公开(公告)号：US20090087882A1

公开(公告)日：2009-04-02

申请号：US12231168

申请日：2008-08-28

申请人： Roland Contreras ,  Steven Geysens

发明人： Roland Contreras ,  Steven Geysens

IPC分类号： C12P21/04 ,  C12N1/19

CPC分类号： C12Y302/01113 ,  C12N9/2434 ,  C12P21/00 ,  C12Y204/0115 ,  C12Y302/0102 ,  C12Y302/01084

摘要： The invention relates to the use of a glucosidase II mutation to increase protein secretion in yeast cells. The invention relates further to the use of yeast cells, comprising a mutant glucosidase II gene, possibly in combination with the expression of a recombinant α-1,2-mannosidase gene and/or a recombinant N-acetylglucosaminyl-transferase gene, as a host for protein secretion.

摘要翻译： 本发明涉及使用葡糖苷酶II突变增加酵母细胞中的蛋白质分泌。 本发明进一步涉及包含可能与重组α-1,2-甘露糖苷酶基因和/或重组N-乙酰葡糖胺基转移酶基因的表达组合的突变型葡糖苷酶II基因作为宿主的酵母细胞的使用 用于蛋白质分泌。

公开(公告)号：US20090069232A1

公开(公告)日：2009-03-12

申请号：US12062469

申请日：2008-04-03

申请人： Nico Luc Marc Callewaert ,  Wouter Vervecken ,  Karen Jacqueline Marcel De Pourcq ,  Steven Christian Jozef Geysens ,  Mouna Guerfal

发明人： Nico Luc Marc Callewaert ,  Wouter Vervecken ,  Karen Jacqueline Marcel De Pourcq ,  Steven Christian Jozef Geysens ,  Mouna Guerfal

IPC分类号： A61K38/00 ,  C12P21/06 ,  C07K14/00 ,  C12N15/63 ,  C07H21/04 ,  C12N1/19

CPC分类号： C12N9/2488 ,  A61K38/00 ,  C07K14/00 ,  C12N9/1051 ,  C12N9/24 ,  C12N9/2402 ,  C12N9/2465 ,  C12P21/005 ,  C12P21/06 ,  C12Y204/01232 ,  C12Y302/01024 ,  C12Y302/01045 ,  C12Y302/01046 ,  C12Y302/01084 ,  C12Y302/01113 ,  Y02P20/52

摘要： Described herein are methods and genetically engineered cells useful for producing an altered N-glycosylation form of a target molecule. Also described are methods and molecules with altered N-glycosylation useful for treating a variety of disorders such as metabolic disorders.

摘要翻译： 本文描述了可用于产生改变的N-糖基化形式的靶分子的方法和遗传工程改造的细胞。 还描述了具有改变的N-糖基化的方法和分子，其可用于治疗各种疾病如代谢紊乱。

公开(公告)号：US20020013953A1

公开(公告)日：2002-01-31

申请号：US09770496

申请日：2001-01-29

发明人： Arnold J. Reuser ,  Ans T. Van der Ploeg ,  Martin Ph. Verbeet

IPC分类号： A01K067/027

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2227/107 ,  A01K2267/01 ,  A01K2267/03 ,  A23C9/20 ,  A23C2230/05 ,  A61K38/47 ,  C12N9/2408 ,  C12Y302/01084

摘要： The invention provides transgenic nonhuman mammals producing phosphorylated lysosomal proteins in their milk, methods of generating the same, pharmaceutical compositions for use in enzyme replacement therapy, and methods of treating Pompe's disease using human acid alpha glucosidase.

公开(公告)号：US5889179A

公开(公告)日：1999-03-30

申请号：US98368

申请日：1998-06-17

申请人： Gregory L. Cote ,  Herbert Wyckoff

发明人： Gregory L. Cote ,  Herbert Wyckoff

IPC分类号： C12N9/24 ,  C07H1/00

CPC分类号： C12Y302/01084 ,  C12N9/1051 ,  C12N9/2402

摘要： A new enzyme, alternanase, which is effective for the endo-hydrolytic cleavage of alternan, producing a thinned composition of low molecular weight fractions which exhibit reduced viscosity and increased solubility relative to native alternan, is described. The enzyme is produced and secreted extracellularly by a plurality of novel bacteria isolated from soil. One of the fractions present in the thinned alternan resulting from hydrolysis with alternanase is a the cyclic tetrasaccharide, cyclo{-6)-.alpha.-D-Glcp-(1,3)-.alpha.-D-Glcp-(1,6)-.alpha.-D-Glcp-(1,3)-.alpha.-D-Glcp-(1-}. A novel method for isolating strains of microorganisms which produce endo-.alpha.-D-glucanases such as alternanase effective for the endo-hydrolytic cleavage or thinning of alternan is also described. Cultures of the subject strains are contacted with a test substrate of alternan coupled to a detectable indicator. Detection of released indicator provides an indication of endo-.alpha.-D-glucanase activity.

公开(公告)号：US5306639A

公开(公告)日：1994-04-26

申请号：US772850

申请日：1991-10-08

申请人： Aizo Matsushiro

发明人： Aizo Matsushiro

IPC分类号： A61K8/99 ,  A61K38/00 ,  A61Q11/00 ,  C12N9/12 ,  C12N9/24 ,  C12N9/86 ,  C12N15/31 ,  C12N15/74

CPC分类号： A61Q11/00 ,  A61K39/092 ,  A61K8/99 ,  C12N15/743 ,  C12N9/12 ,  C12N9/2408 ,  C12N9/86 ,  C12Y302/01011 ,  C12Y302/01059 ,  C12Y302/01071 ,  C12Y302/01084 ,  A61K38/00 ,  C07K2319/02

摘要： Disclosed is the isolation and cloning of genes encoding glucanohydrolase enzymes and the expression and secretion of exogenous glucanase gene products in host cells.

摘要翻译： 公开了编码葡聚糖水解酶的基因的分离和克隆以及外源葡聚糖酶基因产物在宿主细胞中的表达和分泌。

公开(公告)号：US4328313A

公开(公告)日：1982-05-04

申请号：US232595

申请日：1981-02-09

申请人： Lloyd G. Simonson ,  Burton L. Lamberts

发明人： Lloyd G. Simonson ,  Burton L. Lamberts

IPC分类号： A61K38/00 ,  C12N9/24

CPC分类号： C12Y302/01071 ,  C12N9/2402 ,  C12Y302/01084 ,  A61K38/00 ,  Y10S435/874

摘要： A method of producing .alpha.-1, 3-glucanase by introducing a bacterial culture such as Pseudomonas sp. isolate NRRL B-12324 into an aqueous medium containing a "limit glucan" substrate which is greater than 90 percent .alpha.-1, 3-glycosidically linked, then allowing growth to take place to accumulate .alpha.-1, 3-glucanase, and then recovering the enzyme for use as an oral therapeutic agent.

摘要翻译： 通过引入细菌培养物如假单胞菌属（Pseudomonas sp。）来产生α-1,3-葡聚糖酶的方法。 将NRRL B-12324分离成含有“极性葡聚糖”底物的水性培养基，其大于90％的α-1,3-糖苷连接，然后允许生长以积累α-1,3-葡聚糖酶，然后回收 用作口服治疗剂的酶。