公开(公告)号：US07873478B2

公开(公告)日：2011-01-18

申请号：US11195365

申请日：2005-08-02

申请人： Detlev Suckau ,  Martin Schürenberg ,  Jochen Franzen

发明人： Detlev Suckau ,  Martin Schürenberg ,  Jochen Franzen

IPC分类号： G06F19/00 ,  G01N24/00 ,  H01J49/00

CPC分类号： G01N33/483 ,  H01J49/0004 ,  Y10T436/24

摘要： The invention relates to the determination and visualization of the spatial distribution of tissue states in histologic tissue sections on the basis of mass spectrometric signals acquired so as to be spatially resolved. The invention provides a method which determines the tissue state for the tissue spots as a state characteristic, which is calculated as a mathematical or logical expression from at least two mass signals of this tissue spot, and which indicates the tissue state as a gray-level or false-color image in one or two dimensions.

摘要翻译： 本发明涉及基于获得的空间分辨的质谱信号来确定和可视化组织组织切片中组织状态的空间分布。 本发明提供了一种方法，其将组织斑点的组织状态确定为状态特征，其从该组织斑点的至少两个质量信号计算为数学或逻辑表达，并且将组织状态指示为灰度级 或一维或二维的假彩色图像。

公开(公告)号：US07872229B2

公开(公告)日：2011-01-18

申请号：US12172605

申请日：2008-07-14

申请人： Melvin A. Park ,  Michael Schubert ,  Jochen Franzen

发明人： Melvin A. Park ,  Michael Schubert ,  Jochen Franzen

IPC分类号： H01J49/42

CPC分类号： H01J49/4295 ,  H01J49/424

摘要： In a three-dimensional Paul RF ion trap at least one of the ring electrode and end cap electrodes is structured to produce a high capture efficiency for analyte ions introduced into the trap. The electrode structuring may be produced by an electrode surface profile having edges or protrusions, resulting in a scattering reflection of the introduced ions. Alternatively, at least one electrode may be formed by physically separate electrode components. In one embodiment, the trap can be switched between operating as a linear ion trap with good capture efficiency and operating as a three-dimensional ion trap with good ion reaction conditions.

摘要翻译： 在三维Paul RF离子阱中，环形电极和端盖电极中的至少一个被构造成为引入陷阱的分析物离子产生高捕获效率。 电极结构可以通过具有边缘或突起的电极表面轮廓来产生，导致引入离子的散射反射。 或者，可以通过物理分离的电极组件形成至少一个电极。 在一个实施例中，可以在具有良好捕获效率的线性离子阱之间切换陷阱，并且作为具有良好离子反应条件的三维离子阱操作。

公开(公告)号：US20100207020A1

公开(公告)日：2010-08-19

申请号：US12637184

申请日：2009-12-14

申请人： Roland Jertz ,  Gokhan Baykut

发明人： Roland Jertz ,  Gokhan Baykut

IPC分类号： H01J49/34

CPC分类号： H01J49/38

摘要： The compensation potentials on the compensation electrodes of an ICR measuring cell are sequentially adjusted so that an ICR measurement with the longest possible usable image current transient is produced. Then, subsequent ICR measurements are made using the ICR cell with the optimally adjusted compensation potentials. Depending on the kind of ion mixture involved, measurements with image current transients from 10 to more than 20 seconds long can be performed, from which mass spectra with a maximum mass resolution without peak coalescence can be obtained.

摘要翻译： 依次调整ICR测量单元的补偿电极上的补偿电位，使得产生具有尽可能长的可用图像电流瞬变的ICR测量。 然后，使用具有最佳调整的补偿电位的ICR单元进行随后的ICR测量。 根据所涉及的离子混合物的种类，可以进行从10秒到20秒以上的图像电流瞬变的测量，从而可以获得具有最大质量分辨率而不具有峰值聚结的质谱。

公开(公告)号：US07737398B2

公开(公告)日：2010-06-15

申请号：US11955581

申请日：2007-12-13

申请人： Karsten Michelmann

发明人： Karsten Michelmann

IPC分类号： H01J49/42 ,  H01J49/00

CPC分类号： H01J49/025 ,  H01J49/423

摘要： In a linear ion trap in which an essentially quadrupole RF electrical field is generated between at least four rod-shaped electrodes, ions may be mass-selectively ejected orthogonally to the axis. An aspect of the invention comprises compensating for field irregularities along the axis of a linear ion trap, which result, at different ejection locations, in the ejection of ions of the same masses at slightly different times, by of measuring the ions that are ejected at the different ejection locations using a number of separate detectors, and correcting, after a mass calibration of each of the mass spectra, the time shifts of the various location-dependent mass spectra during their addition to a combined spectrum.

摘要翻译： 在其中在至少四个棒状电极之间产生基本上四极杆RF电场的线性离子阱中，离子可以被质量选择性地垂直于该轴线喷射。 本发明的一个方面包括补偿沿着线性离子阱的轴的场不规则，其导致在不同的喷射位置，在稍微不同的时间喷射同一质量的离子，通过测量在 使用多个单独的检测器的不同的喷射位置，并且在每个质谱的质量校准之后校正在添加到组合光谱期间各种位置依赖质谱的时间偏移。

公开(公告)号：US20100140466A1

公开(公告)日：2010-06-10

申请号：US12617367

申请日：2009-11-12

申请人： Ralf Hartmer

发明人： Ralf Hartmer

IPC分类号： H01J49/26 ,  B01D59/44

CPC分类号： G01N33/6848 ,  H01J49/0072

摘要： Radical anions for use in the fragmentation of positively charged biopolymer ions by means of electron transfer are produced from substances previously unknown for use as ETD production substances. The inventive substances produce radical anions that lead to electron transfer dissociations with a high yield of fragment ions. The substances have high volatility that allows them to be kept in unheated containers outside the vacuum system and transported into the vacuum system to an in vacuum electron attachment ion source via unheated lines and low molecular weights that allow the measurement of even very light fragment ions. In one embodiment, a suitable substance is 1-3-5-7-cyclooctatetraene.

摘要翻译： 用于通过电子转移使带正电荷的生物聚合物离子分解的自由基阴离子由以前未知的用作ETD生产物质的物质产生。 本发明的物质产生导致电子转移离解的自由基阴离子，具有高产率的碎片离子。 这些物质具有高挥发性，使其能够保持在真空系统外部的未加热的容器中，并通过未加热的管线和低分子量输送到真空电子附着离子源，允许测量甚至非常轻的碎片离子。 在一个实施方案中，合适的物质是1-3-5-7-环辛四烯。

公开(公告)号：US20100090102A1

公开(公告)日：2010-04-15

申请号：US12548945

申请日：2009-08-27

申请人： Oliver Räther ,  Gökhan Baykut ,  Jochen Franzen

发明人： Oliver Räther ,  Gökhan Baykut ,  Jochen Franzen

IPC分类号： B01D59/44 ,  H01J49/00

CPC分类号： H01J49/062 ,  G01N27/622

摘要： Ion mobilities are measured by entraining the ions in a gas and adiabatically expanding the ion-containing gas through a nozzle to form a gas jet. An electrical field barrier with variable height is located at the nozzle exit. The field barrier may be located adjacent to the nozzle exit or an ion guide may be located between the nozzle and the field barrier. If a continuous ion current is supplied, the height of the barrier is varied and the ion current of the ions passing over the barrier is measured, the ion current can be differentiated to generate a mobility spectrum. Alternatively, the ions can be temporarily stored in the ion guide so that measurement of the ion current of the ions passing over the barrier results in a direct measurement of the mobility spectrum.

摘要翻译： 通过夹带气体中的离子并通过喷嘴绝热地使含离子的气体膨胀以形成气体射流来测量离子迁移率。 具有可变高度的电场屏障位于喷嘴出口处。 场屏障可以位于喷嘴出口附近，或者离子引导件可以位于喷嘴和场屏障之间。 如果提供连续的离子电流，则屏障的高度变化，并且测量通过势垒的离子的离子电流，离子电流可以被微分以产生迁移谱。 或者，离子可以临时存储在离子导向器中，使得通过势垒的离子的离子电流的测量导致对迁移谱的直接测量。

公开(公告)号：US20090084948A1

公开(公告)日：2009-04-02

申请号：US11957613

申请日：2007-12-17

申请人： Gokhan Baykut ,  Roland Jert

发明人： Gokhan Baykut ,  Roland Jert

IPC分类号： B01D59/44

CPC分类号： H01J49/38 ,  G06Q10/0633 ,  H01J49/4265

摘要： In an ion cyclotron resonance mass spectrometer in which ions are trapped axially by applying electrical potentials to a pattern of electrode elements to produce an inhomogeneous alternating radio-frequency electric field with a repulsive effect, an additional electrostatic ion-attracting field is superimposed on the repulsive electric field. The voltage of the ion-attracting field is adjusted to compensate for a cyclotron frequency shift of the ions caused by the ion space charge. The voltage of the ion-attracting field can be adjusted so that the ion cyclotron frequency of all ions becomes independent of the number of ions inside the spectrometer.

摘要翻译： 在离子回旋共振质谱仪中，通过将电位施加到电极元件的图案以产生具有排斥效应的不均匀的交变射频电场，其中离子被轴向捕获，附加的静电离子吸引场叠加在排斥 电场。 调整离子吸引场的电压以补偿由离子空间电荷引起的离子的回旋加速器频移。 可以调节离子吸引场的电压，使得所有离子的离子回旋加速器的频率独立于光谱仪内的离子数。

公开(公告)号：US07504640B2

公开(公告)日：2009-03-17

申请号：US11522112

申请日：2006-09-15

申请人： Jochen Franzen ,  Karsten Michelmann

发明人： Jochen Franzen ,  Karsten Michelmann

IPC分类号： H01J27/00 ,  H01J49/04 ,  B01D59/44

CPC分类号： G01N27/62 ,  H01J49/0463 ,  H01J49/0481 ,  H01J49/145

摘要： An ion source generates ions from analyte molecules which are desorbed from a sample on the surface of a sample support in a pressure range of approximately 30 to 300 pascal. Reactant ions are generated in a separate ion source and guided by ion guides to the point in front of the sample or to a reaction chamber in which the desorbed molecules are located. The reactant ions ionize the desorbed molecules to form analyte ions. The analyte molecules can be mixed in matrix material or adsorbed on the sample support surface without additives. The desorption can be continuous or pulsed, for example by light from lasers or diodes.

摘要翻译： 离子源从分析物分子产生离子，其在约30至300帕斯卡的压力范围内从样品载体表面上的样品解吸。 反应物离子在单独的离子源中产生并由离子导向器引导到样品前面的点或解吸的分子位于其中的反应室。 反应物离子离子解吸分子形成分析离子。 分析物分子可以在基质材料中混合或吸附在样品支持体表面上而无添加剂。 解吸可以是连续的或脉冲的，例如通过来自激光器或二极管的光。

公开(公告)号：US20090039282A1

公开(公告)日：2009-02-12

申请号：US12177434

申请日：2008-07-22

申请人： Andreas Haase ,  Jens Hohndorf

发明人： Andreas Haase ,  Jens Hohndorf

IPC分类号： H01J27/02 ,  B01D59/44

CPC分类号： H01J49/164

摘要： Analyte ions are generated in an ion source by matrix-assisted laser desorption (MALDI) in which laser light pulses have significantly less than one nanosecond duration, focal diameters of less than twenty micrometers and energy densities such that only about one picogram of sample is desorbed per pulse of laser light and per laser spot. An unexpectedly high degree of ionization of analyte molecules is produced for selected matrix substances. Many laser spots can be generated side-by-side from a single laser light pulse for use with MALDI time-of-flight mass spectrometers. Applying pulses with a repetition rate of around 50 kilohertz and moving the sample or guiding the laser light beam so each laser light pulse impinges on a cool sample spot allows the ion source to be used with spectrometers that require a constant ion current.

摘要翻译： 分析离子通过基质辅助激光解吸（MALDI）在离子源中产生，其中激光脉冲具有显着小于一纳秒的持续时间，焦点直径小于20微米，能量密度使得仅约一个皮克的样品解吸 每个激光脉冲和每个激光点。 对于选定的基质物质产生了分析物分子意外高度的电离。 可以从单个激光脉冲并排生成许多激光点，以便与MALDI飞行时间质谱仪一起使用。 施加重复频率约为50千赫兹的脉冲，并移动样品或引导激光束，使每个激光脉冲入射到一个较冷的样品点上，使离子源与需要恒定离子电流的光谱仪一起使用。

公开(公告)号：US20080251715A1

公开(公告)日：2008-10-16

申请号：US12056630

申请日：2008-03-27

申请人： Evgenij Nikolaev ,  Jochen Franzen

发明人： Evgenij Nikolaev ,  Jochen Franzen

IPC分类号： H01J49/26

CPC分类号： G21K1/093 ,  H01J49/065 ,  H01J49/38

摘要： In a mass spectrometer that uses a space-restricted magnetic field, such as an ion cyclotron resonance mass spectrometer, ions with a wide mass range generated in an ion supply located outside the magnetic field are transported in the direction of the magnetic field lines to an ion storage device located inside the magnetic field without losing ions by guiding the ions through the region in which the magnetic field strength increases with a special ion guide. This ion guide consists of an arrangement of coaxial ring diaphragms which are alternately supplied with the phases of an RF voltage. In an alternative embodiment, the ion guide uses two wires wound in a double helix where each wire is supplied with one phase of a two-phase RF voltage.

摘要翻译： 在使用诸如离子回旋共振质谱仪的空间受限的磁场的质谱仪中，在位于磁场外部的离子供体中产生的质量范围宽的离子在磁场线的方向上被传送到 离子存储装置，其位于磁场内部，而不会通过引导离子通过特殊离子导向器的磁场强度增加的区域而损失离子。 该离子导向器由交替地提供有RF电压的相位的同轴环形隔膜的布置组成。 在替代实施例中，离子导向器使用缠绕在双螺旋线中的两条线，其中每条线被提供有两相RF电压的一相。