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    PRE-ANCHOR WASH
    41.
    发明申请
    PRE-ANCHOR WASH 审中-公开
    预紧器清洗

    公开(公告)号：US20130296173A1

    公开(公告)日：2013-11-07

    申请号：US13868000

    申请日：2013-04-22

    Applicant: Complete Genomics, Inc.

    Inventor： Matthew Callow ,  Linsu Chen ,  Dennis Ballinger

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12Q2527/125

    Abstract: The present invention is directed to compositions and methods for improving the discordance rate and mapping yield in nucleic acid sequencing reactions.

    Abstract translation: 本发明涉及用于提高核酸测序反应中不一致率和测绘产率的组合物和方法。

    Nucleic acid analysis by random mixtures of non-overlapping fragments
    46.
    发明授权
    Nucleic acid analysis by random mixtures of non-overlapping fragments 有权

    公开(公告)号：US11414702B2

    公开(公告)日：2022-08-16

    申请号：US16730829

    申请日：2019-12-30

    Applicant: Complete Genomics, Inc.

    Inventor： Radoje Drmanac

    IPC: C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/682 ,  C12Q1/6837 ,  C07H21/04 ,  C07K1/04 ,  G01N15/14

    Abstract: The invention provides methods and kits for ordering sequence information derived from one or more target polynucleotides. In one aspect, one or more tiers or levels of fragmentation and aliquoting are generated, after which sequence information is obtained from fragments in a final level or tier. Each fragment in such final tier is from a particular aliquot, which, in turn, is from a particular aliquot of a prior tier, and so on. For every fragment of an aliquot in the final tier, the aliquots from which it was derived at every prior tier is known, or can be discerned. Thus, identical sequences from overlapping fragments from different aliquots can be distinguished and grouped as being derived from the same or different fragments from prior tiers. When the fragments in the final tier are sequenced, overlapping sequence regions of fragments in different aliquots are used to register the fragments so that non-overlapping regions are ordered. In one aspect, this process is carried out in a hierarchical fashion until the one or more target polynucleotides are characterized, e.g. by their nucleic acid sequences, or by an ordering of sequence segments, or by an ordering of single nucleotide polymorphisms (SNPs), or the like.

    METHODS AND COMPOSITIONS FOR NUCLEIC ACID SEQUENCING
    47.
    发明申请
    METHODS AND COMPOSITIONS FOR NUCLEIC ACID SEQUENCING 有权

    公开(公告)号：US20220186309A1

    公开(公告)日：2022-06-16

    申请号：US17407935

    申请日：2021-08-20

    Applicant: Complete Genomics, Inc.

    Inventor： Radoje Drmanac

    IPC: C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/686

    Abstract: The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences. In particular, the present invention provides methods and compositions for improving the efficiency of sequencing reactions by using fewer labels to distinguish between nucleotides and by detecting nucleotides at multiple detection positions in a target sequence.

    TREATMENT FOR STABILIZING NUCLEIC ACID ARRAYS
    48.
    发明申请
    TREATMENT FOR STABILIZING NUCLEIC ACID ARRAYS 有权

    公开(公告)号：US20210131924A1

    公开(公告)日：2021-05-06

    申请号：US17094523

    申请日：2020-11-10

    Applicant: Complete Genomics, Inc.

    Inventor： Norman Lee Burns ,  Jay Willis Shafto

    IPC: G01N1/30 ,  B01J19/00

    Abstract: The present invention is directed to treatment of nucleic acid molecules that are attached or associated with solid supports for biochemical analysis, including nucleic acid sequencing. After loading on the solid support, the nucleic acid molecules are treated with a composition comprising a condensing agent, a volume excluding agent, or both, then treated with a composition comprising a protein.

    Water level sequencing flow cell fabrication
    50.
    发明授权
    Water level sequencing flow cell fabrication 有权

    公开(公告)号：US10784103B2

    公开(公告)日：2020-09-22

    申请号：US16128120

    申请日：2018-09-11

    Applicant: Complete Genomics, Inc.

    Inventor： Shifeng Li ,  Jian Gong ,  Yan-You Lin ,  Cheng Frank Zhong

    IPC: H01L21/02 ,  H01L29/20 ,  H01L21/28 ,  G01N21/64 ,  G01N21/05 ,  G01N33/487 ,  B01L3/00

    Abstract: A method for forming sequencing flow cells can include providing a semiconductor wafer covered with a dielectric layer, and forming a patterned layer on the dielectric layer. The patterned layer has a differential surface that includes alternating first surface regions and second surface regions. The method can also include attaching a cover wafer to the semiconductor wafer to form a composite wafer structure including a plurality of flow cells. The composite wafer structure can then be singulated to form a plurality of dies. Each die forms a sequencing flow cell. The sequencing flow cell can include a flow channel between a portion of the patterned layer and a portion of the cover wafer, an inlet, and an outlet. Further, the method can include functionalizing the sequencing flow cell to create differential surfaces.

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