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53 条记录 (耗时 0.078 秒)

    Recombinant DNA constructs and methods for controlling gene expression
    33.
    发明授权
    Recombinant DNA constructs and methods for controlling gene expression 有权
    重组DNA构建体和控制基因表达的方法

    公开(公告)号：US08217227B2

    公开(公告)日：2012-07-10

    申请号：US11545072

    申请日：2006-10-10

    申请人： Edwards Allen ,  Larry A. Gilbertson ,  Shihshieh Huang ,  Thomas Malvar ,  James K. Roberts

    发明人： Edwards Allen ,  Larry A. Gilbertson ,  Shihshieh Huang ,  Thomas Malvar ,  James K. Roberts

    IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N15/87 ,  C07H21/04

    CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

    摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

    摘要翻译： 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。

    Methods and DNA constructs for autoregulating transgene silencing
    39.
    发明授权
    Methods and DNA constructs for autoregulating transgene silencing 有权
    用于自转基因沉默的方法和DNA构建体

    公开(公告)号：US09222100B2

    公开(公告)日：2015-12-29

    申请号：US12862078

    申请日：2010-08-24

    申请人： Edwards Allen ,  Larry A. Gilbertson ,  Sergey I. Ivashuta ,  James K. Roberts

    发明人： Edwards Allen ,  Larry A. Gilbertson ,  Sergey I. Ivashuta ,  James K. Roberts

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8218 ,  C12N15/8216 ,  C12N15/8274 ,  C12N15/8285 ,  C12N15/8286

    摘要： This invention provides a method to autoregulate expression of a transgene susceptible to sRNA silencing by concomitantly transcribing RNA from DNA of a transgene and RNA from DNA from at least one sRNA silencing pathway gene. An aspect of the invention provides use of a recombinant DNA construct that includes DNA of a transgene and DNA of an sRNA silencing regulator. Also disclosed are transgenic cells and organisms having in their genome a recombinant DNA construct that includes DNA of a transgene and DNA of an sRNA silencing regulator.

    摘要翻译： 本发明提供了一种通过从来自至少一个sRNA沉默途径基因的DNA的转基因和RNA的DNA同时转录RNA来自动调节对sRNA沉默敏感的转基因的表达的方法。 本发明的一个方面提供了包含sRNA沉默调节子的转基因DNA和DNA的重组DNA构建体的用途。 还公开了在其基因组中具有重组DNA构建体的转基因细胞和生物体，其包含转基因的DNA和sRNA沉默调节子的DNA。