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    Endoglucanases
    2.
    发明授权
    Endoglucanases 失效
    内切葡聚糖酶

    公开(公告)号：US6001639A

    公开(公告)日：1999-12-14

    申请号：US651136

    申请日：1996-05-21

    申请人： Martin Schulein ,  Lene Nonboe Andersen ,  S.o slashed.ren Flensted Lassen ,  Markus Sakari Kauppinen ,  Lene Lange ,  Ruby Iium Nielsen ,  Michiko Ihara ,  Shinobu Takagi

    发明人： Martin Schulein ,  Lene Nonboe Andersen ,  S.o slashed.ren Flensted Lassen ,  Markus Sakari Kauppinen ,  Lene Lange ,  Ruby Iium Nielsen ,  Michiko Ihara ,  Shinobu Takagi

    IPC分类号： C12N15/09 ,  C05F11/08 ,  C05F17/00 ,  C07G99/00 ,  C11D3/386 ,  C12N1/19 ,  C12N9/42 ,  C12N15/00 ,  C12R1/645 ,  C12R1/69 ,  C12R1/77 ,  C12R1/80 ,  C12S3/04 ,  C12S11/00 ,  D06M13/02 ,  D06M13/322 ,  D06M13/46 ,  D06M16/00 ,  D06P5/02 ,  D06P5/15 ,  D21C5/00 ,  D21H11/16 ,  D21H11/20 ,  D21H17/00 ,  D21H21/10 ,  D21C1/00

    CPC分类号： C12N9/2437 ,  A61K38/00 ,  C05F11/08 ,  C05F17/0036 ,  C11D3/386 ,  C12P19/14 ,  C12Y302/01004 ,  D06M16/003 ,  D06P5/02 ,  D06P5/158 ,  D21C5/005 ,  D21H21/10 ,  C07K2319/00 ,  D21H17/005 ,  Y02P20/145 ,  Y02W30/43

    摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence consisting of 14 amino acid residues having the following sequenceThr Arg Xaa Xaa Asp Cys Cys Xaa Xaa (SEQ ID NO:79) 1 2 3 4 5 6 7 8 9 - Xaa Cys Xaa Trp Xaa 10 11 12 13 14 and a second amino acid sequence consisting of 5 amino acid residues having the following sequenceTrp Cys Cys Xaa Cys (SEQ ID NO:80) 1 2 3 4 5 wherein, in position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 8 of the first sequence, the amino acid is Arg, Lys or His; in position 9, 10, 12 and 14, respectively, of the first sequence, and in position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue in position 12 is Ser, then the amino acid residue in position 14 is not Ser, and (ii) when the amino residue in position 12 is Gly, then the amino acid residue in position 14 is not Ala, performs very good in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

    摘要翻译： 本发明涉及基本上由具有纤维素分解活性的酶组成的酶制剂，其包含由具有以下序列的14个氨基酸残基组成的第一氨基酸序列：Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa（SEQ ID NO：79） - 1 2 3 4 5 6 7 8 9 - - Xaa Cys Xaa Trp Xaa - 10 11 12 13 14 - 和由具有以下序列的5个氨基酸残基组成的第二个氨基酸序列--Trp Cys Cys Xaa Cys（SEQ ID NO ：80） - 1 2 3 4 5 - 其中，在第一序列的位置3，氨基酸是Trp，Tyr或Phe; 在第一序列的位置4，氨基酸是Trp，Tyr或Phe; 在第一序列的位置8，氨基酸是Arg，Lys或His; 分别位于第一序列的第9,10,12和14位，在第二序列的位置4中，氨基酸是20个天然存在的氨基酸残基中的任一个，条件是在第一个氨基酸序列 ，（i）当位置12的氨基残基为Ser时，14位的氨基酸残基不为Ser，（ii）当位置12的氨基残基为Gly时，位置14的氨基酸残基为 Ala在工业应用中表现非常好，例如洗衣组合物，用于生物抛光新制造的纺织品，用于提供纤维素织物或服装的磨损外观以及纸浆处理。 此外，本发明涉及编码这些酶的DNA构建体，提供编码这种酶的基因的方法，生产酶的方法，含有这些酶的酶制剂，以及这些酶用于许多工业应用的用途。

    Endoglucanases
    3.
    发明申请
    Endoglucanases 审中-公开

    公开(公告)号：US20080145912A1

    公开(公告)日：2008-06-19

    申请号：US11740076

    申请日：2007-04-25

    申请人： Martin Schulein ,  Torben Henriksen ,  Lene Nonboe Andersen ,  Soren Flensted Lassen ,  Markus Sakari Kauppinen ,  Lene Lange ,  Ruby Ilum Nielsen ,  Shinobu Takagi ,  Michiko Ihara

    发明人： Martin Schulein ,  Torben Henriksen ,  Lene Nonboe Andersen ,  Soren Flensted Lassen ,  Markus Sakari Kauppinen ,  Lene Lange ,  Ruby Ilum Nielsen ,  Shinobu Takagi ,  Michiko Ihara

    IPC分类号： C12N9/18 ,  C07H21/04 ,  C12N15/00 ,  D06M16/00

    摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1   2   3   4   5   6   7   8   9   10  11  12 Trp Xaa 13  14 and a second amino acid sequence having the following sequence Trp Cys Cys Xaa Cys(SEQ ID NO: 80) 1   2   3   4   5 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

    Mannanases
    4.
    发明授权
    Mannanases 有权
    甘露聚糖酶

    公开(公告)号：US07183093B2

    公开(公告)日：2007-02-27

    申请号：US10372054

    申请日：2003-02-20

    申请人： Markus Sakari Kauppinen ,  Martin Schulein ,  Kirk Schnorr ,  Lene Nonboe Andersen ,  Mads Eskelund Bjornvad

    发明人： Markus Sakari Kauppinen ,  Martin Schulein ,  Kirk Schnorr ,  Lene Nonboe Andersen ,  Mads Eskelund Bjornvad

    IPC分类号： C12N9/42 ,  C12N1/20 ,  C12P19/64 ,  C12P19/34 ,  C12P19/04 ,  D06M16/00

    CPC分类号： C12N9/2491 ,  C12Y302/01025 ,  C12Y302/01078

    摘要： The present invention relates to mannanases comprising e.g., a sequence of amino acids 32–330 of SEQ ID NO: 2 or their homologues may be derived from e.g., Bacillus sp. I633, or may be encoded by polynucleotide molecules comprising a sequence of nucleotides 94–990 of SEQ ID NO: 1 from, polynucleotide molecules that encode a polypeptide that is at least 65% identical to the sequence of amino acids 32–330 of SEQ ID NO: 2, or degenerate nucleotide sequences thereof. The mannanases are alkaline and are useful e.g. in cleaning compositions, in a fracturing fluid useful to fracture a subterranean formation, for modifying plant material, and for treatment of cellulosic fibers.

    摘要翻译： 本发明涉及包含例如SEQ ID NO：2的氨基酸32-330的序列的甘露聚糖酶或其同源物可以衍生自例如Bacillus sp。 或者可以由包含SEQ ID NO：1的核苷酸94-990的序列的多核苷酸分子编码的多核苷酸分子编码多肽分子，所述多核苷酸分子编码与SEQ ID NO：32的氨基酸32-330的序列至少65％相同的多肽 NO：2或其简并核苷酸序列。 甘露聚糖酶是碱性的，并且是有用的。 在清洁组合物中，在用于破坏地层的压裂液中，用于修饰植物材料和用于纤维素纤维的处理。

    Xyloglucanase from Malbranchea
    5.
    发明授权
    Xyloglucanase from Malbranchea 失效
    来自马尔布兰卡的葡聚糖酶

    公开(公告)号：US06500658B1

    公开(公告)日：2002-12-31

    申请号：US09653778

    申请日：2000-09-01

    申请人： Wenping Wu ,  Martin Schulein ,  Markus Sakari Kauppinen ,  Mary Ann Stringer

    发明人： Wenping Wu ,  Martin Schulein ,  Markus Sakari Kauppinen ,  Mary Ann Stringer

    IPC分类号： C12P2106

    CPC分类号： D06M16/003 ,  C11D3/38636 ,  D06L4/40

    摘要： An isolated or purified polypeptide having xyloglucanase activity which is obtained from a strain of the genus Malbranchea and has xyloglucanase activity in the pH range 4-11, measured at 50° C.; and/or a molecular mass of 25±10 kDa, as determined by SDS-PAGE; and/or an isoelectric point (pI) in the range 3-5; and/or an N-terminal sequence Ala-Asp-Phe-Cys-Gly-Gln-Xaa-Asp-Ser-Glu-Gln-Ser-Gly-Pro-Tyr-Ile-Val-Tyr-Asn-Asn-Leu is useful in industrial applications such as in laundry detergent compositions and for treatment of textiles.

    摘要翻译： 具有木葡聚糖酶活性的分离的或纯化的多肽，其从马氏体属的菌株获得，并且在50℃下测量的pH范围4-11中具有木葡聚糖酶活性。 和/或25±10kDa的分子量，通过SDS-PAGE测定; 和/或3-5的等电点（pI）; 和/或N-末端序列Ala-Asp-Phe-Cys-Gly-Gln-Xaa-Asp-Ser-Glu-Gln-Ser-Gly-Pro-Tyr-Ile-Val-Tyr-Asn-Asn-Leu是 可用于工业应用，例如洗衣洗涤剂组合物和用于纺织品的处理。

    Endoglucanases
    8.
    发明授权
    Endoglucanases 失效
    内切葡聚糖酶

    公开(公告)号：US08642730B2

    公开(公告)日：2014-02-04

    申请号：US12567302

    申请日：2009-09-25

    申请人： Lene Nonboe Andersen ,  Soren Flensted Lassen ,  Markus Sakari Kauppinen ,  Lene Lange ,  Ruby Ilum Nielsen ,  Shinobu Takagi ,  Michiko Ihara ,  Torben Henriksen

    发明人： Martin Schulein ,  Lene Nonboe Andersen ,  Soren Flensted Lassen ,  Markus Sakari Kauppinen ,  Lene Lange ,  Ruby Ilum Nielsen ,  Shinobu Takagi ,  Michiko Ihara

    IPC分类号： C07K1/00

    CPC分类号： C12N9/2437 ,  A61K38/00 ,  C05F11/08 ,  C05F17/0036 ,  C07K2319/00 ,  C11D3/386 ,  C12P19/14 ,  C12Y302/01004 ,  D06M16/003 ,  D06P5/02 ,  D06P5/158 ,  D21C5/005 ,  D21H17/005 ,  D21H21/10 ,  Y02P20/145 ,  Y02W30/43

    摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1   2   3   4   5   6   7   8   9   10  11  12 Trp Xaa 13  14 and a second amino acid sequence having the following sequence Trp Cys Cys Xaa Cys(SEQ ID NO: 80) 1   2   3   4   5 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

    摘要翻译： 本发明涉及基本上由具有纤维素分解活性的酶组成并包含具有以下序列的第一氨基酸序列的酶制剂（SEQ ID NO：79）Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 123456789101112 Trp Xaa 1314 和具有以下序列的第二氨基酸序列Trp Cys Cys Xaa Cys（SEQ ID NO：80）12345其中，在第一序列的3位，氨基酸为Trp，Tyr或Phe; 在第一序列的位置4处，氨基酸是Trp，Tyr或Phe; 在第一序列的位置8，氨基酸是Arg，Lys或His; 在第一序列的第9,10,12和14位，第2个序列的第4位，氨基酸是20个天然存在的氨基酸残基中的任一个，条件是在第一个氨基酸序列 ，（i）当第12位的氨基残基为Ser时，第14位的氨基酸残基不为Ser，（ii）当第12位的氨基残基为Gly时，第14位的氨基酸残基为 Ala在工业应用中表现良好，例如洗衣组合物，用于生物抛光新制造的纺织品，用于提供纤维素织物或衣服的磨损外观以及纸浆处理。 此外，本发明涉及编码这些酶的DNA构建体，提供编码这种酶的基因的方法，生产酶的方法，含有这些酶的酶制剂，以及这些酶用于许多工业应用的用途。