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    Optimized sample injection structures in microfluidic separations
    31.
    发明授权
    Optimized sample injection structures in microfluidic separations 有权
    微流体分离中优化的样品注射结构

    公开(公告)号：US07749365B2

    公开(公告)日：2010-07-06

    申请号：US11460236

    申请日：2006-07-26

    Applicant: Michael Nguyen ,  Luc Bousse

    Inventor： Michael Nguyen ,  Luc Bousse

    IPC: G01N27/26 ,  B81B7/00

    CPC classification number: C07K1/26 ,  G01N27/44791 ,  Y10T436/2575

    Abstract: The invention herein provides improved sample injection systems and related methods to create microfluidic devices with symmetrical channel configurations that can produce relatively large sample volumes. An embodiment of the invention provides microfluidic structures with different geometries that are symmetrical from the perspective of a sample load channel and a sample waste channel, which essentially eliminates issues of time offset and other problems commonly associated with twin-T sample formation techniques. A split-injection approach and related methods of sample plug formation are therefore provided.

    Abstract translation: 本文的发明提供了改进的样品注射系统和相关方法来产生具有对称通道构型的微流体装置，其可以产生相对较大的样品体积。 本发明的一个实施方案提供了具有不同几何形状的微流体结构，其从样品负载通道和样品废物通道的角度对称，其基本上消除了时间偏移和通常与双T样品形成技术相关的其它问题的问题。 因此提供了分离注射方法和样品塞形成的相关方法。

    SERIAL ELECTROPHORESIS
    33.
    发明申请
    SERIAL ELECTROPHORESIS 有权

    公开(公告)号：US20250060332A1

    公开(公告)日：2025-02-20

    申请号：US18935935

    申请日：2024-11-04

    Applicant: IntegenX Inc.

    Inventor： Mattias Vangbo ,  David King ,  Arnaldo Barican

    IPC: G01N27/447 ,  C12Q1/6806 ,  C12Q1/686

    Abstract: A system for outputting electropherograms includes a capillary containing a separation medium and comprising an inlet end, a distal end, and an interrogation region between the inlet and distal ends, the inlet end of the capillary configured to receive a plurality of differing samples containing DNA fragments having a plurality of different sizes; a power source configured to selectively apply forward and reverse polarity voltages between the inlet end and the distal end of the capillary; a detector configured to detect signal associated with DNA fragments moving through the interrogation region of the capillary; and a processor communicatively coupled to the detector and configured to process signal detected by the detector and output electropherograms during an electrophoresis run, the electropherograms corresponding to a plurality of differing samples successively introduced to the capillary from the inlet and traveling through the capillary at the same time.

    Compositions, methods, kits and devices for molecular analysis
    35.
    发明授权
    Compositions, methods, kits and devices for molecular analysis 有权

    公开(公告)号：US12153014B2

    公开(公告)日：2024-11-26

    申请号：US18108471

    申请日：2023-02-10

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  INTEGENX INC.

    Inventor： Jen-Kuei Liu ,  Bharti Solanki ,  Hans Michael Wenz ,  Frank Mercer ,  Cevat Akin ,  Achim Karger ,  Annelise Barron ,  Scott Mack

    IPC: G01N27/447 ,  C08L33/26

    Abstract: Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C.
    Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.

    Serial electrophoresis
    36.
    发明授权
    Serial electrophoresis 有权

    公开(公告)号：US12135309B2

    公开(公告)日：2024-11-05

    申请号：US18157274

    申请日：2023-01-20

    Applicant: IntegenX, Inc.

    Inventor： Mattias Vangbo ,  David King ,  Arnaldo Barican

    IPC: G01N27/447 ,  C12Q1/6806 ,  C12Q1/686

    Abstract: A system for performing capillary electrophoresis of multiple samples comprises a capillary containing a separation medium and having inlet and distal ends and an interrogation region; a power source configured to apply voltages between inlet and distal ends; and logic to cause execution of: applying a first substantially constant forward polarity electrophoresis voltage to the capillary; before all of the first DNA fragments have passed the interrogation region, applying a reverse polarity voltage pulse to the capillary, thereby transporting at least some of the first DNA fragments in the capillary toward the capillary inlet; introducing a second sample to the capillary inlet, the second sample comprising second DNA fragments having a plurality of different sizes; and applying a second substantially constant forward polarity electrophoresis voltage to the capillary to simultaneously perform electrophoresis on the second DNA fragments and the first DNA fragments.

    CONTROLLING DNA CONCENTRATION FOR STR ANALYSIS
    37.
    发明申请
    CONTROLLING DNA CONCENTRATION FOR STR ANALYSIS 有权

    公开(公告)号：US20220016632A1

    公开(公告)日：2022-01-20

    申请号：US17311263

    申请日：2019-12-04

    Applicant: Integenx, Inc.

    Inventor： Mattias Vangbo ,  David King

    IPC: B01L3/00 ,  C12Q1/6858 ,  B01L7/00

    Abstract: Performing sample quantitation and sample amplification may be performed in a sample cartridge or sample cartridges. Sample quantitation using qPCR may be performed during STR PCR on the sample. Samples need not be normalized prior to performing STR PCR. In certain embodiments, qPCR and STR PCR are performed on the same cartridge, optionally at the same time (or in real-time, or overlapping in time) and optionally using some or all of the same PCR apparatus. In other embodiments, qPCR and STR PCR are performed on different cartridges. Quantitation of the STR PCR sample may be performed without substantially delaying the STR PCR process.

    Sample Preparation, Processing and Analysis Systems
    38.
    发明申请
    Sample Preparation, Processing and Analysis Systems 有权

    公开(公告)号：US20210277445A1

    公开(公告)日：2021-09-09

    申请号：US17119874

    申请日：2020-12-11

    Applicant: Integenx Inc.

    Inventor： David Eberhart ,  Yuan Li ,  James Ogg ,  Ezra Van Gelder ,  Stephen J. Williams ,  Timothy Woudenberg ,  Dean S. Burgi ,  William D. Nielsen

    IPC: C12Q1/686 ,  B01L3/00 ,  B01L7/00

    Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

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