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    Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
    1.
    发明授权
    Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof 失效
    带有可淬灭荧光标记的寡核苷酸探针及其使用方法

    公开(公告)号：US06465175B2

    公开(公告)日：2002-10-15

    申请号：US09146157

    申请日：1998-09-03

    Applicant: Thomas Horn ,  Hartmut R. Schroeder ,  Brian D. Warner ,  Ellen Fiss ,  Todd Sells ,  Say-Jong Law

    Inventor： Thomas Horn ,  Hartmut R. Schroeder ,  Brian D. Warner ,  Ellen Fiss ,  Todd Sells ,  Say-Jong Law

    IPC: C12Q168

    CPC classification number: C07H21/00 ,  C12Q1/6816 ,  C12Q1/6818

    Abstract: Methods are provided for reducing background signals encountered in nucleic acid hybridization assays and other assays that involve hybridization of a labeled oligomer to its complement. The method is premised on the significant reduction of signal generation that occurs when a quenchable dye-labeled oligomer forms a hybrid complex. In addition, a method is provided for enhancing the detectable signal emitted from an amplification multimer hybridized to an oligomer probe to which a quenchable dye has been conjugated through a linker such that the emission from the dye is not quenched upon hybrid complex formation. Novel oligonucleotide probes are also provided that comprise an oligomer to which has been directly or indirectly through a linker a quenchable dye.

    Abstract translation: 提供了用于减少在核酸杂交测定中遇到的背景信号的方法和涉及标记的寡聚物与其互补体杂交的其它测定。 该方法的前提是当可淬灭的染料标记的寡聚物形成杂交复合物时发生的信号产生的显着减少。 此外，提供了一种用于增强从与已经通过接头共轭可淬灭染料的寡聚体探针杂交的扩增多聚体发射的可检测信号的方法，使得来自染料的发射在杂交复合物形成时不被淬灭。 还提供了新的寡核苷酸探针，其包含已经通过接头直接或间接通过可淬灭染料的低聚物。

    Planar Waveguide Detection Chips and Chambers for Performing Multiple PCR Assays
    3.
    发明申请
    Planar Waveguide Detection Chips and Chambers for Performing Multiple PCR Assays 审中-公开
    用于执行多个PCR测定的平面波导检测芯片和室

    公开(公告)号：US20090075273A1

    公开(公告)日：2009-03-19

    申请号：US12092280

    申请日：2006-11-09

    Applicant: Ingmar Dorn ,  John J. Quinn ,  Brian D. Warner

    Inventor： Ingmar Dorn ,  John J. Quinn ,  Brian D. Warner

    IPC: C12Q1/68 ,  C12M1/34

    CPC classification number: G01N21/6428 ,  G01N21/648 ,  G01N21/7703

    Abstract: Provided are planar waveguide (“PWG”) detection chips that are used to perform multiplex PCR and kinetic PCR assays with a single fluorescent dye. The PWG detection chips are housed in PWG detection chambers that house at least one PWG chip. The PWG detection chambers may be in a single chamber or a dual chamber configuration. Also provided are methods for analyzing amplification products using the PWG detection chambers of the present invention.

    Abstract translation: 提供了用于使用单个荧光染料进行多重PCR和动力学PCR测定的平面波导（“PWG”）检测芯片。 PWG检测芯片安装在容纳至少一个PWG芯片的PWG检测室中。 PWG检测室可以在单室或双室配置中。 还提供了使用本发明的PWG检测室分析扩增产物的方法。

    Luminometer with reduced sample crosstalk
    7.
    发明授权
    Luminometer with reduced sample crosstalk 失效
    发光计具有减少的样品串扰

    公开(公告)号：US5401465A

    公开(公告)日：1995-03-28

    申请号：US878829

    申请日：1992-05-05

    Applicant: Rick T. Smethers ,  Brian D. Warner ,  Victor P. Burolla

    Inventor： Rick T. Smethers ,  Brian D. Warner ,  Victor P. Burolla

    IPC: G01N21/64 ,  G01N21/03 ,  G01N21/76 ,  G01N21/78 ,  G01N33/532 ,  G01N33/536 ,  G01N33/543 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04

    CPC classification number: G01N21/76 ,  G01N35/028 ,  G01N2035/00376 ,  G01N2035/0487 ,  Y10S435/808

    Abstract: A luminometer comprising a tray for receiving an array of sample wells, a photodetector assembly, and device for relatively moving the tray and photodetector assembly in one direction to align the sample wells received by the tray in a predetermined sequence with the photodetector assembly. The photodetector assembly of the luminometer includes a stage, a photodetection head having a detection aperture permitting passage of light therethrough, a device for mounting the photodetection head to the stage that permits movement of the head in a direction substantially normal to the direction of relative tray and photodetector assembly movement, and a device for biasing the photodetection head toward a selected sample well so that the detection aperture is substantially isolated from light emitted from adjacent wells. Internal and external photodetector calibration systems and a sample heater are also contemplated.

    Abstract translation: 一种发光计，包括用于接收样品阱阵列的光盘，光电检测器组件和用于使托盘和光电检测器组件在一个方向上相对移动的装置，以使由托盘接收的样品阱以预定顺序与光电检测器组件对准。 该发光计的光电检测器组件包括一台，具有允许光通过的检测孔的光电检测头，用于将光电检测头安装到台架上的装置，该装置允许头沿基本上垂直于相对托盘方向移动 和光电检测器组件移动，以及用于将光电检测头朝向所选样本阱偏置的装置，使得检测孔与从相邻孔发射的光基本上隔离。 还考虑了内部和外部光电探测器校准系统和样品加热器。

    Dual-purpose primers and probes for providing enhanced hybridization assays by disruption of secondary structure formation
    9.
    发明授权
    Dual-purpose primers and probes for providing enhanced hybridization assays by disruption of secondary structure formation 有权
    双用途引物和探针，通过破坏二级结构形成提供增强的杂交测定

    公开(公告)号：US08394944B2

    公开(公告)日：2013-03-12

    申请号：US10667191

    申请日：2003-09-15

    Applicant: Minxue Zheng ,  John J. Quinn ,  Brian D. Warner

    Inventor： Minxue Zheng ,  John J. Quinn ,  Brian D. Warner

    IPC: C07H21/04 ,  C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/6858 ,  C12Q1/6827 ,  C12Q1/6832 ,  C12Q1/686 ,  C12Q2565/519 ,  C12Q2525/301 ,  C12Q2525/197 ,  C12Q2525/186 ,  C12Q2525/313

    Abstract: The present invention provides primers and probes to be used in a method of enhancing hybridization of a probe to a target nucleotide sequence when the target sequence is capable of forming intramolecular secondary structures that interfere with hybridization of the probe to the target sequence. In particular, the invention includes a primer for amplifying a target nucleotide sequence, wherein at least a portion of the target nucleotide sequence can form an intramolecular secondary structure. The primer of the invention includes a primer nucleotide sequence complementary to a portion of the target nucleotide sequence that does not form a secondary structure, and a blocking sequence substantially complementary to at least a portion of the secondary structure-forming region of the amplified target nucleotide sequence, wherein the blocking sequence hybridizes to a portion of the secondary structure-forming region of the amplified target nucleotide sequence and blocks the formation of the secondary structure.

    Abstract translation: 本发明提供了当靶序列能够形成干扰探针与靶序列的杂交的分子内二级结构时，用于增强探针与靶核苷酸序列的杂交的方法中的引物和探针。 特别地，本发明包括用于扩增靶核苷酸序列的引物，其中目标核苷酸序列的至少一部分可以形成分子内二级结构。 本发明的引物包括与未形成二级结构的靶核苷酸序列的一部分互补的引物核苷酸序列和与扩增的靶核苷酸的至少一部分二级结构形成区基本上互补的阻断序列 序列，其中所述阻断序列与扩增的靶核苷酸序列的二级结构形成区的一部分杂交并阻断二级结构的形成。

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