公开(公告)号：US20200237277A1

公开(公告)日：2020-07-30

申请号：US16774909

申请日：2020-01-28

申请人： Abbott Diabetes Care Inc.

发明人： Tianmei Ouyang ,  Benjamin J. Feldman ,  Hyun Cho ,  Lam N. Tran ,  Stephen Oja ,  Mark K. Sloan ,  Ashwin Kumar ,  Namvar Kiaie ,  Michael R. Love

IPC分类号： A61B5/1486 ,  C12Q1/30 ,  C12Q1/32 ,  A61B5/145 ,  B60K28/06

摘要： Multiple enzymes may be present in one or more active areas of an electrochemical analyte sensor for detecting one or more different analytes. In particular, an analyte sensor may comprise a sensor tail configured for insertion into a tissue and one or more working electrodes having a glucose-responsive active area and an ethanol-responsive active area to detect glucose and ethanol in vivo.

公开(公告)号：US10704029B2

公开(公告)日：2020-07-07

申请号：US16059096

申请日：2018-08-09

申请人： Roche Diagnostics Operations, Inc. ,  Roche Diabetes Care, Inc.

发明人： Daniela Beck ,  Mara Boenitz-Dulat ,  Peter Kratzsch ,  Thomas Streidl ,  Carina Horn

IPC分类号： C12N9/02 ,  C12N9/04 ,  C12Q1/32 ,  C12P7/18

摘要： The present invention relates to a mutant 3-hydroxybutyrate dehydrogenase (3-HBDH) with improved performance relative to the wild-type 3-HBDH, a nucleic acid encoding the mutant 3-HBDH, a cell comprising the mutant 3-HBDH or the nucleic acid, a method of determining the amount or concentration of 3-hydroxybutyrate in a sample, and a device for determining the amount or concentration of 3-hydroxybutyrate in a sample.

公开(公告)号：US10690657B2

公开(公告)日：2020-06-23

申请号：US14439207

申请日：2013-10-29

申请人： Nederlandse Organisatie voor toegepast-natuurwetenschappelijk onderzoek TNO

发明人： Helena Maria Wortelboer ,  Dimitri Grossouw

IPC分类号： C12Q1/32 ,  G01N33/50 ,  C12M1/12 ,  B01L3/00 ,  C12M3/00

摘要： A novel vial for holding a segment of epithelial tissue is provided. The vial is easy to assemble and allows horizontal alignment of the tissue sample. A device comprising the vial, to methods for generating the device, and to a multitude of said devices which allow medium throughput measurements of absorption, transport and/or secretion across an epithelial tissue are also provided.

公开(公告)号：US10669564B2

公开(公告)日：2020-06-02

申请号：US15746609

申请日：2016-07-22

申请人： Smartzyme Biopharma Ltd.

发明人： Chen Haim Guttman ,  David Baram ,  Itamar Oz Gofberg ,  Dotan Omer

IPC分类号： C12Q1/00 ,  C12Q1/32 ,  C12N9/04 ,  A61B5/145 ,  A61B5/1486 ,  C12N9/06

摘要： In some embodiments, the present invention a mutated FAD-GDHα protein, wherein the mutated FAD-GDHα protein is mutated from a wild-type first species to contain at least one point mutation, wherein the mutated FAD-GDHα protein comprises: P(X)n=8X4(X)n=16V(X)n=6RN(X)n=3YDXRPXCXGX3NNCMP(X)n=1CP(X)n=2A(X)n=1Y(X)n=1G(X)n=6A(X)n=2AG(X)n=6AVV(X)n=3E(X)n=8-9A(X)n=2Y(X)n=1D(X)n=5HRV(X)n=5V(X)n=2A(X)n=3E(X)n=2K(X)n=4S(X)n=5P(X)n=1G(X)n=2N(X)n=4GRN(X)n=1MDH(X)n=4V(X)n=1F(X)n=6-7W(X)n=1GRGP(X)n=9RDGXX5R(X)n=19T(X)n=14L(X)n=14X2(X)n=1X1(X)n=1E(X)n=4P(X)n=1NR(X)n=3S(X)n=4D(X)n=2G(X)n=7Y(X)n=4Y(X)n=32-35, wherein each X represents a wild-type amino acid residue of the first species and n indicates the number of the wild-type amino acid residues of the first species represented by a respective parenthetical at that position, wherein: a) X1 is selected from the group consisting of X, S, C, T, M, V, Y, N, P, L, G, Q, A, I, D, W, H, and E, wherein if X1 is L, H or V, then X2 is D; b) X3 is selected from the group consisting of G, H, D, Y, S, and X; c) X4 is selected from the group consisting of S and X; and d) X5 is selected from the group consisting of L and X.

公开(公告)号：US20200080956A1

公开(公告)日：2020-03-12

申请号：US16682655

申请日：2019-11-13

申请人： PHC Holdings Corporation

发明人： Motonori UCHIYAMA ,  Takuo KOUNO

IPC分类号： G01N27/327 ,  C12Q1/32 ,  C12Q1/26 ,  C12Q1/00

摘要： The present invention provides: a reagent composition having higher storage stability; a sensor involving the reagent composition; and others. According to the present invention, a specific heterocyclic compound is added to a reagent composition to improve the storage stability of the reagent composition and reduce the degree of fluctuation in current values in a sensor that utilizes reagent composition.

公开(公告)号：US10577637B2

公开(公告)日：2020-03-03

申请号：US15294374

申请日：2016-10-14

申请人： ARKRAY, Inc. ,  Ultizyme International Ltd.

发明人： Koji Katsuki ,  Hisashi Kaneda ,  Junko Shimazaki

IPC分类号： C12Q1/00 ,  G01N27/327 ,  C12Q1/32 ,  C12Q1/25 ,  C23C14/06 ,  C23C14/20 ,  C23C14/24 ,  C23C14/34 ,  C23C14/58 ,  C23C16/06 ,  C23C16/44 ,  C23C16/56 ,  C23C28/00

摘要： An enzyme electrode includes an electrode, and a detection layer which contacts the electrode and contains a crosslinking agent, an electrically conductive macromolecule and an enzyme transferring and receiving electrons to and from the electrode and does not contain an electron transfer subunit.

公开(公告)号：US20190382786A1

公开(公告)日：2019-12-19

申请号：US16452305

申请日：2019-06-25

申请人： Monsanto Technology LLC

发明人： Artem G. Evdokimov ,  Clayton T. Larue ,  Farhad Moshiri ,  Joel E. Ream ,  Xuefeng Zhou

IPC分类号： C12N15/82 ,  C12N9/02 ,  C12Q1/32

摘要： The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.

公开(公告)号：US10370677B2

公开(公告)日：2019-08-06

申请号：US15224276

申请日：2016-07-29

申请人： Monsanto Technology LLC

发明人： Artem G. Evdokimov ,  Clayton T. Larue ,  Farhad Moshiri ,  Joel E. Ream ,  Xuefeng Zhou

IPC分类号： C12N15/82 ,  C12Q1/68 ,  C12N9/02 ,  C12Q1/32

摘要： The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.

公开(公告)号：US20190231717A1

公开(公告)日：2019-08-01

申请号：US16265713

申请日：2019-02-01

申请人： Roar Holding LLC

发明人： Robert W. BROCIA

IPC分类号： A61K31/138 ,  G01N33/14 ,  C12Q1/32 ,  C12Q1/26 ,  A23L33/105 ,  C12H3/04 ,  A61K31/137 ,  G01N33/569 ,  A23L2/38

CPC分类号： A61K31/138 ,  A23L1/30 ,  A23L2/38 ,  A23L2/52 ,  A23L33/105 ,  A61K31/137 ,  A61K36/00 ,  C12H3/04 ,  C12Q1/26 ,  C12Q1/32 ,  G01N33/146 ,  G01N33/56972 ,  G01N2333/904 ,  G01N2333/90638 ,  G01N2500/02

摘要： Compositions containing monoamine oxidase inhibitors prepared by removal of alcohol from agave-derived beverages are disclosed.

公开(公告)号：US20190177699A1

公开(公告)日：2019-06-13

申请号：US16278019

申请日：2019-02-15

申请人： Ikeda Food Research Co., Ltd. ,  PHC Corporation

发明人： Hironori OMURA ,  Hirokazu SANDA ,  Takako YADA ,  Tetsunari MORITA ,  Mika KUYAMA ,  Tokuji IKEDA ,  Kenji KANO ,  Seiya TSUJIMURA

IPC分类号： C12N9/02 ,  C12Q1/32 ,  C12Q1/54

摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.