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公开(公告)号：US20120167258A1
公开(公告)日：2012-06-28
申请号：US12665338
申请日：2008-06-26
申请人： Edwards Allen , Sara E. Heisel , Sergey Ivashuta , Elysia Krieger , Jennifer Lutke , Robert J. Meister , Yuanji Zhang
发明人： Edwards Allen , Sara E. Heisel , Sergey Ivashuta , Elysia Krieger , Jennifer Lutke , Robert J. Meister , Yuanji Zhang
CPC分类号： C12N15/8216 , C12N15/8217 , C12N15/8218 , C12N15/8234 , C12N15/8238 , C12N15/8254 , C12N15/8265 , C12N15/827 , C12N15/8275 , C12N15/8279 , C12N15/8285 , C12N15/8286 , C12N15/8289 , Y02A40/162 , Y02A40/164
摘要： This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.
摘要翻译： 本发明提供用于在植物或植物害虫或病原体中时间特异性控制基因表达的分子构建体和方法。 更具体地，本发明提供具有新颖的昼夜节律表达模式的植物miRNA基因，其可用于设计用于至少一种基因的时间特异性表达的重组DNA构建体。 还提供了在其基因组中含有本发明的重组DNA构建体的非天然转基因植物细胞，植物和种子。
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公开(公告)号：US08697949B2
公开(公告)日：2014-04-15
申请号：US12665338
申请日：2008-06-26
申请人： Edwards Allen , Sara E. Heisel , Sergey Ivashuta , Elysia Krieger , Jennifer Lutke , Robert J. Meister , Yuanji Zhang
发明人： Edwards Allen , Sara E. Heisel , Sergey Ivashuta , Elysia Krieger , Jennifer Lutke , Robert J. Meister , Yuanji Zhang
CPC分类号： C12N15/8216 , C12N15/8217 , C12N15/8218 , C12N15/8234 , C12N15/8238 , C12N15/8254 , C12N15/8265 , C12N15/827 , C12N15/8275 , C12N15/8279 , C12N15/8285 , C12N15/8286 , C12N15/8289 , Y02A40/162 , Y02A40/164
摘要： This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.
摘要翻译： 本发明提供用于在植物或植物害虫或病原体中时间特异性控制基因表达的分子构建体和方法。 更具体地，本发明提供具有新颖的昼夜节律表达模式的植物miRNA基因，其可用于设计用于至少一种基因的时间特异性表达的重组DNA构建体。 还提供了在其基因组中含有本发明的重组DNA构建体的非天然转基因植物细胞，植物和种子。
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公开(公告)号：US08404928B2
公开(公告)日：2013-03-26
申请号：US11897611
申请日：2007-08-31
申请人： Edwards Allen , Liang Guo , Sara E. Heisel , Sergey I. Ivashuta , Yuanji Zhang
发明人： Edwards Allen , Liang Guo , Sara E. Heisel , Sergey I. Ivashuta , Yuanji Zhang
CPC分类号： C12N15/113 , C12N15/8218 , C12N15/8245 , C12N15/8254 , C12N15/8266 , C12N15/827
摘要： This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention.
摘要翻译： 本发明公开了编码可用于调节一种或多种感兴趣的基因表达的分阶段的小RNA的重组DNA构建体。 本发明还公开了含有本发明的重组DNA构建体的转基因植物细胞，植物和种子。
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公开(公告)号：US20110035839A1
公开(公告)日：2011-02-10
申请号：US12832942
申请日：2010-07-08
申请人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Leuthy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
发明人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Leuthy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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公开(公告)号：US20090070898A1
公开(公告)日：2009-03-12
申请号：US11974469
申请日：2007-10-12
申请人： Edwards Allen , Barry S. Goldman , Liang Guo , Sara E. Heisel , Shihshieh Huang , Sergey I. Ivashuta , David K. Kovalik , Elysia K. Krieger , James K. Roberts , Yuanji Zhang
发明人： Edwards Allen , Barry S. Goldman , Liang Guo , Sara E. Heisel , Shihshieh Huang , Sergey I. Ivashuta , David K. Kovalik , Elysia K. Krieger , James K. Roberts , Yuanji Zhang
CPC分类号： C12N15/113 , C12N15/8216 , C12N15/8218 , C12N15/8238 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12N15/8286 , C12N2310/141 , C12N2320/50 , Y02A40/146 , Y02A40/162
摘要： This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.
摘要翻译： 本发明公开了新的微小RNA及其前体，以及重组DNA构建体，包括这样的新型miRNA，miRNA前体，miRNA启动子和对应于miRNA的miRNA识别位点。 包括显示营养反应表达的新型miRNA和miRNA前体。 还公开了miRNA诱饵序列。 还提供了非天然转基因植物细胞，植物和种子，在其基因组中含有本发明的重组DNA构建体和使用本发明的重组DNA构建体控制基因表达的方法。
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6.发明授权Engineered miRNA precursors for suppressing target gene expression in plants 有权用于抑制植物中靶基因表达的工程miRNA前体公开(公告)号：US07723568B2
公开(公告)日：2010-05-25
申请号：US11545106
申请日：2006-10-10
申请人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
发明人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
摘要翻译： 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。
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公开(公告)号：US08946511B2
公开(公告)日：2015-02-03
申请号：US11974469
申请日：2007-10-12
申请人： Edwards Allen , Barry S. Goldman , Liang Guo , Sara E. Heisel , Shihshieh Huang , Sergey I. Ivashuta , David K. Kovalik , Elysia K. Krieger , James K. Roberts , Yuanji Zhang
发明人： Edwards Allen , Barry S. Goldman , Liang Guo , Sara E. Heisel , Shihshieh Huang , Sergey I. Ivashuta , David K. Kovalik , Elysia K. Krieger , James K. Roberts , Yuanji Zhang
CPC分类号： C12N15/113 , C12N15/8216 , C12N15/8218 , C12N15/8238 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12N15/8286 , C12N2310/141 , C12N2320/50 , Y02A40/146 , Y02A40/162
摘要： This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.
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公开(公告)号：US20130232644A1
公开(公告)日：2013-09-05
申请号：US13775477
申请日：2013-02-25
申请人： Edwards Allen , Liang Guo , Sara E. Heisel , Sergey I. Ivashuta , Yuanji Zhang
发明人： Edwards Allen , Liang Guo , Sara E. Heisel , Sergey I. Ivashuta , Yuanji Zhang
IPC分类号： C12N15/113
CPC分类号： C12N15/113 , C12N15/8218 , C12N15/8245 , C12N15/8254 , C12N15/8266 , C12N15/827
摘要： This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention
摘要翻译： 本发明公开了编码可用于调节一种或多种感兴趣的基因表达的分阶段的小RNA的重组DNA构建体。 本发明还公开了含有本发明的重组DNA构建体的转基因植物细胞，植物和种子
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9.发明申请公开(公告)号：US20110035838A1
公开(公告)日：2011-02-10
申请号：US12832938
申请日：2010-07-08
申请人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
发明人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
摘要翻译： 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。
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10.发明申请公开(公告)号：US20090013434A1
公开(公告)日：2009-01-08
申请号：US11544953
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael H. Luethy , Philip W. Miller , Larry A. Gilbertson , Edwards Allen , Sara E. Heisel , David K. Kovalic , James K. Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael H. Luethy , Philip W. Miller , Larry A. Gilbertson , Edwards Allen , Sara E. Heisel , David K. Kovalic , James K. Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
摘要翻译： 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。
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