公开(公告)号：US07514260B2

公开(公告)日：2009-04-07

申请号：US11134564

申请日：2005-05-20

申请人： Ren-He Xu ,  James A. Thomson

发明人： Ren-He Xu ,  James A. Thomson

IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/08

CPC分类号： C12N5/0606 ,  C12N2501/115 ,  C12N2501/155

摘要： Previous methods for culturing human embryonic stem cells have required either fibroblast feeder cells or a medium which has been exposed to fibroblast feeder cells in order to maintain the stem cells in an undifferentiated state. It has now been found that if an antagonist of bone morphogenic protein is added to the medium in which the stem cells are cultured, together with fibroblast growth factor, the stem cells will remain undifferentiated indefinitely, even without feeder cells or conditioned medium.

摘要翻译： 用于培养人胚胎干细胞的以前的方法需要成纤维细胞饲养细胞或已经暴露于成纤维细胞饲养细胞的培养基，以便保持干细胞处于未分化状态。 现在已经发现，如果将骨形态发生蛋白的拮抗剂加入到其中培养干细胞的培养基中，与成纤维细胞生长因子一起，干细胞将无限期地保持未分化，即使没有饲养细胞或条件培养基。

公开(公告)号：US20090011503A1

公开(公告)日：2009-01-08

申请号：US12047135

申请日：2008-03-12

申请人： James A. Thomson

发明人： James A. Thomson

IPC分类号： C12N5/08 ,  C12N5/02

CPC分类号： C12N5/0605 ,  C12N5/0603 ,  C12N5/0606 ,  C12N2502/13 ,  C12N2506/02

摘要： A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.

摘要翻译： 公开了灵长类动物胚胎干细胞的纯化制剂。 该制剂的特征在于以下细胞表面标志物：SSEA-1（ - ）; SSEA-4（+）; TRA-1-60（+）; TRA-1-81（+）; 碱性磷酸酶（+）。 在一个特别有利的实施方案中，制剂的细胞是人胚胎干细胞，具有正常的核型，并且在连续培养11个月后，以未分化状态继续增殖。 胚胎干细胞系还保留了整个培养物中形成滋养细胞并分化成来自所有三个胚胎胚层（内胚层，中胚层和外胚层）的所有组织的能力。 还公开了分离灵长类动物胚胎干细胞系的方法。

公开(公告)号：US07449334B2

公开(公告)日：2008-11-11

申请号：US11221457

申请日：2005-09-08

申请人： James A. Thomson ,  Tenneille Ludwig

发明人： James A. Thomson ,  Tenneille Ludwig

IPC分类号： C12N5/08 ,  C12N5/02

CPC分类号： C12N5/0606 ,  C12N5/0696 ,  C12N2500/12 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/50 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/845

摘要： Previous methods for culturing human embryonic stem cells have required either fibroblast feeder cells or a medium which has been exposed to fibroblast feeder cells in order to maintain the stem cells in an undifferentiated state. It has now been found that if high levels of fibroblast growth factor, gamma amino butyric acid, pipecholic acid, lithium and transforming growth factor beta are added to the medium in which the stem cells are cultured, the stem cells will remain undifferentiated indefinitely through multiple passages, even without feeder cells or conditioned medium.

摘要翻译： 用于培养人胚胎干细胞的以前的方法需要成纤维细胞饲养细胞或已经暴露于成纤维细胞饲养细胞的培养基，以便保持干细胞处于未分化状态。 现在已经发现，如果将高水平的成纤维细胞生长因子，γ氨基丁酸，哌醋酸，锂和转化生长因子β加入到其中培养干细胞的培养基中，则干细胞将通过多次而无限期地保持未分化 通道，甚至没有饲养细胞或条件培养基。

公开(公告)号：US06200806B1

公开(公告)日：2001-03-13

申请号：US09106390

申请日：1998-06-26

申请人： James A. Thomson

发明人： James A. Thomson

IPC分类号： C12N508

CPC分类号： C12N5/0605 ,  C12N5/0603 ,  C12N5/0606 ,  C12N2502/13 ,  C12N2506/02

摘要： A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.

公开(公告)号：US08440461B2

公开(公告)日：2013-05-14

申请号：US12053440

申请日：2008-03-21

申请人： James A. Thomson ,  Junying Yu

发明人： James A. Thomson ,  Junying Yu

IPC分类号： C12N15/00

CPC分类号： C12N5/0607 ,  C12N5/0606 ,  C12N5/0696 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2502/99 ,  C12N2510/00 ,  C12N2799/027

摘要： The present invention relates to methods for reprogramming a somatic cell to pluripotency by administering into the somatic cell at least one or a plurality of potency-determining factors. The invention also relates to pluripotent cell populations obtained using a reprogramming method.

摘要翻译： 本发明涉及通过向体细胞施用至少一种或多种效力决定因素来将体细胞重编程为多能性的方法。 本发明还涉及使用重编程方法获得的多能细胞群体。

公开(公告)号：US20120328582A1

公开(公告)日：2012-12-27

申请号：US13595587

申请日：2012-08-27

申请人： James A. Thomson

发明人： James A. Thomson

IPC分类号： A61K35/12 ,  A61P37/00 ,  A61P3/10 ,  C12N5/0735 ,  A61P25/16

CPC分类号： C12N5/0605 ,  C12N5/0603 ,  C12N5/0606 ,  C12N2502/13 ,  C12N2506/02

摘要： A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.

摘要翻译： 公开了灵长类动物胚胎干细胞的纯化制剂。 该制剂的特征在于以下细胞表面标志物：SSEA-1（ - ）; SSEA-4（+）; TRA-1-60（+）; TRA-1-81（+）; 和碱性磷酸酶（+）。 在一个特别有利的实施方案中，制剂的细胞是人胚胎干细胞，具有正常的核型，并且在连续培养11个月后，以未分化状态继续增殖。 胚胎干细胞系还保留了整个培养物中形成滋养细胞并分化成来自所有三个胚胎胚层（内胚层，中胚层和外胚层）的所有组织的能力。 还公开了分离灵长类动物胚胎干细胞系的方法。

公开(公告)号：US20120178160A1

公开(公告)日：2012-07-12

申请号：US13398933

申请日：2012-02-17

申请人： James A. Thomson ,  Mark Levenstein ,  Ren-He Xu

发明人： James A. Thomson ,  Mark Levenstein ,  Ren-He Xu

IPC分类号： C12N5/0735

CPC分类号： C12N5/0606 ,  C12N2500/44 ,  C12N2501/115 ,  C12N2501/155

摘要： The invention relates to methods for culturing human embryonic stem cells by culturing the stem cells in an environment essentially free of mammalian fetal serum and in a stem cell culture medium including amino acids, vitamins, salts, minerals, transferrin, insulin, albumin, and a fibroblast growth factor that is supplied from a source other than just a feeder layer the medium. Also disclosed are compositions capable of supporting the culture and proliferation of human embryonic stem cells without the need for feeder cells or for exposure of the medium to feeder cells.

摘要翻译： 本发明涉及通过在基本上不含哺乳动物胎儿血清的环境中培养干细胞以及在含有氨基酸，维生素，盐，矿物质，转铁蛋白，胰岛素，白蛋白和干扰素的干细胞培养基中培养人胚胎干细胞的方法。 成纤维细胞生长因子从源而不仅仅是饲养层提供给培养基。 还公开了能够支持人胚胎干细胞的培养和增殖而不需要饲养细胞或将培养基暴露于饲养细胞的组合物。

公开(公告)号：US08012751B2

公开(公告)日：2011-09-06

申请号：US11395657

申请日：2006-03-31

申请人： James A. Thomson ,  Thomas P. Zwaka

发明人： James A. Thomson ,  Thomas P. Zwaka

IPC分类号： C12N5/00

CPC分类号： C12N5/0606 ,  C12N2501/48 ,  C12N2501/70

摘要： The invention relates to a method to induce primate embryonic stem cells to differentiate into a relatively homogenous population of mesendoderm cells by treatment with caspase-like inhibitors. Also described is a population of mesendoderm cells obtained therefrom. The embryonic stem cell derived mesendoderm cells have the general morphological and cell surface marker characteristics of mesendoderm cells.

摘要翻译： 本发明涉及通过用半胱天冬酶样抑制剂处理来诱导灵长类动物胚胎干细胞分化为相对均匀的中内胚层细胞群的方法。 还描述了从其获得的中胚层细胞群。 胚胎干细胞衍生的中胚层细胞具有中内根异质细胞的一般形态和细胞表面标志物特征。

公开(公告)号：US20110117135A1

公开(公告)日：2011-05-19

申请号：US12876830

申请日：2010-09-07

申请人： Igor I. Slukvin ,  James A. Thomson ,  Maksym A. Vodyanyk ,  Maryna E. Gumenyuk

发明人： Igor I. Slukvin ,  James A. Thomson ,  Maksym A. Vodyanyk ,  Maryna E. Gumenyuk

IPC分类号： A61K39/00 ,  C12N5/0784 ,  C12N5/071 ,  A61P37/04 ,  A61P35/00

CPC分类号： C12N5/0639 ,  C12N5/064 ,  C12N2506/02

摘要： This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers.

摘要翻译： 本发明涉及来自人胚胎干（ES）细胞的树突状细胞的培养。 首先通过与基质细胞共培养将人类ES细胞培养成造血细胞。 然后将分化成造血谱系的细胞与GM-CSF一起培养以产生骨髓前体细胞的培养物。 用细胞因子GM-CSF和IL-4培养骨髓前体细胞会导致产生功能性树突状细胞。 树突细胞具有独特的表型，如细胞表面标志物的组合所示。

公开(公告)号：US20100304481A1

公开(公告)日：2010-12-02

申请号：US12754574

申请日：2010-04-05

申请人： James A. Thomson ,  Tenneille Ludwig

发明人： James A. Thomson ,  Tenneille Ludwig

IPC分类号： C12N5/0735 ,  C12N5/071 ,  C12N5/02

CPC分类号： C12N5/0606 ,  C12N5/0696 ,  C12N2500/12 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/50 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/845

摘要： Previous methods for culturing primate pluripotent stem cells have required either fibroblast feeder cells or a medium which was exposed to fibroblast feeder cells to maintain the stem cells in an undifferentiated state. It has now been found that high levels of fibroblast growth factor in a medium together with at least one of gamma aminobutyric acid, pipecolic acid, and lithium, enables pluripotent stem cells to remain undifferentiated indefinitely through multiple passages, even without feeder cells or conditioned medium. Without beta-mercaptoethanol, the medium improves cloning efficiency. Also, a matrix of human proteins can be used to culture the undifferentiated cells without exposing the cells to animal products. Further disclosed are new primate pluripotent cell lines made using the defined culture conditions, including the medium and the matrix. Such new cell lines will have never been exposed to animal cells, animal products, feeder cells or conditioned medium.

摘要翻译： 培养灵长类多能干细胞的先前方法需要成纤维细胞饲养细胞或暴露于成纤维细胞饲养细胞的培养基以维持干细胞处于未分化状态。 现在已经发现，培养基中高水平的成纤维细胞生长因子与γ-氨基丁酸，哌啶酸和锂中的至少一种一起使多能干细胞通过多次传代无限期地保持未分化，即使没有饲养细胞或条件培养基 。 没有β-巯基乙醇，培养基提高了克隆效率。 此外，可以使用人类蛋白质的基质来培养未分化细胞而不将细胞暴露于动物产品。 进一步公开的是使用定义的培养条件制备的新的灵长类动物多能细胞系，包括培养基和基质。 这样的新细胞系从未暴露于动物细胞，动物产品，饲养细胞或条件培养基。