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40 条记录 (耗时 0.037 秒)

    DETECTION OF GLYCOPEPTIDES AND GLYCOPROTEINS FOR MEDICAL DIAGNOSTICS
    32.
    发明申请
    DETECTION OF GLYCOPEPTIDES AND GLYCOPROTEINS FOR MEDICAL DIAGNOSTICS 有权
    糖皮质激素检测及糖皮质激素用于医学诊断

    公开(公告)号：US20090053828A1

    公开(公告)日：2009-02-26

    申请号：US12164676

    申请日：2008-06-30

    申请人： Fred E. Regnier ,  Stephen B. Hooser ,  Christina R. Wilson ,  Wonryeon Cho

    发明人： Fred E. Regnier ,  Stephen B. Hooser ,  Christina R. Wilson ,  Wonryeon Cho

    IPC分类号： G01N33/68 ,  G01N33/53 ,  C07K4/00 ,  C07K14/435 ,  C07K9/00

    CPC分类号： G01N33/5091 ,  G01N33/6842 ,  G01N2400/10

    摘要： A diagnostic method for determining the absence or presence of a disease is provided. The method includes assaying the amount and/or types of glycopeptides in a sample from a subject, and comparing these to the amount and types of reference glycopeptides. The method may include the use of a stable isotope label, affinity selection, immunoaffinity chromatography, and glycoproteomics techniques, to identify and quantify changes in glycosylated peptides or glycosylated proteins associated with cancers such as malignant lymphoma or breast cancer, to monitor patient's response to therapy, and to monitor disease recurrence.

    摘要翻译： 提供了用于确定疾病的不存在或存在的诊断方法。 该方法包括测定来自受试者的样品中糖肽的量和/或类型，并将其与参考糖肽的量和类型进行比较。 该方法可以包括使用稳定的同位素标记，亲和力选择，免疫亲和层析和糖蛋白组学技术来鉴定和量化与癌症如恶性淋巴瘤或乳腺癌相关的糖基化肽或糖基化蛋白质的变化，以监测患者对治疗的反应 ，并监测疾病复发。

    Affinity selected signature peptides for protein identification and quantification
    33.
    发明申请
    Affinity selected signature peptides for protein identification and quantification 审中-公开
    亲和选择标签肽用于蛋白质鉴定和定量

    公开(公告)号：US20080145863A1

    公开(公告)日：2008-06-19

    申请号：US11786748

    申请日：2007-04-12

    申请人： Fred E. Regnier

    发明人： Fred E. Regnier

    IPC分类号： G01N33/53 ,  G01N33/00

    CPC分类号： G01N33/6848 ,  G01N33/6803 ,  G01N33/6842 ,  G01N33/6851 ,  Y10T436/24 ,  Y10T436/25

    摘要： A method for protein identification in complex mixtures that utilizes affinity selection of constituent proteolytic peptide fragments unique to a protein analyte. These “signature peptides” function as analytical surrogates. Mass spectrometric analysis of the proteolyzed mixture permits identification of a protein in a complex sample without purifying the protein or obtaining its composite peptide signature.

    摘要翻译： 一种利用蛋白质分析物特有的成分蛋白水解肽片段的亲和选择的复杂混合物中的蛋白质鉴定方法。 这些“签名肽”作为分析代理。 蛋白水解混合物的质谱分析允许在复杂样品中鉴定蛋白质而不纯化蛋白质或获得其复合肽标记。

    Detection of glycopeptides and glycoproteins for medical diagnostics
    35.
    发明授权
    Detection of glycopeptides and glycoproteins for medical diagnostics 有权
    检测用于医学诊断的糖肽和糖蛋白

    公开(公告)号：US08568993B2

    公开(公告)日：2013-10-29

    申请号：US12164676

    申请日：2008-06-30

    申请人： Fred E. Regnier ,  Stephen B. Hooser ,  Christina R. Wilson ,  Wonryeon Cho

    发明人： Fred E. Regnier ,  Stephen B. Hooser ,  Christina R. Wilson ,  Wonryeon Cho

    IPC分类号： G01N31/00 ,  G01N33/53

    CPC分类号： G01N33/5091 ,  G01N33/6842 ,  G01N2400/10

    摘要： A diagnostic method for determining the absence or presence of a disease is provided. The method includes assaying the amount and/or types of glycopeptides in a sample from a subject, and comparing these to the amount and types of reference glycopeptides. The method may include the use of a stable isotope label, affinity selection, immunoaffinity chromatography, and glycoproteomics techniques, to identify and quantify changes in glycosylated peptides or glycosylated proteins associated with cancers such as malignant lymphoma or breast cancer, to monitor patient's response to therapy, and to monitor disease recurrence.

    摘要翻译： 提供了用于确定疾病的不存在或存在的诊断方法。 该方法包括测定来自受试者的样品中糖肽的量和/或类型，并将其与参考糖肽的量和类型进行比较。 该方法可以包括使用稳定的同位素标记，亲和力选择，免疫亲和层析和糖蛋白组学技术来鉴定和量化与癌症如恶性淋巴瘤或乳腺癌相关的糖基化肽或糖基化蛋白质的变化，以监测患者对治疗的反应 ，并监测疾病复发。

    Differentially encoded biological analyzer planar array apparatus and methods
    36.
    发明授权
    Differentially encoded biological analyzer planar array apparatus and methods 有权
    差分编码生物分析仪平面阵列设备及方法

    公开(公告)号：US08298831B2

    公开(公告)日：2012-10-30

    申请号：US12466943

    申请日：2009-05-15

    申请人： David D. Nolte ,  Manoj Varma ,  Fred E. Regnier ,  Leilei Peng ,  Ming Zhao

    发明人： David D. Nolte ,  Manoj Varma ,  Fred E. Regnier ,  Leilei Peng ,  Ming Zhao

    IPC分类号： G01N21/76

    CPC分类号： G01N33/54373 ,  G01N21/45 ,  G01N21/553 ,  G01N21/645

    摘要： A method of probing a plurality of analyzer molecules distributed about a detection platform is disclosed. The method includes contacting a test sample to the plurality of analyzer molecules, scanning the plurality of analyzer molecules at a rate relating to a carrier frequency signal, and detecting the presence or absence of a biological molecule based at least in part upon the presence or absence of a signal substantially at a sideband of the carrier frequency signal. A molecule detection platform including a substrate and a plurality of targets positioned about the substrate is also disclosed. Specific analyzer molecules adapted to bind a specific analyte are immobilized about a first set of the targets. Nonspecific analyzer molecules are immobilized about a second set of the targets. The targets positioned about the substrate along at least a segment of a scanning pathway alternate between at least one of the first set and at least one of the second set. A method including providing a substrate for supporting biological analyzer molecules the substrate including at least one scanning pathway is also disclosed. The scanning pathway includes a plurality of scanning targets. Specific biological analyzer molecules adapted to detect a specific target analyte are distributed about a first set of the targets which alternate in groups of at least one with a second set of the targets the second set of the targets not including the specific biological analyzer molecules.

    摘要翻译： 公开了一种探测分布在检测平台周围的多个分析器分子的方法。 该方法包括使测试样本与多个分析器分子接触，以与载波频率信号相关的速率扫描多个分析器分子，并且至少部分地基于存在或不存在检测生物分子的存在或不存在 基本上在载波频率信号的边带处的信号。 还公开了一种分子检测平台，其包括基板和围绕基板定位的多个靶。 适于结合特定分析物的特异性分析仪分子被固定在第一组靶上。 非特异性分析仪分子被固定在第二组靶上。 沿着扫描通道的至少一段定位在基底周围的靶在第一组和第二组中的至少一个之间交替。 还公开了一种包括提供用于支持生物分析器分子的基板的方法，所述基板包括至少一个扫描路径。 扫描路径包括多个扫描目标。 适于检测特定目标分析物的特异性生物分析仪分子围绕第一组靶标分布，其中至少一组与第二组靶标交替，第二组靶标不包括特定生物分析仪分子。

    GROUP SPECIFIC INTERNAL STANDARD TECHNOLOGY (GSIST) FOR SIMULTANEOUS IDENTIFICATION AND QUANTIFICATION OF SMALL MOLECULES
    37.
    发明申请
    GROUP SPECIFIC INTERNAL STANDARD TECHNOLOGY (GSIST) FOR SIMULTANEOUS IDENTIFICATION AND QUANTIFICATION OF SMALL MOLECULES 有权
    集团特殊内部标准技术（GSIST）用于同时识别和定量小分子

    公开(公告)号：US20110183430A1

    公开(公告)日：2011-07-28

    申请号：US12990560

    申请日：2009-05-01

    申请人： Jiri Adamec ,  Wen-Chu Yang ,  Fred E. Regnier

    发明人： Jiri Adamec ,  Wen-Chu Yang ,  Fred E. Regnier

    IPC分类号： G01N24/00

    CPC分类号： G01N33/6842 ,  G01N33/6845

    摘要： Reagents and methods are provided that permit simultaneous analysis of multiple diverse small molecule analytes present in a complex mixture. Samples are labeled with chemically identical but isotopically distinct forms of the labeling reagent, and analyzed using mass spectrometry. A single reagent simultaneously derivatizes multiple small molecule analytes having different reactive functional groups.

    摘要翻译： 提供了允许同时分析复杂混合物中存在的多种不同小分子分析物的试剂和方法。 样品用化学相同但同位素不同的标记试剂标记，并用质谱分析。 单个试剂同时衍生具有不同反应性官能团的多个小分子分析物。

    Immobilized antigen-antibody displacement process
    40.
    发明授权
    Immobilized antigen-antibody displacement process 失效
    固定抗原 - 抗体置换过程

    公开(公告)号：US4895809A

    公开(公告)日：1990-01-23

    申请号：US569361

    申请日：1984-01-09

    申请人： Timothy D. Schlabach ,  Fred E. Regnier

    发明人： Timothy D. Schlabach ,  Fred E. Regnier

    IPC分类号： G01N30/74 ,  G01N30/84 ,  G01N33/53 ,  G01N33/538 ,  G01N33/543

    CPC分类号： G01N30/84 ,  G01N33/5302 ,  G01N33/538 ,  G01N33/54366 ,  G01N2021/6439 ,  G01N2030/746 ,  G01N30/74 ,  Y10S436/80 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/824 ,  Y10T436/25375 ,  Y10T436/255

    摘要： A method and apparatus for quantitatively analyzing, in a flowing stream, materials in a biological or chemical sample utilizes immobilized antobodies reversibly precharged with fluorescently labeled antigens. The labeled antigens are competitively displaced by unlabeled antigens in the sample, after the sample antigens have been segregated into zones by a chromatographic device, such as the reverse phase high performance liquid chromatograph. Displaced labeled antigens are measured by a fluorescence detector. A plurality of antibody species may be concurrently utilized, thereby allowing quantification of a plurality of antigens from a single sample.

    摘要翻译： 用于在流动流中定量分析生物或化学样品中的材料的方法和装置利用可逆地预先带有荧光标记抗原的固定化的本体。 在样品抗原已经通过色谱装置如反相高效液相色谱仪分离成区域后，标记的抗原在样品中被未标记的抗原竞争性置换。 通过荧光检测器测量位移标记的抗原。 可以同时使用多种抗体物种，从而允许从单个样品定量多种抗原。