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公开(公告)号:US07183093B2
公开(公告)日:2007-02-27
申请号:US10372054
申请日:2003-02-20
申请人: Markus Sakari Kauppinen , Martin Schulein , Kirk Schnorr , Lene Nonboe Andersen , Mads Eskelund Bjornvad
发明人: Markus Sakari Kauppinen , Martin Schulein , Kirk Schnorr , Lene Nonboe Andersen , Mads Eskelund Bjornvad
CPC分类号: C12N9/2491 , C12Y302/01025 , C12Y302/01078
摘要: The present invention relates to mannanases comprising e.g., a sequence of amino acids 32–330 of SEQ ID NO: 2 or their homologues may be derived from e.g., Bacillus sp. I633, or may be encoded by polynucleotide molecules comprising a sequence of nucleotides 94–990 of SEQ ID NO: 1 from, polynucleotide molecules that encode a polypeptide that is at least 65% identical to the sequence of amino acids 32–330 of SEQ ID NO: 2, or degenerate nucleotide sequences thereof. The mannanases are alkaline and are useful e.g. in cleaning compositions, in a fracturing fluid useful to fracture a subterranean formation, for modifying plant material, and for treatment of cellulosic fibers.
摘要翻译: 本发明涉及包含例如SEQ ID NO:2的氨基酸32-330的序列的甘露聚糖酶或其同源物可以衍生自例如Bacillus sp。 或者可以由包含SEQ ID NO:1的核苷酸94-990的序列的多核苷酸分子编码的多核苷酸分子编码多肽分子,所述多核苷酸分子编码与SEQ ID NO:32的氨基酸32-330的序列至少65%相同的多肽 NO:2或其简并核苷酸序列。 甘露聚糖酶是碱性的,并且是有用的。 在清洁组合物中,在用于破坏地层的压裂液中,用于修饰植物材料和用于纤维素纤维的处理。
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公开(公告)号:US06677147B2
公开(公告)日:2004-01-13
申请号:US10072152
申请日:2002-02-07
申请人: Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schroder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
发明人: Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schroder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
IPC分类号: C12N988
CPC分类号: C12N9/2465 , A23K10/14 , A23K20/189 , C07K2319/20 , C11D3/386 , C12N9/88 , D06L4/40 , D06M16/003 , D21C5/005 , Y10T442/20
摘要: The present invention relates to pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) (SEQ ID NO: 2) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase may be derivable from Bacillus species.
摘要翻译: 本发明涉及属于多糖裂解酶1族的氨基酸序列Asn Leu Asn Ser Arg Val Pro(NLNSRVP)(SEQ ID NO:2)的果胶酸裂解酶在中性或碱性条件下的工业过程中具有良好的性能,例如洗涤 和纺织加工。 果胶裂解酶可以衍生自芽孢杆菌属物种。
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公开(公告)号:US06368843B1
公开(公告)日:2002-04-09
申请号:US09694531
申请日:2000-10-23
申请人: Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schrøder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
发明人: Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schrøder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
IPC分类号: C12N926
CPC分类号: C12N9/2465 , A23K10/14 , A23K20/189 , C07K2319/20 , C11D3/386 , C12N9/88 , D06L4/40 , D06M16/003 , D21C5/005 , Y10T442/20
摘要: A novel group of pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase may be derivable from Bacillus species.
摘要翻译: 属于多糖裂解酶1族的氨基酸序列Asn Leu Asn Ser Arg Val Pro(NLNSRVP)的新一组果胶酸裂解酶在中性或碱性条件例如洗涤和纺织加工中在工业过程中具有良好的性能。 果胶裂解酶可以衍生自芽孢杆菌属物种。
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公开(公告)号:US06187580B1
公开(公告)日:2001-02-13
申请号:US09198955
申请日:1998-11-24
申请人: Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schrøder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
发明人: Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schrøder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
IPC分类号: C12N988
CPC分类号: C12N9/2465 , A23K10/14 , A23K20/189 , C07K2319/20 , C11D3/386 , C12N9/88 , D06L4/40 , D06M16/003 , D21C5/005 , Y10T442/20
摘要: A novel group of pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase are derivable from Bacillus species.
摘要翻译: 属于多糖裂解酶1族的氨基酸序列Asn Leu Asn Ser Arg Val Pro(NLNSRVP)的新一组果胶酸裂解酶在中性或碱性条件例如洗涤和纺织加工中在工业过程中具有良好的性能。 果胶裂解酶可从芽孢杆菌属物种衍生。
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公开(公告)号:US06566114B1
公开(公告)日:2003-05-20
申请号:US09339159
申请日:1999-06-24
申请人: Markus Sakari Kauppinen , Martin Schülein , Kirk Schnorr , Lene Nonboe Andersen , Mads Eskelund Bjørnvad
发明人: Markus Sakari Kauppinen , Martin Schülein , Kirk Schnorr , Lene Nonboe Andersen , Mads Eskelund Bjørnvad
IPC分类号: C12N900
CPC分类号: C12N9/2491 , C12Y302/01025 , C12Y302/01078
摘要: Novel mannanases comprising e.g. an amino acid sequence as shown in positions 31-330 of SEQ ID NO:2 or their homologues may be derived from e.g. Bacillus sp. I633, or may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO: 1 from nucleotide 91 to nucleotide 990, polynucleotide molecules that encode a polypeptide that is at least 65% identical to the amino acid sequence of SEQ ID NO: 2 from amino acid residue 31 to amino acid residue 330, or degenerate nucleotide sequences thereof. The mannanases are alkaline and are useful e.g. in cleaning compositions, in a fracturing fluid useful to fracture a subterranean formation, for modifying plant material, and for treatment of cellulosic fibers.
摘要翻译: 新型甘露聚糖酶 SEQ ID NO:2的位置31-330所示的氨基酸序列或其同源物可以衍生自例如SEQ ID NO: 芽孢杆菌 I633,或者可以由包含核苷酸91至核苷酸990的SEQ ID NO:1所示的核苷酸序列的多核苷酸分子编码,编码与SEQ ID NO的氨基酸序列至少65%相同的多肽的多核苷酸分子 :2个氨基酸残基31至氨基酸残基330,或其简并核苷酸序列。 甘露聚糖酶是碱性的,并且是有用的。 在清洁组合物中,在用于破坏地层的压裂液中,用于修饰植物材料和用于纤维素纤维的处理。
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公开(公告)号:US6001639A
公开(公告)日:1999-12-14
申请号:US651136
申请日:1996-05-21
申请人: Martin Schulein , Lene Nonboe Andersen , S.o slashed.ren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Iium Nielsen , Michiko Ihara , Shinobu Takagi
发明人: Martin Schulein , Lene Nonboe Andersen , S.o slashed.ren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Iium Nielsen , Michiko Ihara , Shinobu Takagi
IPC分类号: C12N15/09 , C05F11/08 , C05F17/00 , C07G99/00 , C11D3/386 , C12N1/19 , C12N9/42 , C12N15/00 , C12R1/645 , C12R1/69 , C12R1/77 , C12R1/80 , C12S3/04 , C12S11/00 , D06M13/02 , D06M13/322 , D06M13/46 , D06M16/00 , D06P5/02 , D06P5/15 , D21C5/00 , D21H11/16 , D21H11/20 , D21H17/00 , D21H21/10 , D21C1/00
CPC分类号: C12N9/2437 , A61K38/00 , C05F11/08 , C05F17/0036 , C11D3/386 , C12P19/14 , C12Y302/01004 , D06M16/003 , D06P5/02 , D06P5/158 , D21C5/005 , D21H21/10 , C07K2319/00 , D21H17/005 , Y02P20/145 , Y02W30/43
摘要: The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence consisting of 14 amino acid residues having the following sequenceThr Arg Xaa Xaa Asp Cys Cys Xaa Xaa (SEQ ID NO:79) 1 2 3 4 5 6 7 8 9 - Xaa Cys Xaa Trp Xaa 10 11 12 13 14 and a second amino acid sequence consisting of 5 amino acid residues having the following sequenceTrp Cys Cys Xaa Cys (SEQ ID NO:80) 1 2 3 4 5 wherein, in position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 8 of the first sequence, the amino acid is Arg, Lys or His; in position 9, 10, 12 and 14, respectively, of the first sequence, and in position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue in position 12 is Ser, then the amino acid residue in position 14 is not Ser, and (ii) when the amino residue in position 12 is Gly, then the amino acid residue in position 14 is not Ala, performs very good in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
摘要翻译: 本发明涉及基本上由具有纤维素分解活性的酶组成的酶制剂,其包含由具有以下序列的14个氨基酸残基组成的第一氨基酸序列:Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa(SEQ ID NO:79) - 1 2 3 4 5 6 7 8 9 - - Xaa Cys Xaa Trp Xaa - 10 11 12 13 14 - 和由具有以下序列的5个氨基酸残基组成的第二个氨基酸序列--Trp Cys Cys Xaa Cys(SEQ ID NO :80) - 1 2 3 4 5 - 其中,在第一序列的位置3,氨基酸是Trp,Tyr或Phe; 在第一序列的位置4,氨基酸是Trp,Tyr或Phe; 在第一序列的位置8,氨基酸是Arg,Lys或His; 分别位于第一序列的第9,10,12和14位,在第二序列的位置4中,氨基酸是20个天然存在的氨基酸残基中的任一个,条件是在第一个氨基酸序列 ,(i)当位置12的氨基残基为Ser时,14位的氨基酸残基不为Ser,(ii)当位置12的氨基残基为Gly时,位置14的氨基酸残基为 Ala在工业应用中表现非常好,例如洗衣组合物,用于生物抛光新制造的纺织品,用于提供纤维素织物或服装的磨损外观以及纸浆处理。 此外,本发明涉及编码这些酶的DNA构建体,提供编码这种酶的基因的方法,生产酶的方法,含有这些酶的酶制剂,以及这些酶用于许多工业应用的用途。
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公开(公告)号:US07226773B2
公开(公告)日:2007-06-05
申请号:US10965499
申请日:2004-10-14
申请人: Martin Schulein, deceased , Torben Henriksen, legal representative , Lene Nonboe Andersen , Soren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Ilum Nielsen , Shinobu Takagi , Michiko Ihara
发明人: Martin Schulein, deceased , Torben Henriksen, legal representative , Lene Nonboe Andersen , Soren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Ilum Nielsen , Shinobu Takagi , Michiko Ihara
CPC分类号: C12N9/2437 , A61K38/00 , C05F11/08 , C05F17/0036 , C07K2319/00 , C11D3/386 , C12P19/14 , C12Y302/01004 , D06M16/003 , D06P5/02 , D06P5/158 , D21C5/005 , D21H17/005 , D21H21/10 , Y02P20/145 , Y02W30/43
摘要: The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) ThrArgXaaXaaAspCysCysXaaXaaXaaCysXaaTrpXaa 1234567891011121314 and a second amino acid sequence having the following sequence TrpCysCysXaaCys(SEQ ID NO: 80) 12345 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
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公开(公告)号:US20080145912A1
公开(公告)日:2008-06-19
申请号:US11740076
申请日:2007-04-25
申请人: Martin Schulein , Torben Henriksen , Lene Nonboe Andersen , Soren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Ilum Nielsen , Shinobu Takagi , Michiko Ihara
发明人: Martin Schulein , Torben Henriksen , Lene Nonboe Andersen , Soren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Ilum Nielsen , Shinobu Takagi , Michiko Ihara
摘要: The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1 2 3 4 5 6 7 8 9 10 11 12 Trp Xaa 13 14 and a second amino acid sequence having the following sequence Trp Cys Cys Xaa Cys(SEQ ID NO: 80) 1 2 3 4 5 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
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公开(公告)号:US6071735A
公开(公告)日:2000-06-06
申请号:US956253
申请日:1997-10-22
申请人: Martin Schulein , Karen Margrethe Oxenb.o slashed.ll , Lene Nonboe Andersen , S.o slashed.ren Flensted Lassen , Markus Sakari Kauppinen , Jack Bech Nielsen
发明人: Martin Schulein , Karen Margrethe Oxenb.o slashed.ll , Lene Nonboe Andersen , S.o slashed.ren Flensted Lassen , Markus Sakari Kauppinen , Jack Bech Nielsen
IPC分类号: C12N15/09 , C11D3/386 , C11D7/42 , C11D11/00 , C12N9/42 , C12N15/00 , C12N15/55 , C12N15/81 , C12R1/645 , C12R1/69 , C12R1/75 , C12S3/08 , C12S11/00 , D06M16/00 , D06P5/02 , D06P5/15 , D21C5/00 , D21C9/10 , D21H17/00 , D21H21/10 , C12S9/00
CPC分类号: D21H17/005 , C11D3/38645 , C12N15/81 , C12N9/2437 , C12Y302/01004 , D06M16/003 , D06P5/158 , D21C5/005 , D21H21/10
摘要: The present invention relates to an isolated enzyme exhibiting cellulolytic (endoglucanase) activity at alkaline pH, an enzyme composition comprising the enzyme, a DNA construct encoding the enzyme, methods for producing the enzyme or enzyme composition, a detergent composition comprising the enzyme, and methods for using the enzyme in, e.g., providing localized variation in the color density of dyed fabric, improving the drainage of an aqueous suspension of paper pulp, and de-inking of recycled paper.
摘要翻译: 本发明涉及在碱性pH下显示纤维素分解(内切葡聚糖酶)活性的分离酶,包含该酶的酶组合物,编码该酶的DNA构建体,用于制备该酶或酶组合物的方法,包含该酶的洗涤剂组合物和方法 用于使用酶,例如提供染色织物的颜色密度的局部变化,改善纸浆的水性悬浮液的排出和再循环纸的脱墨。
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公开(公告)号:US5919691A
公开(公告)日:1999-07-06
申请号:US809763
申请日:1997-03-20
申请人: Martin Schulein , Karen Margrethe Oxenb.o slashed.ll , Lene Nonboe Andersen , S.o slashed.ren Flensted Lassen , Markus Sakari Kauppinen , Jack Bech Nielsen
发明人: Martin Schulein , Karen Margrethe Oxenb.o slashed.ll , Lene Nonboe Andersen , S.o slashed.ren Flensted Lassen , Markus Sakari Kauppinen , Jack Bech Nielsen
IPC分类号: C12N15/09 , C11D3/386 , C11D7/42 , C11D11/00 , C12N9/42 , C12N15/00 , C12N15/55 , C12N15/81 , C12R1/645 , C12R1/69 , C12R1/75 , C12S3/08 , C12S11/00 , D06M16/00 , D06P5/02 , D06P5/15 , D21C5/00 , D21C9/10 , D21H17/00 , D21H21/10
CPC分类号: D21H17/005 , C11D3/38645 , C12N15/81 , C12N9/2437 , C12Y302/01004 , D06M16/003 , D06P5/158 , D21C5/005 , D21H21/10
摘要: DNA sequences are disclosed encoding an enzyme exhibiting endoglucanase activity. The endoglucanase is useful in a variety of industrial processes requiring an alkaline cellulase.
摘要翻译: PCT No.PCT / DK95 / 00400 Sec。 371日期1997年3月26日 102(e)1997年3月26日PCT提交1995年10月6日PCT公布。 公开号WO96 / 11262 日期1996年4月18日公开了编码显示内切葡聚糖酶活性的酶的DNA序列。 内切葡聚糖酶可用于需要碱性纤维素酶的各种工业过程中。
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