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公开(公告)号：US5834201A
公开(公告)日：1998-11-10
申请号：US893523
申请日：1997-07-11
申请人： James L. Hartley
发明人： James L. Hartley
摘要： The invention relates to a nucleic acid marker ladder which is a restriction endonuclease digest, wherein a nucleic acid restriction endonuclease digest is a collection of nucleic acid fragments resulting from complete digestion of one or more nucleic acids by one or more restriction endonucleases; the restriction endonuclease digest contains at least 3 fragments; and the size of the fragments in base pairs is a multiple of an integer, wherein the integer is 10 or more.
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公开(公告)号：US5434066A
公开(公告)日：1995-07-18
申请号：US214023
申请日：1994-03-15
申请人： Robert L. Bebee , James L. Hartley
发明人： Robert L. Bebee , James L. Hartley
摘要： Methods and recombinant vectors suitable for accomplishing the in vivo alteration of a nucleic acid molecule are disclosed. The invention in particular discloses the use of recombinases such as Cre to accomplish in vivo recombination.
摘要翻译： 公开了适于实现核酸分子的体内改变的方法和重组载体。 本发明特别公开了使用重组酶如Cre来完成体内重组。
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公开(公告)号：US06924098B2
公开(公告)日：2005-08-02
申请号：US09472067
申请日：1999-12-23
申请人： A-Li W. Hu , James L. Hartley , Heather J. Jordan
发明人： A-Li W. Hu , James L. Hartley , Heather J. Jordan
CPC分类号： C07H21/00 , C12N15/10 , C12Q1/68 , C12Q1/6827 , C12Q1/6876
摘要： The present invention provides nucleic acid molecules which may be used as standards for estimating the size (in base pairs) and mass of linear, double-stranded or single-stranded nucleic acid molecules separated by size. The nucleic acid molecules of the invention may be DNA molecules, RNA molecules or DNA/RNA hybrid molecules, and may be double-stranded or single-stranded. The invention also provides methods for producing nucleic acid sizing ladders from these nucleic acid molecules, ladders produced by such methods, and methods for estimating the size and mass of nucleic acid molecules by comparison to these nucleic acid sizing ladders.
摘要翻译： 本发明提供核酸分子，其可以用作估计由大小分开的线性，双链或单链核酸分子的大小（碱基对）和质量的标准。 本发明的核酸分子可以是DNA分子，RNA分子或DNA / RNA杂交分子，并且可以是双链或单链的。 本发明还提供了用于从这些核酸分子生产核酸筛选梯级的方法，通过这些方法产生的梯子，以及通过与这些核酸分级梯相比较估计核酸分子的大小和质量的方法。
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34.发明授权公开(公告)号：US06287823B1
公开(公告)日：2001-09-11
申请号：US09344491
申请日：1999-06-25
申请人： James L. Hartley
发明人： James L. Hartley
IPC分类号： C12P1934
CPC分类号： C12Q1/6848 , C12Q1/6853 , C12Q1/708 , Y10S435/81 , C12Q2525/117 , C12Q2521/531 , C12Q2531/113 , C12Q2525/186 , C12Q2531/137 , C12Q2521/301 , C12Q2537/137 , C12Q2525/101
摘要： This invention relates to a method of incorporating an exo-sample nucleotide into the amplified product strands resulting from a nucleic acid amplification process. Once the product strands have been obtained and analyzed (e.g., by hybridization, Southern blot, etc.), the exo-sample strands can be selectively destroyed by acting on the incorporated exo-sample nucleotide. Two embodiments are presented. In a first embodiment, the exo-sample nucleotide is incorporated by carrying out the amplification reaction in the presence of an excess of exo-sample nucleotide triphosphate. In a second embodiment, the exo-sample nucleotide is incorporated by carrying out the amplification reaction in the presence of an oligonucleotide which has, as part of its sequence, one or more exo-sample nucleotides.
摘要翻译： 本发明涉及将外切样品核苷酸掺入由核酸扩增过程产生的扩增产物链中的方法。 一旦获得并分析了产物链（例如通过杂交，Southern印迹等），可以通过作用于外加的外切核苷酸来选择性地破坏外部样品链。提供了两个实施方案。 在第一个实施方案中，通过在过量样品中的三磷酸过多的存在下进行扩增反应来引入外切核苷酸。在第二个实施方案中，通过进行扩增反应来引入外切核苷酸 作为其序列的一部分具有一个或多个外切核苷酸的寡核苷酸的存在。
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35.发明授权
公开(公告)号：US5683896A
公开(公告)日：1997-11-04
申请号：US221465
申请日：1994-04-01
申请人： James L. Hartley , Mark Berninger
发明人： James L. Hartley , Mark Berninger
CPC分类号： C12Q1/6848 , C12Q1/6853 , C12Q1/708 , Y10S435/81
摘要： This invention relates to a method of incorporating an exo-sample nucleotide into the amplified product strands resulting from a nucleic acid amplification process. Once the product strands have been obtained and analyzed (e.g., by hybridization, Southern blot, etc.), the exo-sample strands can be selectively destroyed by acting on the incorporated exo-sample nucleotide. Two embodiments are presented. In a first embodiment, the exo-sample nucleotide is incorporated by carrying out the amplification reaction in the presence of an excess of exo-sample nucleotide triphosphate. In a second embodiment, the exo-sample nucleotide is incorporated by carrying out the amplification reaction in the presence of an oligonucleotide which has, as part of its sequence, one or more exo-sample nucleotides.
摘要翻译： 本发明涉及将外切样品核苷酸掺入由核酸扩增过程产生的扩增产物链中的方法。 一旦获得并分析了产物链（例如通过杂交，Southern印迹等），可以通过作用于外加的外切核苷酸来选择性地破坏外部样品链。 呈现两个实施例。 在第一个实施方案中，通过在过量样品核苷酸三磷酸的存在下进行扩增反应来引入外切核苷酸。 在第二个实施方案中，通过在寡核苷酸的存在下进行扩增反应来引入外切核苷酸，所述寡核苷酸作为其序列的一部分，具有一个或多个外切核苷酸。
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公开(公告)号：US5449758A
公开(公告)日：1995-09-12
申请号：US160670
申请日：1993-12-02
申请人： James L. Hartley
发明人： James L. Hartley
CPC分类号： C07K1/26 , C07K14/005 , C07K14/80 , C12N15/62 , C07K2319/00 , C07K2319/21 , C12N2795/10122 , Y10S435/81
摘要： The invention relates to a multidomain protein comprising sites for cleavage of the protein into at least 3 polypeptide domains; nucleic acid encoding the multidomain protein; a protein ladder comprising a collection of protein fragments obtained by the partial cleavage of one or more multidomain proteins by one or more cleaving agents; and methods of using and preparing the protein ladder.
摘要翻译： 本发明涉及包含用于将蛋白切割成至少3个多肽结构域的位点的多结构域蛋白; 编码多结构域蛋白的核酸; 包括通过一种或多种裂解剂部分切割一个或多个多结构域蛋白而获得的蛋白质片段的集合的蛋白梯子; 以及使用和制备蛋白质梯的方法。
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37.发明授权
公开(公告)号：US5035996A
公开(公告)日：1991-07-30
申请号：US360120
申请日：1989-06-01
申请人： James L. Hartley
发明人： James L. Hartley
CPC分类号： C12Q1/708 , C12Q1/6848
摘要： In the process according to this invention, an amplification procedure is performed on a first sample in which one or more of the four normal ribonucleoside triphosphates (rNTPs) or deoxyribonucleoside triphosphates (dNTPs) is replaced with an exo-sample nucleotide. After amplification, any contaminating amplified product that may be remaining is subjected to a physical, chemical, enzymatic, or biological treatment which renders nucleic acid containing the exo-sample nucleotide substantially unamplifiable. The treatment may be done as a separate step or it may be done in the presence of a second sample containing nucleic acid sequences to be amplified. The amplified nucleic acid sequences derived from the first sample which contaminate the second sample are not further substantially amplified during amplification of nucleic acid sequences of the second sample.
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公开(公告)号：US20090186387A1
公开(公告)日：2009-07-23
申请号：US12247861
申请日：2008-10-08
申请人： James L. Hartley , Michael Brasch
发明人： James L. Hartley , Michael Brasch
IPC分类号： C12P19/34
摘要： Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
摘要翻译： 通过在体外和体内使用核酸，载体和方法，使用工程化重组位点和重组蛋白来移动或交换DNA分子片段来提供重组克隆，以提供具有所需特征的嵌合DNA分子和 /或DNA片段。
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公开(公告)号：US07132520B2
公开(公告)日：2006-11-07
申请号：US10410176
申请日：2003-04-10
申请人： James L. Hartley
发明人： James L. Hartley
摘要： The invention relates to a nucleic acid marker ladder which is a restriction endonuclease digest, wherein a nucleic acid restriction endonuclease digest is a collection of nucleic acid fragments resulting from complete digestion of one or more nucleic acids by one or more restriction endonucleases; the restriction endonuclease digest contains at least 3 fragments; and the size of the fragments in base pairs is a multiple of an integer, wherein the integer is 10 or more.
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公开(公告)号：US06680378B1
公开(公告)日：2004-01-20
申请号：US09666890
申请日：2000-09-20
申请人： James L. Hartley
发明人： James L. Hartley
IPC分类号： C07H1900
摘要： The invention relates to a nucleic acid marker ladder which is a restriction endonuclease digest, wherein a nucleic acid restriction endonuclease digest is a collection of nucleic acid fragments resulting from complete digestion of one or more nucleic acids by one or more restriction endonucleases; the restriction endonuclease digest contains at least 3 fragments; and the size of the fragments in base pairs is a multiple of an integer, wherein the integer is 10 or more.
摘要翻译： 本发明涉及作为限制性内切核酸酶消化物的核酸标记梯，其中核酸限制性内切核酸酶消化物是由一种或多种限制性内切核酸酶完全消化一种或多种核酸而产生的核酸片段的集合; 限制性内切酶消化物包含至少3个片段; 并且碱基对中的片段的大小是整数的倍数，其中整数为10以上。
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