公开(公告)号：US11965891B2

公开(公告)日：2024-04-23

申请号：US15393445

申请日：2016-12-29

Applicant: Bio-Rad Laboratories, Inc. ,  Bio-Rad Europe Gmbh

Inventor： Jeremy Agresti ,  Ronald Lebofsky

IPC: G01N33/68 ,  B01J19/00 ,  C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6869 ,  C40B20/04 ,  C40B70/00

CPC classification number: G01N33/6878 ,  B01J19/0046 ,  C12Q1/6816 ,  C12Q1/6869 ,  C40B20/04 ,  C40B70/00 ,  C12Q1/6816 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q1/6869 ,  C12Q2565/514

Abstract: Methods and compositions are described for single cell resolution, quantitative proteomic analysis using high throughput sequencing.

公开(公告)号：US11905551B2

公开(公告)日：2024-02-20

申请号：US17508674

申请日：2021-10-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jeremy Agresti ,  George Karlin-Neumann

IPC: C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6832 ,  C12Q1/682

CPC classification number: C12Q1/6806 ,  C12Q1/682 ,  C12Q1/686 ,  C12Q1/6811 ,  C12Q1/6832 ,  C12Q1/6853 ,  C12Q1/6853 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2531/119 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/537

Abstract: Improved multiple displacement amplification (MDA) reagents and methods are provided.

公开(公告)号：US11834710B2

公开(公告)日：2023-12-05

申请号：US17136824

申请日：2020-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jennifer Chew

IPC: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6862 ,  C12Q1/6876 ,  C12Q1/6848 ,  C12Q1/686 ,  C12N15/10 ,  C12N9/12 ,  C12Q1/6806

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12N15/1003 ,  C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/6855 ,  C12Q1/6862 ,  C12Q1/6876 ,  C12Q2563/185 ,  C12Y207/07007 ,  C12Q1/6806 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: The methods and reagents are provided for barcoding and analysis of DNA samples using partition (e.g., droplet) technology while avoiding performing amplification in droplets.

公开(公告)号：US11759761B2

公开(公告)日：2023-09-19

申请号：US17381096

申请日：2021-07-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Nicholas Heredia

IPC: B01J19/00 ,  C12Q1/6806

CPC classification number: B01J19/0046 ,  C12Q1/6806 ,  B01J2219/00547 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12Q1/6806 ,  C12Q2525/131 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q2565/519 ,  C12Q1/6806 ,  C12Q2523/319 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q2565/519 ,  C12Q1/6806 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q2565/519

Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.

公开(公告)号：US20230100295A1

公开(公告)日：2023-03-30

申请号：US18075009

申请日：2022-12-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Ronald Lebofsky

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/686

Abstract: The invention(s) cover systems and methods for target detection in a multiplexed and rapid manner. Embodiments of the system can include: a base substrate; and an array of sample processing regions defined at a broad surface of the base substrate, wherein each of the array of sample processing regions includes: a set of microwell subarrays arranged in a gradient by volumetric capacity between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions. The system can support methods, with example implementation by an automated platform, for returning preliminary results from a subset of microwells of the samples processing regions, as well as results pertaining to specific and non-specific amplification, for multiple targets of a sample.

公开(公告)号：US11603556B2

公开(公告)日：2023-03-14

申请号：US17087952

申请日：2020-11-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky

IPC: C12Q1/68 ,  C12Q1/6834 ,  C12Q1/6855

Abstract: Methods and compositions for attaching cell-specific barcodes without formation of partitions is provided.

公开(公告)号：US11479816B2

公开(公告)日：2022-10-25

申请号：US16545611

申请日：2019-08-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Andrew Kohlway ,  Jennifer Chew ,  Zachary Burkett ,  Man Cheng

IPC: C12Q1/6869 ,  G16B40/10 ,  G16B30/10

Abstract: Comparison of common sequencing reads from sequencing based on partition-based barcoding can be used to improve sequencing results. Increased loading of barcodes per partition can also improve sequencing results.

公开(公告)号：US11021738B2

公开(公告)日：2021-06-01

申请号：US15847408

申请日：2017-12-19

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jeremy Agresti

IPC: C12P19/34 ,  C12Q1/6809 ,  G16B20/00 ,  C12Q1/6869

Abstract: A method of maintaining contiguity in chromosomal DNA following treatment with a tagmentase. Conditions are selected such that the tagmentase does not release from the DNA, and thus forms a bridge linking DNA segments that have the same relationship (haplotype) as occurred in the genomic DNA. Thus the tagmentase step can occur in bulk (before partitions are formed). The resulting tagmentase-bridged DNA segments can be added to partitions maintaining the bridged segments until they are introduced into different partitions. Once in partitions, the contiguous DNA segments can be barcoded with a partition-specific barcode, thereby allowing for later identification of contiguous DNA after sequencing in bulk (after partitions contents are merged).

公开(公告)号：US10907205B2

公开(公告)日：2021-02-02

申请号：US16178313

申请日：2018-11-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jennifer Chew

IPC: C12P19/34 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6862 ,  C12Q1/6876 ,  C12Q1/6848 ,  C12Q1/686 ,  C12N15/10 ,  C12N9/12 ,  C12Q1/6806 ,  C12Q1/68

Abstract: The methods and reagents are provided for barcoding and analysis of DNA samples using partition (e.g., droplet) technology while avoiding performing amplification in droplets.