzyme may include the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, X, Q, T N, M or S, and wherein the variant enzyme has one or more amino acid modifications compared with a parent sequence.

    摘要翻译： 本发明涉及通过选择具有氨基酸序列基序GDSX的脂质酰基转移酶的母体酶，修饰一个或多个氨基酸以产生变体脂质酰基转移酶来生产变体脂质酰基转移酶的方法，测试变体脂质 用于在半乳糖脂底物，磷脂底物和/或甘油三酯底物上的活性的酰基转移酶，与母体酶相比，选择具有增强的对乳糖脂的活性的变体酶和/或制备一定量的变体酶。 在一些实施方案中，变体脂质酰基转移酶可以包括氨基酸序列基序GDSX，其中X是以下氨基酸残基L，A，V，I，F，Y，X，Q，TN，M中的一个或多个或 S，并且其中所述变体酶与亲本序列相比具有一个或多个氨基酸修饰。

    Variant lipid acyltransferases and methods of making
    2.
    发明授权
    Variant lipid acyltransferases and methods of making 有权
    变体脂质酰基转移酶及其制备方法

    公开(公告)号：US07906307B2

    公开(公告)日：2011-03-15

    申请号：US11852274

    申请日：2007-09-07

    申请人： Jørn Borch Søe ,  Jørn Dalgaard Mikkelson ,  Arno de Kreij

    发明人： Jørn Borch Søe ,  Jørn Dalgaard Mikkelson ,  Arno de Kreij

    IPC分类号： G01N33/53 ,  C12Q1/34 ,  C12N9/00 ,  C12N9/16 ,  C12N9/10 ,  C07K14/00

    CPC分类号： C12P7/6463 ,  C12P7/6454

    摘要： The present invention relates to a method for the production of a variant lipid acyltransferase comprising the steps of: (i) selecting a parent enzyme which is a lipid acyltranserase enzyme; (ii) modifying one or more amino acids to produce a variant lipid acyltransferase; (iii) testing the activity of the variant lipid acyltransferase on a galactolipid and/or phospholipid and/or triglyceride substrate; (iv) selecting a variant enzyme with enhanced activity towards galactolipids compared with the parent enzyme; (v) providing a Bacillus licheniformis cell; (vi) transforming the Bacillus licheniformis cell with a heterologous nucleotide sequence encoding said variant lipid acyltransferase; and (iii) expressing said variant lipid acyltransferase in the cell under the control of a promoter sequence. The variant lipid acyltransferase can undergo post-translations modification, truncation and/or clipping, i.e., to remove a signal peptide. In addition, the present invention further relates to the use of Bacillus licheniformis to express a lipid acyltransferase, a Bacillus licheniformis host cell comprising a heterologous lipid acyltransferase and a vector comprising a nucleotide sequence encoding a lipid acyltransferase operably linked to a promoter sequence homologous to B. licheniformis.

    摘要翻译： 本发明涉及生产变体脂质酰基转移酶的方法，包括以下步骤：（i）选择脂质酰基转移酶的亲本酶; （ii）修饰一个或多个氨基酸以产生变体脂质酰基转移酶; （iii）测试变体脂质酰基转移酶对半乳糖脂和/或磷脂和/或甘油三酯底物的活性; （iv）与亲本酶相比，选择具有增强的对半乳糖脂的活性的变体酶; （v）提供地衣芽孢杆菌细胞; （vi）用编码所述变体脂质酰基转移酶的异源核苷酸序列转化地衣芽孢杆菌细胞; 和（iii）在启动子序列控制下的细胞中表达所述变体脂质酰基转移酶。 变体脂质酰基转移酶可经历翻译后修饰，截短和/或剪切，即去除信号肽。 此外，本发明还涉及地衣芽孢杆菌表达脂质酰基转移酶，包含异源脂质酰基转移酶的地衣芽孢杆菌宿主细胞的使用和包含编码脂质酰基转移酶的核苷酸序列的载体，所述核苷酸序列可操作地连接到与B同源的启动子序列 地衣芽孢杆菌

    Method for producing ultra-heat treatment milk
    10.
    发明授权
    Method for producing ultra-heat treatment milk 有权
    生产超热处理牛奶的方法

    公开(公告)号：US08652809B2

    公开(公告)日：2014-02-18

    申请号：US12706210

    申请日：2010-02-16

    申请人： Niels Erik Larsen ,  Jørn Borch Søe

    发明人： Niels Erik Larsen ,  Jørn Borch Søe

    IPC分类号： C12P19/58

    CPC分类号： A21D8/042 ,  A23C19/0328 ,  A23C19/0684 ,  A23L15/25 ,  A23L29/06 ,  C12N9/1029 ,  C12N9/18 ,  C12N11/00 ,  C12Y301/01

    摘要： The invention relates to using a lipid acyltransferase polypeptide (SEQ ID NO: 68) in the manufacture of ultra-heat treatment milk (UHT) for improving the stability, the perceptible sensory difference, the smell, and the taste; and for reducing the cholesterol content, and for eliminating or reducing creaming of the UHT milk. The invention also relates to a method of producing UHT milk, wherein the method comprises admixing a lipid acyltransferase with milk and heating the mixture to make it a UHT milk.

    摘要翻译： 本发明涉及使用脂质酰基转移酶多肽（SEQ ID NO：68）制造超热处理牛奶（UHT），用于改善稳定性，可察觉的感觉差异，气味和味道; 并且用于降低胆固醇含量，以及用于消除或减少UHT牛奶的奶油。 本发明还涉及一种生产UHT乳的方法，其中所述方法包括将脂质酰基转移酶与乳混合并加热混合物以使其成为UHT牛奶。