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    Recombinant DNA constructs and methods for controlling gene expression
    36.
    发明授权
    Recombinant DNA constructs and methods for controlling gene expression 有权
    重组DNA构建体和控制基因表达的方法

    公开(公告)号：US08217227B2

    公开(公告)日：2012-07-10

    申请号：US11545072

    申请日：2006-10-10

    申请人： Edwards Allen ,  Larry A. Gilbertson ,  Shihshieh Huang ,  Thomas Malvar ,  James K. Roberts

    发明人： Edwards Allen ,  Larry A. Gilbertson ,  Shihshieh Huang ,  Thomas Malvar ,  James K. Roberts

    IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N15/87 ,  C07H21/04

    CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

    摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

    摘要翻译： 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。