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公开(公告)号：US20090070898A1
公开(公告)日：2009-03-12
申请号：US11974469
申请日：2007-10-12
申请人： Edwards Allen , Barry S. Goldman , Liang Guo , Sara E. Heisel , Shihshieh Huang , Sergey I. Ivashuta , David K. Kovalik , Elysia K. Krieger , James K. Roberts , Yuanji Zhang
发明人： Edwards Allen , Barry S. Goldman , Liang Guo , Sara E. Heisel , Shihshieh Huang , Sergey I. Ivashuta , David K. Kovalik , Elysia K. Krieger , James K. Roberts , Yuanji Zhang
CPC分类号： C12N15/113 , C12N15/8216 , C12N15/8218 , C12N15/8238 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12N15/8286 , C12N2310/141 , C12N2320/50 , Y02A40/146 , Y02A40/162
摘要： This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.
摘要翻译： 本发明公开了新的微小RNA及其前体，以及重组DNA构建体，包括这样的新型miRNA，miRNA前体，miRNA启动子和对应于miRNA的miRNA识别位点。 包括显示营养反应表达的新型miRNA和miRNA前体。 还公开了miRNA诱饵序列。 还提供了非天然转基因植物细胞，植物和种子，在其基因组中含有本发明的重组DNA构建体和使用本发明的重组DNA构建体控制基因表达的方法。
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公开(公告)号：US08946511B2
公开(公告)日：2015-02-03
申请号：US11974469
申请日：2007-10-12
申请人： Edwards Allen , Barry S. Goldman , Liang Guo , Sara E. Heisel , Shihshieh Huang , Sergey I. Ivashuta , David K. Kovalik , Elysia K. Krieger , James K. Roberts , Yuanji Zhang
发明人： Edwards Allen , Barry S. Goldman , Liang Guo , Sara E. Heisel , Shihshieh Huang , Sergey I. Ivashuta , David K. Kovalik , Elysia K. Krieger , James K. Roberts , Yuanji Zhang
CPC分类号： C12N15/113 , C12N15/8216 , C12N15/8218 , C12N15/8238 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12N15/8286 , C12N2310/141 , C12N2320/50 , Y02A40/146 , Y02A40/162
摘要： This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.
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公开(公告)号：US08404928B2
公开(公告)日：2013-03-26
申请号：US11897611
申请日：2007-08-31
申请人： Edwards Allen , Liang Guo , Sara E. Heisel , Sergey I. Ivashuta , Yuanji Zhang
发明人： Edwards Allen , Liang Guo , Sara E. Heisel , Sergey I. Ivashuta , Yuanji Zhang
CPC分类号： C12N15/113 , C12N15/8218 , C12N15/8245 , C12N15/8254 , C12N15/8266 , C12N15/827
摘要： This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention.
摘要翻译： 本发明公开了编码可用于调节一种或多种感兴趣的基因表达的分阶段的小RNA的重组DNA构建体。 本发明还公开了含有本发明的重组DNA构建体的转基因植物细胞，植物和种子。
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公开(公告)号：US20130232644A1
公开(公告)日：2013-09-05
申请号：US13775477
申请日：2013-02-25
申请人： Edwards Allen , Liang Guo , Sara E. Heisel , Sergey I. Ivashuta , Yuanji Zhang
发明人： Edwards Allen , Liang Guo , Sara E. Heisel , Sergey I. Ivashuta , Yuanji Zhang
IPC分类号： C12N15/113
CPC分类号： C12N15/113 , C12N15/8218 , C12N15/8245 , C12N15/8254 , C12N15/8266 , C12N15/827
摘要： This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention
摘要翻译： 本发明公开了编码可用于调节一种或多种感兴趣的基因表达的分阶段的小RNA的重组DNA构建体。 本发明还公开了含有本发明的重组DNA构建体的转基因植物细胞，植物和种子
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公开(公告)号：US20080066206A1
公开(公告)日：2008-03-13
申请号：US11897611
申请日：2007-08-31
申请人： Edwards Allen , Liang Guo , Sara Heisel , Sergey Ivashuta , Yuanji Zhang
发明人： Edwards Allen , Liang Guo , Sara Heisel , Sergey Ivashuta , Yuanji Zhang
CPC分类号： C12N15/113 , C12N15/8218 , C12N15/8245 , C12N15/8254 , C12N15/8266 , C12N15/827
摘要： This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention
摘要翻译： 本发明公开了编码可用于调节一种或多种感兴趣的基因表达的分阶段的小RNA的重组DNA构建体。 本发明还公开了含有本发明的重组DNA构建体的转基因植物细胞，植物和种子
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36.发明申请METHODS FOR GENETIC CONTROL OF PLANT PEST INFESTATION AND COMPOSITIONS THEREOF 有权植物病虫害感染的遗传控制方法及其组合物公开(公告)号：US20090188005A1
公开(公告)日：2009-07-23
申请号：US12101830
申请日：2008-04-11
申请人： Andrey A. Boukharov , Zijin Du , Liang Guo , David K. Kovalic , Maolong Lu , James P. McCarter , Nancy M. Miller , Mark Vaudin , Deryck Jeremy Williams , Wei Wu
发明人： Andrey A. Boukharov , Zijin Du , Liang Guo , David K. Kovalic , Maolong Lu , James P. McCarter , Nancy M. Miller , Mark Vaudin , Deryck Jeremy Williams , Wei Wu
CPC分类号： C12N15/8285 , C07K14/4354 , C12N15/8241 , C12N15/8245 , C12N15/8247 , C12N15/8274 , C12N15/8279 , Y02A40/164
摘要： The present invention is directed to controlling plant pest infestation, and particularly plant nematode infestation, by inhibiting one or more biological functions in the plant pest. The invention discloses methods and compositions for use in controlling plant pest infestation by providing one or more different recombinant double stranded RNA molecules in the diet of the pest in order to achieve a reduction in pest infestation through suppression of pest gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, to methods for detecting cells comprising the disclosed sequences, and to methods for detecting the disclosed sequences in biological samples.
摘要翻译： 本发明涉及通过抑制植物有害生物中的一种或多种生物功能来控制植物害虫侵染，特别是植物线虫侵染。 本发明公开了通过在害虫的饮食中提供一种或多种不同的重组双链RNA分子来控制植物害虫侵袭的方法和组合物，以通过抑制害虫基因表达来实现害虫侵染的减少。 本发明还涉及用于制备表达双链RNA分子的转基因植物的方法，用于检测包含所公开序列的细胞的方法，以及用于检测生物样品中所公开序列的方法。
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37.发明申请Methods for genetic control of plant pest infestation and compositions thereof 审中-公开植物害虫侵染遗传控制方法及其组成公开(公告)号：US20140325702A1
公开(公告)日：2014-10-30
申请号：US13374480
申请日：2011-12-29
申请人： Andrey A. Boukharov , Zijin Du , Liang Guo , David K. Kovalic , Maolong Lu , James P. McCarter , Nancy M. Miller , Mark Vaudin , Deryck Jeremy Williams , Wei Wu
发明人： Andrey A. Boukharov , Zijin Du , Liang Guo , David K. Kovalic , Maolong Lu , James P. McCarter , Nancy M. Miller , Mark Vaudin , Deryck Jeremy Williams , Wei Wu
IPC分类号： C12N15/82
CPC分类号： C12N15/8285 , C07K14/4354 , C12N15/8241 , C12N15/8245 , C12N15/8247 , C12N15/8274 , C12N15/8279 , Y02A40/164
摘要： The present invention is directed to controlling plant pest infestation, and particularly plant nematode infestation, by inhibiting one or more biological functions in the plant pest. The invention discloses methods and compositions for use in controlling plant pest infestation by providing one or more different recombinant double stranded RNA molecules in the diet of the pest in order to achieve a reduction in pest infestation through suppression of pest gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, to methods for detecting cells comprising the disclosed sequences, and to methods for detecting the disclosed sequences in biological samples.
摘要翻译： 本发明涉及通过抑制植物有害生物中的一种或多种生物功能来控制植物害虫侵染，特别是植物线虫侵染。 本发明公开了通过在害虫的饮食中提供一种或多种不同的重组双链RNA分子来控制植物害虫侵袭的方法和组合物，以通过抑制害虫基因表达来实现害虫侵染的减少。 本发明还涉及用于制备表达双链RNA分子的转基因植物的方法，用于检测包含所公开序列的细胞的方法，以及用于检测生物样品中所公开序列的方法。
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38.发明授权公开(公告)号：US08519225B2
公开(公告)日：2013-08-27
申请号：US11673351
申请日：2007-02-09
申请人： Andrey A. Boukharov , Zijin Du , Liang Guo , Michelle C. Hresko , David K. Kovalic , Zhaolong Li , Maolong Lu , James P. McCarter , Nancy M. Miller , Mark Vaudin , Deryck J. Williams , Wei Wu
发明人： Andrey A. Boukharov , Zijin Du , Liang Guo , Michelle C. Hresko , David K. Kovalic , Zhaolong Li , Maolong Lu , James P. McCarter , Nancy M. Miller , Mark Vaudin , Deryck J. Williams , Wei Wu
CPC分类号： C12N15/8285 , A01N57/16 , A23L33/105 , A23V2002/00 , A61K31/713 , C07K14/435 , C07K14/4354 , C12N15/113 , C12N15/1137 , C12N15/8261 , C12N15/8273 , C12N2310/11 , C12Q1/6888 , Y02A40/164
摘要： The invention relates to identifying and evaluating target coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.
摘要翻译： 本发明涉及鉴定和评估通过抑制一种或多种生物学功能及其用途来控制植物寄生线虫的靶编码序列。 本发明提供用于鉴定这种序列并控制植物寄生线虫种群的方法和组合物。 通过将本发明提供的一种或多种重组双链RNA分子送入线虫，通过抑制线虫基因表达可以获得疾病的减少。 本发明还涉及用于制备表达双链RNA分子的转基因植物以及由此获得的植物细胞和植物的方法。
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39.发明授权Isolated novel acid and protein molecules from soy and methods of using those molecules to generate transgene plants with enhanced agronomic traits 有权来自大豆的分离的新型核酸和蛋白质分子以及使用这些分子产生具有增强的农学性状的转基因植物的方法公开(公告)号：US09012723B2
公开(公告)日：2015-04-21
申请号：US13138181
申请日：2010-01-13
申请人： Liang Guo , David K. Kovalic , Bo-Xing Qiu , Jack E. Tabaska , Wei Wu
发明人： Liang Guo , David K. Kovalic , Bo-Xing Qiu , Jack E. Tabaska , Wei Wu
CPC分类号： C12N15/8251 , A01H1/04 , C07K14/415 , C12N15/8247 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8275 , Y02A40/146
摘要： This disclosure provides purified nucleic acids and polypeptides. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants.
摘要翻译： 本公开提供了纯化的核酸和多肽。 还提供了转基因植物，种子和含有用于表达可用于赋予转基因作物植物增强的农学性状的蛋白质的DNA的植物细胞，以及制造这种植物的方法以及制造包括种子和杂交种子在内的农产品的方法 从这样的植物。
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40.发明申请ISOLATED NOVEL ACID AND PROTEIN MOLECULES FROM SOY AND METHODS OF USING THOSE MOLECULES TO GENERATE TRANSGENE PLANTS WITH ENHANCED AGRONOMIC TRAITS 有权来自大豆的分离的新型核酸和蛋白质分子以及使用这些分子生产具有增强的农业条件的转基因植物的方法公开(公告)号：US20120246748A1
公开(公告)日：2012-09-27
申请号：US13138181
申请日：2010-01-13
申请人： Liang Guo , David K. Kovalic , Bo-Xing Qiu , Jack E. Tabaska , Wei Wu
发明人： Liang Guo , David K. Kovalic , Bo-Xing Qiu , Jack E. Tabaska , Wei Wu
CPC分类号： C12N15/8251 , A01H1/04 , C07K14/415 , C12N15/8247 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8275 , Y02A40/146
摘要： This disclosure provides purified nucleic acids and polypeptides. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants.
摘要翻译： 本公开提供了纯化的核酸和多肽。 还提供了转基因植物，种子和含有用于表达可用于赋予转基因作物植物增强的农学性状的蛋白质的DNA的植物细胞，以及制造这种植物的方法以及制造包括种子和杂交种子在内的农产品的方法 从这样的植物。
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