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公开(公告)号:US20200299650A1
公开(公告)日:2020-09-24
申请号:US16088743
申请日:2017-03-31
发明人: Guang QU , Younghoon OH , Lin LU , John Fraser WRIGHT
摘要: In accordance with the invention, provided herein are methods for purifying recombinant adeno-associated (rAAV) vector particles.
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公开(公告)号:US20200248238A1
公开(公告)日:2020-08-06
申请号:US16637462
申请日:2018-08-08
IPC分类号: C12Q1/689 , C12N1/06 , C12Q1/6806 , C12Q1/686
摘要: The present invention provides a method for detecting a microorganism in a sample, the method comprising: a) filtering a sample through a filter to entrap any microorganisms present in the sample; b) treating the filter to release genomic material or DNA from the entrapped microorganisms; c) amplifying the genomic material or DNA released from the entrapped microorganisms; and d) identifying specific regions of the genomic material or DNA to determine the presence, identify the species or quantify the approximate number of any entrapped microorganisms.
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公开(公告)号:US10709776B2
公开(公告)日:2020-07-14
申请号:US15302279
申请日:2015-04-07
申请人: INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE) , CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) , INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA) , UNIVERSITÉ PAUL SABATIER TOULOUSE III , CENTRE HOSPITALIER UNIVERSITAIRE DE TOULOUSE
发明人: Eric Oswald , Patricia Martin , Kazunori Murase
IPC分类号: A61K39/108 , C12N1/20 , A61K39/00 , C12N1/06 , C07K14/245 , C12N1/02 , C12Q1/18
摘要: The present invention relates to ex vivo method for producing outer membrane vesicles (OMVs) by expression or overexpression of the hemolysin F gene (hlyF) in gram-negative bacterium.
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34.
公开(公告)号:US20200016594A1
公开(公告)日:2020-01-16
申请号:US16338306
申请日:2017-09-29
发明人: Aaron Ray WHEELER , Michael Dean CHAMBERLAIN , Julian Lucas LAMANNA , Michael David Murdoch DRYDEN , Elena KOLOMIETZ , David CHITAYAT
IPC分类号: B01L3/00 , C12N1/06 , C12Q1/6806 , G01N1/28 , G01N15/14
摘要: The present disclosure provides a system and method for identifying and targeting individual cells within a cell population for selective extraction of cellular content and a digital microfluidic device having at least one hydrophilic site for receiving cells, an imaging system including a stage for receiving the digital microfluidic device and an imaging module for identifying at least one targeted cell among the cells at the at least one hydrophilic site. The system includes a pulsed laser source for laser lysing the targeted cell thereby releasing the cell content to produce a lysate. A control system controls the pulsed laser source, the imaging system and the digital microfluidic device and is programmed for coordinating steps of i) movement of droplets on the digital microfluidic device, ii) selection of the at least one targeted cell to be lysed located at the at least one hydrophilic site, iii) illumination of the at least one selected targeted cell by the pulsed laser source to lyse the at least one selected targeted cell to produce lysate, and iv) collection of the lysate.
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公开(公告)号:US10527617B2
公开(公告)日:2020-01-07
申请号:US16176234
申请日:2018-10-31
发明人: Takamitsu Utsumi , Koji Maehana , Kenji Matsuyama
摘要: The object is to provide a lysis method and lysis treatment solution for efficiently lysing cells of various Streptococcus bacteria in milk of a livestock animal to release a specific antigen substance contained in the cells for detecting whether causative bacterium of mastitis is a Streptococcus bacterium or not by using the milk, as well as a detection method using an immunochromatographic device. There is provided a method for lysing a Streptococcus bacterium, which comprises the step of mixing a lysis agent containing a lytic enzyme with milk obtained form a livestock animal to lyse a Streptococcus bacterium existing in the milk. The lytic enzyme is preferably at least one selected from the group consisting of lysozyme, labiase, and β-N-acetylglucosaminidase.
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36.
公开(公告)号:US20190310214A1
公开(公告)日:2019-10-10
申请号:US16440829
申请日:2019-06-13
摘要: Various devices, systems and methods for detecting the presence of β-lactam antibiotic hydrolyzing bacteria in a sample are disclosed. In one embodiment, a method of detecting the presence of β-lactam antibiotic hydrolyzing bacteria includes introducing the sample to a filter configured to capture bacteria in the sample; introducing a buffer solution to the bacteria captured on the filter surface, wherein the buffer solution comprises a β-lactamase substrate, a β-lactamase activator, and a lysing agent to lyse cells of the bacteria to release β-lactam antibiotic hydrolyzing enzymes; exposing a sensor having an electrical characteristic to a reaction mixture comprising the buffer solution and the β-lactam antibiotic hydrolyzing enzymes; and monitoring a change in the electrical characteristic of the sensor to detect the presence of the β-lactam antibiotic hydrolyzing bacteria in the sample originally introduced.
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公开(公告)号:US10436783B2
公开(公告)日:2019-10-08
申请号:US15569143
申请日:2016-04-28
发明人: Yuji Saito , Daisuke Kato
IPC分类号: G01N33/53 , G01N33/569 , C12N1/00 , C12Q1/04 , C12N1/06 , G01N33/543 , C07K1/36 , C07K14/195
摘要: This invention provides a method for easily collecting antigens possessed by microorganisms without the use of special equipment. The method for collecting microbial antigens comprises: allowing a specimen containing microorganisms to pass through a filter membrane with a pore diameter that does not allow microorganisms to pass therethrough; capturing the microorganisms in the specimen on the filter membrane; applying a microbial destruction reagent capable of microbial membrane destruction to the filter membrane comprising the microorganisms captured thereon to destruct the captured microorganisms on the filter membrane; and collecting antigens in the filtrate.
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公开(公告)号:US10428301B2
公开(公告)日:2019-10-01
申请号:US15299059
申请日:2016-10-20
发明人: Robert Doebler , Ali Nadim , James D. Sterling , Anna Hickerson , Barbara Erwin , Denice Woyski , Ryan P. Talbot , Bruce Irvine
摘要: Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency.
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公开(公告)号:US20190256893A1
公开(公告)日:2019-08-22
申请号:US16399305
申请日:2019-04-30
IPC分类号: C12Q1/6806 , G01N33/569 , C12N1/06 , C12Q1/70
摘要: A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.
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公开(公告)号:US20190218595A1
公开(公告)日:2019-07-18
申请号:US16364936
申请日:2019-03-26
发明人: Bart Edward Gusta Jozef Van MEERBERGEN , Oana Mihaela PICIU , Ron GILL , Kristiane Anne SCHMIDT , Sieglinde NEERKEN , Marc Wilhelmus Gijsbert PONJEE , Zeynep Seflek Unay , Roel PENTERMAN , Paul Arnold VAN DE WIEL
IPC分类号: C12Q1/6806 , C12N1/06 , C12Q1/6895 , C12Q1/689
CPC分类号: C12Q1/6806 , C12N1/06 , C12Q1/689 , C12Q1/6895 , G01N33/48 , C12Q2527/125 , C12Q2527/119
摘要: Methods and devices for detection of micro-organisms present or suspected to be present within a mammalian blood sample are provided. A selective lysis is obtained by incubating the sample in a non-ionic detergent under alkaline conditions.
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