-
1.发明授权公开(公告)号：US10155819B2
公开(公告)日：2018-12-18
申请号：US14165896
申请日：2014-01-28
发明人： James M Cregg , John Latham , Mark Litton , Randal Schatzman , Ilya Tolstrorukov
摘要： Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.
-
2.发明申请公开(公告)号：US20160113920A1
公开(公告)日：2016-04-28
申请号：US14922114
申请日：2015-10-23
IPC分类号： A61K31/4706 , A61K45/06 , A61K31/131
CPC分类号： A61K31/4706 , A61K31/131 , A61K45/06 , A61K2300/00
摘要： Compositions and methods including Amodiaquine (AQ) or N-Desethy Amodiaquine (DEAQ) are provided for treating, inhibiting, or preventing cathepsin B dependent pathogens and toxins in a host cell or infected subject. Compositions and methods also include AQ or DEAQ in combination with an antibiotic for more effective clearance of the pathogen and/or toxins.
摘要翻译： 提供包括阿莫地喹（AQ）或N-去乙基阿莫地喹（DEAQ）的组合物和方法用于治疗，抑制或预防宿主细胞或感染受试者中组织蛋白酶B依赖性病原体和毒素。 组合物和方法还包括与抗生素组合的AQ或DEAQ，以更有效地清除病原体和/或毒素。
-
3.发明申请公开(公告)号：US20150024480A1
公开(公告)日：2015-01-22
申请号：US14451015
申请日：2014-08-04
发明人： Robert Doebler , Ali Nadim , James D. Sterling , Anna Hickerson , Barbara Erwin , Denice Woyski , Ryan P. Talbot , Bruce Irvine
CPC分类号： C12M47/06 , C12M23/28 , C12M27/00 , C12N1/066 , G01N1/286 , G01N1/4077 , G01N2001/4083 , G01N2001/4088 , G01N2001/4094
摘要： Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency.
摘要翻译： 摆动角度旋转包含材料的容器可能导致材料分离。 可能意外地将吸收剂或较重的物质收集得相对靠近旋转轴线收集，而较不致密或较轻的材料倾向于相对于旋转轴线收集。 沿弧形路径的振荡提供了高裂解效率。 或者，微型马达可以驱动可拆卸地容纳在容器中的叶轮。 裂解可以分批模式，流通停止或半分批模式或流通连续模式来实现。 裂解颗粒材料可能超过待裂解的材料或裂解的材料和/或空气可以基本上从室中除去以增加裂解效率。
-
公开(公告)号：US20130291965A1
公开(公告)日：2013-11-07
申请号：US13859680
申请日：2013-04-09
发明人： Anna Hickerson
IPC分类号： F16K15/00
CPC分类号： F16K15/00 , F16K15/142 , Y10T137/7837
摘要： A valve has a generally hollow core with one open end and one closed end, and at least one side port in a sidewall of the core. The valve also includes a sleeve that fits over the core and covers the side port(s). The cracking pressure of the valve is tunable by varying the parameters of the sleeve.
摘要翻译： 阀具有通常为中空的芯，其具有一个开口端和一个封闭端，以及在该芯的侧壁中的至少一个侧端口。 该阀还包括套筒，该套筒套在芯部上并覆盖侧面端口。 阀的开启压力可通过改变套筒的参数来调节。
-
5.发明申请Methods for identifying nucleotides at defined positions in target nucleic acids using fluorescence polarization 审中-公开使用荧光偏振鉴定目标核酸中定义位置的核苷酸的方法公开(公告)号：US20040038256A1
公开(公告)日：2004-02-26
申请号：US10398101
申请日：2003-07-03
发明人： Jeffrey Van Ness , David J Galas , Lori K Garrison
IPC分类号： C12Q001/68
CPC分类号： C12Q1/6858 , C12Q2561/119 , C12Q2525/131
摘要： The invention provides a method for identifying a nucleotide at a defined position in a target nucleic acid using restriction endonucleases and fluorescence polarization. The invention further provides compounds, compositions, and kits related to the method.
摘要翻译： 本发明提供了使用限制性内切核酸酶和荧光偏振来鉴定目标核酸中定义位置的核苷酸的方法。 本发明还提供了与该方法相关的化合物，组合物和试剂盒。
-
公开(公告)号：US20030165911A1
公开(公告)日：2003-09-04
申请号：US10196350
申请日：2002-07-15
IPC分类号： C12Q001/68 , C12P019/34
CPC分类号： C12Q1/6809 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , C12Q1/6844 , C12Q2600/156 , C12Q2600/158 , C40B40/06 , C12Q2525/131 , C12Q2521/313 , C12Q2565/501 , C12Q2527/107 , C12Q2525/155 , C12Q2521/301 , C12Q2531/119 , C12Q2521/513
摘要： The present invention provides methods and compositions for gene expression analyses using nicking agents.
摘要翻译： 本发明提供使用切口剂进行基因表达分析的方法和组合物。
-
公开(公告)号：US20030138800A1
公开(公告)日：2003-07-24
申请号：US10196740
申请日：2002-07-15
IPC分类号： C12Q001/68 , C12P019/34
CPC分类号： C12Q1/6809 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , C12Q1/6844 , C12Q2600/156 , C12Q2600/158 , C40B40/06 , C12Q2525/131 , C12Q2521/313 , C12Q2565/501 , C12Q2527/107 , C12Q2525/155 , C12Q2521/301 , C12Q2531/119 , C12Q2521/513
摘要： The present invention provides methods and compositions for exponential amplification of nucleic acid molecules using nicking agents. In certain aspects, the amplification may be performed isothermally. This invention is useful in many areas such as disease diagnosis.
摘要翻译： 本发明提供了使用切口剂对核酸分子进行指数扩增的方法和组合物。 在某些方面，可以等温进行扩增。 本发明在诸如疾病诊断等许多领域中是有用的。
-
8.发明授权公开(公告)号：US11447560B2
公开(公告)日：2022-09-20
申请号：US16297966
申请日：2019-03-11
摘要： Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.
-
公开(公告)号：US20200308593A1
公开(公告)日：2020-10-01
申请号：US16804305
申请日：2020-02-28
IPC分类号： C12N15/81
摘要： In accordance with the invention, isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are described. More particularly, nucleic acids isolated from Pichia pastoris wherein the nucleic acids have promoter activity are described. The invention also relates to expression methods, host cells, expression vectors, and DNA constructs, for using the Pichia pastoris promoters to produce proteins, and to the proteins produced using the expression methods.
-
公开(公告)号：US20200002663A1
公开(公告)日：2020-01-02
申请号：US16565206
申请日：2019-09-09
发明人： Robert Doebler , Ali Nadim , James D. Sterling , Anna Hickerson , Barbara Erwin , Denice Woyski , Ryan P. Talbot , Bruce Irvine
摘要： Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency.
-
-
-
-
-
-
-
-
-
搜索结果
41 条记录 (耗时 0.029 秒)
