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52 条记录 (耗时 0.028 秒)

    Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
    31.
    发明授权
    Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof 失效
    带有可淬灭荧光标记的寡核苷酸探针及其使用方法

    公开(公告)号：US06465175B2

    公开(公告)日：2002-10-15

    申请号：US09146157

    申请日：1998-09-03

    申请人： Thomas Horn ,  Hartmut R. Schroeder ,  Brian D. Warner ,  Ellen Fiss ,  Todd Sells ,  Say-Jong Law

    发明人： Thomas Horn ,  Hartmut R. Schroeder ,  Brian D. Warner ,  Ellen Fiss ,  Todd Sells ,  Say-Jong Law

    IPC分类号： C12Q168

    CPC分类号： C07H21/00 ,  C12Q1/6816 ,  C12Q1/6818

    摘要： Methods are provided for reducing background signals encountered in nucleic acid hybridization assays and other assays that involve hybridization of a labeled oligomer to its complement. The method is premised on the significant reduction of signal generation that occurs when a quenchable dye-labeled oligomer forms a hybrid complex. In addition, a method is provided for enhancing the detectable signal emitted from an amplification multimer hybridized to an oligomer probe to which a quenchable dye has been conjugated through a linker such that the emission from the dye is not quenched upon hybrid complex formation. Novel oligonucleotide probes are also provided that comprise an oligomer to which has been directly or indirectly through a linker a quenchable dye.

    摘要翻译： 提供了用于减少在核酸杂交测定中遇到的背景信号的方法和涉及标记的寡聚物与其互补体杂交的其它测定。 该方法的前提是当可淬灭的染料标记的寡聚物形成杂交复合物时发生的信号产生的显着减少。 此外，提供了一种用于增强从与已经通过接头共轭可淬灭染料的寡聚体探针杂交的扩增多聚体发射的可检测信号的方法，使得来自染料的发射在杂交复合物形成时不被淬灭。 还提供了新的寡核苷酸探针，其包含已经通过接头直接或间接通过可淬灭染料的低聚物。

    Oligonucleotides containing hydroxyl-protecting groups orthogonally removable by reduction
    32.
    发明授权
    Oligonucleotides containing hydroxyl-protecting groups orthogonally removable by reduction 失效
    含有通过还原正交除去的羟基保护基的寡核苷酸

    公开(公告)号：US5703218A

    公开(公告)日：1997-12-30

    申请号：US421798

    申请日：1995-04-13

    申请人： Michael S. Urdea ,  Thomas Horn

    发明人： Michael S. Urdea ,  Thomas Horn

    IPC分类号： C07H19/073 ,  C07H19/10 ,  C07H21/00 ,  C07H21/04

    CPC分类号： C07H19/10 ,  C07H21/00 ,  Y02P20/55

    摘要： Hydroxyl-protecting groups orthogonally removable by reduction with a liquid reducing agent are disclosed. The novel hydroxyl-protecting groups are particularly useful in the chemical synthesis of linear and branched oligonucleotide structures, as they are readily removed from the protected molecule with mild reagents such as dithionite. Examples of such hydroxyl-protecting groups include the 2-methylene-9,10-anthraquinone (Maq) carbonate ester and the p-nitrobenzyl carbonate ester.

    摘要翻译： 公开了通过用液体还原剂还原可正交除去的羟基保护基团。 新的羟基保护基团特别可用于直链和支链寡核苷酸结构的化学合成，因为它们用温和的试剂例如连二亚硫酸盐容易地从被保护的分子中除去。 这种羟基保护基的实例包括2-亚甲基-9,10-蒽醌（Maq）碳酸酯和对硝基苄基碳酸酯。

    Hydrophobic nucleic acid probe
    33.
    发明授权
    Hydrophobic nucleic acid probe 失效
    疏水核酸探针

    公开(公告)号：US5702893A

    公开(公告)日：1997-12-30

    申请号：US441192

    申请日：1995-05-15

    申请人： Michael S. Urdea ,  Thomas Horn

    发明人： Michael S. Urdea ,  Thomas Horn

    IPC分类号： C07H21/00 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C07H21/00 ,  C12Q1/6834

    摘要： A method for binding a target polynucleotide in a sample is provided. The method uses an assay reagent which is a substrate noncovalently bound to a hydrophobic moiety which is part of a polynucleotide capture probe. This assay reagent is contacted with the sample under conditions which permit hybridization between said capture probe and said target polynucleotide, thereby binding the target polynucleotide.

    摘要翻译： 提供了用于结合样品中的靶多核苷酸的方法。 该方法使用测定试剂，其是非共价结合到作为多核苷酸捕获探针的一部分的疏水部分的底物。 在允许所述捕获探针和所述靶多核苷酸之间杂交的条件下，使该测定试剂与样品接触，从而结合靶多核苷酸。

    Photolabile reagents for incorporation into oligonucleotide chains
    36.
    发明授权
    Photolabile reagents for incorporation into oligonucleotide chains 失效
    用于掺入寡核苷酸链的光不稳定试剂

    公开(公告)号：US5258506A

    公开(公告)日：1993-11-02

    申请号：US398711

    申请日：1989-08-25

    申请人： Michael S. Urdea ,  Thomas Horn

    发明人： Michael S. Urdea ,  Thomas Horn

    IPC分类号： C07H21/00 ,  C12Q1/68 ,  C07H21/02

    CPC分类号： C07H21/00 ,  C12Q1/6813

    摘要： Novel photolabile photochemical reagents are disclosed. The reagents are useful in a variety of biochemical and chemical contexts, including nucleic hybridization assays and chemical phosphorylation of hydroxyl-containing compounds. The reagents are particularly useful for introducing cleavable sites into oligonucleotide or polynucleotide chains, i.e., sites which are cleavable upon photolysis. The reagents are also useful in both 5'- and 3'-phosphorylation of oligonucleotide or polynucleotide chains.

    摘要翻译： 公开了新型光不稳定光化学试剂。 这些试剂可用于各种生化和化学环境，包括核酸杂交测定和含羟基化合物的化学磷酸化。 这些试剂特别可用于将可切割位点引入寡核苷酸或多核苷酸链，即在光解后可切割的位点。 这些试剂也可用于寡核苷酸或多核苷酸链的5'-和3'-磷酸化。

    Method for making nucleic acid probes
    37.
    发明授权
    Method for making nucleic acid probes 失效
    制作核酸探针的方法

    公开(公告)号：US4910300A

    公开(公告)日：1990-03-20

    申请号：US190698

    申请日：1988-05-05

    申请人： Michael S. Urdea ,  Thomas Horn

    发明人： Michael S. Urdea ,  Thomas Horn

    IPC分类号： C07H21/00 ,  C12Q1/68 ,  C12Q1/70

    CPC分类号： C07H21/00 ,  C12Q1/6813 ,  C12Q1/682 ,  C12Q1/706 ,  Y02P20/55

    摘要： Modified nucleotides are provided which have the structure ##STR1## wherein R.sup.1 is a reactive group derivatizabale with a detectable label, R.sup.2 is an optional linking moiety including an amide, thioether or disulfide linkage or a combination thereof. R.sup.3 is hydrogen, methyl, bromine, fluorine or iodine, R.sup.4 is hydrogen, an acid-sensitive, base-stable blocking group or an acyl capping group, R.sup.5 is hydrogen or a phosphorus derivative, R.sup.6 is H, OH, or OR where R is a protecting group and x is an integer in the range of 1 and 8 inclusive. Methods of synthesizing the derivatizable nucleotide are disclosed, as are labeled polynucleotide probes prepared therefrom.

    摘要翻译： 提供了具有结构“IMAGE”的修饰的核苷酸，其中R 1是具有可检测标记的反应性基团衍生化物，R 2是任选的连接部分，包括酰胺，硫醚或二硫键或其组合。 R3是氢，甲基，溴，氟或碘，R4是氢，酸敏感的碱稳定的封闭基团或酰基封端基团，R 5是氢或磷衍生物，R 6是H，OH或OR，其中R 是保护基，x是1和8的整数。 公开了合成可衍生核苷酸的方法，以及由其制备的标记的多核苷酸探针。

    Human papilloma virus peptide-specific T-cell response for treatment of warts
    38.
    发明申请
    Human papilloma virus peptide-specific T-cell response for treatment of warts 审中-公开
    人乳头状瘤病毒肽特异性T细胞治疗疣的反应

    公开(公告)号：US20120045413A1

    公开(公告)日：2012-02-23

    申请号：US13136801

    申请日：2011-08-11

    申请人： Mayumi Nakagawa ,  Kevin Kim ,  Thomas Horn

    发明人： Mayumi Nakagawa ,  Kevin Kim ,  Thomas Horn

    IPC分类号： A61K39/12 ,  C07K7/08 ,  C07K7/06 ,  A61P31/20 ,  A61K38/19 ,  A61K38/21 ,  A61K38/20 ,  A61P35/00 ,  C07K14/025 ,  A61K39/165

    CPC分类号： C07K14/40 ,  A61K38/16 ,  A61K39/0002 ,  A61K39/12 ,  A61K2039/54 ,  A61K2039/58 ,  A61K2039/585 ,  C12N2710/20011

    摘要： The inventors have treated human patients for warts by intralesional injection of Candida antigen to induce a delayed-type hypersensitivity response in the patients. This creates an immune response that recognizes the antigens of the human papilloma virus (HPV) found in and causing the warts. It was found that the patients showed a response to HPV type 57 L1 peptide 380-412 (a peptide consisting of amino acid residues 380-412 of the protein L1) and HPV type 57 protein E4 (E4 10-30). One embodiment of the invention provides a pharmaceutical composition comprising a polypeptide comprising (a) L1 380-412 (SEQ ID NO:3) or a fragment of at least 8 residues of SEQ ID NO:3 or (b) E4 10-30 (SEQ ID NO:4) or a fragment of at least 8 residues of E4 10-30 (SEQ ID NO:4), wherein the composition is immunogenic in humans.

    摘要翻译： 本发明人通过病灶内注射假丝酵母抗原来治疗疣，以诱发患者的迟发型超敏反应。 这产生了识别发现于和导致疣的人乳头状瘤病毒（HPV）的抗原的免疫应答。 发现患者对HPV 57 L1肽380-412（由蛋白L1的氨基酸残基380-412构成的肽）和HPV 57型蛋白E4（E4 10-30）显示出反应。 本发明的一个实施方案提供了包含多肽的药物组合物，其包含（a）L1 380-412（SEQ ID NO：3）或至少8个残基的SEQ ID NO：3或（b）E4 10-30（ SEQ ID NO：4）或E4 10-30（SEQ ID NO：4）的至少8个残基的片段，其中所述组合物在人体中是免疫原性的。

    Arrangement for monitoring patch fields at distribution points in data networks using reader coils to detect and communicate with transponders
    39.
    发明授权
    Arrangement for monitoring patch fields at distribution points in data networks using reader coils to detect and communicate with transponders 失效
    用于使用读取器线圈检测和与应答器通信的数据网络中的分发点监视补丁字段的布置

    公开(公告)号：US07629893B2

    公开(公告)日：2009-12-08

    申请号：US11618602

    申请日：2006-12-29

    申请人： Thomas Horn

    发明人： Thomas Horn

    IPC分类号： G08B21/00

    CPC分类号： G01R31/045 ,  H04L43/0817

    摘要： An arrangement for monitoring patch fields at distribution points in data networks comprises patch cables which can be plugged by means of plugs into connectors in the patch fields. Each of the two plugs of each patch cable is provided with a transponder. A device is arranged before or in the proximity of one of the patch fields with its connectors. The device is equipped with readers and reader coils. The readers are in data communication with an evaluation unit. The device comprises a plurality of reader coils, the number of which exceeds the number of connectors in the patch field. The reader coils can be independently driven.

    摘要翻译： 用于在数据网络中的分发点处监视补丁字段的布置包括可以通过插头插入到补丁字段中的连接器中的跳线电缆。 每个跳线电缆的两个插头中的每一个都设置有应答器。 一个设备布置在其中一个补片区域之前或附近，并具有其连接器。 该设备配有读卡器和读卡器线圈。 读者与评估单元进行数据通信。 该装置包括多个读取器线圈，其数量超过了补片区域中的连接器的数量。 读取器线圈可以独立驱动。