Mannanases
    32.
    发明授权
    Mannanases 有权
    甘露聚糖酶

    公开(公告)号:US06566114B1

    公开(公告)日:2003-05-20

    申请号:US09339159

    申请日:1999-06-24

    IPC分类号: C12N900

    摘要: Novel mannanases comprising e.g. an amino acid sequence as shown in positions 31-330 of SEQ ID NO:2 or their homologues may be derived from e.g. Bacillus sp. I633, or may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO: 1 from nucleotide 91 to nucleotide 990, polynucleotide molecules that encode a polypeptide that is at least 65% identical to the amino acid sequence of SEQ ID NO: 2 from amino acid residue 31 to amino acid residue 330, or degenerate nucleotide sequences thereof. The mannanases are alkaline and are useful e.g. in cleaning compositions, in a fracturing fluid useful to fracture a subterranean formation, for modifying plant material, and for treatment of cellulosic fibers.

    摘要翻译: 新型甘露聚糖酶 SEQ ID NO:2的位置31-330所示的氨基酸序列或其同源物可以衍生自例如SEQ ID NO: 芽孢杆菌 I633,或者可以由包含核苷酸91至核苷酸990的SEQ ID NO:1所示的核苷酸序列的多核苷酸分子编码,编码与SEQ ID NO的氨基酸序列至少65%相同的多肽的多核苷酸分子 :2个氨基酸残基31至氨基酸残基330,或其简并核苷酸序列。 甘露聚糖酶是碱性的,并且是有用的。 在清洁组合物中,在用于破坏地层的压裂液中,用于修饰植物材料和用于纤维素纤维的处理。

    Enzyme with .beta.-(1-6)- endoglucanase activity
    37.
    发明授权
    Enzyme with .beta.-(1-6)- endoglucanase activity 失效
    具有β-(1-6) - 内切葡聚糖酶活性的酶

    公开(公告)号:US6022723A

    公开(公告)日:2000-02-08

    申请号:US40699

    申请日:1998-03-18

    摘要: The present invention relates to an enzyme with .beta.-(1-6)-endoglucanase activity encoded by a DNA sequence, which DNA sequence a) comprises the DNA sequence shown in SEQ ID No. 3, or b) comprises an analogue of the DNA sequence shown in SEQ ID No. 3, which i) is homologous with the DNA sequences shown in or SEQ ID No. 3, and/or ii) hybridizes with the same oligonucleotide probe as the DNA sequence shown in SEQ ID No. 3, and/or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence shown in SEQ ID No. 3, and/or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against a purified .beta.-(1-6)-glucanase shown in SEQ ID No. 4 derived from Trichoderma harzianum, CBS 243.71. The present invention also relates to a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme preparation containing the enzyme, and the use of the enzyme and enzyme preparation for the degradation or modification of .beta.-glucan containing materials.

    摘要翻译: 本发明涉及由DNA序列编码的β-(1-6) - 内葡聚糖酶活性的酶,该DNA序列a)包含SEQ ID No.3所示的DNA序列,或b)包含DNA的类似物 序列号3所示的序列,其i)与SEQ ID No.3所示的DNA序列同源,和/或ii)与与SEQ ID No.3所示的DNA序列相同的寡核苷酸探针杂交, 和/或iii)编码与由包含SEQ ID No.3所示的DNA序列的DNA序列编码的多肽同源的多肽,和/或iv)编码与针对纯化的抗体产生的抗体具有免疫反应性的多肽 β-(1-6) - 葡聚糖酶,如SEQ ID No.4所示,源自哈茨木霉,CBS 243.71。 本发明还涉及编码该酶的DNA构建体,该酶的制备方法,含该酶的酶制剂,以及该酶和酶制剂用于含β-葡聚糖的材料的降解或修饰的用途。

    Endoglucanases
    38.
    发明申请
    Endoglucanases 审中-公开

    公开(公告)号:US20080145912A1

    公开(公告)日:2008-06-19

    申请号:US11740076

    申请日:2007-04-25

    摘要: The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1   2   3   4   5   6   7   8   9   10  11  12 Trp Xaa 13  14 and a second amino acid sequence having the following sequence Trp Cys Cys Xaa Cys(SEQ ID NO: 80) 1   2   3   4   5 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

    Mannanases
    39.
    发明授权
    Mannanases 有权
    甘露聚糖酶

    公开(公告)号:US07183093B2

    公开(公告)日:2007-02-27

    申请号:US10372054

    申请日:2003-02-20

    摘要: The present invention relates to mannanases comprising e.g., a sequence of amino acids 32–330 of SEQ ID NO: 2 or their homologues may be derived from e.g., Bacillus sp. I633, or may be encoded by polynucleotide molecules comprising a sequence of nucleotides 94–990 of SEQ ID NO: 1 from, polynucleotide molecules that encode a polypeptide that is at least 65% identical to the sequence of amino acids 32–330 of SEQ ID NO: 2, or degenerate nucleotide sequences thereof. The mannanases are alkaline and are useful e.g. in cleaning compositions, in a fracturing fluid useful to fracture a subterranean formation, for modifying plant material, and for treatment of cellulosic fibers.

    摘要翻译: 本发明涉及包含例如SEQ ID NO:2的氨基酸32-330的序列的甘露聚糖酶或其同源物可以衍生自例如Bacillus sp。 或者可以由包含SEQ ID NO:1的核苷酸94-990的序列的多核苷酸分子编码的多核苷酸分子编码多肽分子,所述多核苷酸分子编码与SEQ ID NO:32的氨基酸32-330的序列至少65%相同的多肽 NO:2或其简并核苷酸序列。 甘露聚糖酶是碱性的,并且是有用的。 在清洁组合物中,在用于破坏地层的压裂液中,用于修饰植物材料和用于纤维素纤维的处理。