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公开(公告)号：US5686600A
公开(公告)日：1997-11-11
申请号：US442542
申请日：1995-05-16
IPC分类号： A01H5/00 , A01N63/02 , A61K47/48 , C07K14/21 , C07K14/325 , C07K16/18 , C07K19/00 , C12N1/21 , C12N5/10 , C12N15/02 , C12N15/09 , C12N15/13 , C12N15/82 , C12P21/08 , C07H21/04 , C07K16/00 , C07K16/46
CPC分类号： A01N63/02 , A61K47/48438 , A61K47/48538 , A61K47/48546 , A61K47/48561 , C07K14/21 , C07K14/325 , C07K16/18 , C07K19/00 , C12N15/8286 , C07K2317/622 , C07K2319/00
摘要： Antibodies, monoclonal antibodies or fragments thereof which bind to brush border membrane vesicles of insect gut and the gene or genes which encode these proteins are provided. The monoclonal antibodies bind the gut of a target insect but do not bind to mammalian brush border membranes or to plant microsomes. The antibodies and the genes encoding them find use in constructing hybrid toxins for control of insect pests.
摘要翻译： 提供了结合昆虫肠的刷状膜囊泡的抗体，单克隆抗体或其片段以及编码这些蛋白质的基因或基因。 单克隆抗体结合目标昆虫的肠，但不结合哺乳动物刷状膜或植物微粒体。 抗体和编码它们的基因用于构建用于控制害虫的杂交毒素。
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32.发明授权
公开(公告)号：US5625136A
公开(公告)日：1997-04-29
申请号：US951715
申请日：1992-09-25
申请人： Michael G. Koziel , Nalini M. Desai , Kelly S. Lewis , Vance C. Kramer , Gregory W. Warren , Stephen V. Evola , Lyle D. Crossland , Martha S. Wright , Ellis J. Merlin , Karen L. Launis , Steven J. Rothstein , Cindy G. Bowman , John L. Dawson , Erik M. Dunder , Gary M. Pace , Janet L. Suttie
发明人： Michael G. Koziel , Nalini M. Desai , Kelly S. Lewis , Vance C. Kramer , Gregory W. Warren , Stephen V. Evola , Lyle D. Crossland , Martha S. Wright , Ellis J. Merlin , Karen L. Launis , Steven J. Rothstein , Cindy G. Bowman , John L. Dawson , Erik M. Dunder , Gary M. Pace , Janet L. Suttie
IPC分类号： A01H5/00 , A01H5/10 , C07H21/04 , C07K14/325 , C12N5/04 , C12N9/12 , C12N9/88 , C12N15/09 , C12N15/11 , C12N15/32 , C12N15/54 , C12N15/60 , C12N15/82 , C12N15/87
CPC分类号： C07K14/325 , C12N15/8286 , C12N9/12 , C12N9/88
摘要： DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.
摘要翻译： 公开了优化用于在植物中表达的DNA序列。 DNA序列优选编码杀虫多肽，特别是来自苏云金芽孢杆菌的杀虫蛋白。 还提供植物启动子，特定组织特异性和组织优选启动子。 另外公开了包含所述DNA序列的转化载体。 转化载体在转化为玉米时表现出高水平的杀虫活性。
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公开(公告)号：US08829279B2
公开(公告)日：2014-09-09
申请号：US13168454
申请日：2011-06-24
申请人： Nadine Carozzi , Michael G. Koziel , Nicholas Duck , Nalini M. Desai , Rong Guo , Daniel John Tomso , Rebekah Deter , Tracy Hargiss
发明人： Nadine Carozzi , Michael G. Koziel , Nicholas Duck , Nalini M. Desai , Rong Guo , Daniel John Tomso , Rebekah Deter , Tracy Hargiss
IPC分类号： A01H5/00 , C12N15/82 , C07K14/325 , A01N37/18
CPC分类号： C12N15/8286 , A01N37/44 , C07K14/325 , C12P21/02 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号NRRL B-30961，B-30955，B-30956，B-30957，B-30958，B -30942，B-30939，B-30941，B-50047，B-30959，B-30960，B-30943或B-50048，以及其变体和片段。
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公开(公告)号：US08563808B2
公开(公告)日：2013-10-22
申请号：US11765491
申请日：2007-06-20
CPC分类号： C07H21/04 , C07K14/325
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof.
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35.发明授权AXMI-115, AXMI-113, AXMI-005, AXMI-163 and AXMI-184: insecticidal proteins and methods for their use 有权AXMI-115，AXMI-113，AXMI-005，AXMI-163和AXMI-184：其杀虫蛋白及其使用方法公开(公告)号：US08334431B2
公开(公告)日：2012-12-18
申请号：US12497221
申请日：2009-07-02
申请人： Kimberly S. Sampson , Daniel J. Tomso , Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Shruti Agarwal , Brian McNulty , Chris Campbell , Volker Heinrichs
发明人： Kimberly S. Sampson , Daniel J. Tomso , Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Shruti Agarwal , Brian McNulty , Chris Campbell , Volker Heinrichs
CPC分类号： C12N15/8286 , A01N37/18 , C07K14/32 , C07K14/325 , C07K16/1278 , Y02A40/162
摘要： Compositions and methods for conferring insecticidal activity to host cells are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in host cells. Compositions also comprise transformed host cells. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 6, 13, or 14, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 11, or 12, as well as variants and fragments thereof.
摘要翻译： 提供了赋予宿主细胞杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在宿主细胞中转化和表达。 组合物还包含转化的宿主细胞。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：4,5,6,13或14所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 2,3,11或12，以及其变体和片段。
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公开(公告)号：US08133858B2
公开(公告)日：2012-03-13
申请号：US12026427
申请日：2008-02-05
IPC分类号： C07K14/325 , A01N37/18
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin and delta-endotoxin-associated polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin and delta-endotoxin-associated nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3, 5, and 7, and the nucleotide sequences set forth in SEQ ID NO:1, 2, 4, and 6, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素和δ-内毒素相关多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素和δ-内毒素相关的核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3,5和7所示氨基酸序列的核苷酸序列，以及SEQ ID NO： 和6，以及其变体和片段。
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公开(公告)号：US20110263488A1
公开(公告)日：2011-10-27
申请号：US13168454
申请日：2011-06-24
申请人： Nadine Carozzi , Michael G. Koziel , Nicholas B. Duck , Nalini M. Desai , Rong Guo , Daniel John Tomso , Rebekah Deter , Tracy Hargiss
发明人： Nadine Carozzi , Michael G. Koziel , Nicholas B. Duck , Nalini M. Desai , Rong Guo , Daniel John Tomso , Rebekah Deter , Tracy Hargiss
IPC分类号： A01N63/02 , C12N15/63 , C12N1/21 , A01H5/10 , A01H5/00 , C07K14/325 , A01P5/00 , A01P7/04 , C07H21/04 , C12N5/10
CPC分类号： C12N15/8286 , A01N37/44 , C07K14/325 , C12P21/02 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号NRRL B-30961，B-30955，B-30956，B-30957，B-30958，B -30942，B-30939，B-30941，B-50047，B-30959，B-30960，B-30943或B-50048，以及其变体和片段。
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38.发明授权公开(公告)号：US07811598B2
公开(公告)日：2010-10-12
申请号：US12166253
申请日：2008-07-01
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 15, 17, or 19, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 14, 16, or 18, as well as variants and fragments thereof.
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公开(公告)号：US07807881B2
公开(公告)日：2010-10-05
申请号：US11588811
申请日：2006-10-27
CPC分类号： C12N15/8274 , C12N9/88 , C12N15/8275
摘要： Compositions and methods for conferring herbicide resistance to plant cells and bacterial cells are provided. The methods comprise transforming the cells with nucleotide sequences encoding herbicide resistance genes. In particular, herbicide resistance is conferred by expression of proteins with homology to decarboxylase enzymes. Compositions comprise transformed plants, plant tissues, and seeds, as well as transformed bacterial cells.
摘要翻译： 提供了赋予对植物细胞和细菌细胞的除草剂抗性的组合物和方法。 所述方法包括用编码除草剂抗性基因的核苷酸序列转化细胞。 特别地，通过与脱羧酶具有同源性的蛋白质的表达赋予除草剂抗性。 组合物包括转化的植物，植物组织和种子，以及转化的细菌细胞。
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公开(公告)号：US20100138961A1
公开(公告)日：2010-06-03
申请号：US12701567
申请日：2010-02-07
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO:1, as well as variants thereof.
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